Objective: To study the anti-tumor constituents from the roots of Actinidia valvata Dunn. Methods: Column chromatographic techniques were used for the isolation and purification of chemical constituents of the plant and the structures were elucidated by spectroscopic analysis. The cytotoxic activity was tested by MTT methods. Results: Ten triterpenoids: 2, 3, 24 – trihydroxyurs – 12 – en – 28 – oic acid (1), asiatic acid (2), corosolic acid (3), 2, 3, 23, 24 – tetrahydroxyurs – 12 – en – 28 – oic acid (4), 2, 3, 13, 24 – tetrahydroxyurs – 11 – en – 28 - oic acid – 13 – lactone (5), 2, 3, 24 – trihydroxyolean – 12 – en – 28 – oic acid (6), 2, 3, 19, 24 – tetrahydroxyurs – 12 – en – 28 – oic acid (7), 2, 3, 24 – trihydroxyurs – 12, 20 (30) – dien – 28 - oic acid (8), 2, 3, 24 – trihydroxyurs – 12 – en – 28 – oic acid (9), olean acid (10), and  – sitosterol (11) were isolated from the CHCl3 fraction of this plant, respectively. The cytotoxic activity of five compounds (1, 2, 3, 4, 7) against A549, LOVO and HepG2 cell lines were evaluated. Conclusion: Compounds 3-8 are isolated from this plant for the first time. Compounds 2 and 3 showed weakly cytotoxic activity against LOVO and HepG2 cell lines, compared to DOX as a positive control.