ObjectiveTo investigate the changes of peripheral pain threshold after knockout of transient receptor potential vanilloid 1(TRPV1) gene. MethodsTail-Flick Analgesia Meter and von-Frey hair were used to determine the peripheral thermal and mechanical thresholds in TRPV1 knockout and wild-type female mice, and the results of the two groups were compared. ResultsThe Tail-Flick latency in TRPV1 knockout mice was significantly prolonged after hot stimulation compared with that in the wild-type group(\[3.59±0.65\] s vs \[2.19±0.24\] s, P<0.05).There was no significant change in responses to von-Frey hair stimuli between the two groups(\[1.71±0.57\] g vs \[2.13±0.81\] g,P>0.05). ConclusionIt is suggested that TRPV1 receptor mediate thermal stimuli response under physiological condition, and has no noticeable influence on mechanical stimuli response.