Objective To present our initial experience of single-port multi-channel laparoscopic urologic surgery for the transumbilical laparoscopic implantation of long-term peritoneal dialysis catheters. Methods On May 17th and August 18th ,2009, 2 patients underwent single-port multi-channel laparoscopic surgery for chronic renal failure (CRF) due to benign arteriolonephrosclerosis and type 2 diabetes mellitus(DM). The flexible laparoscopic instrumentation with a homemade single port and multichannels was used for the transperitoneal approach. Results The 2 cases were completed without conversion to standard laparoscopic or laparotomy. The operation time for implantation was 15-25 min, and the estimated blood loss was about 20 ml. The patients were discharged 1 day after operation and were followed up for 6-8 months. Conclusion Single-port multi-channel laparoscopy is feasible, safe, and effective for transumbilical laparoscopic implantation of long-term peritoneal dialysis catheters, with the advantages of minimal incision and better cosmetic benefit.