ObjectiveTo investigate the factors influencing condom use among spouses/fixed partners of HIV-infected people. MethodsThe spouses/fixed partners of HIV-infected people were selected for questionnaire investigation. Rank sum test and logistic regression analysis were employed to study the factors influencing the use of condom among them. ResultsTotally 395 qualified subjects were recruited, and 72.66% of them used condoms every time when they had sexual behavior and 15.70% never used condom. The results of the logistic regression analysis showed that the factors influencing condom use among the spouses/fixed partners of HIV-infected people included ethnicity, having children or not, knowledge about HIV/AIDS, time when HIV was detected in spouse and self-awareness of the possibility to catch HIV from their spouses/fixed partners. ConclusionCondom use among the spouses/fixed partners of HIV-infected people is not satisfactory in China, which is due to multiple factors. More efforts need to be made to enforce AIDS intervention among target population. High-risk groups should be screened on a regular basis and timely measures should be taken.