Thyroid hormone preconditioning alleviates reperfusion-induced renal inflammation in mice
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Supported by Grant from Shanghai Science and Technology Committee (09410706300).

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    Abstract:

    Objective To investigate the influence of thyroid hormone T3 preconditioning on interleukin-10 (IL-10) and interleukin-1 receptor antagonist (IL-1Ra) modulation and neutrophil infiltration after renal shchemia/reperfusion (IR) in mice, so as to study the protective effect of T3 on IR kidney. Methods Totally 120 male C57BL/6 mice were randomly divided into four groups (n=30), namely, control group (sham operation), IR group (only received renal IR), T3+IR group (T3 preconditioning for 48 h before renal IR), and NaOH+IR group (received equivalent 0.1 mol/L NaOH soltuion 48 h before renal IR). The serum creatinine and blood urea nitrogen (BUN) were determined 24 h after reperfusion in each group; renal histological damages were scored using PAS staining; the levels of neutrophil infiltration were evaluated by MPO staining, and IL-10, IL-1Ra mRNA expression was examined by real-time PCR at 1, 3, 6, 12, 24, and 48 h after reperfusion. Results The serum creatinine and BUN levels of T3+IR group were significantly lower than those of IR group 24 h after reperfusion (P<0.05), which was accompanied by lower histological score and significantly less neutrophil infiltration (P<0.05). Real-time PCR results showed that IL-10 and IL-1Ra mRNA expression in T3+IR group was significantly higher than that in the IR group (P<0.05) 12 h after reperfusion, which lasted for 48 h after reperfusion. The above parameters were similar between IR group and NaOH+IR group. onclusion Thyroid hormone T3 preconditioning can alleviate renal IR injury, partly by increasing expression of IL-10 and IL-1Ra and subsequently reducing neutrophil infiltration at the late phase of renal IR.

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History
  • Received:February 13,2012
  • Revised:March 22,2012
  • Adopted:March 30,2012
  • Online: April 24,2012
  • Published:
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