Objective To establish a high performance liquid chromatography approach for determining matrine and sophoridine contents in Ganlibao capsule. MethodsAgilent NH2 (4.6 mm×250 mm, 5 μm) column was used with 1% phosphoric acidethanolacetonitrile (V/V/V=102268) as the mobile phase. The flow rate was 0.8 ml/min, and the temperature was at 25℃.The detection wavelength was set at 210 nm. Results The calibration curve of matrine showed a good linearity in the range of 5.21-156.3 μg/ml (r=1) and that of sophoridine in the range of 5.29-158.7 μg/ml (r=0.999 9). The equations of linear regression of matrine and sophoridine were Y=9.586 1X+1.003 8 and Y=7.604 3X-5.920 1, respectively. The recovery was 98.73% (RSD=3.67%) for matrine and 100.10% (RSD=2.24%) for sophoridine. The matrine content of 15 batches of Ganlibao capsule was (30.13±0.19)-(44.70±0.17) mg/g and the sophoridine content was (21.60±0.25)-(52.07±0.11) mg/g. Conclusion The present method is simple, accurate, and reproducible, and it can be used for the quality control of Ganlibao capsule.