Objective To establish a method for determining the contents of ursolie acid and oleanolic acid in Qinggansanjie granules for quality control of the preparation. MethodsHigh-performance liquid chromatography with a diode-array detector (HPLC-DAD) was used for determination at the following conditions: The column was Chromsil-C18 (4.6 mm×250 mm, 5 μm), mobile phase was A: 0.1% (V/V) formic acid solution, B: methanol, A∶B = 12∶88. The flow rate was 1.0 ml/min, the temperature of column was 25℃, the detection wavelength was set at 210 nm, the injection volume was 50 μl, and the running time was 25 min. Results Ursolic acid and oleanolic acid could be separated on baseline, with the linear range being 6.1-97.6 μg/ml for ursolic acid and 6.4-102 μg/ml for oleanolic acid. The results of intra-day and inter-day precisions were both within 2% (n=3), and the average recovery was 95.4% (RSD 1.54%, n=6) and 99.2% (RSD 1.97%, n=6). Conclusion The present method is simple, rapid, accurate, and with satisfactory repeatability; it can be used for quality control of ursolic acid and oleanolic acid in Qinggansanjie granules.