Objective To investigate the chemical components of gorgonian Menella kanisa collected from the South China Sea. Methods The Et₂O extract of gorgonian Menella kanisa was purified by repeated column chromatography on silica gel, Sephadex LH-20, and reversed-phase high-performance liquid chromatography (RP-HPLC). The structures of the obtained compounds were determined on the basis of spectroscopic analysis and compared with the reported data. Results and Conclusion Five 5α,8α-epidioxy sterols were isolated from gorgonian Menella kanisa collected from the South China Sea, and their structures were determined as: 5α,8α-epidioxy-cholest-6-en-3β-ol (1), (22E)-5α,8α-epidioxy-cholesta-6,22-dien-3β-ol (2), (22E,24R)-5α,8α-epidioxy-24-methyl-cholesta-6,22-dien-3β-ol (3), 5α,8α-epidioxy-24-methyl-cholesta-6,24(28)-dien-3β-ol (4), (24E)-5α,8α-epidioxy-24-ethyl-cholesta-6,24(28)-dien-3β-ol (5). All the five epidioxy sterols have been separated from the title Menella kanisa for the first time.