Objective To investigate the sleep quality and personality of staffs suffering long-term chronic stress. Methods A total of 100 staffs working in a showcase unit in Shanghai for over 1 year (with long-term chronic stress) were assessed with Pittsburgh sleep quality index (PSQI), Eysenck personality questionnaire (EPQ) and self-rating symptom scale (SCL-90). The sleep quality and mental health status were compared between staffs with different personalities. Results Totally 97 valid questionnaires were collected and the valid response rate was 97%. (1) Under long-term chronic stress the mean score of PSQI was (2.76±1.85) for the 97 staffs, and 18.6% (18/97) of them had sleeping disorders (PSQI ≥5). Staffs of different genders, ages, educational backgrounds and seniorities showed no significant difference in sleeping disorder incidence (P>0.05). (2) The psychoticism and neuroticism scores of EPQ in the 97 staffs were significantly lower than the norms, while their extraversion-introversion dimension of EPQ was significantly higher than the norms (P<0.05). (3) The emotion stable staffs (66) had significantly lower PSQI and SCL-90 scores compared with the intermediate staffs (31, P<0.05); the extravert staffs (72) also had significantly lower PSQI and SCL-90 scores compared with the intermediate staffs (25, P<0.05). (4) Compared with the norms, the total scores and the scores of somatization, obsessive-compulsive, interpersonal relationship, depression, anxiety, hostility, phobic anxiety, and paranoid ideation of SCL-90 were significantly lower in staffs under long-term chronic stress (P<0.05). (5) It showed that the score of extraversion-introversion dimension of EPQ was negatively correlated with the scores of obsessive-compulsive, interpersonal relationship, depression, anxiety, phobic anxiety, paranoid ideation and psychoticism factors of SCL-90, while the neuroticism score of EPQ was positively correlated with all factors of SCL-90 (P<0.05). Conclusion The prevalence of sleep disorder of staffs under the long-term chronic stress is 18.6% in our study. The personality of staffs under the long-term chronic stress is extravert and emotionally stable. Staffs with extravert personality and stable type are less likely to have psychological and sleep quality problems.