Objective To quantitatively analyze the changes of ovarian artery blood flow volume during whole pulsation cycle in patients with polycystic ovary syndrome (PCOS) by pulsed Doppler. Methods The ovarian arteries of 80 PCOS patients were examined by transvaginal color Doppler flow image (TV-CDFI). After examination of the parameters of pulsed Doppler, the ovarian artery blood flow volume in whole pulsation cycle was calculated with velocity-time integral while considering the internal diameter of the blood vessels. The results were compared with those of 80 healthy women of childbearing age in the control group. Results The typical spectrum of ovarian artery blood flow in PCOS patients was high-speed low-resistance waveform. The changes of blood flow volume and resistance index (RI) value were not significantly different at different times of the menstrual cycle (P>0.05). However, the changes of blood flow volume and RI value in the control group were significanly different at different times of the menstrual cycle (P<0.05). The blood flow volume of PCOS patients was significantly increased compared with those of the control group at different times of menstrual cycle (P<0.05). The RI value of ovarian artery in PCOS patients was significantly lower than those in the control group during menstrual cycle 3-9 days and 26-32 days (P<0.05), but showing no significant difference during menstrual cycle 11-15 days and 18-23 days (P>0.05). Conclusion Quantitative determination of ovarian blood flow volume using blood flow whole pulsation period method can provide a relatively accurate and reliable quantitative assessment of the ovarian perfusion in PCOS patients.