Colon cancer stem cells(CCSCs)play key roles in tumor initiation, proliferation,metastasis,and chemoresistance. Currently a large number of reports focused on markers of CCSCs, with relative less reporting on CCSCs biological characteristics: proliferation, metastasis, and chemoresistance. This paper reviewed the current research on the biological behavior of CCSCs, with special attention paid on the regulatory effects of microRNAs on CCSCs, hoping to provide reference for improving the diagnosis and treatment of colon cancer.