Objective To explore the feasibility and effectiveness of establishing an aortic valve regurgitation canine model by minimally invasive peripheral vascular catheter method. Methods A total of 10 healthy canines were selected in this study. An aortic insufficiency animal model was established by percutaneous femoral artery puncture and aortic dilatation. Aortic angiography and transthoracic echocardiography were used to evaluate aortic valve regurgitation immediately after operation. Results Aortic valve regurgitation animal models were successfully established in all the 10 canines. Two canines died of acute left ventricular failure due to the large aortic regurgitation after the operation. Six surviving canines had moderate aortic regurgitation and two had slight aortic regurgitation; they survived for more than 1 month. Conclusion It is feasible to establish an aortic regurgitation canine model by minimally invasive peripheral vascular catheter method.