Effect of electret electrostatic-field on preparing nanopaticles carrying quaternary ammonium chitosan and epidermal growth factor
CSTR:
Author:
Affiliation:

Department of Physics and Mathematics,College of Basic Medical Sciences,Second Military Medical University,Shanghai,China Department of Inorganic Chemistry,School of Pharmacy,Second Military Medical University The medical department,Second Military Medical University,Shanghai,China,,Department of Physics and Mathematics,College of Basic Medical Sciences,Second Military Medical University,Shanghai,China Department of Inorganic Chemistry,School of Pharmacy,Second Military Medical University The medical department,Second Military Medical University,Shanghai,China,Department of Physics and Mathematics,College of Basic Medical Sciences,Second Military Medical University,Shanghai,China Department of Inorganic Chemistry,School of Pharmacy,Second Military Medical University The medical department,Second Military Medical University,Shanghai,China,,Department of Physics and Mathematics,College of Basic Medical Sciences,Second Military Medical University,Shanghai,China Department of Inorganic Chemistry,School of Pharmacy,Second Military Medical University The medical department,Second Military Medical University,Shanghai,China,Department of Physics and Mathematics,College of Basic Medical Sciences,Second Military Medical University,Shanghai,China Department of Inorganic Chemistry,School of Pharmacy,Second Military Medical University The medical department,Second Military Medical University,Shanghai,China

Clc Number:

Fund Project:

Supported by National Natural Science Foundation of China (51477175), Military Project of Second Military Medical University (2013JSD2), and Innovation Training Program of College Students of Second Military Medical University (PH2014003).

  • Article
  • |
  • Figures
  • |
  • Metrics
  • |
  • Reference
  • |
  • Related
  • |
  • Cited by
  • |
  • Materials
  • |
  • Comments
    Abstract:

    Objective To study the effect of electret electrostatic-field on the preparation and characteristics of nanopaticles carrying quaternary ammonium chitosan and epidermal growth factor (EGF), so as to explore the percutaneous transport mechanism of drug-loading nanoparticles. Methods Under different electret electrostatic-fields, ionic crossing method was used to prepare EGF-loading chitosan nanoparticles using EGF as model drug, chitosan as material, and sodium tripolyphosphate(TPP) as crosslinking agent. Transmission electron microscope was employed to observe the surface morphology of nanoparticles; the particle size and the zeta potential of the EGF-loading nanoparticles were also examined. Results (1) The EGF-loading chitosan nanoparticles prepared in this study had uniform distribution, good dispersibility and stability. (2) The size of the EGF-loading nanoparticles increased with the increase of chitosan concentration, indicating that chitosan concentration might be the main reason for the nanoparticle size. (3) The particle size of the EGF-loading chitosan nanoparticles decreased with the increase of electrostatic field; and the change of the zeta potential was closely related to the polarity of the applied electric field. (4) The particle size of EGF-loading nanoparticles decreased with the increase of storage time. Conclusion The morphology, particle size and zeta potential of chitosan nanoparticles are closely related to the intensity and polarity of the applied electric field; proper electric field parameters should be chosen to prepare high quality chitosan nanoparticles with appropriate particle size and stability.

    Reference
    Related
    Cited by
Related Videos

Share
Article Metrics
  • Abstract:
  • PDF:
  • HTML:
  • Cited by:
History
  • Received:March 06,2016
  • Revised:April 28,2016
  • Adopted:June 24,2016
  • Online: July 27,2016
  • Published:
Article QR Code