Objective To observe the expression of clasmatoblast-secreted tryptase in patients with diabetic cardiomyopathy (DCM) and its relationship with fasting blood glucose. Methods Patients with DCM (DCM group, n=32) or ischemic cardiomyopathy (ICM group, n=26) and age-matched healthy volunteers (healthy control group, n=20) were recruited from Changzheng Hospital of Second Military Medical University between August 2012 and March 2013. The clinical characteristics of the participants were collected and the biochemical indices including fasting blood-glucose were detected. ELISA detection reagent kit was used for detecting serum tryptase level in each group. Pearson analysis was used to analyze the correlation between plasma tryptase level and fasting glucose. Results The general clinical characteristics and the fasting blood glucose were not significantly different between the three groups. The plasma tryptase levels in DCM group and ICM group were significantly higher than that in the healthy control group ([7.19±0.62] μg/L,[6.81±0.94] μg/L vs[2.37±0.56] μg/L, P<0.01). DCM group had higher serum tryptase level than ICM group, but with no significant difference. Pearson analysis showed that the plasma tryptase level was positively correlated with fasting blood glucose level (r=0.637, P<0.01). Conclusion The plasma tryptase level is increased in patients with DCM, and it is positively correlated with the fasting blood glucose in all the participants of three groups.