Objective To detect the relaxation property of mesoporous silica nanocapsules loaded liposomes (MSN-LIPO), and to explore their functions of enhancing MRI T₂ weighted imaging. Methods In vitro, MSN-LIPO with different iron concentrations were scanned by MRI system to detect their relaxation properties. In vivo, six BALB/c nude mouse models with subcutaneous malignant glioma were established and randomly divided into two groups, which were injected with the same concentration of MSN-LIPO intratumorally and intravenously, respectively. The mice in the two groups were scanned by MRI before and after injection, and the image characteristics were studied and the signal intensity of tumors was statistically analyzed. Results In vitro MRI imaging showed that the signal intensities of the images were gradually decreased with the increase of iron concentrations in MSN-LIPO, and 1/T₂ had a good linear relationship with the concentration within a certain range, with the relaxation rate (r₂) being 413.7 mmol^-1·s^-1. MRI imaging of BALB/c nude mouse models with malignant glioma showed that the signal intensity of the tumor after intra-tumor injection of MSN-LIPO was significantly lower than that before intra-tumor injection (15.34±1.24 vs 211.44±5.34, t=36.38, P<0.05), and the signal intensity of the tumor after intravenous injection of MSN-LIPO was significantly lower than that before intravenous injection (179.00±4.35 vs 235.99±5.17, t=14.34, P<0.05). Conclusion MSN-LIPO has good magnetic relaxation property and good MRI T₂ weighted contrast enhancement function in vivo, suggesting that it can be used as a MRI T₂ contrast agent.