Objective To investigate the relationship between resilience, cognitive emotion regulation strategies and fatigue reaction of navy soldiers. Methods A total of 846 soldiers were selected from a navy troop by convenience sampling method, and were tested by cognitive emotion regulation questionnaire-Chinese version (CERQ-C), Connor-Davidson resilience scale (CD-RISC) and multidimensional fatigue inventory scale (MFI-20). SPSS 21.0 software were used to analyze the relationship between the variables. Results Non-adaptive emotion regulation strategy was positively correlated with fatigue reaction in the soldiers (P<0.01), and the three dimensions of resilience were negatively correlated with fatigue reaction in the soldiers (P<0.01). Both resilience and cognitive emotion regulation strategies could independently explain the variance of fatigue reaction of the soldiers, in which self-improvement (β=-0.206, P<0.05) and adaptive strategies (β=-0.229, P<0.01) had significant negative predictive effects on fatigue reaction of soldiers, while non-adaptive strategies (β=0.406, P<0.01)had significant positive predictive effects on fatigue reaction. The mediating effective value of resilience on adaptive strategies and fatigue reaction of soldiers was 0.53×(-0.36)=-0.19, z=7.02, and on non-adaptive strategies and fatigue reaction of soldiers was (-0.30)×(-0.36)=0.108, z=5.12. Conclusion Cognitive emotion regulation strategies has direct effect on fatigue reaction of navy soldiers, and it also has indirect influence on the fatigue reaction through the mediating effect of resilience.