Objective To evaluate the anti-G effect of an anti-G straining maneuver (AGSM) for flight instructors proposed earlier by our research group using surface electromyography (sEMG). Methods Eight subjects aged 18-20 years were enrolled. The test equipment was AMST-HC-4E manned centrifuge. According to the performance and load characteristics of primary and advanced trainers, 5 centrifuge acceleration curves of 3.0G60s, 4.0G50s, 5.0G40s, 6.0G30s and 7.0G20s were compiled to evaluate the anti-G effect of FT maneuver and HP maneuver, respectively. At the same time, sEMG was used to observe the changes of muscle strength and muscle activation of 2 AGSMs before and after training under different loads. Results After training, the G tolerance of subjects using FT maneuver and HP maneuver was significantly higher than that before training. Compared with before training, the G tolerance of subjects in FT maneuver group increased by 60.8%, HP maneuver group increased by 34.6%, FT maneuver+KH-X anti-G suit group increased by 50.5%, and HP maneuver+KH-X anti-G suit group increased by 51.7% (all P<0.05). The results of sEMG test showed that muscle integrated electromyography (iEMG) of subjects using FT maneuver and HP maneuver all showed a downward trend after training. Significant differences were found in the decrease of iEMG values of the right gastrocnemius muscle under 2.5G10s load, left and right rectus abdominis muscle and right gastrocnemius muscle under 3.0G60s load, and left rectus abdominis muscle under 5.0G40s load+KH-X anti-G suit before and after training (all P<0.05). Compared with HP maneuver, left anterior tibial muscle iEMG of subjects using FT maneuver under 3.0G60s load was significantly higher (P< 0.05). The left rectus abdominis muscle iEMG of subjects using HP maneuver was significantly higher than that of subjects using FT maneuver in 4.0G50s load+KH-X anti-G suit and 5.0G40s load+KH-X anti-G suit groups (both P<0.05). Conclusion sEMG assessment has showed that the anti-G effect of FT maneuver proposed for flight instructors is good.