Objective To explore the characteristics of response inhibition of military university students during multitasking operation. Methods Repeated measures of ANOVA as well as distribution test were employed to explore how the performance of 127 military university students in Go/No-Go test was affected by simulated driving task. Results The test results of 127 participants showed that there was a interaction between the interference task and the Go trial proportion on the hit rate and false alarm rate, that is, no significant difference was observed between the 60% and 40% of trial proportion without interference task (both P＞0.05), but the hit rate and false alarm rate in the 60% trial proportion condition were significantly higher than those in the 40% trial proportion condition under interference task (both P＜0.01). In addition, significant main effects of interference task were observed on hit rate, false alarm rate, and discrimination index d’ (all P＜0.01), that is, the interference task reduced the hit rate and discrimination, but increased the false alarm rate. Moreover, individual differences existed in the discrimination index d’ changes, and the participants were divided into easily disturbed group (n＝23, 18.11%), undisturbed group (n＝20, 15.75%), and intermediate group (n＝84, 66.14%) by adding or subtracting 1 standard deviation from the mean of the difference. Conclusion The interference tasks increase the psychological load of military university students during multitasking operation, and impair the response inhibition; and individual differences exist in response inhibition.