Objective To reduce the mechanical impact caused by postoperative creep and relaxation of tendon grafts by pre-setting high-strength sutures using suture weaving. Methods Fresh pig tendo calcaneus specimens were used to prepare 30 anterior cruciate ligament tendon grafts, and they were randomly divided into control group, 2-strand suture implantation group, and 4-strand suture implantation group, with 10 tendon grafts in each group.The control group were not given suture implantation in the tendon grafts, and the 2- and 4-strand suture implantation groups were pre-set 2 and 4 strands of high-strength No.2 sutures, respectively.The tendon grafts in each group were subjected to 1, 100, 500, 1 000, 1 500, 2 000, 2 500, and 3 000 fatigue tests in turn, and the relaxation was recorded.Finally, the tendon grafts in each group were pulled to failure at a constant rate of 5 cm/min, and the traction force when the relaxation reached 5 mm, i.e.the failure load, was recorded. Results The relaxation of tendon grafts after each fatigue test was significantly lower in the 2- and 4-strand suture implantation groups than that in the control group, and the relaxation was significantly lower in the 4-strand suture implantation group than that in the 2-strand suture implantation group (all P＜0.05).The failure loads of tendon grafts were significantly higher in the 2- and 4-strand suture implantation groups than that in the control group, and the failure load was significantly higher in the 4-strand suture implantation group than that in the 2-strand suture implantation group (all P＜0.05). Conclusion Pre-setting high-strength sutures in tendon grafts by suture weaving can effectively improve the mechanical properties of tendon grafts.