Objective To investigate the protective effects of Wuling capsule on mice with autoimmune hepatitis (AIH). Methods Mice were randomly divided into control group, AIH model group, Wuling capsule low-dose group (0.5 g·kg^－1·d^－1), Wuling capsule middle-dose group (1.0 g·kg^－1·d^－1), and Wuling capsule high-dose group (2.0 g·kg^－1·d^－1), with 10 mice in each group. The Wuling capsule groups were administered with Wuling capsule suspension of different doses orally at a volume of 10 mL/kg once daily; the control group and AIH model group were given the same volume of saline by gavage. After 14 d of administration, mice in the AIH model group and Wuling capsule groups were injected with concanavalin A (20 mg/kg) via the tail vein, and the serum, liver, and spleen were collected 8 h after injection. Serum alanine transaminase (ALT) and aspartate transaminase (AST) levels were measured using an automatic biochemical analyzer; hematoxylin-eosin staining was used to observe the pathological structure of the liver tissue; the contents of interleukin (IL)-6, IL-4, and tumor necrosis factor (TNF)-α in liver were determined by enzyme-linked immunosorbent assay, and quantitative polymerase chain reaction was used to determine the relative mRNA expression levels of IL-6, IL-4, TNF-α, Toll-like receptor 4 (TLR4), and nuclear factor κB (NF-κB) in the liver. Fluorescence immunoassay was used to analyze the expression and nuclear translocation of NF-κB p65 protein in the liver. Results Compared with the control group, the AIH model group showed abnormal liver morphology and structure, increased serum ALT and AST levels, increased contents of IL-4, IL-6, and TNF-α in the liver, upregulated mRNA expression levels of IL-6, IL-4, TNF-α, TLR4, and NF-κB in the liver, and increased nuclear entry of NF-κB p65. Wuling capsule significantly improved the pathological structure of the liver in AIH mice, reduced serum ALT and AST levels, decreased the contents of IL-4, IL-6, and TNF-α in the liver and the mRNA expression levels of TLR4, NF-κB, IL-4, IL-6, and inhibited the nuclear activation of NF-κB p65. Conclusion Wuling capsule has significant protective effects on AIH mice, which may be related to the TLR4/NF-κB signaling pathway.