Wound healing is a complex and highly coordinated physiological process, involving the interaction of various cell types, growth factors, and extracellular matrix. Its goal is to repair damaged tissue and restore skin integrity and function. However, various internal and external factors can lead to delayed or abnormal wound healing, causing significant pain and economic burdens for patients. Therefore, it is of great clinical significance to develop safe and effective treatments to promote wound healing. In recent years, research on natural products in the field of wound healing has attracted widespread attention, and berberine, a biological alkaloid with multiple pharmacological activities, has garnered considerable attention for its remarkable effects in promoting wound healing. This article provides an in-depth review on the progress in the application of berberine in wound healing from multiple perspectives.