Objective: To study the effect of anti-CD3-anti-glioma bispecific antibody (BsAb) on human glioma growth in nude mice. Methods: There were 6 groups in these experiments, basic group was treated with BsAb, others were different control groups. Nude mice-human glioma model NHG-1 was used to observe the occurence, growth of the tumor and survival periods. Results: In the basic group, the average incubation period, average tumor cells cycle, and average survival periods were postponed compared with all control groups (P<0.05 or P<0.01). According to tumor growth trend, different control groups went into rapid growth at different time after several week′s slow growth. However, the basic group treated with BsAb lived in a slow growth rate in the whole survival. Conclusion: BsAb can decrease the growth rate of tumor in nude mice, and it may has potential usage in clinic.