Objective: To determine the HGV infection rate and status of sup er-infection with other hepatitis virus in hemodialysis patients. Metho ds: Fifty seven serum samples were collected from hemodialysis patients. T wo sets of primers were designed and synthesized according to the conserved regi on at 5′ non-coding region of HGV RNA sequence. HGV RNA fragment was amplified by reverse transcript-nested PCR, and IgG antibody to HGV was tested by EIA. M eanwhile, HBV DNA and HCV IgG antibody were tested in all samples. Results : Among 57 serum samples detected, 4 samples were positive for HGV RNA(7.0%) , and 2 were positive for HGV IgG antibody (3.5%).Among samples positive for HGV RNA, 2 were positive for HBV DNA, and 2 were positive for HCV IgG antibody, and one of which was positive for both HBV and HCV markers. Those samples positive for HGV RNA were negative for HGV IgG antibody and vice versa. Concl usion: The infection rate of HGV in hemodialysis patients may be higher th an i n healthy blood donors and healthy population. Patients with HGV infection can b e super-infected with HBV and HCV.