Objective:To investigate the effect of TNFα on insulin sensitivity and some relative states in normal rats. Methods: The changes of K value in glucose-insulin tolerance test,the concentration of glucagon and ACTH,the lipids in serum and the contents of glycogen,triglyceride in tissues were tested in normal rats after low dose injection of TNFα. Results: The K values in glucose-insulin tolerance test decreased after TNFα injected ,the concentrations of ACTH,GC and serum lipids all increased,and the glycogen contents in liver and red quadriceps decreased,the liver TG depots increased. Conclusion: TNFα can induce evident insulin resistance in normal rats, the improvement of plasma insulin antagonistic hormone level and some disorders in sugar or lipid metabolism may be involved in.