Objective: To prepare the monoclonal antibodies against ciliary neurotrophic factor(CNTF). Methods: Cell lines of hybrids secreting monoclonal antibodies against CNTF were established by fusing SP2/0 myeloma cells with spleen cells of BALB/c mice immunized with CNTF and cloning 3 times; asitic fluid and serum containing abundant monoclonal antibodies against CNTF were obtained by in vivo induction; then identification of the monoclonal antibodies against CNTF and detection of antibody titer were undergone. Results: Five cell lines of hybrids secreting monoclonal antibodies against CNTF were established. Subtypes of these antibodies were all IgG2a. Antibody titers in asitic fluid and serum were 1∶107-1∶108 and 1∶106, respectively. These monoclonal antibodies showed specificity in demonstrating neurons secreting CNTF in CNS, and one kind of them inhibited the enhancing effect of CNTF on the proliferation of neonal SD rats skeletal muscle cells in culture. Conclusion: The monoclonal antibodies against CNTF with high activity and specificity have been established successfully, and will be a useful tool in the studies of CNTF"s structure,function and mechanism