Objective: To use analogtodigital conversion technique to mensurate the time interval in skeletal muscle during excitation-contraction coupling. Methods: Two way infrared detectors, analogtodigital conversion card, Rechert Jung KF-80 quick freezing fixation were employed in this test. Results: The time internal of 0.8,5.6,8.4 ms during the skeletal muscle function change was measured, and the morphological images were synchronously obtained. Conclusion: Analogtodigital conversion technique provides the methodology for the function-morphological study