Objective:：To increase tumor immunogenicity by enhancing gene expression of TNF,IFN,MHC Ⅰ molecule , adenoviral vectors containing HuTNFα and IFNβ cDNA driven by HLA-B7 inducible promoter and CMV promoter were constructed, respectively. Methods and Results：The 2 fragments HLA-B7 promoter-TNF and IRES-IFN were isolated from and pGL3B7TNF and pIRIF and insert to pBluescript Ⅱ Sk (+) to generate pBLB7TNIRIF.TNFα-IRES-IFNβ was isolated and ligated to AdCMVLink1 to generate AdCMVTNIRIF(+), in which gene transcription was driven by CMV promoter.B7pro-TNFα-IRES-IFNβ was isolated and ligated to AdBglⅡto generate AdB7TNIRIF(-),in which gene expression was driven by HLA-B7 promoter. Conclusion：The constructed vectors can be a new route for enlarging tumor immunogenicity.