Abstract:Objective：To transfuse rhesus bone marrow derived mesenchymal stem cells （MSCs） to rhesus leucocytes antigen （RhLA） mismatched recipients, in attempt to study the immunological reaction between recipients＇ T lymphocytes and the skin of donor or a third party. Methods： Bone marrow aspirates were collected from femurs of donor rhesus, and the mononuclear cells were isolated and resuspended in low glucose Dulbecco＇s Modified Eagles Medium （DMEM） containing 2% fetal bovine serum. Phenotype of MSCs was analyzed by flow cytometry and the differentiated cells were identified by relevant specific staining. Allogeneic MSCs were given to recipients （single infusion） without preconditioning. Peripheral blood lymphocytes from recipients were co-cultured in vitro with the donor＇s skin or a third rhesus for 72 h before and 20 days, 40 days, and 60 days after infusion. The pathological lesions were evaluated and the keratinocyte apoptosis of the skin sections was determined by TUNEL assay. Results： Twenty days after infusion, the pathological lesion in in vitro skin explants from MSCs donor was less and the apoptosis rate was declined to （12.2± 1.9）% from a pre-infusion level of （23.0±4.9）%. The apoptosis of cells in skin explants from the third party also declined to （11.8±1.4）% from a pre-infusion level of （22.0±2.0）% （P〈0.05）. Intensive immunological reaction was observed between recipient and donor/the third party on day 40 and day 60 after transplantation, with the apoptosis rate being （22.0±3.2）%, （22.0±2.5）% （donor） and （23.2±2.4）%, （24.0±2.6）% （the third party）. Conclusion： Allogenic MSCs transfusion can decrease the immunological reaction between donors and recipients in rhesus, but this decreased reaction is temporary and reversible.

Abstract:Objective.-To investigate the dynamic expression of chemokines Mig, IP10, and ITAC after liver transplantation and to study its role in early diagnosis of acute rejection in humans. Methods： Thirty patients receiving liver transplantation （April 2005 to September 2005） were divided into acute rejection （AR） group （n=9） and non-acute rejection （NAR） group （n= 18） based on the clinical symptoms and pathological examination. Three patients were excluded due to post-operation infection. The ehemokines expression was determined in all patients 1 day before and day 1, 3, 5, 7 after transplantation. Sixteen patients with liver cirrhosis （cancer） and 16 normal adults were also examined as control in this study. Patients in AR group received pulse glueoeortieoid treatment from the onset of AR and the expression of chemokines was determined on the day of AR diagnosis and day 3, 7 after glueocortieoid treatment. The relationship between Banff rejection activity index （RAI） and 3 ehemokines expression on the day of AR onset was analyzed. Results： Chemokines expression was not significantly different between transplantation group and liver cirrhosis （cancer） group one day before transplantation; however, it was significantly higher than that of the normal control group （P〈0. 01）. The expression of Mig, IP10 and ITAC was increased markedly in AR and NAR group day 3 after transplantation （P〈0.05）. AR was confirmed in 9 patients on day 11, 12 and 14 after transplantation. The serum contents of Mig, IP10 and ITAC in AR group were higher than those in NAR group at all defined time points after transplantation. There was a positive relationship between RAI and the expression of Mig, IP10 and ITAC on the day of AR onset （r=0. 88, 0.94, 0. 80, respectively）. In AR patients, the expression of Mig, IP10 and ITAC decreased after pulse treatment with glucocorticoid （P〈0.01）. Conclusion： Serum level of Mig, IP10 and ITAC can serve as a sensitive, specific marker for early predication of AR in liver transplant patients.

Abstract:Objective：To investigate the expression of P-glycoprotein （P-gp）, multidrug resistance-related protein （MRP）, lung resistance-related protein （LRP）, P53 and c-erbB-2 in untreated non-small cell lung cancer （NSCLC） and their relationship with each other. Methods： The expression of P-gp, MRP, LRP, P53 and c-erbB-2 was detected in the carcinoma tissues of 78 NSCLC patients and 15 adjacent normal lung tissues （5 cm apart from tumors） by means of immunohistochemistry. Results： The positive rates of P-gp, MRP, LRP, P53 and c-erbB-2 in tumor tissues were 65.4 %, 39.7 %, 56.4 %, 53.8 % and 43.6 %, respectively, all significantly higher than those in normal tissues（P〈0.05）. There was also significant difference in MRP and LRP expression between different pathologic types of NSCLC（squamous cell carcinoma, adenocarcinoma, large cell carcinoma） （P= 0. 008 and G0. 001, respectively）. Expression of LRP and P53 was also significantly different between different degrees of pathological differentiation （well, moderate, poor） （P=0. 025 and 0. 026, respectively）. The expression of c-erbB-2 was correlated with those of P-gp, MRP, LRP and P53 （Spearman R=0. 317, 0. 401, 0. 519 and 0. 341, respectively;P=0. 005, G 0. 001, G0.001 and =0.002, respectively） ; the expression of P53 was correlated with that of MRP （Spearman R=0. 260,P= 0. 022） ; and the expression of LRP was correlated with that of MRP （Spearman R = 0. 371, P= 0. 001 ）. Conelusion： Overexpression of P-gp, MRP, LRP, P53 and c-erbB-2 may play a cooperative role in the drug resistance and development of NSCLC.

Abstract:Objective：To investigate the morphology and growth kinetics of the cells derived from the adult normal and herniated nucleus pulposus tissues. Methods： The cells derived from the adult normal and herniated nucleus pulposus were cultured in vitro. Passage 1,3,5 cells were chosen for light microscope and electron microscope observation. The cell growth rate, MTT value, maximal growth density, cellular mitotic index, cloning efficiency, and other parameters were observed in normal and herniated nucleus pulposus and the results were compared. Results： Compared with adult normal nucleus pulposus cells, the cells isolated from herniated nucleus pulposus were irregularly arranged and lost polarization, with rough endoplasmic reticulum slightly dilated. Under the same cellular density and culture conditions, the growth rate of passage 1 cells from adult normal and herinated nucleus pulposus had no significant difference, but the differences of passage 3 cells（P〈0.01） and passage 5 cells （P〈0.05） were statistically significant. The maximal growth density, MTT value, cellular mitotic index, and cloning efficiency of passage 1 cells of adult normal and herinated nucleus pulposus were respectively 7.28 ±0.23 and 7.41 ± 0. 17,0. 186 ± 0. 015 and 0. 173±0. 008, （25.36±2. 14）‰ and （23. 13±2.55）‰,and （52.4±2.8）% and （47.7±2.6）% （all P〉0.05）; those of passage 3 cells were respectively 6.96 ± 0.19 and 5.48± 0.22, 0.154 ± 0.018 and 0.110 ± 0. 009, （ 22.52 ± 2.16） ‰ and （16.68±2.35）‰, and （48. 7±3.3）% and （40.7±2.5）±;and of passage 5 cells were respectively 2.42±0. 13 and 0.98± 0.14, 0.085±0. 006 and 0. 050±0. 007, （15.61± 1.96）%o and （9.34±2. 12）%0, and （40.2±2.3） % and （28.34± 2.9） %. There were significant differences between the parameters of passage 3 and 5 cells（P〈0.05）. Conclusion： The adult normal and herinated nucleus pulposus cells are different in cellular morphology when cultured in vitro. The continuous proliferation ability of the herniated nucleus pulposus cells is obviously decreased than that of the adult normal control group,and aging is more easi- ly seen in the herniated ones

Abstract:Objective： To study the influence of occlusal force change on apoptosis of periodontal ligament fibroblast （PDLF） and expression of proliferating nuclear antigen （PCNA） in the fibroblasts, so as to explore the possible mechanism for remodeling of periodontal ligament. Methods： Animal model of occlusal force change was established by extracting the right first, second and third maxillary molars in male SD rats. The rats were sacrificed at 6, 12 hours and day 1, 2, 3, 5, 7, 14, and 28 d after extracting the teeth（n = 6）, and their alveolar bone tissues were harvested. H-E staining was used to observe the morphologic changes of the alveolar bone tissues. TUNEL method and immunohistochemistry were used to detect apoptosis and PCNA expression in PDLF. The rats with normal occlusal forces were used as control（n= 6）. The results were assigned a mean score based on the percentage and the intensity of cells positively stained for TUNEL and PCNA. Results：Animal model of occlusal force change was successfully established, H-E staining showed that the control group had more pycnotic periodontal ligament, orderly fibres, and flatter alveolar bone than the model group. TUNEL result showed that the cell apoptosis reached its peak at 12 haffer extracting the teeth （252.67 ± 6.62）, significantly higher than that of control group （7.17 ± 2.32, P〈0.01 ）, then gradually decreased to normal level on day 28 after extraction （8. 50 ± 1.87）. Immunohistochemical staining showed that the expression of PCNA had a similar tendency of apoptosis：PCNA expression peaked on day 3 after extraction （246.00 ± 7.21） and then gradually decreased to normal level （5.67 ± 2.16, P〈0.01）. Conclusion： Changes of occlusal force can facilitate the PDLF apoptosis and promote PDLF proliferation, contributing to the remodeling of periodontal ligament

Abstract:Objective：To compare the root filling results of 2 thermoplasticized gutta-percha filling techniques （Ultrafil 3D and Obtura Ⅱ ） with cold lateral condensation of gutta-percha. Methods： Sixty freshly extracted maxillary mandibular single- rooted teeth were instrumented and randomly divided into 3 groups： Ultrafil 3D, Obtura Ⅱ and cold lateral condensation groups. The root canal was routinely prepared and the obturation time was recorded. Three days later, 10 teeth of each group were ran- domly stained and the micro effusion length was subsequently measured. The micro-chink between filling materials and the wall of root canal were measured with scanning electron microscope in the other 10 teeth of each group. The data were analyzed using unpaired Student＇s t-test. Results： The longest obturation time was in cold lateral condensation group, then Obtura Ⅱ and Ultrafil 3D group in order, with significant difference found between the cold lateral condensation group and the latter 2 groups （P〈0.01）. The lengths of micro effusion and micro-chink in lateral condensation group were significantly larger than those in the other 2 groups （P〈0.05） ; however, there was no significant difference between the 2 injection thermoplasticized gutta-percha technique groups. Conclusion： Under in vitro condition, the 2 injection thermoplasticized gutta-percha techniques have better adaptation to the canal wall, better root filling results, and less obturation time compared with cold lateral condensation

Abstract:Objective：To study the differentially expressed genes associated with lipid metabolism in adipose tissues of patients with insulin resistant （IR） type 2 diabetes mellitus（T2DM）, in an effort to explore the mechanism of IR. Methods： mRNA from the omental adipose tissues of T2DM patients （n=5） and normal controls （n=5） were reversely transcribed into cDNAs with the incorporation of fluorescent dUTP （cy-5 or cy-3） to prepare hybridization probes. The mixed probes were hybridized with a cDNA microarray containing the target genes. The results were scanned and subjected to computer analysis to search for the difference between the gene spectrum of T2DM patients and normal controls. The differentially expressed gene, FOXC2, was verified by Northern blot. Results： Eighty-two differentially expressed genes were identified between these 2 groups, with 10 associated with lipid metabolism, including 5 upregulated ones and 5 downregulated ones. Northern blotting confirmed that the expression of FOXC2 mRNA was increased in IR group, which was in accordance with the result of cDNA microarray analysis. Conclusion： The pathogenesis of IR is related with lipid metabolism and the FOXC2 gene may be a candidate gene of IR

Abstract:Objective：To study the interaction between TrkA and downstream of kinases 1（dok 1） and search for the possible intracellular substrates or regulatory proteins of TrkA, so as to better understand the mechanism of downstream signal transduction of TrkA. Methods： The TrkA^IC （intracellular domain of TrkA） was fused with LexA and the product was used as a DNA-binding domain protein; dok1, dok1PTB and dok1△PTB（dok1 without PTB） were separately fused with B42AD and their products were used as activation domain protein. Cotransformants were subjected to β-galactosidase activity analysis and leucine medium growth analysis for understanding of their interaction. Results： Dokl and dok1PTB interacted with TrkA in yeast, while dok1△PTB did not. Conclusion： It is confirmed that TrkA can interact with dok1 in yeast, and the interaction may be mediated by PTB domain of dok1.

Abstract:Objective：To study the changes of hepatic caspase-3 and caspase-8 activities during liver regeneration in rats. Methods： Liver regeneration model was produced by 70% partial hepatectomy （PH） in male SD rats. Then the rats were sacrificed and the liver homogenate were prepared. Hepatic caspase-3 and caspase-8 activities were determined at 8 different time points during the liver regeneration at the absorbance of 405 nm. Results： Hepatic caspase-3 activity increased significantly 3 h, 6,72 h, 120 h and 168 h after PH compared with that of control group（P〈0. 001）; Hepatic caspase-8 activity increased 168 h after PH（P%0.05） and had no obvious change at any other time points. Conclusion： Our result suggests that the increase of caspase-3 activity during the liver regeneration may be mainly induced by the mitochondrial pathway.

Abstract:Objective：To investigate the expression and correlation of cyclooxygenase-2 （COX-2） and c-erbB-2 in human breast cancer. Methods： The expressions of COX-2 and c-erbB-2 in 48 breast cancer patients with breast cancer were detected with immunohistochemistry examination and was analyzed. The relationship between their expression and the clinicopathologic characters of breast cancer was also analyzed. Results： The positive rate of COX-2 was 60.4% （29/48） in these patients; the expression was higher in patients with tumor size larger than 2 cm, lymph node metastasis, and negative expression of ER or PR, but was not related with the ages, the pathologic types and the TNM stages. Expression of c-erbB-2 was identified in 39.6% （19/48） of these patients; the expression was significantly higher in patients with lymph node metastasis or negative expression of ER and PR, but was not related width the ages, the sizes and the pathologic types. COX-2 was positive in 84.2% （16/19） of patients positive of c-erbB-2 and in 44. 8 % （13/29） of patients negative of c-erbB-2, with significant difference found between the 2 figures（P=0. 006）. Conclusion： The high expression of COX-2 in breast cancer is related to the expression of c- erbB-2, suggesting that they may regulate each other and jointly contribute to the tumorigenesis and progression of breast cancer.

Abstract:Objective.. To investigate the effect of co-exposure to LPS and heat on plasma malondialdehyde （MDA） content and superoxide dismutase （SOD） activity in rats. Methods： Male specific pathogen-free Wistar rats were randomly assigned to the following groups., saline injection＋normothermic control （C-Group）, saline injection＋heat exposure （H-Group）, LPS injection＋normothermic control （L-Group）, and LPS injection＋heat exposure （HL-Group）. Rats in H-/HL-Group were exposed in a chamber at an ambient dry bulb temperature （Tdb） of （35.0±0. 5）℃ and in C-/L-Group at an ambient Tdb of （26.0＋0.5） ℃. Rats in L-/HL-Group were given an intravenous injection of LPS 10 mg/kg via tail veins to induce endotoxemia and in C-/H-Group were given an intravenous injection of 0. 9% NaCl （10 ml/kg） via the tail vein. Mean arterial pressure （MAP） was continually monitored in all rats. Plasma levels of MDA and SOD activity were determined at 0, 40, 80, 120 rain after exposure. Results： There was significant difference in plasma MDA levels and activities of SOD between L-/H-/HL-Group and C-Group （P〈0.05）. The rats in HL-Group displayed significantly increased MDA level and decreased SOD activity compared with those in the other 3 groups（P〈0.05）. Conelusion. This study suggests that co-exposure to LPS and heat can promote and augment systemic inflammatory response syndrome in rats.

Abstract:Objeetive：To investigate the therapeutic effect of oat β-glucans on serum lipid levels of rats with experimental hyperlipemia. Methods： Rats were fed on high fat forage for 3 weeks to establish hyperlipemia models, then the model rats were orally given oat β-glucans at doses of 133, 266 and 533 mg/（kg · d） separately. At the same time, there were 3 control groups including chitosan ligosaccharide[200 mg/（kg · d）], normal, and high blood lipid （model rats fed with distilled water）. Serum levels of total cholesterol （TC）, high density lipoprotein cholesterol （HDL-C） and low density lipoprotein cholesterol （LDL-C） were assayed in rats of all groups and the results were compared. Results.. β-glucans obviously decreased the levels of TC and LDL-C and increased HDL-C in rat hyperlipemia models. The reduction of serum lipid level in 4-week β-glucans treatment rats was more obvious than that in 3-week β-glucans treatment rats. Besides, β-glucans showed an obvious dosage dependent effect in reducing serum lipid level without adverse effect. Oat β-glucans had a better lipid-reducing effect than ligosaccharide did. Conclusion.. Oat β-glucans can obviously decrease serum lipid in rats with hyperlipemia.

Abstract:Objective：To provide anatomic data for accurately localizing aperture of sphenoidal sinus without injurying sphenopalatine arteries in endoscopic transsphenoidal sella surgery. Methods： The anterior walls of spheroid sinus were observed and measured （layout, position, external diameter,distances to other structures of interest） with a vernier caliper and an angle gauge on 15 adult cadavers. Results： The distances from the root of columella nasi to the inforior pole of sphenoidal sinus aperture and the sphenopalatine foramen were （60.40±3.21,58.10-72.76） mm and （62.14±1.93,59.50-73.40） mm, respectively. The distance from the inforior pole sphenoidal sinus aperture to the sphenopalatine foramen was （12.20±1.10,8. 10-16.35） mm. The diameter of the sphenopalatine artery was （1.99 ±0.13,1. 50-2. 80） mm. The distances from the inforior pole of sphenoidal sinus aperture to the superior and inferior posterior artery of nasal septum were （3.49±0. 24,2. 78-5.20） mm and （6.42±1, 08, 4.30-8. 50） mm, respectively . Conclusion： Aperture of sphenoidal sinus is an important marker in endosopic transsphenoidal sella surgery. The anterior wall of sphenoid sinus should be opened from the inforior pole of sphenoidal sinus aperture to avoid injurying sphenopalatine artery.

Abstract:Objective：To prepare levofloxacin-carboxymethyl-chitosan （LVFX/CMC） microspheres and to study their colon-targeted release in rats. Methods： KromasilRKR100-5C18（250 mm× 4.6 mm）was used as the analytical column and the temperature was maintained at 50℃. The mobile phase consisted of a mixture of acetonitrile： 0. 1 % trifluoroacetic acid （20 ： 80） pumped at a flow rate of 1.0 ml/min, with λex 295 nm and hem 495 nm. Sixty healthy male SD rats were randomized into 2 groups： to receive gastric lavage with normal LVFX （40 mg,control group） and LVFX/CMC microspheres （40 mg,treatment group）. HPLC was used to detect the concentrations of LVFX in the stomach,intestine,cecum,colon and blood in rats in both groups. Results： LVFX was detectable 3 h after LVFX/CMC microsphere lavage. Five hours after lavage,the levels of LVFX in cecum and colon were respectively （3. 394±0. 197） mg and （1. 873±0. 216） mg in treatment group,and （0. 489±0. 123） mg and （0. 078±0. 002） mg in control group （P〈0. 01） . Nine hours after lavage,the levels of LVFX in cecum and colon were （1. 580±0. 234） mg and （0. 713±0. 073） mg in treatment group,and （0. 105±0. 023） mg and （0. 054±0. 016） mg in control group（P〈0.01） ,respectively. Serum level of LVFX peaked at 5 h in treatment group and at 1 h in control group. The concentrations of LVFX were obviously higher in cecum and colon than those in stomach and intestine. Conclusion： The prepared LVFX/CMC microspheres have the property of colon-targeted release in rats.

Abstract:Objective：To study the long-term （iv for 30 days） toxic effect of recombinant batroxobin （rBAT） on Macaca rnulatta. Methods： Twenty-four Macaca mulatta （12 male and 12 female） were randomly divided into 4 groups according to their body weights （n= 6） ： rBAT treatment groups （1.5, 5.0, 15.0 kU/kg of rBAT once daily for 30 consecutive days through iv injection） and acetate buffer treatment group （as control）. Half of the animals in each group were sacrificed at the end of medication and the rest were sacrificed after another 15 days＇ recovery. The general symptoms, the hematological, chemical parameters, urinalysis values, ECG results, bone marrow findings, pathologic changes and the antibody detection were observed or performed in all groups. Results： On d15 and d30, the blood samples of each group were collected at 30 min and 24 h after iv injection. Compared with control group or with dO data of each corresponding group, the fibrinogen （Fib） was significantly decreased 30 min after iv injection of rBAT, and the decrease was in a dose-dependent manner. Fib returned to the normal level 24 h after iv injection. Histopathological examination showed congestion and slight edema of liver and kidney cells in each group, probably due to latent infection of the animals. On d30, there was local inflammatory reaction in the injection sites of some animals in each group, but none was found on d45. Non-neutralizing antibodies to rBAT were continuously detected from d15 to d45. Conclusion.. rBAT has pharmacological and toxicological effects on hematology system of Macaca mulatta. It can dose-dependently decrease Fib. The target organ of rBAT is the hematology system and the effect of rBAT is reversible. The safety dose of rBAT for Macaca mulatta is 1.5 kU/kg. Attention should be given when rBAT is used clinically.

Abstract:Objective：To compare the anti-aging effects of ginsenosides,tanshinone and chuanxionyzine on the skin of D-galactose-induced mice aging model. Methods.. One hundred female mice were randomly d[vided into 5 groups（n= 20）. Mice in normal control group received subcutaneous injection of normal saline and mice in other 4 groups received cervicodorsal injection of D-galactose （1 000 mg·kg^-1·d^-1 ） for 42 d to establish subacute aging model. The experimental groups included aging model group （without further treatment）, tanshinone-treated （1 500 mg·kg^-1·d^-1） group,chuanxionyzine-treated （150 mg·kg^-1·d^-1） group,and ginsenosides-treated （100 mg·kg^-1·d^-1） group. Forty two days later,the dorsal skin samples were collected to determine SOD,CAT activities and MDA,hydroxyproline contents. Results： In tanshinone- and ginsenosides-treated groups,SOD activities increased from （131.2±21.5） U/ml to （203.1 ±11.2） U/ml and （196.8±27.5） U/ml,respectively; the contents of hydroxyprolin increased from （0.57±0.13） mg/g to （0.71±0.11） mg/g and （0.76±0.12） mg/g,respectively; and the contents of MDA decreased from （9.39± 1.5） nmol/g to （6.4±1.3） nmol/g and （7.16 ± 2.3） nmol/g, respectively （all P〈0.05）. However, there was no significant difference between the parameters of tanshinone- and ginsenosides-treated groups. Compared with aging control group, there was no obvious changes in the above parameters in chuanxionyzine-treated group. Conclusion： Both ginsenosides [100 mg/（kg ·d） ]and tanshinone [1 500 mg/（kg ± d）] have noticeable anti-skin aging effects via i.g. administration through improving the activities of antioxidant enzyme and dermal fibroblasts. Chuanxionyzine [150 mg/（kg · d）] shows no anti-skin aging effects in mouse aging models.

Abstract:Objective：To determine the changes of mangiferin and neomangiferin contents in Rhizoma Anemarrhenae before and after processing. Methods： High performance liquid chromatography （HPLC） was used to determine the changes of mangiferin and neomangiferin contents in Rhizoma Anemarrhenae before and after processing. The mobile phase was ACN： 25 mmol/L KH2 PO4 （pH 3.0）, gradient elution. The Similarity Evaluation System for Chromatographic Fingerprint of TCM （2004 A edition） was used to evaluate the similarity of the 3 batches of Rhizoma Anemarrhenae before and after processing. Results： Mangiferin in the 3 batches was upregulated and neomangiferin was downregulated afer prosessing. The similarity of the 3 batches was all decreased（〈 1）. Conclusion： Processing changes the contents of mangiferin and neomangiferin in Rhizoma Anernarrhenae.

Abstract:Objective：To assess the efficacy and tolerability of sibutramine as a weight reduction agent in treatment of overweight patients with type 2 diebetes. Methods： Forty overweight patients （body mass index ≥25 kg/m^2） with type 2 diebetes were randomized into sibutramine treatment group and control group（n= 20）. Both groups received a reduced-calorie diet （deficit of 1 045-2 090 kJ/d from an average daily intake） and continued their original treatment; sibutramine treatment group was also given sibutramine 15 mg once daily for 12 weeks. Before and after sibutramine treatment, body mass index （BMI）, fasting blood glucose （FBG）, fasting serum insulin （FINS）, hemoglobin Alc（ HbAlc ）, and serum lipids were measured in the patients. Homeostasis model assessment （HOMA） was applied to assess the status of insulin resistance （IR）. Results： After treatment, BMI, FBG, H bAlc, TG, FINS and H OMA-IR all decreased （P〈0.01） in sibutramine treatment group. Also in sibutramine treatment group the changes in FBG and TG were correlated with percentage change in body weight （P〈0.01）. Four patients in sibutramine treatment group had a ≥5% weight loss and great decreases in HbAlc and TG. Mild adverse events were no- ticed. Control group had no significant difference in every measurement before and after treatment. Conclusion： Sibutramine can produce significant weight loss in overweight type 2 diabetes patients when used with a reduced-calorie diet; this weight loss is associated with improvement of metabolic control and insulin sensibility. Meanwhile, sibutramine is well tolerated and has less adverse reaction.

Abstract:乌鸡白凤丸系明朝《寿世保元》方，由人参、黄芪、白芍、当归、熟地、川芎、乌鸡、鹿角胶、天门冬、生地黄、鳖甲、银柴胡、芡实、山药、丹参、香附、鹿角霜、桑螵蛸、煅牡蛎组成。其主要功用是益气养血，调经止带，主治气虚血亏引起的月经不调、崩漏带下、腰酸腿痛、身体消瘦、阴虚盗汗，尤以对阴虚血热、月经提前、气虚崩漏、脾虚白带过多，产后恶露不止等症疗效更为显著。笔者将其用于治疗遗尿、复发性口腔溃疡也取得较好疗效，此乃异病同治之理，现报道如下。[第一段]

Abstract:Objective：To investigate the resources of medicinal microorganisms of the East China Sea. Methods： The sea water and sediment samples were collected from the East China Sea. Different culture media were used to isolate the microorganism strains by infinite dilution and streak plate procedure. Then the isolated strains were screened for antimicrobial active strains using paper disc diffusion model and for antimitosis active strains using Pyricularia Oryzae model. Results： A total of 1 041 strains of marine microorganisms were isolated from marine water and sediment samples, including 506 actinomycetes, 114 fungi and 421 bacteria. It was found that 303（29.1 %） strains of marine microorganisms had antimicrobial activity and 219 （21.0%） strains had antimitotic activity. Conclusion.. The proportions of strains with antimicrobial activities and antimitotic activities are both above 20%, suggesting that the East China Sea has rich resources of medicinal microorganisms.

Abstract:Chronic insomnia is a severe disease that seriously influence the human health, and about 10%-15% of the adults suffer from it. No definite conclusion has been made about its etiology up to now, possibly associated with heredity, hormone secretion and living habits. Insomnia not only decreases the patients＇ quality of life,but also adds burden to society, families and individuals. The diagnosis of chronic insomnia should be based on the patients＇ sleep history, medication history, psychiatric history and necessary examinations. International diagnosis criteria should be combined if possible. Presently the treatments for chronic insomnia mainly include the OTC medicine, prescription drugs,self-medication with alcoholic beverage, cognitive behavior therapy, melatonin and some traditional herbal therapies. Limited information is available presently about insomnia and a large amount of laboratory and clinical research need to be done to further understand and solve this public problem

Abstract:Desmoid tumor （DT）, a rare benign tumor sometimes with local invasion, is more frequently seen in patients with familial adenomatous polyposis （FAP） than in the general public, which might be associated with surgical trauma, genetic predisposition and hormonal factors. Fifty percent of FAP-associated DTs are intra-abdominal, usually present as non-tender, slowly growing masses. The symptoms include abdominal pain, vomiting and diarrhea, etc. Mesenteric DT can cause obstruction and ischemia of intestine, hydronephrosis and fistula, Diagnosis can be made through biopsy or CT scanning. Surgery should be considered for the abdominal wall DTs and medicine is the first choice for mesenteric DTs, Surgery should be chosen with caution

Abstract:Objective：To study the factors influencing the amplification of sequence-related amplified polymorphism（SRAP） system in Carthamus tinctorius L. and to establish a stable SRAP reaction system, laying a foundation for molecular marker assistant breeding of Carthamus tinctorius L. Methods： Cetyltrimethylammonium bromide method was used to extract the genomic DNA of Carthamus tinctorius L.. Twenty-seven tests with 3 factors at 3 levels were designed, the conditions including Taq polymerase concentrations （0.02,0.04,0.06 U/μl）, dNTP concentrations （ 0. 15,0. 25,0.30 retool/L） and Primer concentrations （0.15,0.30,0.45 μmol/L） ;another 8 tests were designed based on the single factor of Mg^2＋ concentrations（0.5,1.0,1.5,2.0, 2.5,3.0,3.5 and 4.0 mmol/L）. Twenty ng DNA template was added into 25 μl SRAP reaction system; the system was optimized and agarose electrophoresis was used for determination. Results： A SRAP reaction system for Carthamus tinctorius L. was established. In a 25 μl reaction system, Taq polymerase was 0.02 U/μl, dNTP was 0.25 mmol/L,Primer was 0.30 μmol/ L, and Mg^2＋ was 3.0 mmol/L. Target bands increased after optimization, with good reproducibility, and the amplification results were satisfactory. Conclusion： The SRAP reaction system in this experiment is suitable for analysis of Carthamus tinctorius L.

Abstract:1临床资料 患者女性，33岁，因全身反复出现红斑、脓疱伴发热11年，加重半个月入院。1994年6月妊娠5个月时，无明显诱因颈部皮肤突然出现大片不规则红斑，红斑表面很快出现米粒至绿豆大小密集的小脓疱，轻度瘙痒，皮疹迅速扩及躯干、四肢，成批出现，此起彼伏，伴高热、头晕头痛、全身不适等症状，体温最高40．5℃，在外院经皮肤组织病理学检查确诊为“疱疹样脓疱病”，给予地塞米松等治疗（具体剂量不详）5个月，症状缓解。此后类似皮损反复出现，多由内服或外用药物引起，自服中药后皮损可消退，但不能控制复发。2005年5月底，因皮肤擦伤外用“皮康”治疗，全身皮肤再次出现红斑、脓疱，高热，在当地医院静滴地塞米松10mg／d，左氧氟沙星0．2／d，治疗2d，病情无明显好转，遂来我院就诊，门诊以“疱疹样脓疱病”收入院。此次发病患者精神差，睡眠饮食欠佳，大小便正常。体格检查：体温38．8℃，脉搏96次／min，急性面容，精神差，系统检查未见异常。皮肤科检查：颈部、躯干、四肢弥漫性红斑，其上可见密集排列的米粒至绿豆大小脓疱，尤以颈部、乳房下、腹部、四肢屈侧皮肤为重，部分脓疱融合成脓湖，脓疱周围有结痂、脱屑及色素沉着，双下肢高度凹陷性水肿，自觉皮肤灼痛。全身黏膜未见损害。双手足指趾甲未见异常。毛发无脱落。[第一段]

Abstract:Objective：To establish an Ehrlich reaction-colorimetry method for determining furostanol saponins in saponins extract from Radix Asparagi. Methods： Based on the coloration reaction between furostanol saponins and p-dimethylaminobenzaldehyde, a colorimetry method was established to determine the content of furostanol saponins in saponins extract from Radix Asparagi at 510 nm. Saponins AC- 9 was measured as reference in the determination. Results： The standard curve of saponins AC-9 showed good linearity in the range of 0.08-0.40 mg and the average recovery was 99.79% （RSD= 1, 05% ）. Conclusion.. This method is rapid, accurate and easy to be carried out, and it can be used to control the quality of Radix Asparagi

Abstract:白念珠菌是能感染人体的重要微生物之一.由于白念珠菌的遗传学操作较困难,因此其基因功能的研究主要依赖于酿酒酵母作为模型或替代性表达宿主.近年来,以白念珠菌自身作为宿主的基因中断技术逐渐成为研究其基因功能的主要手段,本文对这一领域的研究进展作一综述.

Abstract:目的:建立全颈椎(C1～T1)三维有限元模型.方法:根据一健康成年男性志愿者的颈椎CT与CT重建片,采用断层CT扫描序列图像的自动重建方法,建立全颈椎(C1～T1)三维有限元模型.结果:本研究成功的建立了全颈椎三维有限元模型,它包括C1～T1共8个椎体,本模型高度模拟颈椎结构与材料特性,结构完整,空间结构的测量准确度高,单元划分精细,共有节点数166 979,单元数12 177.结论:所建立的全颈椎有限元模型可以用来进行颈椎生物力学实验.

Abstract:目的:探讨扩张型心肌病患者纤溶功能的变化以及伊贝沙坦和依那普利对其的影响.方法:扩张型心肌病患者54例,按随机单盲平行的原则分为3组:20例服用伊贝沙坦50～75 mg qd,20例服用依那普利5～10 mg qd,14例服用安慰剂,疗程4周.治疗前后检测纤溶酶原激活物抑制剂1(PAI-1)和组织型纤溶酶原激活物(t-PA)活性,并予超声心动图测定左室收缩末期内径、左室舒张末期内径、射血分数和左室短轴缩短率的指标变化.健康体检者30例作为对照.结果: 扩张型心肌病组PAI-1活性明显高于正常对照组[(1.12±0.14) vs (0.56±0.16),P<0.05],t-PA活性明显低于正常对照组[(0.22±0.09) vs (0.57±0.11),P<0.05];伊贝沙坦和依那普利治疗后,PAI-1降低,t-PA水平增高 (P<0.05),并且左室收缩末期内径、左室舒张末期内径和射血分数较治疗前有改善(P<0.05).结论:扩张型心肌病患者存在血纤溶功能异常,伊贝沙坦和依那普利可改善扩张型心肌病患者纤溶功能失调,并且改善心功能.

Abstract:目的:调查分析内科住院患者中各类心脏瓣膜病的构成及分布.方法:2002年8月至2004年7月经彩色超声多普勒检查的3 560例内科住院患者中共检出1 250例心脏瓣膜病作为研究对象,按年龄分组,据超声结果及临床资料分为风湿性、钙化性、缺血性、钙化性合并缺血性、先天性及其他等6种类型,统计各类瓣膜疾病例数、构成比及在各年龄组的分布.结果:1 250例心脏瓣膜病例,男性667例,女性583例.年龄22～95岁,平均(72.3±14.3)岁.钙化性瓣膜病923例(73.8％),其中发生在60岁以上组895例;其他类139例(11.1％);缺血性病变83例(6.7％),其中发生在60岁以上组70例;钙化性合并缺血性75例(6.0％);风湿性瓣膜病21例(1.7％);先天性9例(0.7％).结论:钙化性和缺血性瓣膜病已替代风湿性瓣膜病成为临床心脏瓣膜病的主要类型,两者均与增龄有关.

Abstract:目的:探讨影响原发性甲状腺恶性淋巴瘤预后的主要因素.方法:回顾性分析22例甲状腺恶性淋巴瘤患者的临床特点、组织病理学分型、治疗方法,并就其与预后的关系进行统计学分析.结果:22例患者3年生存率75％,5年生存率64.5％.低度恶性组9例,包括 (MZBL)8例和Ⅰ级滤泡性淋巴瘤 (FCL)1例,无1例死于该疾病;高度恶性组13例,均为弥漫性大B细胞淋巴瘤(DLBCL)患者,5例死于该疾病,5年生存率45.5％;ⅠE期9例,无死于该疾病患者,ⅡE期13例,5例死于该疾病, 5年生存率45.5％,两者均显示显著性差异(P<0.05).结论:组织病理学类型和临床分期可能与预后相关,手术结合术后放疗和化疗是治疗甲状腺恶性淋巴瘤的合理方案.

Abstract:目的:阐明两相关诊断试验的评价及比较的方法,为新的诊断试验的临床应用提供科学依据.方法:通过ROC曲线下面积来对两相关诊断试验进行评价和比较,用拟合双正态模型的参数法来估计ROC曲线下面积,并通过对前列腺癌的两种诊断试剂的分析来说明方法的应用.结果:采用ROC曲线的方法,得到的对照试剂两指标Free-PSA、Total-PSA ROC曲线下面积分别为0.92、0.90,检测试剂相应指标的ROC曲线下面积分别为0.91、0.89,2个试剂相应指标ROC曲线下面积的差异无统计学意义,经等效性检验Z值分别为2.73 、2.78, 相应的P值为0.006 4、0.005 4,有统计学意义.结论:2个诊断试剂的分析表明均具有较好的诊断性能,其诊断准确度相同,同时也表明采用ROC曲线下面积可方便地对两诊断试验进行评价和比较.

Abstract:目的:探讨茶苯海明速崩片的制备工艺.方法:茶苯海明口崩片的制备、含量测定、采用正交设计法以崩解时限、体外溶出度为指标筛选优化实验、含量均匀度、片重差异、硬度检查、稳定性考察.结果:用最小二乘法对光密度D与浓度c作线性拟合得标准曲线方程:D=0.022c-0.005,r=0.999.平均回收率为:(100.19±0.96)％ (n=3) 日内RSD<1％,日间RSD<2％.根据K值大小,找出各因素的最佳水平:A1B1C1,即交联聚乙烯吡咯烷酮(A)5％、甘露醇(B)10 ％、MCC (C)10％由R值大小可见各因素对溶出度的影响大小依此为:C > A > B,以此处方作为制备DMH口崩片的基本实验处方;随MCC的用量的增加而主药的溶出速度减小,特别是T50有较大的差别,故作为黏合剂和助流剂的MCC,本处方选用10％;随交联聚乙烯吡咯烷酮的用量的增加而主药的溶出速度减小,特别是T50有较大的差别,故作为崩解剂的交联聚乙烯吡咯烷酮,本处方选用5％.在酸性条件下,即pH值在1.2～6.8的范围内,药物溶出的T50基本相同.随着溶出介质的温度增大,溶出速率略有增加.结论:制备的茶苯海明口崩片工艺成熟、质量稳定.

Abstract:潜水医学，发生在中枢神经系统的减压病以脊髓减压病较为多见，在失事潜艇的艇员脱险时，往往由于没有立即救治的条件，使脊髓减压病成为减员的重要原因之一。本实验通过对脊髓减压病大鼠NGF，TrkA和TNF-α蛋白表达的观察，分析减压病致脊髓损伤的自身保护因素与加重损伤的因素，及其时间分布的特点，认识脊髓减压病的发病规律，为治疗脊髓减压病提供理论依据。[第一段]

Abstract:脊柱肿瘤手术是一类操作难度和风险都很大的手术，对麻醉也有很高的要求。我院自2000年以来，共完成脊柱肿瘤手术546例，在该类型手术的麻醉处理上积累了较为丰富的经验，现总结如下。[第一段]

Abstract:急性肾功能衰竭（acute renal failure，ARF）是心脏术后常见的严重并发症，ARF不仅使心脏手术患者住院日期延长，治疗费用增加，而且是术后死亡的独立危险因素。近年来持续肾脏替代疗法（CRRT）已经成为救治此类患者的有效手段。2000年7月至2005年12月我们应用CRRT治疗心脏术后严重ARF患者20例，现总结如下。[第一段]

Abstract:原发性甲状旁腺功能亢进症是一种内分泌功能异常疾病，容易被误诊或漏诊。本研究结合我院1989～2004年收治的9例甲状旁腺功能亢进症患者的临床资料，就其外科诊疗问题进行讨论。[第一段]

Abstract:急性肠梗阻并发粪性腹膜炎临床少见，但一旦发生，死亡率极高。我科自2000年1月至2005年10月收治老年急性肠梗阻并发粪性腹膜炎5例，现报告如下。[第一段]

Abstract:微孔多聚糖止血球（microporous polysaccharide hemospheres，MPH）是从植物淀粉中提取的一种微孔多聚糖，应用于活动性出血部位时，发挥分子筛作用，产生“瞬时凝胶（instant gelling）”，达到即刻止血的功效。国内外报道动物实验和临床使用止血效果均佳，对机体无不良反应，无迟发性出血发生。目前临床上广泛用于外科各种手术创面止血，取得较理想的效果。2005年1～7月在我院行重肝肝移植手术的患者术中采用MPH作为辅助止血治疗手段，取得了较为满意的效果，现报告如下。[第一段]

Abstract:泪小点开口狭窄是引起溢泪症状的一个主要原因。目前对其治疗有3种方法：一种为单纯泪小点扩张，但多因扩张后泪小点开口再度狭窄而失败；另一种方法为硅胶管植入法，但硅胶管保留时间长，给患者造成一定的痛苦；最近泪道激光成为治疗泪道阻塞疾病的一种新方法，但因其价格昂贵不能够普及。我科在2002年5月至2004年12月采用“三剪法”联合泪小点扩张治疗伴有溢泪症状的泪小点开口狭窄患者，取得一定疗效，现报告如下。[第一段]

Abstract:通心络胶囊是运用中医络病学说，在益气活血化瘀、搜风解痉通络的治法指导下，研制而成的中药复方胶囊，其主要成分为人参、水蛭、全蝎、蜈蚣、蝉蜕、土鳖虫、赤芍、冰片等，具有益气活血、通络止痛之功效。动物实验和临床试验均表明通心络能对急性心肌缺血产生保护作用。本研究以冠心病合并左心功能不全的患者为对象，观察在常规治疗的基础上，加用通心络治疗6个月后对患者左心功能的影响。[第一段]

Abstract:1临床资料患者，男性，32岁，因“车祸导致严重多发伤”急诊在全麻下行“双侧髂内动脉结扎、右股静脉破裂修补、右骶髂关节脱位复位内固定、左右耻骨骨折切开复位内固定、剖腹探查、直肠造瘘、膀胱造瘘、会阴裂伤修补、双臀撕脱组织清创引流、异体皮覆盖术”，术前曾有心搏骤停史，CPR成功后转入手术室抢救。入室后开放外周静脉，行右颈内静脉、桡动脉穿刺置管，此时BP45／20mmHg（1mmHg=0．133kPa），HR140次／min，SpO2测不出。罗库溴铵50mg、去甲肾上腺素50μg静推后行气管插管，接麻醉机行控制呼吸。术中连续监测有创动脉压、HR、ECG、SpO2、PetCO2、CVP、尿量和鼻咽温，定期复查动脉血气，采用输血加温和加压装置。1：30手术开始，11：30手术结束，液体总入量33350ml（其中红细胞悬液12400ml、血浆8000ml、晶体10000ml、胶体2500ml、血小板19单位、冷沉淀16单位、5％碳酸氢钠350m1），总出量24000ml（其中出血18000ml，尿量6000m1）。[第一段]

Abstract:1临床资料 患者，女性，26岁，因“确诊家族性腺瘤性息肉病19年”入院，病程中无明显心慌、饮食、睡眠、性格及体质量改变。体格检查发现甲状腺Ⅱ度肿大，左侧甲状腺可触及约2．0cm×1．5cm质中结节，无明显压痛性多发性结节，随吞咽上下活动，右侧甲状腺未及明显结节。B超见左侧甲状腺中部见-2．3cm×1．7cm圆形结节，边界清，伴囊性变，右侧甲状腺近峡部见0．7cm×0．4cm圆形结节，边界清，内部回声均匀，提示甲状腺多发结节伴部分囊性变。甲状腺ECT提示两叶增大较明显，左叶中部有一较明显的放射性减低区（凉结节），右叶放射性分布尚可。头颅CT未见明显异常，眼底检查未见先天性视网膜色素上皮肥厚，胃十二指肠未见息肉。[第一段]

Abstract:1临床资料 患者，女，58岁，因左眼视物不见44年以左眼巩膜葡萄肿入院。眼部检查发现左眼无光感。于2005年7月14日行左眼球摘除术，羟基磷灰石义眼座（美国奥尔公司生产进口珊瑚材料，孔径500μm）经自体巩膜壳包裹后被植入肌锥腔内，手术顺利。术后2周拆除结膜缝线。拆线后2d患者突然出现左眼部疼痛，眶周软组织及眼睑肿胀，下睑外翻，大量异常分泌物，呈淡黄色，黏稠，味臭，血白分升高。结膜囊细菌培养：表皮葡萄球菌阳性。给予头孢曲松钠2g，2次／d，治疗5d症状好转。但红肿症状仍明显，故收治入院。入院后给予局部托百士眼液、迪可罗眼膏，结膜囊冲洗，根据药敏结果予左旋氧氟沙星（来立信）0．2g，静滴，2次／d，症状好转不明显，并出现结膜伤口裂开，乳白色筋膜组织外露。3d后行羟基磷灰石义眼座取出，术中义眼座呈黄色，未血管化，去除巩膜壳，采用高压蒸气灭菌法灭菌30min，并将直径22mm义眼座切削至18mm，肌锥腔内用双氧水、庆大霉素溶液、生理盐水反复冲洗后将无巩膜壳包裹眼座重新置入眼内，缝合筋膜，结膜。结膜囊内油纱条充填。[第一段]