Abstract:Objective： To study the influence of glycogen synthase kinase 3β（GSK3β） overexpression on the function of murine macrophage RAW264.7. Methods： Eucaryotic vector pcDNA3. 1-GSK3β was constructed and transfected into RAW264.7 cells via lipofectamine 2000. RAW264.7 cells stably expressing GSK3β were screened by G418 selection. Phagocytosis ability of RAW264.7 cells was assessed by neutral red analysis; the content of nitric oxide （NO） and the activity of tumor necrosis factor （TNF） in the supernatant of RAW264. 7 cells stably expressing GSK3β were determined by Griess method and crystal violet staining assay, respectively. Results： RAW264.7 cells stably expressing GSK3β showed an inhibited phagocytosis ability,with the inhibitory rate being 20.4% （P〈0. 05）, and an inhibited production of NO by RAW264.7 cells stably expressing GSK3β in presence of 1 μg/ml LPS, with the inhibitory rate being 25. 6%（P〈0. 01）. The transfected RAW264.7 cells had an increased production of TNF by 28.8% （P〈0. 01）. Conclusion： GSK3β can regulate macrophages in inflammatory and immune responses.

Abstract:Objective： To study the expression of chemokine receptor CCR5 in follicular thyroid carcinoma and the serum level of CCR5 ligand, so as to assess the role of CCR.5 in progression and metastasis of follicular thyroid carcinoma. Methods： Fifteen samples of follicular thyroid carcinoma, 17 samples of follicular thyroid adenoma and 12 adjacent normal samples were analyzed immunohistochemically for CCR5 expression. The sera concentrations of CCL3, CCL4 and CCL5 were measured by ELISA in all patients. Results： CCR5 was positive in follicular thyroid carcinoma samples, with the positive rate being 73. 33%, and was not detected in the follicular thyroid adenoma and the normal samples（P〈0.01）. The concentrations of CCL3 and CCL5 in the sera of follicular thyroid carcinoma patients were significantly higher than those of the other 2 groups （P〈0.05）. Conclusion： CCR5 is highly expressed in follicular thyroid carcinoma tissues and the concentrations of CCL3 and CCL5 are obviously increased in the sera of patients, indicating that CCR5 may play an important role in the pathogenesis of follicular thyroid carcinoma

Abstract:1 临床资料 例1，患者男性，75岁，因“反复皮下、口腔出血半年余，肉眼血尿1个月”于2006年12月11日入住我院。患者1年前有全身皮肤瘙痒，口服阿司咪唑后好转，但仍反复发作；6个月前反复出现全身多处瘀斑；40d前咽后壁出现大量串珠状小血疱；30d前出现肉眼血尿。入院查体；贫血貌，全身皮肤散在大片瘀斑，口腔黏膜可见血疱，球结膜出血。

Abstract:Objective：To investigate the effect of arterial baroreflex （ABR） on survival rate of rats with cecal ligation and puncture （CLP）-induced sepsis. Methods： Male Sprague -Dawley rats were divided into 2 groups., sham-operated rats （n=22） and sinoaortic denervated （SAD） rats （n= 22）. Four weeks after SAD rats were subjected to CLP-induced sepsis, the blood pressure and heart period （HP） were monitored for 12 hours in conscious state and the survival of rats was observed. Results.. Both the diastolic and systolic blood pressue gradually decreased after CLP. the HP shortened first and then drastically prolonged until the death of rats. At 12 h after CLP the survival rate of SAD rats was lower than that of the sham operated rats （59 % vs 86%）. Significant differences were found between the Kaplan-Meier survival curves of the rats in 2 groups（P〈0.05）. Conclusion： ABR plays an important role in CLP-induced lethal sepsis model

Abstract:1 临床资料 患者男性，73岁，因慢性肾功能衰竭（尿毒症期）于2006年1月10日在我院行同种异体肾移植术，既往无外伤及肿瘤病史。术后采用环孢素A＋霉酚酸酯＋泼尼松三联免疫抑制方案，术后恢复良好，治愈出院。术后3个月，患者左前臂出现红色条状红斑，无痛、痒等不适，后自觉红斑逐渐增大，并高出皮肤，转为紫红色，同时颈部也出现类似红斑两处。术后4个月时患者发现红斑周围出现皮肤破损，[第一段]

Abstract:Objective： To compare the effects of electret and chemical enhancers in enhancing the transdermal delivery of meloxicam. Methods： The study was divided into 4 groups, including meloxicam patch group （control）, chemical enhancer ＋ meloxicam patch group,electret meloxicam patch,and electret ＋ chemical enhancer ＋ meloxicam patch group. The in vitro skin permeation of meloxicam from patches was examined by using a modified Franz diffusion cell. Ultraviolet spectrophotometry （362 nm） was used to analyze the drug concentration in the receptor. The 10 h-cumulated permeation amount of meloxicam from patchs was calculated in 3 experimental groups and were compared with that the control group. The enhancing abilities of electret and chemical enhancers were compared and the synergistic effect of them was assessed. Results： （1） 1 %. 3 % and 5 azones showed 1.20,1.33 ,and 1.26 fold-increases in the 10 h cumulated permeation of meloxiam respectively as compared with control group（P〈0.05）. （2） Most of the chemical enhancers used in thisstudy（1%,3% and 5%azones,10% ethyloleate,1% menthnol,and 300% sulphoxiade） ,except for 20 % propylene glycol,had enhancing effect on transdermal delivery of melocicam. Ethyl oleate（10%） was proven to be the most potent enhancer, showing a 1.86 fold cumulated permeation that of the control group（P〈0. 05） ; （3） Electret was more potent than the chemical enhanrers in promoting meloxieam transdermal delivery, showing a 2. 16-fold cumulated permeation that of control group（P〈0. 05）;（4） Electret improved the enhancing effect of chemical enhancers in this study. The cumulated permeation of meloxicam in electret ＋ chemical enhancer ＋ meloxicam patch group was 1.14-2.82 folds that of the chemical enhancer ＋ meloxicam patch group（P〈0. 05）. Conclusion： Electret has a good promoting effect for transdermal drug delivery and can be used as a novel enhancer in transdermal delivery of drugs

Abstract:Objective： To investigate the variation of visual acuity （VA） at different contrast levels under bright and dark backgrounds in healthy adults. Methods： Fifty-four men （ 108 eyes） with normal VA were enrolled in this study. LogMAR VA was measured at 4 contrast levels （100 %, 25 %, 10%, and 5 % ） under bright and dark backgrounds with multi-functional visual acuity tester （MFVA-100）. The relationship of VA between bright and dark backgrounds was analyzed. Results： （1） Subjects with lower contrast level had significantly worse VA than those with higher contrast level when the background was identical （P〈0. 001）; VA decreased gradually with the decrease of contrast level. At the same contrast level, the VA under dark background group was significantly worse than that under bright background group （P〈0. 001）. （2） The VA at contrast level 100% under bright background was not linearly related to those under dark background （P〉0.05）, but was linearly related to those at other contrast levels under bright background （P〈0. 001）. Under dark background, the VA at contrast level 100% was linearly related to those at other contrast levels（P〈0. 001）. （3） The fluctuating differences （log unit） of VA increased with the decrease of contrast level, with the maximal difference found at contrast level 5 ; under dark background. Conclusion： The VA of healthy men under bright background is better than that under dark background. The VA decreases and individual variation increases gradually with the decrease of contrast level. There is no linear correlation of VA between the bright and dark backgrounds.

Abstract:Objective： To screen,identify and classify an antimitosis marine bacterium strain 32-11-2-2 of Eastern China Sea. Methods： The marine bacteria were isolated with the infinite dilution and plate streak technique and screened for antimitotic active strains by Pyricularia Oryzae model. The active strain 32-11-2-2 was subjected to morphological, physiological and biochemical study, and was classified by the analysis of the 16S rDNA sequence. Results： A total of 421 strains of marine bacteria were isolated from marine water and sediment samples; 54 of them had antimitotic activity. The study of morphological, physiological and biochemical characteristics showed that the strain 32-11-2-2 was a slight halophilic strain; it had the morphological characteristics of a continent Bacillus and could produce amylase, fluidify glutin; and it also showed a positive reaction in the litmus milk test. The 16S rDNA analysis showed the strain 32-11-2-2 had a 98% homology with Bacillus subtilis. Conclusion： The strain 32-11-2-2 has been identified to be a Bacillus subtilis adapted to the oceanic environment.

Abstract:Adenovirus as gene transfer vector is characterized by high transfection efficiency, high titer,good stability in target cells and low pathogenicity, and has been widely used in gene therapy. Compared to first- and second-generation adenovirus vectors, the third-generation adenovirus vectors have unique advantages for gene therapy because of their low immunogenicity and high capacity. However, the clinical application of the third-generation adenovirus vectors is hampered by the existance of helper contamination and difficulties in their large-scale production

Abstract:Cryptococcus neoforrnans is an important conditional pathogenic fungus; the capsular polysaccharide surrounding its outer wall is the first identified major toxic factor of Cryptococcus neoforrnans. This review summarizes the progress in the research of capsule characteristics and dynamics of Cryptococcus neoforrnans, including the structural changes, tissue constitution, development and growth,etc,hoping to provide a basis and new ideas for studying Cryptococcus neoforrnans related diseases

Abstract:目的：寻找高效抗真菌化合物，探讨三唑醇类化合物的构效关系。方法：根据三氮唑类抗真菌药物的构效关系和作用机制，设计合成了14个1-(1H-1,2,4-三唑-1-基)-2-(2,4-二氟苯基)-3-芳氧基-2-丙醇类化合物，用微量液基稀释法测定化合物对白念珠菌、新生隐球菌、近平滑念珠菌、热带念珠菌、红色毛癣菌、羊毛状小孢子菌、薰烟曲霉菌和紧密着色真菌8种临床常见致病真菌的体外抑菌活性(MIC80)。结果：以氧原子替代侧链中的硫原子后，侧链中含有邻位取代苯基的化合物的总体抗真菌活性不高，而侧链含有间位或对位苯基的化合物表现了很好的体外抗真菌活性。结论：间位或对位取代苯基的引入有利于此类化合物的抗真菌活性。

Abstract:目的：研究VEGFR2抑制剂与靶酶的作用机制,为设计新型抑制剂建立理论模型.方法：应用AutoDock3.05对接2-吲哚酮类抑制剂到靶酶的活性腔,然后以抑制剂的对接构象为基础,在Sybyl6.9中建立抑制剂的比较分子力场分析法（comparative of molecular field analysis, CoMFA）和比较分子相似性指数分析法（comparative of molecular similarity index analysis, CoMSIA）三维定量构效关系模型.结果：对接构象与文献报道结果一致,三维定量构效关系统计指标Q2均大于0.5.结论：所得结果具有较好的可靠性,对于设计新型化合物具有指导意义.

Abstract:目的：考察活性氧对白念珠菌多药耐药基因CaMDR1的转录和耐药表型的影响,以及Cap1p 在此过程中的作用.方法：应用Northern印迹方法考察了H2O2对白念珠菌SC5314、Y0109的CaMDR1转录水平的影响;采用白念珠菌CAP1 基因正常表达菌CAI4、CAF2-1,及CAP1 基因缺失菌CJD21、XZY6,用Northern印迹方法考察Cap1p在H2O2激活CaMDR1转录过程中的作用;应用微量液基稀释法考察H2O2对白念珠菌耐药表型的影响.结果：H2O2能够激活白念珠菌SC5314、Y0109中CaMDR1的转录表达;H2O2刺激后,白念珠菌CAP1基因正常表达菌CAI4和CAF2-1中CaMDR1的转录水平都有明显升高,而CAP1基因缺失菌CJD21和XZY6中CaMDR1的转录水平未见升高;H2O2可导致白念珠菌SC5314对氟康唑耐药性的产生,而菌株CJD21、XZY6对氟康唑的耐药程度未见升高.结论：活性氧以Cap1p依赖的方式激活白念珠菌CaMDR1转录,并导致耐药表型的出现.

Abstract:目的：检测类风湿性关节炎（rheumatoid arthritis,RA）患者外周血T杀伤细胞（cytotoxic T lymphocyte, CTL）和自然杀伤细胞（NK）中颗粒酶素B和穿孔素的表达,探讨其可能的临床意义.方法：取25例健康者、43例RA患者（31例活动期、12例稳定期;初发16例、复发27例）外周血及其中15例患者（初发8例、复发7例）关节液标本.采用三色流式细胞术胞内检测外周血及关节液标本中CTL、NK细胞颗粒酶素B和穿孔素的表达.结果：RA患者关节滑膜液中CTL和NK细胞颗粒酶素B及穿孔素的表达显著高于RA患者健康者外周血（P＜0.01或P＜0.05）.活动期RA患者外周血CTL和NK细胞表达颗粒酶素B、穿孔素明显高于稳定期RA患者（P＜0.01或P＜0.05）.初发和复发患者关节液及外周血中二者表达无显著差异.结论：RA患者外周血及关节液中CTL和NK细胞颗粒酶素B及穿孔素的表达显著高于正常人外周血,对其水平的检测利于判断疾病活动程度,但对判断疾病初发、复发的意义不大

Abstract:B型利尿钠肽（B-type natriuretic peptide，BNP）是一种由32个氨基酸构成的多肽类神经激素，主要来自于心室，作为一种心衰标志物在临床逐渐被接受。本研究拟以先前已制备的TRX—BNP融合蛋白作抗原，制备BNP单克隆抗体，为研制BNP单抗试剂盒作准备。

Abstract:额骨位于头颅前端，位置表浅．易受外伤而骨折甚至导致额窦破裂，且常合并硬脑膜及脑组织的损伤．出现脑脊液鼻漏甚至颅内感染等严重并发症．通常还伴随威胁生命的多系统损伤。因此，外伤引起额骨骨折和额窦破裂后及时合理的处理就显得非常重要。外伤引起的额骨骨折和额窦破裂治疗重点是破窦处理，但是额窦壁骨折引起寞破裂的治疗至今仍是一个有争议的课题

Abstract:过去100年，内分泌系统的研究主要集中在腺体结构及功能、激素生化结构及生理功能、内分泌激素的调节等。后来人们发现，其实激素的水平与其生理功能状态并不完全相同，激素的生物效应及功能不仅与激素水平有关，与受体及信号转导系统关系更密切，于是该领域逐渐转向研究激素的反应性，即激素受体及信号转导系统。这是一个机遇，也是一个挑战，我们应抓住机遇率先开展受体及信号转导系统的科研工作，[第一段]

Abstract:长期以来，癌症是世界上最重要的致死因素之一。流行病学数据表明，在过去的数十年中，每年死于癌症的人口达到将近7百万。据估计，到2020年，每年的新发癌症病例与因肿瘤致死人口将分别达到1600万与1千万。[第一段]

Abstract:心脑血管病、糖尿病血管并发症等已成为严重危害人类健康的重大疾病，加强其防治研究已成为我国重大疾病防治的主要任务。络病理论是中医理论体系的独特组成部分，络脉是从经脉支横别出，逐层细分，纵横交错，遍布全身，广泛分布于脏腑组织间的网络系统，把经脉运行的气血津液输布、弥散、渗灌到脏腑周身，是维持生命活动和保持人体内环境稳定的网络结构。络病是广泛存在于多种难治性疾病包括血管病变中的病理状态，[第一段]

Abstract:多年来，肥胖症的分子发病机制一直不清楚，直到1994年瘦素（leptin）ob基因和1995年瘦素受体（OB-R）基因的成功克隆，才使肥胖症分子发病机制的研究进入了新的时代。现介绍ob基因、瘦素受体及其信号转导的研究情况。[第一段]

Abstract:褪黑素（melatonin，Mel）是松果腺分泌的神经内分泌激素，其分泌呈昼夜节律，而松果腺是生物钟的重要组成部分。近年发现，Mel生物学作用十分广泛，有镇静、催眠、抗衰老、免疫兴奋、调节内分泌功能等。研究显示，这些作用可能是通过抗氧化来实现的。我们实验室验证人神经组织及脊髓、外周器官存在褪黑素受体（melatonin receptor，MR），为Mel广泛生物学作用机制的阐明奠定了坚实的理论基础。[第一段]

Abstract:尾加压素Ⅱ（urotensin Ⅱ，U Ⅱ）最早是自硬骨鱼的脊髓尾部下垂体中提取的一种生长抑素样环肽，它属于神经肽范围。后来证实其分布具有种属普遍性，从软体动物到哺乳动物的神经系统中均有存在。1996年，Coulouarn等从人体中克隆出UⅡ。随后，Ames等首次报道心血管组织中富含UⅡ，并且人体中有UⅡ的特异性受体，即一种孤立的G蛋白偶联受体（GPR14），[第一段]

Abstract:Objective：To investigate the influence of C-type natriuretic peptide（CNP） on the expression of natriuretic peptide B receptor（NPR B） and its cGMP signal induced by advanced glycation end products（AGEs） in human mesangial cells（HMC）. Methods： HMCs were cultured in vitro. The expression of NPRB, intracellular concentration of cGMP and level of NF-κB p65 in HMC were measured by immunofluorescent staining, enzyme linked immunosorbem assay（ELISA） and flow cytometry, respectively. Results： CNP significantly upregulated the immunofluorescent intensity of NPR-B and the concentration of cGMP inhibited by AGEs（P〈0.001） ;CNP also inhibited the fluorescent intensity of NF-κB p65 stimulated by AGEs. Conclusion： CNP can regulate the expression of NPR-B/ cGMP and activity of NF-κB induced by AGEs through a NPR- B/cGMP dependent manner.[著者文摘]

Abstract:Objective： To observe effects of different sulfonylurea compounds on expression of sulfonylurea receptor 2 （SUR2）in myocardium of the Goto-Kakizaki（GK）rats. Methods： Spontaneous diabetic GK rat models were divided into 6 groups： the diabetes model group, the Glibenclamide group, the Glipizide group, the Gliclazide group, the Glimepiride group and the positive control group（treated with insulin）, with 12 rats in each group. A normal control group was also set up for comparison. The expression of SUR2 in myocardium of the GK rats was investigated by radioligand binding assay. SUR2 mRNA expression in the myocardial cells of rats was detected through reverse transcriptase-polymerase chain reaction （RT PCR）. Results： Twelve weeks later, no significant difference was found in the SUR2 receptor density（Bmax）and affinity（Kd） between the sulfonylurea treated groups and the other 3 groups （P〉0.05）. There was no significant difference in SUR2 mRNA expression between the diabetic, insulin-treated diabetic and control groups （P〉0.05）. Cardiac SUR2 mRNA levels were not significantly different between sulfonylureas treated diabetic and non-treated diabetic rats （P〉0.05）. Conclusion： The diabetes itself does not affect the sulfonylurea receptor（SUR2） expression in myocardial tissues. Sulfonylureas at treatment dosage have no effect on receptor expression of SUR2.[著者文摘]

Abstract:Objective： To study the common single nucleotide polymorphism （SNPs） of sulphonylurea receptor 1 （SUR1） gene （16-3c/t and S1369A） and its relationship with the glucose-reducing effect of gliclazide. Methods; A total of 115 patients with type 2 diabetes were enrolled in this 8-week, open-label, cohort study. All patients were required to take gliclazide for 8 weeks. FPG, HbAlc and insulin were assayed before and after therapy and HOMA-B and HOMA IR indices were calculated to assess the therapeutic effects of gliclazide. The gene polymorphism of SUR1 was analyzed by Taq-Man technology and the effects of gliclazide were compared between patients with different phenotypes. Restults： （1） The study was completed in 101 of the 115 patients and the frequencies of c and t alleles were 0.54 and 0.44, A and S were 0.58 and 0.42, respectively. （2） FPG, HbAlc and HOMA B indices were significantly improved after therapy in patients with all kinds of genotypes （P~0. 05）; HOMA-IR indices had no significant change after therapy（P〉0.05）. （3） The changes of HbAlc and HOMAB indices of t/t group were more obvious than those of c/c and c/t groups （P〈0.05）. S1369A polymorphism had no association with the effect of gliclazide（P〉0.05）. Conclusion： The polymorphism of SUR1 can influence the glucose-reducing effect of gliclazide and this influence might be associated with loci of SNPs.[著者文摘]

Abstract:Objective： To observe the serum testosterone （T） level and androgen receptor （AR） mRNA expression in peripheral leukocytes and thyroid tissues of patients with Graves disease, so as to investigate the relationship between the testosterone and androgen receptor with hyperthyroidism. Methods： Blood samples were collected from 8 female patients with Graves disease,8 female patients with thyroid adenoma and 8 healthy control subjects; the patients were paired in gender and age. The plasma concentrations of testosterone were determined by enzyme-linked immunosorbent assay （ELISA） ; the [^3 H]-T specific binding sites in the peripheral leukocytes were determined by radioligand binding assay; the expression of AR mRNA in peripheral leukocytes were determined by RT-PCR; and the level of AR in thyroid tissues was determined by immunohistochemistry method. Results： The plasma concentrations of T were （660±80） ng/L and（620± 100） ng/L in Graves disease group and control group, respectively （P〉 0. 05） ; the [^3 H]-T specific binding sites were （ 381 ± 105） sites/cell and （572 ± 141 ） sites/cell in peripheral leukocytes, respectively （ P〈 0.05 ）. The mRNA expression of AR in Graves disease group was significantly higher than that in the control group（P〈0.05）. The result of immunohistochemistry showed that the level of AR in thyroid tissues was higher in Graves group than that in the control group. Conclusion： The plasma level of T in patients with Graves disease is similar to that of normal controls, but the mRNA expression of AR in peripheral leukocytes and thyroid tissue is higher in Graves disease group than in the control group, which is supposed to be a protective reaction. Moreover, the binding capability of AR is lower in Graves disease group than that in the control group, which is probably caused by the accelerated decomposition of AR.[著者文摘]

Abstract:Objective： To observe the effects of different concentrations of glucose on the expression of tumor necrosis factor-related apoptosis-inducing ligand （TRAIL）, osteoprotegerin （OPG）, and the ligand of osteoprotegerin （OPGL） in osteosarcoma MG63 cells, so as to assess the role of glucose in pathogenesis of diabetic osteoporosis. Methods： MG63 cells were incubated with glucose at the concentrations of 5.5,16.7,33.3 mmol/L for 24 h. The expression of TRAIL, OPG and OPGL mRNA were examined by RT-PCR. Expression and distribution of TRAIL in MG63 cells were investigated by immunohistochemical method. Results： The mRNA expression of TRAIL and OPGL in MG63 cells increased in the order of control group, 5.5,16.7,33.3 mmol/L glucose groups（ P〈 0.05）, and the expression of OPG mRNA decreased in the same order（P〈0.05）. TRAIL mRNA level in 33. 3 mmol/L glucose group was significantly higher than that in the control group [（1. 004±0. 070） vs （0. 740±0. 023）,P〈0.05] and the expression of TRAIL was stronger than that in the control group. Conclusion： High concentration of glucose can lead to increased expression of TRAIL and OPGL in osteoblasts and decreased expression of OPG, which may be one of the key pathogenetic factors of diabetic osteoporosis.[著者文摘]

Abstract:Objective： To extract the syndrome elements of CHD angina pectoris with entropy-based complex system partition method and to analyze the distribution of the syndrome elements, in an effort to study the pathogenetic characteristics of CHD angina pectoris. Methods： The clinical data of 403 patients with CHD angina pectoris were collected by clinical epidemiological survey. Entropy-based Complex System Partition was used to extract the syndrome elements, contribution degree of symptoms to syndrome and diagnostic threshold. The combination rule of the syndrome elements was also analyzed. Results： CHD angina pectoris had the following basic syndromes： Qi Deficiency syndrome, Qi Stagnation syndrome, Blood Stasis syndrome, Phlegm Turbid syndrome, Phlegm-fire syndrome, Stagnation-fire syndrome, Yin Deficiency syndrome and Yang Deficiency syndrome. With the increase of syndrome elements, the frequencies of Qi Deficiency syndrome, Yin Deficiency syndrome, Qi Stagnation syndrome, Blood Stasis syndrome, and Phlegm turbid syndrome also increased. Qi Deficiency syndrome and Blood Stasis syndrome were the two syndrome elements seen most frequently. Conclusion： The syndrome elements extracted by entropy-based complex system partition method and their distribution rule can reflect the pathogenic characteristics of angina pectoris, which lays a base of quantify the diagnostic criteria of the angina pectoris syndrome.[著者文摘]

Abstract:Objective.. To investigate the application of entropy partition method （EPM） for studying the diagnostic criteria of Traditional Chinese Medicine （TCM） syndrome. Methods.. A clinical epidemiological survey was conducted in 400 patients with vascular endothelial dysfunction and the EPM was adopted to extract the TCM syndrome elements of ＂stagnancy of collateral-Qi in a deficiency condition＂, the correlation degree between each symptom of ＂stagnancy of collateral-Qi in a deficiency condition＂ was calculated and the diagnostic threshold of vascular endothelial dysfunction was established. The sensitivity, specificity and accuracy of the EPM-associated diagnostic criteria were assessed by comparing with those of clinical epidemiology. Results： The diagnostic criteria of ＂stagnancy of collateral-Qi in a deficiency condition＂ in vascular endothelial dysfunction established with EPM had a satisfactory diagnostic efficiency. Conclusion： EPM for complex system is suitable for studying the diagnostic criteria of TCM syndrome. EPM is a scientific and strict method and has great value in clinical practice.[著者文摘]

Abstract:Objective：To investigate the vascular protective mechanism of different levels of herbs through detecting activity of HO-1/CO system in arterial tissues of rats with stagnancy of collateral-Qi in deficiency condition. Methods： Healthy male Wistar rats of cleanness level were randomly divided into control group, stagnancy of collateral-Qi in a deficiency condition group （SCQDC group）, Renshen group, Shuangshen group, and Tongxinluo group. The ultrastructure of endothelium cells in the artery was observed. RT-PCR, Western blot, and immunohistochemical approaches were used to detect JNK, c-Jun mRNA and albumen expression in the arterial tissues. Meanwhile, the content of CO in the arterial tissue was also detected. Results： In SCQDC group, the counts of pinocytotic cells and microvili on the free surface of vascular endothelial cells were significantly decreased; most crests of mitochondria fused with membrane, some even disappeared; serious degranulation was observed in the rough surfaced endoplasmic reticulum. Immunohistochemistry showed that the HO-1 positive signals in the arterial histiocyte weakened significantly compared with the control group. The HO-1 expression was increased in the 3 treatment groups. Compared with control group, SCQDC group had decreased HO-1 mRNA and albumen expression（P〈0.01, P〈0.001 ）. The 3 different kinds of Herbs to dredge collaterals increased the HO-1 mRNA and albumen expression to different degrees （P〈0. 001）. The increase of albumen in the Tongxinluo group was more than those in the Shuangshen group and the Renshen group（P〈0. 001）. Tongxinluo also significantly increased the CO content. Conclusion： Herbs to dredge collaterals can induce CO production by ameliorating or recovering the HO System, which may be one of the mechanisms for protecting the vascular tissues.[著者文摘]

Abstract:目的：获得高比活度的核素标记药物。方法：试用无水碘标记法标记磺脲类药物格列本脲，并应用放射配体结合分析法测定大鼠心肌组织膜磺脲类药物受体的特性。结果：所获得的^125I-格列本脲放射比活度较高（111GBq／mmol），放化纯度为95．6％，^125I-格列本脲可和大鼠心肌组织膜上磺脲类药物受体结合。结论：无水碘标记法是非水溶性物质核素碘标记法的理想方法之一。[著者文摘]

Abstract:目的：探讨甲状腺功能亢进症（甲亢）患者性激素受体改变及意义.方法：应用放射配体结合法,以3H标记雌二醇（^3H-E2）和睾酮（^3H-T）为配体,测定甲亢患者白细胞E2受体（ER）和T受体的变化,同时采用酶联免疫分析法测定血浆E2和T浓度.结果：甲亢患者ER水平较正常对照组增高,甲亢治愈组ER均明显降低（P＜0.05）,无效者改变不大（P＜0.01）,复发者降低的ER有所恢复（P＜0.05）.结论：甲亢患者ER含量增高与Grave病（GD） 发病相关,雌激素可能为GD 的诱发因素之一;雌激素及其受体水平与甲亢疗效的关系尚待进一步深入研究.[著者文摘]

Abstract:目的：探讨女性冠心病患者性激素受体的改变及其意义.方法：将女性冠心病患者分为3组,分别为冠心病组、心肌梗死存活组、心肌梗死死亡组.应用放射配体结合法,以3H标记雌二醇（^3H-E2）和睾酮（^3H-T）为配体,测定各组患者白细胞雌二醇受体（ER）和睾酮受体（AR）的变化,同时采用放射免疫分析法测定血浆E2和T浓度.结果：女性冠心病患者E2受体较正常同年龄阶段女性对照组受体水平减低,而在三组冠心病患者中以心肌梗死死亡组E2受体明显降低（P＜0.01）,同时3组冠心病组ER/AR比值均明显降低（P＜0.01）.结论：提示女性冠心病患者E2浓度降低与其受体水平有关,研究女性冠心病患者性激素及其受体改变有助于采取综合措施来进一步预防冠心病的发生.[著者文摘]

Abstract:目的：探讨多发性肌炎/皮肌炎（PM/DM） 患者外周血白细胞糖皮质激素受体（GR）和血清微量元素的变化及其发病机制.方法：应用放射配体结合法,以^3H标记地塞米松（^3H-Dex）为配体,测定24例PM/DM患者外周血白细胞GR的变化,并采用放射免疫分析法测定血浆皮质醇浓度,同时采用原子吸收法检测血清微量元素（包括Zn、Cu、Fe、Ca和Mg）的水平.结果：PM/DM患者其外周血白细胞GR特异结合容量为（3 673±1 195） 位点/细胞,低于正常对照组的（4 966±1 210）位点/细胞,有显著性差异（P＜0.01）.PM与DM患者组间外周血白细胞GR特异结合容量无显著性差异（P＞0.05）.PM/DM患者其GR 水平与相对应的血浆皮质醇浓度之间无相关性（P＞0.05）.结论：PM/DM患者外周血白细胞GR水平的变化以及血清锌元素含量的降低与PM/DM的发病机制、应用肾上腺糖皮质激素治疗的疗效判定、预测预后等可能有密切关系,但尚需进一步深入研究.[著者文摘]

Abstract:目的：探讨鼠溃疡性结肠炎时糖皮质激素（GC）和糖皮质激素受体（GR）的相应改变。方法：Wistar大鼠和C57／L6小鼠共30只，随机分成3组（n=8）进行灌注实验。超速离心鼠肝、胃、结肠组织，取上清液，以^3H地塞米松（Dex）为配体，测定动物肝、胃、结肠组织血浆皮质醇及肝、胃、结肠组织GR结合量改变。结果：动物实验组血浆皮质醇明显升高（P〈0．05，P〈0．01），氢化可的松治疗组血浆皮质醇未继续升高；实验组肝、胃、结肠。H-Dex特异结合位点测定明显降低，治疗组则有所回升（P〈0．05，P〈0．01）。结论：溃疡结肠炎鼠血浆皮质醇明显升高，提示溃疡结肠炎属应激状态。肝、胃、结肠组织^3H-Dex结合量降低，经氢化可的松治疗后肝、胃、结肠组织^3H-Dex结合量明显回升，提示血浆皮质醇对GR之作用是正相关作用，为临床合理应用糖皮质激素治疗提供依据。[著者文摘]

Abstract:目的：观察不同葡萄糖浓度对人成骨肉瘤MG63细胞株护骨素（OPG）、护骨素配体（OPGL）及肿瘤坏死因子相关凋亡诱导配体（TRAIL）、巨噬细胞集落刺激因子（M-CSF）、转化生长因子-β（TGF-β）表达的影响。方法：MG63细胞经不同浓度（5．5、16．7、33．3mmol／L）葡萄糖处理后，用RT-PCR法检测上述基因表达情况。结果：高糖能下调MG63细胞中OPG及TGF—β的表达（P〈0．05），上调OPGL、M—CSF和TRAIL的表达（P〈0．05）。结论：高糖环境可能导致成骨细胞中OPG及TGF-β的表达减少，OPGL、M—CSF和TRAIL等细胞因子的表达增多，使破骨细胞的数目和活性增加，骨吸收增强和骨量丢失，这可能是糖尿病骨质疏松症的一个重要的发病机制。[著者文摘]

Abstract:目的：证实兔及大鼠不同脑区存在^125I褪黑素结合位点。方法：采用Scachard分析方法检测兔及大鼠大脑及脊髓^125I褪黑素结合最大结合容量（Bmax）及平衡解离常数（K4），动力学分析研究^125I褪黑素解离过程，以及药物对^125I褪黑素特异结合的影响。结果：兔及大鼠视网膜特异结合的Bmax最高，视交叉及嗅球次之；下丘脑、海马及脑干也较多；动力学分析表明褪黑素与其受体为可逆性结合；特异性结合分析提示对褪黑素呈高度特异性。结论：兔及大鼠脑和脊髓组织存在^125I褪黑素结合位点。[著者文摘]