Abstract:Objective To investigate the effect of visfatin on the NG2 cell number in the brain of normal rats and rats with cerebral ischemia/reperfusion (I/R) injury. Methods Transient focal cerebral I/R model was established by middle cerebral artery occlusion (MCAO) and a monofilament suture via intraluminal approach in rats. Thirty adult male Wistar-Kyoto rats were randomly divided into four groups, namely, a sham operation group (Sham), a cerebral I/R model group(MCAO), Sham+FK866(a specific inhibitor of visfatin) group, and cerebral I/R+FK866 group. Animals in the latter two groups were intragastrically given FK866 (1 mg/[kg·d]) for a consecutive of 14 days; Sham operation or MCAO was performed at the 7^th day of FK866 treatment. The NG2 cells were observed by immunofluorescence staining in animal brains at the 7^th day after surgery. Results There was no significant difference in NG2 cell numbers between the Sham group and Sham + FK866 group. Cerebral I/R model group had significantly less NG2 cells in the core region of infarction(P<0.01) and significantly more in the penumbra area (P<0.01) compared with the Sham group, with no significant change in the contralateral side. The NG2 cell numbers were not significantly different between the cerebral I/R model group and cerebral I/R + FK866 group in the above mentioned areas. Conclusion Inhibition of visfatin shows no noticeable effect on NG2 cell number in the brains of normal and cerebral I/R rats, indicating that the neuroprotective effect of visfatin is not associated with NG2 cells.

Abstract:Objective To analyze whether human-derived ICOSIg can bind specifically to ICOSL on mouse immature dendritic cells(DCs) and to explore its biological functions. Methods The binding of ICOSIg to immature DCs was observed by FCM. The cytotoxic effect of ICOSIg on DCs was examined by Annexin Ⅴ/PI, CFSE staining, and CCK-8 kit. thymine incorporation was used to analyze the blocking effect ICOSIg on mixed lymphocyte reaction (MLR). Results Human-derived soluble fusion protein ICOSIg could bind to ICOSL on mouse bone marrow-derived immature DCs and inhibited the MLR, but it neither induced early or late apoptosis of DCs nor affected their proliferation. Conclusion Human-derived ICOSIg constructed in this study has a potent biological function; it has no toxic effect against mouse immature DCs. It is demonstrated that human-derived ICOSIg can sepecifically bind to ICOSL on mouse immature DCs.

Abstract:Objective To investigate the differences in expressions of P-glycoprotein (P-gp), breast cancer drug resistance protein (BCRP) and pulmonary resistance protein (LRP) between primary breast carcinoma and metastatic lymph nodes. Methods The expressions of P-gp, BCRP and LRP in breast cancer tissues, including 126 primary carcinoma and 66 metastasis lymph nodes, were determined immunohistochemically on formalin-fixed, paraffin-embedded tumor sections. Results (1)The positive expression rates of P-gp, BCRP and LRP were 41.27%(52/126), 38.89%(49/126),and 65.87%(83/126) in primary breast carcinoma, and were 59.09% (39/66),63.64%(42/66),and 60.61%(40/66) in metastatic lymph nodes, respectively. The positive rates of P-gp and BCRP in the metastatic lymph nodes were significantly higher than those in primary cancer tissue(P<0.05); there was no significant difference in expression of LRP (P>0.05) .(2) There was a poor consistency in P-gp and BCRP expression between the primary and metastatic sites(P<0.01, Kappa value were 0.276 and 0.356); there was a consistency in LRP expression between the primary and metastatic sites(P>0.05).(3) The co-expression rate of two resistance proteins in primary breast carcinoma was 35.71%(45/126), of three resistance proteins was 15.08%(19/126), and of two or three resistance proteins was 50.79%(64/126), being significantly higher than that of a single protein 33.33% (42/126,P<0.05). The co-expression rate of two resistance proteins in metastasis lymph nodes was 53.03%(35/66), which was significantly higher than that of a single protein 27.27%(18/66,P<0.05). The co-expression rate of three resistance proteins was 16.67%(11/66), two or three resistance proteins was 69.70%(46/66), which was significantly higher than that of a single protein (P<0.01). Both the co-expression levels of two resistance proteins and two or three resistance proteins in metastatic lymph nodes were significantly higher than those in primary cancer (P<0.05) .(4)Kaplan-Meier analyses revealed that patients with P-gp, BCRP and LRP expressed in the metastatic lymph nodes had a lower 5-year survival rate compared with patients whose primary breast cancer being positive for them. Conclusion The expression of P-gp and BCRP is different between the primary and metastatic breast cancer sites, and there is no significant difference in LRP expression. Coordination of multi-protein expression is a major feature of resistance. Metastasis lymph nodes may have a stronger resistance.

Abstract:Objective To investigate the effect of phospholated-ERK1/2 on NF-κB p65 expression in the substania nigra(SN) of 1-methyl-4-phenyl-1,2,3,6-tetrahydropyridine (MPTP)-induced mouse model of Parkinson’s disease(PD) . Methods PD mouse model was induced by MPTP and the behavior of mouse was observed. Immunohistochemistry and Western blotting analysis were used to observe the changes in expression of tyrosine hydroxylase (TH), NF-κB p65 and p-ERK1/2 in the SN of midbrain. Meanwhile, the above changes were also observed after treatment with U0126, a specific inhibitor of ERK. Results 1 h after the third MPTP administration, there were much more p-ERK1/2 positive cells than NF-κB p65 positive cells in the SN. 24 h after the fifth injection of MPTP, NF-κB p65 positive cells were significantly increased and p-ERK1/2 positive cells were decreased, accompanied by marked loss of TH positive neurons. The above changes were greatly alleviated in animals treated with U0126. Conclusion ERK1/2 pathway may regulate NF-κB p65 activation in MPTP-induced mouse model of Parkinson’s disease, which leads to loss of dopamine neurons.

Abstract:Objective To explore the effects of 3'-hydroxygenistein on the proliferation and differentiation of 3T3-L1 preadipocytes and to elucidate the related mechanism. Methods 3T3-L1 preadipocytes were cultured and treated with different dosages of 3'-hydroxygenistein. Cell proliferation was analyzed by counting cell numbers and MTT assay; DNA synthesis of 3T3-L1 was investigated by bromodeoxyuridine (BrdU) incorporation assay; the degree of preadipocytes differentiation was evaluated by Oil red O staining; and the expressions of peroxisome proliferation activated receptor γ (PPARγ) and CAAT/enhancer binding protein (C/EBPα) were detected at mRNA and protein level by real-time PCR and Western blotting analysis, respectively. Results Pretreatment with 3'-hydroxygenistein (10-50 μmol/L) for 24, 48 and 72 h markedly promoted 3T3-L1 preadipocyte proliferation and differentiation in a dose-effect manner (P<0.05, P<0.01). It also significantly facilitated the DNA synthesis in 3T3-L1 preadipocytes in a dose-dependent manner (P<0.05). Similar to rosiglitazone (ROZ), 3'-hydroxygenistein (at 10 or 50 μmol/L) also resulted in more lipid droplets in 3T3-L1 preadipocytes (P<0.05, P<0.01). Furthermore, 3'-hydroxygenistein greatly increased the mRNA and protein expression of PPARγ and C/EBPα in 3T3-L1 preadipocytes (P<0.01). Conclusion 3'-hydroxygenistein can promote the proliferation and DNA synthesis of 3T3-L1 preadipocytes; it can also enhance the accumulation of lipid drops and increase the terminal differentiation of preadipocyts, which might be associated with its ability to increase the expression of PPARγ and C/EBPα.

Abstract:Objective To establish a rapid, reliable method for identifying and typing of Clostridium botulinum using 16S rDNA sequencing and phylogenetic tree construction. Methods Clostridium genome templates were extracted from 9 strains, including LCL063, 830110, LC175, LCL155, 66418, N153, 61082, ALASKA, and IWANAI; 16S rRNA genes were amplifed by PCR with the 16S rRNA specific primers, and then the PCR products were cloned to pGEM -T Easy vector and sequenced. Finally, the sequence of 16S rDNA was analyzed by Clustal and Mega program;the phylogenetic trees were constructed by Neighor joining and Maximum parsimony. Results It was found that LCL063, 830110, and LCL175 were BONT/E producing Clostridium butyricums; IWANAI and ALASKA were Clostridium botulinum type E. The results of the present method were consistent with those of the conventional method. Conclusion 16S rRNA sequencing combined with phylogenetic tree analysis is a rapid and accurate method in Clostridium botulinum identification, and the method may serve as a criterion for bacterial typing with the completion of ribosomal RNA data bank.

Abstract:Objective To investigate the clinical effectiveness and safety of ultrasound-guided percutaneous thermotherapy for benign thyroid nodules. Methods Ultrasound-guided percutaneous radiofrequency or microwave ablation was conducted following tru-cut biopsy of 50 benign thyroid nodules in 40 patients, including those with adenomas and hyperplastic nodules.The necrosis and shrinkage in the ablated areas were evaluated.The adverse effects and complications related to ablation were observed. Results All the 50 thyroid nodules were deprived of blood supply after ablation as shown by contrast-enhanced ultrasound, and the ablated area completely covered the size of the nodules.Follow-up ultrasound examination demonstrated that the ablated areas were shrunk to various degrees, including completely disappearance in 6 (12%), 32(64%) with the diameters reduced by more than a half, and 12 (24%) with the diameters reduced by less than a half.There were no skin burns in the neck, bronchial or esophageal injuries, recurrent laryngeal nerve palsy, or complication related to neck muscle injury. Conclusion Ultrasound-guided percutaneous thermal ablation is a safe, minimally invasive, controllable and definitely effective method for treating benign thyroid nodules.It has a great clinical potential.

Abstract:Objective To analyze the characteristics of surface electrocardiographic (ECG) and intracardiac electrophysiological mapping of atrial premature contractions (APC) and atrial fibrillation (AF) originated from the superior vena cava (SVC), so as to investigate the electrophysiological mechanism of AF with SVC origin. Methods The clinical data of 12 patients(mean age 55.3±12.2 years) with paroxysmal AF were retrospectively analyzed; the patients were electrophysiologically confirmed with AF of SVC origin and were hospitalized for radiofrequency ablation during Sept. 2006 to Aug. 2010. The P waves of APC from SVC in inferior leads and V1, morphology of AF, procedure time, ablation sites within SVC, complications and success rate were all analyzed. Results Compared with P wave of sinus rhythm (SR), the P amplitude of APC from SVC was significantly greater ( mV vs mV, P<0.05) in lead Ⅱ, and in lead V1 the ratio of biphasic morphology was significantly higher (91.6% vs 33.3%, P<0.05). The figures of fibrillation wave were similar to P waves of SR and APC in 8 patients (8/12,66.6%). Compared with the electrical isolation of left superior pulmonary vein (LSPV), SVC isolation needed a significantly shorter time ( min vs min, P<0.05)and a significantly less ablation site ( vs , P<0.05). Eight patients still presented persistent rapid firing within SVC after isolation; two patients suffered paroxysmal atrial flutter 1 month after procedure and were controlled by antiarrhythmia drugs. No patients had complications. Conclusion The APC and AF of SVC origin manifest specific ECG patterns, which helps to predict the target vena before ablation; the isolation of SVC needs a shorter time period and less ablation points, and with high success rate.

Abstract:Objective To search for method for treatment and prevention of urethrovesical anastomotic leakage (PUAL) following laparoscopic radical prostatectomy (LRP). Methods Eight (5.3%) of the 151 laparoscopic radical prostatectomies performed between Jan. 2006 and Jan. 2011 developed PUAL. Running urethrovesical anastomosis was used for 58 patients and traditional suture was used in 93 during LRP. The mean age of the 8 patients was 66 years (ranging 53 to 78 years), the mean preoperative total prostate specific antigen(T-PSA) was 34.3 ng/ml (ranging 1.1 to 165.0 ng/ml). The preoperative Gleason sum was <7 in 4 patients, 7 in 2, and > 7 in 2 patients. The mean preoperative Gleason score was 7 (ranging 5 to 9). Pathological stage was T1c in 5, T2a in 2, and T2c in 1 patient. The mean operation time was 202 min (ranging 60 to 360 min). Several treatments were used, including prolonged retropubic drainage, bladder catheter traction, drain position adjustment, reduction of fluid intake, delayed bladder catheter removal and enhancing nutrition for patients. Patients underwent reoperation using a laparoscopic approach after failure of the initial management. Results PUAL was not observed in LRP cases with running laparoscopic suture technique, which was significantly better than cases with traditional suture method (0 vs 8.6% ,P<0.05). Six patients were cured by conservative treatments, 2 patients required reintervention via a laparoscopic approach. The catheters of all patients were removed after a mean of 33 d (ranging 21-43 d). Urethral stricture was not found during a 39-month follow-up(ranging 22 to 60 months). Conclusion Urethrovesical anastomotic leakage can be effectively avoided by running urethrovesical anastomosis; conservative treatment and laparoscopic repair are effective managements to treat PUAL.

Abstract:Objective To investigate the values of the multi-planar reformation(MPR), volume rendering(VR) and maximum intensity projection(MIP) following multislice computed tomography (MSCT) examination in the diagnosis and therapy of impacted tooth. Methods Ten patients confirmed with impacted tooth by X-ray film were scanned with MSCT and the data were sent to the workstation for reconstruction of the MPR, VR, and MIP. Results MPR was able to demonstrate the relative bone intensity around the implanted tooth and exactly determine the distance of impacted tooth with the buccolingual bone wall or adjacent teeth. VR directly displayed the three-dimensional position of the impacted tooth and its relationship with the adjacent teeth. MIP displayed the anatomic structure of the impacted tooth and the bone density around it. Conclusion Combination of MPR, VR, and MIP can directly and accurately demonstrate the location of the impacted tooth and its anatomic relation with the adjacent teeth, providing reliable information for surgical and orthodontic treatment.

Abstract:Objective To study the effect of negative emotion evocation on the autonomic function activity of hearts in healthy subjects, and to initially discuss the mechanism by which negative emotion is involved in the development of coronary heart disease. Methods We selected 69 healthy university students as our subjects. A computer game was used to induce the stress among them; the skin code, heart rate and heart rate variability (HRV) were collected and compared at different phases. Results The high frequency (HF) value was significantly decreased(F =10.773,P<0.01), the low frequency (LF) value was significantly increased(F =5.620,P<0.01), and LF/HF ratio was significantly increased(F =40.327,P<0.01) at both expecting phase and coping phase, which indicates significant changes of cardiac sympathovagal activity, including the relative excitation of sympathetic nerve, relative depression of the vagus nerve, and aggravated autonomic imbalance. Conclusion Exposure of healthy individual to acute emotional stress can induce autonomic variation similar to that of coronary heart disease, indicating that long-term negative emotional stress may be one of the risk factors for vagal dysfunction.

Abstract:Objective To observe the inflammatory infiltration and morphological changes of lung after treatment with unlinastatin in cholestatic rats, so as to study the protective effect of unlinastatin on the lung of cholestatic rats. Methods Rat obstructive jaundice models were established by bile duct ligation (n=48). Ulinastatin (UTI, 100 000 U/kg) was injected intraperitoneally after operation. The lung tissues were collected at day 1, 3, 7, and 14 after operation. The hepatic function and pulmonary pathology were observed after treatment; dry/wet ratio and water content were calculated. The parameters for alveolar capillary permeability, content of myeloperoxidase (MPO), and activity of malondialdehyde (MDA) were also assessed. Expression of TNF-α and IL-1β was examined by immunohistochemical method. Results The serum bilirubin,dry to wet ratio and water content of the lung tissues were not significantly different between UTI group and jaundice group(P>0.05). The levels of evans blue dyes (EBD), MPO, and the activity of MDA in UTI group were significantly lower than that in the jaundice group and UTI groups at the same time points (P<0.05). The pulmonary histology showed alleviated edema, less neutrophil infiltration and RBC in alveolar space in UTI group compared to jaundice group. The pulmonary expression of and IL-1β and TNF-α was significantly lower in UTI group than that in jaundice group on day 1 and day 14 (P<0.01). Conclusion Ulinastatin can inhibit pulmonary expression of pro-inflammatory cytokines TNF-α and IL-1β and decrease MPO activity and MDA levels in bile duct-ligated rats, thus improving the permeability of the pulmonary capillary membrane in obstructive jaundice rats and decreasing the pulmonary inflammatory reactions.

Abstract:Objective To investigate the preparation method, the release profile and structure of the polyelectrolyte layer-by-layer coated chitosan-alginate microgels. Methods The cores of the microgels were prepared by a high voltage electrostatic system, and the semipermeable membrane outside the microgel was polyelectrolyte deposits on the core surface through electrostatic attraction. The influences of different ratios of materials on the expansion property and the in vitro cumulative release of the coated microgels were evaluated by a single factor experiment. Results The prepared polyelectrolyte-coated microgels were well-shaped, with a narrow range of diameter distribution. The lag time of in vitro release was 2.67 h; the release was rapid after lagging, with the cumulative in vitro release being 72% within 3 h. Conclusion Polyelectrolyte layer-by-layer coated chitosan-alginate microgels can release payload in a pulsed fashion in vitro.

Abstract:Objective To investigate the in vitro and in vivo release of chitosan-alginate microgels coated layer-by-layer by polyelectrolyte self-assembly. Methods The cores of the microgels were made by gelatinization using electrostatic microencapsulated and coated by polyelectrolytes using electrostatic attraction.The effects of different layers and ratios of polymer on the in vitro release of FITC-dextran were evaluated. Histological examination was carried out to observe the in vivo release process by injecting the coated microgels into mice. Results The results showed that alginate and calcium chloride concentrations and polyelectrolyte layers markedly affected the lag time of pulsed release and the releasing speed after lagging. Conclusion The release of microgels coated layer-by-layer by polyelectrolyte can be controlled in vitro and can be observed in vivo; meanwhile, the microgels are safe and have good biocompatibility.

Abstract:Objective To design and synthesize a series of novel matrine derivatives and to investigate their in vitro anti-inflammation activities. Methods Ten novel matrine analogues were synthesized via thiosulfate reaction and classical Michael addition using sophocarpine as the starting material. The effects of all the analogues on TNF-α production and NF-κB transcriptional activity were evaluated in vitro. Results The synthesized compounds were confirmed correct by ¹HNMR and ESI-MS. Biological studies showed that the synthetic derivatives had inhibitory effects against TNF-α production and NF-κB transcriptional activity. Compound 1f had the strongest inhibitory effect against TNF-α production, with an IC₅₀ value of 9.4 μmol/L. Conclusion Introduction of liner small substitutes at the 13-position of matrine can enhance its anti-inflammatory activity.

Abstract:Objective To obtain fully protected glyco amino acid for synthesis of various glycopeptides. Methods With galactose as the initial compound, the full benzyl protected β-D-1-thio-galactopyranoside (5) was obtained after 5 steps of reactions. Then it was used as donor for glycosylation with Fmoc-Ser-OAlly to yield fully protected glyco amino acid. Finally, the allyl group was removed to obtain the objective glyco amino acid (1). Results The structures of the intermediate and objective compounds were confirmed by MS, ¹HNMR and ¹³CNMR, and the target glyco amino acid was obtained. Conclusion The present synthetic method is simple to perform and can be widely used; meanwhile, the protective base can be easily removed. The target glyco amino acid is suitable for synthesis of various glycopeptides.

Abstract:Objective To observe the effects of lipopolysaccharide (LPS) on B cell function and the related signal transduction pathways. Methods Freshly purified splenic B cells (CD19⁺ B) of mice were isolated by magnetic activated cell sorting. After stimulation with LPS, the culture supernatants were collected for immunoglobulin isotyping by cytometric bead array (CBA) technology. Flow cytometry was used to analyze costimulatory molecule expression and culture supernatants were also collected for measuring IL-6, IL-10, IL-12p70 and TNF by CBA technology. PD98059 (ERK inhibitor), SP600125 (JNK inhibitor), SB203580 (p38 MAPK inhibitor) and PDTC (NF-κB inhibitor) were used to study the signal pathways for the induced secretion of IL-6, IL-10 and TNF in B cells. Results LPS induced IgG₁ -κ and IgM-κ production, up-regulated the expression of CD40, CD80, CD86 and MHC class Ⅱ, and promoted IL-6, IL-10 and TNF secretion by B cells. Furthermore, JNK, p38 MAPK, and NF-κB pathways regulated the secretion of IL-6, IL-10 and TNF in B cells. Conclusion LPS can regulate B cell function by inducing Ig production, cytokine secretion, and expression of costimulatory molecules.

Abstract:Objective To study the effects of scutellarin on LPS-induced pro-inflammatory cytokine expression in BV2 cells, so as to explore the anti-inflammatory mechanism of scutellarin. Methods The experiment was divided into 5 groups, namely, a normal control group, a model control group (stimulated by LPS), and three Scutellarin treatment groups (0.1, 1, and 100 μg/ml). NF-κB activity was examined by Luciferase Reporter Gene Assay. BV2 cells were treated with LPS ; expressions of TNF-α and IL-1β mRNA and protein were examined by real-time PCR and Western blotting analysis, respectively. Results Scutellarin treatment greatly inhibited LPS-induced NF-κB activation in pNF-κB 293 cells. The treatment also noticeably inhibited LPS-induced expression of TNF-α and IL-1β in BV-2 cells. Conclusion Scutellarin has anti-inflammatory effect, which maybe related to the inhibition of NF-κB-mediated pathway and inhibition of pro-inflammatory cytokine expression.

Abstract:In recent years, researchers have discovered that NADH and NADPH have important functions in physiological and pathological conditions. In this paper we discussed the synthesis and degradation of NADH and NADPH, focused on the function of NADH, NADPH and NADPH oxidase, and described their functions in various pathological conditions such as inflammation, cardiovascular disease, cancer, neurodegenerative diseases. Nowadays, the functions and metabolism of NADH (NADPH) have evoked international research interest; and the underlying mechanism will be better understood.

Abstract:Genetic susceptibility exists in intervertebral disc degeneration(IDD) diseases,which may be associated with gene polymorphisms.Recently,much progress has been made in the relationship between IDD susceptibility and gene single nucleotide polymorphism(SNP). The genes associated with IDD mainly include 3 types: genes associated with intervertebral disc stability (about structure and metabolism), genes associated with inflammation, and genes associated with pain signal channel. Studies of SNP in genes associated with IDD can help to identify its molecular mechanism, paving a way for individualized prevention and treatment of IDD.

Abstract:Breast tumors are the most common tumors of epithelial origin. Some tumors or tumor-like lesions of the breast may display a morphology similar to mesenchymal tumors predominated by spindle cells. However, such morphology is apt to be confused with others due to lack of the characteristic histopathology. This paper reviews some spindle cell lesions in the breast, in an attempt to provide theoretical evidences for the differentiation diagnosis of breast tumors and tumor-like lesions.

Abstract:Objective To explore the application of magnetic resonance angiography and virtual reality technique in pre-operative planning for sphenoid ridge meningioma. Methods Ten patients with sphenoid ridge meningiomas were examined by brain CT, MRI and MRA before operation. Then the image data were transferred to the Dextroscope system. The images were reconstructed using the Radiodexter software to display the three dimensional image. The tumor and its adjacent anatomic structures could be viewed and the system tools allowed for simulation of the operation, so the full information of the tumor, basilar texture, and nerves and vessels could be obtained. A pre-operative planning was done and was compared with intra-operation situation. Results The virtual reality images of the 10 cases were successfully conducted, and all the procedures were performed according to the pre-operative planning, with a consistent rate of 100%. Radical resection was achieved in all the 10 patients and there were no sequelea. Conclusion Magnetic resonance angiography and the virtual reality system can provide the information of tumor and its adjacent structures, so it can help the neurosurgeons in planning the details for operation, contributing to operation safety and tumor resection.

Abstract:目的 采用重组慢病毒CIAPIN1表达载体和CIAPIN1沉默载体感染食管癌EC9706细胞,观察调控CIAPIN1基因表达对细胞增殖、细胞周期、细胞凋亡及体外侵袭转移的影响。 方法 构建CIAPIN1表达慢病毒载体和CIAPIN1 siRNA慢病毒载体,包装得到重组慢病毒;分别感染食管癌EC9706细胞,筛选得到稳定CIAPIN1基因高表达和低表达的细胞。实验分为CIAPIN1表达组、CIAPIN1 siRNA组、无关siRNA对照组和空白对照组。采用RT-PCR和蛋白质印迹法分析CIAPIN1基因和蛋白的表达,MTT实验检测细胞增殖,流式细胞术(FCM)分析细胞周期及细胞凋亡的变化, Boyden小室检测细胞侵袭转移。 结果 与空白对照组、无关siRNA对照组和CIAPIN1 siRNA组比较,CIAPIN1高表达组细胞生长受到抑制(P<0.05);S和G₂/M期细胞比例减少,G₀/G₁期细胞比例增加(P<0.05);平均细胞凋亡率增加(P<0.05),穿透Matrigel的平均细胞数减少(P<0.05)。 结论 以慢病毒为载体介导的CIAPIN1基因高表达可有效抑制食管癌EC9706细胞增殖、促进细胞凋亡和降低细胞侵袭迁移能力。

Abstract:目的 通过实时测定单态氧(singlet oxygen,¹O₂)评估635 nm和(或)670 nm激光照射下盐酸氨基乙酰丙酸(5-aminolevulinic acid,5-ALA)介导的光动力学治疗(photodynamic therapy, PDT)抗肿瘤效果。 方法 以5-ALA为光敏剂,在单波长(635 nm或670 nm)及联合波长(635 nm+670 nm)下进行激光照射,利用新型高灵敏的检测系统测定PpIX溶液、肿瘤细胞(9L神经胶质肉瘤细胞和HeLa细胞)及HeLa负瘤裸鼠中的¹O₂强度,H-E染色法检测肿瘤组织坏死深度。 结果 接受单一的635 nm或670 nm波长激光照射时,PpIX溶液¹O₂强度在两者中无明显区别。635 nm与670 nm激光联合照射下,9L细胞的¹O₂强度高于HeLa细胞;在两种细胞中670 nm激光诱发¹O₂强度均远远低于635 nm激光。在HeLa细胞裸鼠皮下移植瘤中,两种波长激光联合照射产生一定的肿瘤坏死深度,但低于635 nm单波长照射。 结论 在5-ALA介导的PDT中,670 nm波长激光显示了一定的PDT疗效。

Abstract:目的 总结1例累及右心的子宫静脉内平滑肌瘤病的临床诊治经验,并结合文献资料进行讨论,以利于提高对此罕见病的诊治水平。 方法 患者,女性,38岁,因"发热、下腹胀痛、胸闷1周"于2010年7月入院。患者G1P0,2002年因"子宫肌瘤"行"子宫肌瘤剥除术",2003年12月因"子宫肌瘤复发"行"子宫全切除术"。因强烈要求生育,拟行代孕,患者于2010年7月在美国接受注射促卵泡药物"丽申宝注射液",每日3支共计12 d(剂量不详),后因发热、腹胀、胸闷自感症状加重不能承受,即回国就诊。入院后行超声、CT、MRI等影像检查以明确诊断。 结果 检查提示:下腹部及盆腔巨大囊实性占位性病变,左肾积水、腹腔积液,右心房、下腔静脉、左肾静脉、双侧髂静脉瘤栓形成。予行胸腹联合手术,切除右心、下腔静脉等瘤栓,切除盆腔肿瘤,病理诊断为子宫静脉内平滑肌瘤病。 结论 子宫静脉内平滑肌瘤病属于少见病,累及心脏更罕见,近期发生增多可能与辅助生殖中性激素使用频次增加有关;影像学检查可辅助诊断,最终确诊依赖病理;手术切除是首选治疗方式,须切除彻底,防止复发。

Abstract:目的 总结50例右侧乳房下小切口微创行左房室瓣手术的临床经验和疗效。 方法 采用右侧乳房下小切口结合头灯光源辅助方法共对50例左房室瓣疾病患者实施体外循环左房室瓣置换或成形手术(微创组),所有患者采用单腔气管插管,仰卧位,右侧垫高20~30°,右上肢悬吊,插股动脉供血管、双极股静脉引流管的插管技术实施周围体外循环。手术主要切口为右侧乳房下小切口6~8 cm(经该切口第4肋间进胸可进行心脏操作)+两孔(右侧锁骨中线第2肋间放置长冷灌针头和牵引上腔静脉阻断带、右侧腋中线第4肋间放置特制的升主动脉阻断钳和牵引下腔静脉阻断带),术者佩戴头灯,经切口可提供良好的视野光源。经房间隔切口或房间沟切口进行左房室瓣手术。对照组为同期传统正中开胸左房室瓣手术的患者50例。 结果 微创组无中转开胸、延期拔除气管插管、重要脏器功能衰竭、血红蛋白尿及手术死亡病例,4例术中低潮高频通气出现低氧血症,处理后好转,1例术后因肋间肌肉血管出血再次原切口进胸止血。微创组与对照组相比,手术时间、体外循环时间、主动脉阻断时间、体外辅助时间、术后拔除气管插管时间差异无统计学意义;而微创组术后住院时间缩短,术后胸液引流量、住院期间输血量明显减少(P<0.05)。出院前复查心脏超声示左房室瓣瓣膜位置、活动良好,无瓣周漏,左房室瓣成形术后微量或轻度反流,右房室瓣轻度反流,成形效果满意。 结论 右侧乳房下小切口第4肋进胸结合术者头灯光源辅助、周围体外循环的方法行微创左房室瓣手术,手术安全,术野暴露良好、操作简便易掌握,较常规正中开胸手术创伤小、出血少、住院时间短、切口美观隐蔽,术后早期效果理想。

Abstract:目的：探讨子宫动脉栓塞术在中期妊娠前置胎盘状态引产中的应用。方法与结果: 报道一例应用子宫动脉栓塞术治疗中期妊娠前置胎盘状态引产出血病例。患者31岁，G5P3，孕25+2周合并中央性前置胎盘,行米非司酮口服+利凡诺羊膜腔内注射引产后4天，多量阴道流血入院。入院后给予经导管子宫动脉栓塞术，术后5小时，胎盘胎膜包绕胎儿同时娩出，产时产后出血少。 结论： 子宫动脉栓塞术能有效协助中期妊娠合并胎盘前置状态引产，微创且止血确切，避免开腹手术，值得进一步探讨。

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