Abstract:ObjectiveTo determine the 16S rDNA sequences of 36 strains of Halomonas sp. isolated from the seawater of the East China Sea and to study their bioactivities. MethodsThe 16S rDNA sequences of all isolated strains were amplified by PCR, and the obtained se

Abstract:ObjectiveTo generate brevican knockout mutant mice. MethodsThe targeting construct to inactivate the brevican gene was made by using the ET cloning technology. The construct was linearized and electroporated into embryonic stem (ES) cells. Homologous reco

Abstract:ObjectiveTo determine whether the single nucleotide polymorphism (SNPs) on chromosome 9p21 and 1p13 were associated with acute myocardial infarction(AMI) in a Chinese population, and to compare the allelic frequencies of risk SNP between populations livin

Abstract:ObjectiveTo elucidate the relationship between hepatic histopathologic progression and findings of clinical noninvasive examination in patients with non-viral liver diseases. MethodsA total of 222 patients with non-viral liver diseases, who received liver biopsy in our hospital, were included in the present study. The association of different histopathological progression (grade of hepatic inflammation and stage of fibrosis) with the noninvasive examination parameters (serum biochemical indicators and imaging findings) was investigated. ResultsWe found that age,serum biochemical indicators, platelet (PLT), and ratios of aspartate aminotransferase to platelet (AST/PLT), aspartate aminotransferase to alanine aminotransferase (AST/ALT) and albumin to globulin (A/G) were significantly associated with the histopathological grades and stages of hepatic tissues. The grades of inflammation were markedly different between 2 groups with ALT levels higher or lower than 1 upper limits of normal (ULN) (P=0.009); the stages of hepatic fibrosis were significantly different between 2 groups with ALT levels higher or lower than 2 ULN (P=0.030). The result of B-type ultrasonic examination was positively related to the grade of hepatic inflammation and stage of fibrosis (r=0.417,P=0.000；r=0.530,P=0.000). AST/PLT and prothrombin time (PT) level were significantly different between patients with hepatic fibrosis and those without (P=0.015, P=0.000); AST/ALT, AST/PLT, A/G and PT levels were also significantly different between cirrhotic patients and non cirrhotic ones (P=0.042,0.012,0.000,and 0.003, respectively). The area under ROC curve of noninvasive analysis model APRI was the largest one among the 3 models. APRI model had the highest sensitivity in diagnosing liver fibrosis, but with a poor specificity; S-Index had a higher specificity but a poor sensitivity. ConclusionClinical noninvasive parameters are valuable for evaluating the activity of hepatic inflammation and stage of fibrosis in patients with non-viral liver diseases. Noninvasive analysis model is useful for diagnosing liver fibrosis and early cirrhosis.

Abstract:ObjectiveTo observe the influence of circumferential pulmonary vein isolation (CPVI) on P-wave polarity and amplitude and to investigate whether the changes of P-wave are associated with patient outcomes after initial CPVI. MethodsFifty patients with paro

Abstract:ObjectiveTo establish mouse models of total body irradiation (TBI) with different doses of 60Co γ radiation combined with skin defect, so as to investigate the influence of TBI on wound healing and the pathophysiological changes in combined radiation inju

Abstract:ObjectiveTo establish a finite element model of gunshot wound on the maxillofacial region in human, and to conduct dynamic simulation and analysis of the injury process and degree of gunshot wounds to the center of mandible mental region, so as to explore

Abstract:ObjectiveTo investigate the expression of inflammatory factors in myocardium following coronary microembolization (CME) and effect of NF-κB inhibitor on the expression. MethodsCME models were created in 64 rats by injecting homologous microthrombotic part

Abstract:ObjectiveTo screen for the serum proteomic patterns and related genes in early liver metastasis of colorectal cancer by surface enhanced laser desorption/ionization time of flight mass spectrometry (SELDI-TOF-MS) and the RT2 ProfilerTM PCR Array Human Tum

Abstract:ObjectiveTo evaluate the inhibitory effects of recombinant adenovirus carrying TEM8 (Ad-TEM8) against angiogenesis and growth of mouse glioma G422 cells in vitro and in vivo. MethodsThe Ad-TEM8 was constructed and subcutaneously injected to immunize Kunmi

Abstract:ObjectiveTo study the expression of intermediate-conductance-Ca2＋ -activated K＋(KCa3.1) channels in endometrial cancer tissues and their role in regulating cell cycle of endometrial cancer cells. MethodsReal-time PCR and Western blotting analysis were use

Abstract:ObjectiveTo observe the effect of ulinastatin on the survival and lymphocyte apoptosis in mice with cecal ligation and puncture(CLP) induced sepsis, so as to investigate the possible mechanism of ulinastatin action in sepsis. MethodsForty 8-10 weeks old m

Abstract:ObjectiveTo evaluate the protective effect of curcumin against reperfusion injury of mouse renal tubular epithelial cells in vitro and the related mechanisms. MethodsThe renal tubular epithelial cells (TECs) were isolated by an improved Percoll density gr

Abstract:ObjectiveTo explore the effect of anshinoneⅡA(Tan ⅡA) on induction of apoptosis in human nasopharyngeal carcinoma CNE1 cells and on expression of Bcl-2 gene and COX-2 promoter. MethodsCNE1 cells were cultured in vitro and treated with various concentratio

Abstract:ObjectiveTo explore the relationship between emotional change and hormonal levels in female college students during the menstrual cycle.MethodsMenstrual distress questionnaire(MDQ) was used for emotional evaluation. Hormone samples were collected from sal

Abstract:Objective: To investigate the protective mechanism of spironolactone which pretreatmented in period of percataneous coronary interventional(PCI),by detecting the variation of mean arterial pressurein root of aorta（MAPA）, concentrations of aldosterone, Troponin I（cTn-I）, brain natriuretic peptide（BNP）and c-reaction protein(CRP). Methods: Patients who need to PCI in department of cardiology of Rugao people’s hospital from Junly,2007 to December,2010 were divided into two groups randomly, group of therapy (A group, n=116),group of contrast(B group, n=121).Spironolactone（20-40mg/d） was used in patients in group A ,and placebo was given to patients in group B. The levels of MAPA and the concentrations of Aldo,cTn-I,BNP ,CRP before and after PCI were detected or measured. All datas was analysed by software of SPSS11.5. Results: There were no significant different in age, sexual, operation time, times of balloon and number of stents between two groups. The concentrations of Aldo，cTn-I，BNP and CRP were all raised up in two groups after operation; there were no great difference in the concentration of the Aldo between two groups, but concentrations of cTn-I、CRP、BNP in group A were much lower than that in group B（P<0.05） and the level of MAPA was higher than that in group B. There were obvious dependablity between ΔMAPA(postoperative MAPA -preoperative MAPA)andΔcTn-I(postoperative cTn-I / preoperative cTn-I),ΔCRP(postoperative CRP/ preoperative CRP),ΔBNP(postoperative BNP/ preoperative BNP), and coefficient correlations were 0.61、0.38、0.59(P<0．05orP<0.01) Conclusion: Spironolactone could alleviate the injury of myocardial damage,and lessen inflammatory reaction in preoperative period of PCI. The possible pathomechanism might be related with that it could encourage the non-genomic effects of Aldo, and improve the perfusion of coronary.

Abstract:Objective To observe the therapeutic effect of bismuth potassium citrate tablets (BPCTs) on gastritis and gastric ulcer. MethodsUsing rat foot swelling, cotton pellet granuloma test, mouse writhing test and hot plate test, we observed the anti-inflammatio

Abstract:Glycosaminoglycans (GAGs) are a group of important large biological molecules containing a hexosamine and an uronic acid component. Glycosaminoglycans-binding cytokines, such as vascular endothelial growth factor, midkine, osteopontin, heparanase, and bas

Abstract:Multiple sclerosis (MS) is an autoimmune disease involving the central nervous system. T lymphocytes are known to play an important role in maintaining the body’s autoimmunity, and their dysfunction is the leading cause of MS. Recent study has found that

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Abstract:Folic acid, an essential microelement to human, is closely related to tumor development and progression. Recent studies have suggested that a low folate intake and impaired folate metabolism may be involved in the carcinogenesis of pancreatic cancer, but

Abstract:This paper reviews the composition, mechanisms, safety and clinical application of major absorbable hemostatic materials, including oxidized cellulose, oxidized regenerated cellulose, thrombin sealants, fibrin glue, collagen, gelatin, α-cyanoacrylate seal

Abstract:目的探讨合并门静脉高压症(PHT)的肝细胞肝癌(HCC)患者同期联合手术的可行性。方法回顾性总结分析161例同期联合手术治疗HCC合并PHT患者的临床资料。结果术后死亡1例(0.62%),总体并发症发生率55.3%。联合脾切除+贲门周围血管离断组和联合脾切除组术后肝功能损害及腹水发生率等两两比较差异无统计学意义。联合脾切除+贲门周围血管离断组术后有腹水者术前前白蛋白(PA)值为(120±19) mg/L,低于无腹水者(172±34)mg/L,差异有统计学意义(P<0.01)；肿瘤直径>5 cm者联合手术后

Abstract:目的研究冠心病患者血清γ-谷氨酰转移酶(GGT)与冠状动脉（冠脉）粥样硬化病变的严重程度、冠脉病变的稳定性及冠心病主要危险因素的关系。方法依据冠脉造影检查确诊为冠心病的患者132例，按病变严重程度分为单支、双支、三支病变组，根据临床类型分为稳定性心绞痛(SAP)、急性冠脉综合征(ACS)组，并以冠脉造影检查排除冠心病者30例为对照组。所有患者均检测血清GGT、白细胞计数（WBC）、总胆红素（TBiL）、空腹血糖（FPG）、总胆固醇（TC）、三酰甘油（TG）、高密度脂蛋白(HDL)、低密度脂蛋白（LDL）、

Abstract:目的探讨实施供精人工授精前炔雌醇环丙孕酮片(达英-35)预处理对提高多囊卵巢综合征(PCOS)患者妊娠成功率的作用。 方法100例PCOS患者随机分成两组：(1)治疗组(44例),先口服达英-35共3个月, 在下一个治疗周期采用克罗米芬加绝经期尿促性素(HMG)促排卵,在排卵前48 h内和排卵后12 h内,进行供精人工授精;(2)对照组(56例),在治疗周期直接采用克罗米芬加尿促性素促排卵,同法行供精人工授精。通过治疗前后内分泌激素值、子宫内膜厚度、HMG用量及每周期平均促卵泡数、供精人工授精结局评价达英-35预处理后人工授精的效果。结果治疗组使用达英-35后的血促黄体生成素(LH)浓度、LH／卵泡刺激素(FSH)比值明显较治疗前降低,并且低于对照组,差异有统计学意义(P<0.05)；治疗组促卵泡数、取消周期数、卵泡过度刺激征(OHSS)发生率低于对照组(P<0.01),临床妊娠率高于对照组,流产率低于对照组(P<0.05)。结论PCOS患者行供精人工授精前使用达英-35预处理,不仅增加了促排卵药物的敏感性,提高了卵泡质量,使人工授精的妊娠成功率明显增加,而且降低了OHSS的发生风险,减少了取消的周期数,减轻了患者负担。

Abstract:目的观察成分输血治疗剖宫产术中弥漫性血管内凝血(DIC)的疗效。方法参照DIC诊断标准、治疗原则及成分输血方案制订详细的孕产妇DIC治疗方案,在去除病因的基础上,A组输血内容以补充红细胞悬液、新鲜冰冻血浆及纤维蛋白原,临床纠正失血性休克、及时去除诱发DIC病因等综合治疗；B组输血内容在A组治疗的基础上补充单采血小板、冷沉淀,临床纠正失血性休克、及时去除诱发DIC病因等综合治疗。分析两组输血治疗前后凝血酶原时间(PT)、部分凝血活酶时间(APTT)、纤维蛋白原定量(FGB)、血小板计数(PLT)、凝血酶凝固

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