Abstract:ObjectiveTo investigate the regulatory effect of protein kinase A (PKA) and protein kinase C (PKC) on the function of ATP receptor P2X3 in cultured dorsal root ganglion (DRG) neurons. MethodsRat DRG neurons were collected, cultured and subjected to exogenous ATP treatment to induce transient inward current. Whole-cell patch clamp recording was employed to observe the effect of TNP-ATP (P2X3 and P2X2/3 receptor antagonist) on the induced current. Meanwhile, the regulatory effects of PKA and PKC agonist on ATP-induced transient inward current were also observed. ResultsTNP-ATP inhibited ATP-induced transient current in cultured DRG neurons in a dose-dependent manner, with the IC50 value being (21.7±7.6) nmol/L. PKA agonist forskolin (1 μmol/L) and PKC agonist PMA (1 μmol/L) inhibited ATP-induced transient current rapidly and reversibly. ConclusionPKA and PKC can inhibit ATP-induced transient inward current in cultured DRG neurons, probably through inhibiting the function of P2X3 receptors. It indicates that P2X3 receptor regulation by protein kinases may participate in the development of pain.

Abstract:ObjectiveTo study the therapeutic effect of estrogenic equol (Eq) on mustard gas-caused skin wounds and the possible mechanism. MethodsA rabbit skin wound model was established by contaminating the hip skin with mustard gas (0.5 mg/cm2). The contaminated wound was treated externally with different concentrations (10, 50, and 100 μmol/L) of Eq for a week, and the changes of the wound were observed macroscopically. Wound specimens were collected for histopathological evaluation. Cell apoptosis and DNA cycle changes were analyzed by flow cytometry. Levels of IL-6 and TNF-α in the contaminated wound specimens were detected. ResultsThe scores of erythema, edema, erosion and necrosis of the contaminated wounds in the treatment groups were significantly lower than those in the control group 120 h after mustard gas exposure (P<0.05). The scores for changes of histopathological inflammation and necrosis were significantly lower in the 50 μmol/L Eq treatment group than those in the control group 168 h after mustard gas exposure (P<0.05). The results of flow cytometry showed that the proportion of apoptotic cells in the control group was significantly higher than those in the treatment groups 168 h after mustard gas exposure (P<0.05), and the proportion of cells in DNA (S+G2-M) phase in the treatment groups was significantly higher than that in the control group (P<0.05). The levels of IL-6 and TNF-α in the treatment groups were significantly lower than those in the control group 168 h after mustard gas exposure (P<0.05). ConclusionEq can promote healing of mustard gas-caused local skin damaged by inhibiting inflammatory response and cell apoptosis and enhancing cell proliferation.

Abstract:ObjectiveTo investigate the effect of dexamethasone on polyinosinic:polycytidylic acid (PIC)-induced chemotactic factor expression in human bronchial epithelial (16hBE) cells and the underlying mechanism. Methods16hBE cells were treated with different concentrations of PIC (0.001, 0.01, 0.1, and 1 μg/ml) and dexamethasone (0.1, 1, and 10 μmol/L). IL-8 and IP-10 mRNA levels were detected by RT-PCR 6 h after stimulation. IL-8 and IP-10 protein levels in the culture supernatant were detected by ELISA 24 h after stimulation. NF-κB p65 subunit expression was detected by immunohistochemical staining. ResultsPIC concentration-dependently (0.001, 0.01, and 0.1 μg/ml) increased the expression of IL-8 and IP-10 mRNA and protein compared with the control group, with significant differences found when PIC at 0.01 μg/ml and 0.1 μg/ml (P<0.05,P<0.01). When the concentration of PIC was 1 μg/ml, the expressions of IL-8 and IP-10 were decreased at both mRNA and protein levels. Pretreatment with dexamethasone (1 μmol/L and 10 μmol/L) for 0.5 h significantly inhibited the IL-8 and IP-10 expression at both mRNA and protein levels (P<0.05, P<0.01). Dexamethasone pretreatment (1 μmol/L) significantly inhibited PIC-induced NF-κB p65 subunit expression (P<0.01). ConclusionGlucocorticoids can suppress PIC-induced IL-8 and IP-10 expression in human bronchial epithelial cells, probably through activation of NF-κB pathway.

Abstract:ObjectiveTo establish rabbit model of pulmonary Pseudomonas aeruginosa (PA) infection by two different methods and make evaluation and comparison from the pathology, imaging and other aspects. MethodsTotally 48 healthy New Zealand rabbits were randomly divided into 4 groups (n=12): group A, B, C and D. Group A was inoculated with PA suspension via percutaneous tracheal puncturing; Group B was inoculated with PA suspension via spraying inhaling; and group C and D were treated with sterile saline suspension serving as puncturing control and inhaling control, respectively. PA inoculation was done in the four groups every other day, and chest CT scan was performed every other day after inoculation. Pulmonary pathological examination was done after the death of the animals. The two methods were evaluated and compared concerning the pathological, imaging, and laboratory findings. Results(1) Pathological findings in group A and B: For animals of early death, there were pulmonary edema of different degrees, hemorrhage, nodule-like abscess, and consolidation; moreover, severe neutrophil infiltration and abscess-like lesions in lung tissue were observed under the microscope. For animals of later death, the consolidation was gradually relieved and the main pathological findings included local atelectasis, fibrosis, granuloma formation, and lymphocyte infiltration. (2) Radiographic findings in group A and B: Chest CT showed bilateral multiple patchy opacities, with consolidation and abscess seen in some areas. Lesions on CT were found at 5 days after inoculation in group A and at 7 days after inoculation in group B.ConclusionRepeatedly inoculating PA suspension via both percutaneous tracheal puncturing and spraying inhaling can successfully establish rabbit model of pulmonary PA infection, and each method has their own advantages in performance, controllability, and adaptability.

Abstract:Objective To make the horizontal and vertical mechanical evaluation for different access devices (Port) in transumbilical laparoscopic surgery, and to compare the maneuverabilities of these devices during the process. MethodsA laparoscopic training box was combined with Multi-ports, TriPort and SILS Port to construct the evaluation platforms. A universal testing machine was used for evaluating the mechanics of the three devices on horizontal and vertical direction by traction experiments. ResultsIn the horizontal traction experiment, the forces were significantly different on the instruments with different access devices (Multi-ports

Abstract:ObjectiveTo design a partially bioabsorbable interbody fusion cage (PBIFC) and to analyze its biomechanics using finite-element evaluation method. MethodsA new type of PBIFC was designed and made from nano-hydroxyapatite/polyamide 66 (n-HA/PA66) and multi-(amino acid) copolymer-calcium sulfate; a 3D finite-element model of L3-L4 segment was constructed and validated. A PBIFC or a non-absorbable cage of identical shape (n-HA/PA66 cage) was implanted via anterior approach on the model, and four models were established, including the immediate implantation model and 4-week implantation model of each cage. An axial compressive preload of 400 N and a torque of 10 Nm were applied to the L3 segment to simulate spinal compression, flexion, extension, rotation, and lateral bending. The stress and stress contour of different loading conditions were calculated. ResultsImmediately after implantation, stresses of the bone graft in PBIFC model were higher than those in n-HA/PA66 cage model, while stresses of the cage and endplate in PBIFC model were lower; and no significant difference in stress contours on endplate was found between the two models. Four weeks after implantation, stresses of the bone graft in PBIFC were higher than those in n-HA/PA66 cage, and stresses of the cage and endplate in PBIFC model were lower, with the stress differences being greater than those of immediately after implantation. The stress contours on endplate in PBIFC model was larger than that in the n-HA/PA66 cage model. ConclusionPBIFC is probably more suitable than a non-absorbable cage of identical shape for lumbar interbody fusion.

Abstract:ObjectiveTo investigate ZEB-1 protein expression in hepatocellular carcinoma (HCC) tissues and the relevant significance. MethodsImmunohistochemical method and Western blotting analysis were used to detect the ZEB-1 protein expression in 62 HCC tissues,48 paracancerous cirrhotic tissues and 10 normal liver tissues. The relationship between ZEB-1 protein expression and clinicopathological features of HCC was evaluated. ResultsImmunohistochemical staining showed that the positive rates of ZEB-1 protein expression in HCC tissues and paracancerous cirrhotic tissues were 93.5% (58/62) and 83.3 (40/48), respectively. Western blotting analysis showed that ZEB-1 protein expression in HCC tissues was significantly higher than those in the paracancerous cirrhotic tissues and normal liver tissues (P<0.05). We also found that ZEB-1 expression was significantly correlated with the clinical stage, tumor differentiation degree, metastasis, portal vein tumor thrombus and postoperative recurrence (P<0.05), and was not correlated with the tumor diameter, number of tumors, or serum AFP levels (P>0.05). ConclusionHigh expression of ZEB-1 is correlated with the development and progression of HCC. ZEB-1 might also participate in the epithelial-mesenchymal transition of HCC cells, subsequently contributing to the invasion and metastasis.

Abstract:ObjectiveTo observe the changes in expressions of apoptosis-promoting gene Bax, apoptosis-inhibiting gene Bcl-2, and cytochrome C in the renal tissue of diabetic rats. MethodsTwenty-four male Sprague-Dawley rats were randomly divided into 2 groups (n=12): normal control group and diabetic group. Diabetic models were induced by single intraperitoneal injection of 2% streptozotocin (dissolved in pH 4.4,0.1 mol/L citric acid sodium buffer, 65 mg/kg). Normal control group was only injected with same volume of folic buffer. Animals were sacrificed at the 4th and 12th week, and body mass, 24-hour urine protein, blood glucose, blood urine nitrogen and serum creatinine were determined. The changes of the renal morphology were observed by H-E staining. Immunohistochemical method was used to investigate the expressions of Bax, Bcl-2 and cytochrome C protein. The apoptosis of renal cortex cells was determined by TUNEL method. ResultsCompared with normal control group, the 24-hour urine protein, blood glucose, blood urine nitrogen and serum creatinine were significantly increased in the diabetic group (P<0.05, P<0.01). The size of glomerulus was increased in diabetic rats during the 4th week; hyperplasia of renal glomerulus mesangial matrix, glomerular sclerosis, and vacuolar degeneration in renal tubular epithelial cells were observed during the 12th week. With disease progression in the diabetic group, the expressions of Bax and cytochrome C were increased and the expression of Bcl-2 was decreased. Apoptosis tests showed increased apoptotic cells in the 4th week, mostly in both the distant tubular epithelial cells; in the 12th week, apoptotic cells were seen in both the distant tubular and proximal tubules. ConclusionRenal expression of Bax and cytochrome C gradually increases with the progression of diabetes, inducing apoptosis of more cells and leading to renal dysfunction, which may partly contribute to the diabetic nephropathy in diabetic rats.

Abstract:ObjectiveTo investigate the effect of down-regulated S100A7 expression on proliferation and migration of gastric carcinoma SGC-7901 cells, and to explore the possible molecular mechanisms. MethodsThe experiment was divided into three groups, including untransfected group, control siRNA group and S100A7 siRNA group. S100A7 siRNA and control siRNA were transfected into gastric carcinoma SGC-7901 cells in S100A7 siRNA group and control siRNA group, respectively. Expressions of S100A7 mRNA and protein in gastric carcinoma SGC-7901 cells were examined by Real-time PCR and Western blotting analysis, respectively. CCK-8 kit and Boyden chamber were used to observe the proliferation and migration of SGC-7901 cells in the three groups. The expressions of cyclin D1, Cdk2 and MMP-2 proteins were also examined by Western blotting analysis in the three groups. ResultsS100A7 siRNA effectively down-regulated the expressions of S100A7 mRNA and protein in gastric carcinoma SGC-7901 cells. Down-regulated S100A7 expression greatly inhibited the proliferation and migration of gastric carcinoma SGC-7901 cells, and also down-regulated expression of cyclin D1, Cdk2 and MMP-2 proteins. ConclusionDown-regulated S100A7 expression-mediated inhibition of gastric carcinoma cell proliferation and migration may be closely associated with the decreased expressions of cyclin D1, Cdk2 and MMP-2 proteins.

Abstract:ObjectiveTo investigate the effect of tripterine on surface ultrastructure and proliferative activity of Burkitt lymphoma cell line Raji. MethodsRaji cells were treated with different concentrations (0.5,1.0,1.5,and 2.0 μg/ml) of tripterine. Then the proliferation of Raji cells was determined by CCK8 assay, the cell membrane ultrastructure was analyzed by atomic force microscope, the apoptosis of cells was determined by Hoechst 33258 staining and flow cytometric analysis, and the cell cycle was assayed by flow cytometry. ResultsCCK8 assay showed that the survival rate of cells decreased from (80.67±2.08)% to (38.53±2.25)% 24 h after treatment with different concentrations of tripterine. The cell survival rate decreased from (74.17±3.20)% to (33.22±1.64)% 48 h after treatment. Tripterine significantly inhibited the proliferation of Raji cells and the inhibition was in a time- and concentration-dependent manner. Atomic force microscope scan showed that the untreated Raji cells were round, with relatively smooth surface. After Raji cells were treated with 2.0 μg/ml tripterine for 24 h and 48 h, the ultrastructure of the cell membrane was collapsed, and the cell surface was rough and uneven. Hoechst 33258 staining demonstrated apoptotic cells. Apoptotic rate of the Raji cells increased from (3.50±1.73)% to (38.27±6.05)% 24 h after treatment with different concentrations of tripterine. Flow cytometry analysis showed that 24 h after treatment with 1.5 μg/ml tripterine, S phase Raji cells were significantly increased compared with control group (P<0.05). ConclusionOur results demonstrate that tripterine can alter the cell membrane ultrastructure of Raji cells and can inhibit Raji cell proliferation through inducing cell apoptosis.

Abstract:ObjectiveTo investigate the association of the single nucleotide polymorphisms (SNPs) in exon 4 of ZNF804A gene with schizophrenia in a family-trios sample. MethodsMassARRAY genotyping technique and the transmission disequilibrium test (TDT) were employed to study the association of SNPs (seven nonsynonymous and two intronic) encompassing exon 4 of ZNF804A gene with schizophrenia in 69 nuclear families. Results(1) Four SNPs (rs79776875, rs78816540, rs79082132 and rs62198467) in this study were found to be rare variants (0

Abstract:ObjectiveTo investigate the clinical application of silver-containing biological dressing in covering transplanted meshed autografts for treatment of open wounds (burns and trauma) by comparing with traditional petrolatum gauge on meshed autografts. MethodsEighteen patients fulfilling the inclusion criteria were divided into two groups according to the operation methods. The meshed autografts in the experimental group (6 patients) were covered with silver-containing biological dressing and those in the control group (12 patients) were covered with petrolatum gauge. The demographic data, wound condition, wound infection after skin grafting, time of first post-operative inner layer dressing change, pain score during the first inner layer dressing change, and length of hospital stay were all observed. The survival rate of skin graft, wound infection rate and total cost of hospitalization were also calculated. ResultsThe experimental group had significantly lower wound infection rate (0 vs 50.0%, P<0.05) and pain score at the first inner layer dressing change (2.50±1.05 vs 5.42±2.02, P<0.01) after skin grafting compared with the control group. The period from skin grafting to the first post-operative change of inner layer dressing was significantly longer in the experimental group compared with that in the control group (\[7.50±1.05\] d vs \[4.08±1.31\] d, P<0.01). The experimental group had a significantly shorter hospital stay compared with the control group (\[14.33±1.50\] d vs \[16.42±1.93\] d, P<0.05). Moreover, the survival rate of skin grafting in the experimental group was significantly higher than that in the control group (\[97.50±1.87\]% vs \[91.42±4.48\]%, P<0.01). ConclusionWound closing by meshed autografts with silver-containing biological dressing can increase the survival rate of skin graft, shorten wound healing time, reduce local pain, and obtain satisfactory outcome.

Abstract:ObjectiveTo explore the risk factors of in-hospital death of acute myocardial infarction (AMI) patients following percutaneous cornary intervenion (PCI). MethodsWe retrospectively analyzed the clinical data of 275 AMI patients, including the general condition, medical history, admission, intra- and post-operation condition, and complication. The patients underwent PCI in our hospital from January 2009 to September 2011. Logistic regression analysis was used to predict the risk factor of in-hospital death of AMI patients following PCI. ResultsLogistic regression analysis found that female sex (P=0.047, OR=2.91), extensive anterior myocardial infarction (P=0.044, OR=3.07), history of renal insufficiency (P=0.021, OR=7.98), cardiogenic shock (P=0.008, OR=15.71), postoperative thrombolysis in myocardial infarction (TIMI) flow grade ＜3 (P=0.016, OR=5.61), and placement of the intra-aortic balloon pump(IABP, P=0.043,OR=3.49) were associated with an increased risk of in-hospital mortality of AMI patients following PCI. ConclusionFemale sex, extensive anterior myocardial infarction, placement of the IABP, postoperative TIMI flow grade ＜3, history of renal insufficiency, and cardiogenic shock are the risk factors of in-hospital death of AMI patients following PCI.

Abstract:ObjectiveTo modify the strategy and methodology of percutaneous microwave ablation for treatment of hepatocellular carcinoma (HCC), so as to simplify the therapeutic process and improve the therapeutic effect. MethodsA total of 92 HCC patients with 149 nodes, with an average diameter of (4.9±3.4) cm, were treated by the newly designed cooling circulation microwave electrode. Continuous ablation was used for tumors with diameters less than 6 cm and ring-shaped continuous ablation was applied for tumors with diameters more than 6 cm. Microwave ablation was used to treat tumors with diameters less than 4 cm. For tumors with diameters more than 4 cm, transcatheter arterial chemoembolization (TACE) was used before microwave ablation and nutrient arteries were given priority for ablation. Ring-shaped continuous ablation strategy and double antenna ablation strategy were adopted for tumors over 6 cm. Enhanced CT scans were performed at regular intervals to evaluate the therapeutic effect and the one-, two- and three-year survival rates were observed. Results(1) The complete necrosis rates for once, twice, and multiple ablations were 76.5%(114/149), 86.6%(129/149) and 95.3%(142/149), respectively. (2) The one-, two- and three-year survival rates in our group were 94.6%(87/92), 72.9%(62/85) and 61.4% (35/57), respectively. ConclusionSingle microwave ablation is effective for small HCC. Double antenna ablation + first ablation of nutrient artery + TACE was effective for huge HCC. Ring-shaped continuous ablation strategy can greatly reduce ablation points and simplify the ablation process, which is probably a new way for in situ complete necrosis of huge HCC.

Abstract:ObjectiveTo evaluate the effectiveness of health education on AIDS among migrant construction workers, so as to search for effective ways for health education on AIDS among them. MethodsAIDS health education strategies were designed and put into practice based on baseline demand analysis. Questionnaire survey was used to evaluate the AIDS knowledge, attitude and behavior 5 months later. ResultsThe age, gender and educational background were similar among responders before and after interventions (P>0.05). The AIDS-related knowledge, awareness rates of each of the 8 AIDS questions and general awareness rate (at least 6 right answers to the 8 questions) were significantly improved after interventions (P<0.001). ConclusionProviding consulting service, handing off free education materials and other interventions are feasible and effective for AIDS prevention among migrant construction workers, and these interventions should be strentgthened to improve anti-AIDS ability among them.

Abstract:ObjectiveTo investigate the prevalence of nonalcoholic fatty liver disease (NAFLD) and its risk factors in physical examination population of Shijiazhuang area, so as to provide evidence for prevention and treatment of NAFLD. MethodsThe medical examination results (January to December in 2010) were obtained from the physical examination center of Hebei Provincial People’s Hospital and Health Service Center of Hedong Community, Shijiazhuang. A total of 20 922 subjects who lived for more than 5 years in Shijiazhuang with complete data were selected for analysis. The prevalence and risk factors of NAFLD in this population were analyzed by univariate and multivariate analysis. ResultsThe prevalence of NAFLD in the present group was 19.18%(4 013/20 922), with that in males being significantly higher than that in females (23.40%\[2 558/10 930\] vs 14.56%\[1 455/9 992\],P<0.001), and there was significant age difference. Multiple logistic regression analysis demonstrated that uric acid (UA), systolic blood pressure (SBP), body mass index (BMI), fasting blood glucose (FBG) and triglyceride (TG) were independent risk factors of NAFLD, and their relative risks increased in order. ConclusionThe physical examination population in Shijiazhuang area has a high prevalence of NAFLD. The prevalence of NAFLD is closely associated with TG, FBG, BMI, SBP and UA.

Abstract:ObjectiveTo investigate the protective effect of synthetic fibers with tourmaline on collagen-induced arthritis in rats. MethodsWistar rats were randomly divided into three groups, namely, the control (n=5), model (n=12), and protected (n=13) groups. Rheumatoid arthritis (RA) was induced by immunization with typeⅡcollagen in Freund’s incomplete adjuvant in model group and protected group. Animals in model group were protected by conventional synthetic fibers and those in protected group were protected by the synthetic fibers with tourmaline. The protective effects were evaluated by comparing the appearance, volume (water drainage method), and pathological changes of rat paws in different groups. ResultsRats in model group exhibited more severe arthrocele than those in the protected group, and there was even arthrentasis. Histological analysis showed that the model group had more severe pathological changes in both left and right hindpaws than the protected group. Since the second week, the mean volumes of left hindpaws of protected group were smaller than those of model group, with the differences at week 2, 6, 8, 10, and 12 being significantly different (P<0.05). The protected group also had a lower incidence of RA than the model group. ConclusionSynthetic fibers with tourmaline have protective effect against collagen Ⅱ-induced arthritis in rats.

Abstract:ObjectiveTo explore the feasibility of using new guided-catheterization and modified technique for hepatic artery catheterization in rabbits under direct vision. MethodsA total of 45 rabbits were evenly randomized into 3 groups. In group Ⅰ the catheterizations of hepatic artery were performed by conventional technique under direct vision; in group Ⅱ the catheterizations were performed with Seldinger technique under DSA; and in group Ⅲ the catheterizations were performed by the new guided-catheterization and modified retrograde technique under direct vision.Then the success rate, catheterization time and survival rate were compared after catheterization among the three groups. ResultsThe success rates of catheterization were 60.0%, 73.3% and 86.7% in group Ⅰ, Ⅱ, and Ⅲ, respectively, with significant difference found between group Ⅲ andⅠ(P<0.05). The periods of catheterization were (42.0±0.5) min, (31.4±0.8) min, and (25.2±0.6) min, respectively, with the periods in group Ⅲ being significantly shorter than those in groupⅡ and Ⅰ(P<0.05, P<0.01). The incidence rates of the adverse reactions and complications were 46.7%, 33.3% and 20.0%, respectively, with that in group Ⅲ being significantly less than those in groupⅡ and Ⅰ(P<0.05, P<0.01). ConclusionThe new guided-catheterization device and improved technique using transgastroduodenal artery not only can improve the quality and efficiency of hepatic artery catheterization in rabbits, but also is safe and easy-to-perform.

Abstract:ObjectiveTo investigate the safety, early and mid-term outcomes of drug-eluting stent-assisted angioplasty in treating symptomatic vertebral artery origin stenosis. MethodsFrom June 2004 to June 2008 a total of 24 patients with stroke or transient ischemic attack (TIA) due to vertebral artery (VA) origin atherosclerotic stenosis received drug-eluting stent-assisted angioplasty. Patient records were reviewed for angiographic findings, peri-procedural complications, and follow-up data. ResultsThe procedures were technically successful for all patients and the stenoses were greatly improved. All patients were available for follow-up for (19±2) months. Two patients developed mild in-stent restenosis. ConclusionOur finding confirms that drug-eluting stent-assisted angioplasty is feasible and safe for treating symptomatic vertebral artery origin stenosis, with satisfactory early outcome.

Abstract:The cholinergic anti-inflammatory pathway (CAP) is a new neurophysiologic mechanism regulating the immune system, with alpha7 nicotinic acetylcholine receptor (α7nAChR) being its potential pharmacological target. Cumulative studies have demonstrated that activation of α7nAChR can inhibit inflammation through modulating pro-inflammatory cytokines. The role of α7nAChR has been confirmed in a series of inflammatory diseases. This review is aimed to provide new insights for using α7nAChR against inflammatory diseases.

Abstract:Calcification is a common sign in breast imaging and it can be seen in many breast diseases. Appropriate classification of breast calcifications is helpful for the early diagnosis and treatment of breast cancer. This paper reviews the current diagnosis and ultrasound-guided biopsy of breast calcifications, hoping to provide new sight in the diagnosis and treatment of breast calcification.

Abstract:Measuring changes in visual function alone is not enough to assess neither the impact of eye diseases on patients nor the effectiveness of treatments. The quality of life is a parameter demonstrating all kinds of behavior capabilities, covering the human body function, psychological and mental state and social activities. It has recently become a popular parameter to evaluate the therapeutic effect for diseases. The paper introduces the current application (methods and results) of life quality study in eye diseases, hoping to provide evidence for future clinical practice.

Abstract:ObjectiveTo discuss the feasibility and safety of a novel urethral dilation procedure using antegrade insertion of guide wire through suprapubic fistula (retrograde insertion failed) to facilitate retrograde dilation for treating early stricture following urethroplasty. MethodsThirteen patients, who failed to receive retrograde insertion, developed early stricture following urethroplasty. The guide wire was inserted through a pre-made suprapubic fistula to pass the stricture with the help of flexible cystoscope. Then the guide wire was used to guide polytef catheter to perform retrograde dilation. ResultsThe procedures were successfully done in all the 13 patients. The mean operation time was (10.7±3.18) min (8-17 min), and there were no serious complications. ConclusionThe present dilation procedure is an effective and safe method for management of early stricture recurrence following urethroplasty.

Abstract:ObjectiveTo select the best plan for establishing frontline surgical team (FST) for emergency medical relief based on the characteristics of health service for non-battle military missions, hoping to provide theoretical reference and evidence for establishing FST. MethodsThis study proposed 4 options to setup FST, including establishing a new one (P1), integration of professional operation teams (P2), selected group from field medical unit (P3), and modification of existing field medical team (P4). The analytic hierarchy process (AHP) was used to analyze the relevant factors influencing the establishment of FST, in an effort to build a hierarchical model diagram and to form a judgment matrix score of four scenarios; the four programs was evaluated by consulting experts and calculating the index weights and general weights. ResultsThe consistency of matrix test results was judged as CR<0.10, with the weights of the program scores being P1 =0.213 8, P2 =0.242 0, P3=0.205 4, and P4 =0.338 8. ConclusionApplication of AHP can achieve satisfactory result in selecting optimal plan for establishing FST; the optimal option to establish FST is to convert the existing field medical team (P4).

Abstract:ObjectiveTo set up an evaluation system for hospital performance, so as to meet the requirement of health system reform, to properly allocate health resources, to effectively standardize the administration of military hospitals, and to upgrade their overall performance. MethodsAccording to the current situation, an evaluation system for performance of military hospitals, which including four domains-finance, patients, internal flow, and learning and progression, was established based on the balanced scorecard (BSC) ideology using the hospital resources planning (HRP) system, analytic hierarchy process (AHP) and Delphi method. ResultsWe established an evaluation system for the performance of military hospitals, which including four first level parameters, 13 second level parameters, and 55 third level parameters. ConclusionHRP combined with BSC for evaluating hospital performance can help the proper allocation of health resources in military hospitals, contributing to the sound, cost-saving and highly efficient administration of hospitals. Adjustment should be made in actual application, and attention should be given to the time effectiveness and scientific nature of the parameters.

Abstract:ObjectiveTo study the association of alleles and genotype frequencies of rs4402960 in insulin-like growth factor 2 mRNA binding protein 2 (IGF2BP2) gene with type 2 diabetes mellitus (T2DM) in Han population of Inner Mongolia region. MethodsAllele-specific polymerase chain reaction was used to genotype the rs4402960 polymorphism of IGF2BP2 in 360 Han population living in the Inner Mongolia region, including 166 patients with T2DM (T2DM group) and 194 normal controls (NC group). ResultsThe frequencies of T allele, TT and GT genotype of rs4402960 in T2DM group were 41.6%,12.1% and 59.0%, respectively, which were significantly higher than those in the NC group (10.6%,3.6% and 13.9%, all P<0.05). The GG genotype frequency was 28.9% in T2DM group, being significantly lower than that in the NC group (82.5%, P<0.05). Carrying T allele was a risk factor of T2DM (OR=6.02, 95%CI=4.075-8.895). ConclusionT allele of rs4402960 polymorphism in IGF2BP2 gene might be a risk factor of T2DM, and G/T polymorphism is associated with T2DM. IGF2BP2 rs4402960 might be a susceptible gene of T2DM in Han population living in Inner Mongolia region.

Abstract:ObjectiveTo evaluate the safety of ketamine combined with propofol in total intravenous anesthesia for spastic cerebral palsy children undergoing neural stem cell transplantation.MethodsSixty ASA Ⅰ-Ⅱ spastic cerebral palsy children, aged 1-12 years old, were to undergo neural stem cell transplantation. Anesthesia was induced with propofol 1.5-2 mg/kg and ketamine 1-2 mg/kg, iv, and maintained with target controlled infusion (TCI) of propofol 30-40 μg·kg-1·min-1 and ketamine 10-30 μg·kg-1·min-1. The mean arterial pressure (MAP), heart rate (HR), respiration rate (RR), and arterial oxygen saturation (SpO2) were observed during operation. The consciousness loss time, awakening time, anesthetic effect, and adverse reaction were recorded.ResultsThere were no significant differences in MAP or HR before and after infusion. The patients had decreased RR and SpO2 at 1 min and 3 min after infusion, but there is no significant difference compared with before infusion. Consciousness loss time was (50.5±9.1) s and the awakening time was (15.3±3.6) min. The effect of anesthesia was satisfactory in all the children. There was no writhing, nausea, vomiting or salivating. Three cases had respiratory inhibition and one had choke cough during operation. ConclusionKetamine combines with propofol is safe, controllable and has less side effects in total intravenous anesthesia for spastic cerebral palsy children undergoing neural stem cell transplantation.

Abstract:ObjectiveTo evaluate the effect of severe hemorrhagic shock and resuscitation on expression of EPHX2 and predictive transcription factor Sp1 in rat liver. MethodsSp1 was selected for study by software prediction of the transcription factor EPHX2. A total of 25 SD rats were randomly divided into 5 groups (n=5): control (CON) group, hemorrhagic shock (HS) group, 1 h after hemorrhagic shock and resuscitation (HSR1) group, 3 h after hemorrhagic shock and resuscitation (HSR3) group, and 6 h after hemorrhagic shock and resuscitation (HSR6) group. Real-time RT-PCR and Western blotting analysis were used to examine the expression of EPHX2 and Sp1 in the rat liver. ResultsThe expressions of EPHX2 mRNA in HS, HSR1, HSR3, and HSR6 groups were significantly lower than those in CON group (P<0.05), those in HSR1, HSR3 and HSR6 groups were significantly lower than those in HS group (P<0.05), and that in HSR1 group was significantly lower than those in the HSR3 and HSR6 groups (P<0.05). The expression of Sp1 mRNA in CON group was not significantly different from those in other groups, but the expressions in HS and HSR1 groups were significantly lower than that in the HSR6 group (P<0.05). Compared with CON group, EPHX2 protein expression was significantly lower in HSR1, HSR3, and HSR6 groups (P<0.05), and Sp1 protein expressions were similar in different groups. ConclusionEPHX2 mRNA expression is decreased in rat liver following severe hemorrhagic shock, and this decrease cannot be reversed by effective resuscitation; Sp1 is not involved in the process.

Abstract:Objective To analyze the chemical constituents of essential oil extracted from Apocynum venetum and screen the index components to evaluate essential oil in samples from different habitats. Methods Head-space-GC／MS was employed to prepare essential oil from Apocynum venetum，with HP-5 quartz capillary column，extraction temperature 90℃ ，hold 30 min，injection volume 1．5 mL .The datas were disposed by multivariate statistical analysis to screen the index components. Result 80 volatile components were analyzed and quantified. The system reproducibility was good .7 index volatile components were screened and the total content of the index components in samples from Jiangsu is higher than others. Conclusion The total contents of the index components were distinct in Apocynum venetum from different habitats. A rapid，simple and reliable method using HS-GC/MS and multivariate statistical methed was developed to analyze the volatile components which can be an alternative for quality evaluation for TCMs such as Apocynum venetum．

Abstract:【Abstract】　Objective: To investigate the inhibition effect of Brucea javanica oil self microemulsion on the p roliferation of human lung cancer cells. Methods: The human lung cancer cells A549 were cultivated with Brucea javanica oil self microemulsion . The effects of Brucea javanica oil self microemulsion on the proliferation of A549 cells were observed using MTT method and flow cytometry. Results: Brucea javanica oil self microemulsion could inhibit the growth of A549 cells in dose-dependent and time-dependentmanner . The cell cycle analysis showed that the percentage of G0 /G1 phase cells increased and S phase cells decreased. There was significant deviation (P<0.01) in interclass of the concentration of Brucea javanica oil emulsion 0.05mg/ml and 0.1mg/ml (P<0.01). The percentage of G2 / M phase cells decreased. Conclusion: Brucea javanica oil Self microemulsion could inhibit the proliferation of A549 cells in vitro and the mechanism may relate to its preventing the cell cycle in G0 /G1 phase.