DUAN Zeng-jie , CHEN Jia-yi , LIU Cheng-gang , ZHAO Zhong-hua , WANG Jiu-sheng
2014, 35(8):813-818. DOI: 10.3724/SP.J.1008.2014.00813
Abstract:Objective: To understand the status quo of the Chinese People’s Liberation Army’s (CPLA) pre-deployment trainings for peacekeeping medical contingents and propose suggestion for improvement. Methods: We conducted questionnaires, field investigations and focus group discussions among the CPLA’s participants of trainings, and a focus group discussion among foreign military officers at the CIE, SMMU, who had participated peacekeeping, to collect data in Shenyang, Beijing, Jinan, Lanzhou, and Chengdu Military Theaters of Operations(MTO). We then processed and analyzed the data with Microsoft Excel and SPSS. Results: The CPLA attaches great importance to the medical support for UN peacekeeping missions. Accordingly, it has nominated personnel of fine qualifications for pertinent trainings organized by respective MTOs. However, the conditions for training were generally poor and there was a shortage of professional teachers and appropriate textbooks. Suggestion: Establish peacekeeping training bases of the CPLA, intensify English language proficiency training, build a reserve of professional teachers, conduct scientific assessment and evaluation, and provide modularized and integrative trainings on medical support for peacekeeping, which are organized by a third-party institution at training bases.
CONG Jun , LIU Cheng-gang , ZHAO ZHong-hua , CHEN Cao , WANG Jiu-sheng
2014, 35(8):819-823. DOI: 10.3724/SP.J.1008.2014.00819
Abstract:Objective: To research and analyze organization and management on pre-deployment training of our peacekeeping medical units, and put forward suggestion for further improving. Methods: Literature reference and seminars, questionnaire. Research contains five parts: management system, training period, hardware construction and training regulation. Results: Our peacekeeping medical units on pre-deployment training organization and management system is not clear, do not have a unified training time, training condition is relatively insufficient, lack the necessary hardware facilities, standardization needs to be strengthened. suggestion: a scientific and rational organization and management system should be specialized. Build up an unified military peacekeeping medical training base.
CAI Song-liang , LIU Cheng-gang , ZHAO Zhong-hua , YANG Xing-yu , CHEN Cao , WANG Jiu-shen
2014, 35(8):824-827. DOI: 10.3724/SP.J.1008.2014.00824
Abstract:Objective: To acquaint with our peacekeeping medical units on pre-deployment training curriculum situation. Content: To research which curriculums were learned by peacekeeping medical units and how did the peacekeeper think about the significance of the curriculum. Methods: Using questionnaire, spot research and seminars to collect the data and information from 5 military area command 260 peacekeepers and 14 foreign students of COE SMMU; using Microsoft Excel software and Likert statistical method to deal with the data. Results: The most popular 9 curriculum, the most significant and the most unimportant 5 curriculum have been selected and analyzed. Suggestion: Design training modular and regulate the training content, implement differential and individualized curriculum, strengthen English training and develop the practical teaching pattern.
ZHU Jian-ling , LIU Cheng-gang , ZHAO Xiao-jun , ZHAO Zhong-hua , chencao , WANG Jiu-shen
2014, 35(8):828-831. DOI: 10.3724/SP.J.1008.2014.00828
Abstract:Objective: To research and analyze the current situation of evaluation for Peacekeeping Medical Team Pre-deployment Training and put forwards relative suggestion. Methods: the ways of reading literature、symposium、questionnaire and on-site survey, and Kirkpatrick Mode was used as a guide to analyze. Contents: according to satisfaction degree from the reflection of investigation with each layer, to analyse and discuss the assessment with the real work. Result: the assessment is not standard, the affection is not reflected brightly. Suggestion: to build up an assessment index system and make it standardization.
Fu Xiaojuan , Chen Xue-mei , Zhou Qin , Du Xiao-gang
2014, 35(8):832-836. DOI: 10.3724/SP.J.1008.2014.00832
Abstract:Objective To identify the molecular evolution of Miox and its temporal and spatial expression patterns in the evolution of Xenopus laevis embryos. Methods The temporal and spatial expression patterns were analyzed by semi-quantitative reverse-transcriptional PCR (RT-PCR) and whole-mount in situ hybridization, respectively. Results We investigated the temporal expression pattern of Miox during embryogenesis of Xenopus laevis by RT-PCR and found that hardly any expression was detected before stage 26. Miox firstly expressed at stage 28 with a very small amount. Its expression level gradually increased along with the development of the process and reaced its peak at stage 40 and kept a high stable level in later period. Compared with stage 28,34,the expression of stage 40,41,45 were upregulated significantly(P<0.05) .Compared with stage 40,the expression of stage 41 was upregulated markedly(P<0.05).But compared with stage 41,the expresssion of stage 45 was downregulated(P<0.05).The results of whole-mount in situ hybridization showed that Miox started express at stage 33 and the expression trend was consistent with RT-PCR. We also found that Miox only expressed in the pronephros tubules in the whole procss of embryo development. Conclusion Miox was tissue-specific in Xenopus laevis pronephros development, which may provide a marker for later pronephros development in the study of organogenesis.
CHEN Song , FENG Xu , ZOU Ying-xin , LUAN Jie , ZHANG Shu , ZHOU Yi , CHU Zhi-yong
2014, 35(8):837-841. DOI: 10.3724/SP.J.1008.2014.00837
Abstract:Objective To study the expression of apoptosis-associated genes and the role of apoptosis in acute radiation-induced liver injury. Methods A mouse radiation model, which was irradiated with 60Co γ ray, was established in this study, and the pathological changes were observed by light and electron microscopy for 48 hours. Western blotting analysis was used to measure the expression of some proteins (Caspase 3,Caspase 8, Bcl-2, Bcl-xL, Bax, Bad, PUMA, Slug) and TUNEL assay was employed to examine cell apoptosis in mouse liver. Results Degeneration and apoptosis were found in the liver at 4 h after irradiation and necrosis occurred at 12 h after irradiation. The peak of apoptosis with activation of Caspase 3 in liver was detected during 24-48 h after irradiation. Bcl-2 protein expression was up-regulated during 4-24 h after irradiation, then was down-regulated at 48 h; PUMA protein was up-regulated during 4-48 h after irradiation; Bcl-xL and Bad protein were up-regulated during 6-48 h after irradiation; and Bax and Slug protein were up-regulated only at 12 h after irradiation. Conclusion Up-regulation of BH3-only members (PUMA and Bad) after irradiation may be associated with the increase of apoptotic cells in the liver.
LAN Zhong-ping , GU Nan , XIONG Li-ze , SUN Yan-yuan
2014, 35(8):842-846. DOI: 10.3724/SP.J.1008.2014.00842
Abstract:Objective To observe the effect of intrathecal agmatine(AG) at the spinal cord level on morphine tolerance. Methods (1) Twenty-four SD rats were divided into four groups: control group, pure intrathecal injection(i.t.) of saline(15 μL); morphine group, i.t. morphine(15 μg/15 μL); AG group, i.t. AG (12.5 μg/15 μL); and morphine+AG group, i.t. morphine (15 μg/5 μg) and AG (12.5 μg/10 μL). Rats of the four groups were injected with 0.2 mg bee venom subcutaneously in the plantar 5 min after i.t, and the numbers of spontaneous paw withdrawal reflex were recorded within 1 hour. (2) Twenty-four SD rats were divided into three groups: control group, pure intrathecal saline(15 μL); morphine tolerance group, i.t. morphine(15 μg/5 μL) twice a day for 4 consecutive days; and morphine tolerance+AG group, i.t. morphine(15 μg/5 μL)twice a day for 4 consecutive days, on the fourth day the rats also received AG(12.5 μg/10 μL). Half of the rats were examined for thermal paw withdrawal latency and mechanical withdrawal threshold after i.t, and the other half was injected with 0.2 mg bee venom subcutaneously in the plantar 10 min after last dose; the number of spontaneous paw withdrawal reflex were recorded within 1 hour. Results (1)Compared with the control group, the numbers of flinches of intrathecal morphine and AG groups were significantly reduced(P<0.05). (2) In intrathecal morphine tolerance model, the thermal paw withdrawal latency and mechanical withdrawal threshold were significantly increased in the morphine tolerance+AG group compared with the control group (P<0.05);morphine tolerance + AG group had significantly stronger inhibitory effect against spontaneous s.c. BV-induced pain, with the number of spontaneous flinches within 1 hour decreased significantly in the morphine tolerance + AG group(P<0.05). Conclusion Intrathecal AG has synergistic effect with morphine in pain relieving, and it can also reverse the morphine tolerance induced by repeated i.t. injection.
张晨冉 , wu xiaojun , hu liuhua , he hua , jiang lei , hu guohan , ding xuehua , lu yicheng
2014, 35(8):847-851. DOI: 10.3724/SP.J.1008.2014.00847
Abstract:Objective To investigate the expression level of RIZ1 and Ki-67 in astrocytomas of different grades and its association with patient prognosis. Methods The expression of RIZ1 and Ki-67 in glioma tissues were evaluated immunohistochemically in 51 patients with gliomas. The correlation of RIZ1 immunoreactivity with clinicopathological featurees and the prognostic value of RIZ1 in patients were also analyzed. Results Immunohistochemistry showed a decrease in RIZ1 labeling index in high grade gliomas versus low grade gliomas (7.55±4.39% vs 48.83±4.34%,P<0.0001). There was a negative correlation between RIZ1 and Ki-67 immunoreactivity (Spearman r =-0.8130, P<0.0001). Clinicopathological evaluation suggested that RIZ1 expression was negatively associated with tumor grade and patient age (P<0.05). Kaplan–Meier survival analysis revealed a significantly positive correlation between levels of RIZ1 immunoreactivity and PFS (progress free survival, PFS). Univariate survival analysis revealed significant relationships between expression of RIZ1, expression of Ki-67, and prognosis. Multivariate analysis using the Cox proportional hazards model for all variables included in univariate analysis revealed that high RIZ1 expression was independent prognostic factors for patients with gliomas. These results suggest that RIZ1 expression may be a key prognostic index for glioma patient survival. Conclusion: We conclude that high RIZ1 labeling index indicates lower grade of gliomas, and associated with increased patients’ PFS and overall survival (OS). RIZ1 may be a potential tumor suppressor in the development of gliomas.
CHEN Jin , YANG Lu , CHEN Jing-bo , DING Chang-hua , LU Jian-can , HUANG Xiang-qin , HUANG Qin
2014, 35(8):852-859. DOI: 10.3724/SP.J.1008.2014.00852
Abstract:Objective To analyze the controlling status of glucose metabolic indices of outpatients with type 2 diabetes mellitus (T2DM) who were treated in our hospital for a long term, so as to provide theoretical evidence for better management of the blood glucose. Methods From March 1, to April 30, 2012, the clinical and laboratory data of the latest 3 months of 304 T2DM patients were collected. According to the hypoglycemic therapies, the patients were divided into oral hypoglycemic drug treatment group (group A), pure insulin treatment group (group B), and oral medicine combined with insulin treatment group (group C). The status of metabolism indices and the relationship between the controlling status of metabolic indices and complications were explored. Results (1) The mean hemoglobin A1c (HbA1c) of the 304 T2DM patients was (8.01±1.14)%, with only 13.16% of them reaching the recommended glycemic level (<7.0%). We also found that 74.67%(227/304) of patients had at least one complication or concomitant disease. The 2 h-postprandial glucose at diagnosis was found correlated with the number of macrovascular diseases (r=0.131,P=0.024). (2) The glycemic control in group B and C was significantly better than that in group A (all P<0.05). Sulphonylureas and biguanides were still the most commonly used hypoglycemic agents. Premix insulin analog (accounting for 50.85%, 30/59) and intensive insulin therapy (accounting for 27.12%, 16/59) were the commonly used insulin therapy. (3) Patients who performed self-monitoring of blood glucose (SMBG) a week before filling our form in group A, B, and C accounted for 27.56%, 57.63% and 50.85%, respectively. And the control of glycosylated HbA1c, fasting blood glucose and postprandial glucose of these performing SMBG was better than those not performing in the same group (P<0.01 for all). (4) Logistic regression analysis indicated that performing SMBG was an influencing factor (OR=0.379,P=0.007) of poor glycemic control (glycosylated hemoglobin A1c ≥7%). Conclusion Poor control of glycosylated HbA1c remains to be a great challenge for treatment of diabetes. Treatment strategy should be adjusted promptly when glycemic control fails to achieve the recommended level, including starting insulin therapy and adjusting amount of insulin amount.
HU Guan-zhong , JIN Zhen-dong , LIU Feng , ZHANG Min-min , WANG Dong , WANG Luo-wei , SHI Xin-gang , CHEN Jie , LI Zhao-shen , ZOU Duo-wu
2014, 35(8):860-864. DOI: 10.3724/SP.J.1008.2014.00860
Abstract:[Abstract] Objective To compare endoscopic sphincterotomy ( EST ) and endoscopic papillary balloon dilation ( EPBD ) in the treatment of extrahepatic bile duct stones in terms of similarities and differences. Methods Clinical data of 277 patients with extrahepatic bile duct stones from August 2009 to July 2011 in gastroenterology department of Shanghai Changhai Hospital were retrospectively analyzed. 168 of the 277 patients received EST and the other 109 received EPBD.The general case, stone diameter, stone extraction method, operation time, stone once complete clearance rate, postoperative hospital stay and short-term complications of EST group and EPBD group were compared. Results There was no statistic difference in the age, sex ratio, combined duodenal diverticulum between two groups .The stone diameter was (11.3+6.2 ) mm in EST group and that was (10.1+5.7 ) mm in EPBD group. There was no statistic difference between them( P=0.1056 ). There was no statistic difference in the stone extraction method between EST group and EPBD group. The operation time was (21.2+3.1)min in EST group and that was (20.7+2.5) min in EPBD group. There was no statistic difference between them(P=0.1591). The stone once complete clearance rate was 95.2% in EST group and that was 94.5% in EPBD group. There was no statistic difference between them( P=0.7828 ).The postoperative hospital stay was ( 4.1+1.9 ) day in EST group and that was ( 4.1+2.0 ) day in EPBD group. There was no statistic difference between them( P=1.0000 ).The incidence of short-term complications was 10.1% in EST group and that was 12.8% in EPBD group. There was no statistic difference between them( P=0.4822 ).The incidence of hemorrhage was 3.6% in EST group and that was 0 in EPBD group. There was statistic difference between them( P<0.05 ). The incidence of hyperamylasemia was 8.3% in EPBD group and that was 2.4% in EST group. There was statistic difference between them( P<0.05 ). Conclusion EST and EPBD each have advantages and disadvantages in the treatment of extrahepatic bile duct stones . Suitable operation mode should be chosen by the specific situation of patients and not limited to the size of the stones.
JIA Xiao-xia , WU Li-zhi , YANG Wen , SHEN Qi-jun
2014, 35(8):865-869. DOI: 10.3724/SP.J.1008.2014.00865
Abstract:Objective To apply Shapley value analysis of the game theory for evaluating the relative importance of the predictors in the linear regression when colinearity exists, and to provide a new concept of sequential importance partial R2. Methods Shapley value analysis of game theory(proposed by Shapley in 1953) was used to evaluate the influencing factors of hemoglobin(HB) in 757 normal adults, by regressing HB on four predictors including the white blood cell(WBC), red blood cell(RBC), blood platelet(PLT) and hematocrit(HCT); meanwhile, the sequential importance partial R2 was used to analyze its practical significance. Finally the estimated results of Shapley value was compared with others measures including traditional methods and recommended method. Results A succinct set of predictors including RBC, PLT and HCT was identified for establishing a multiply regression, with their relative importance values being 0.355 3, 0.012 4 and 0.553 8, respectively. The results of relative importance were consistent between Shapley value and dominance analysis. Moreover, it was found that the partial R2 of predictors had different marginal contributions in different orders. Conclusion HCT has the largest contribution to HB, followed by RBC, and PLT has the least effect to HB. The order of contributions is consistent with the correlation matrix, indicating that the relative importance of the predictors in Shapley value is reasonable.
Huang Song-qun△ , Hu Jian-qiang , Huang Xin-miao , Guo Zhi-fu , Zhou Bing-yan , Qin Ai-hong , Cao Jiang.
2014, 35(8):870-872. DOI: 10.3724/SP.J.1008.2014.00870
Abstract:Objective: To investigate the relationship between autonomic nervous reflex and early recurrence of atrial fibrillation after radiofrequency catheter ablation (RFCA), according to analyzing diving reflex test and cold pressor test. Methods: A total of 102 patients with paroxysmal atrial fibrillation were recruited. All patients underwent diving reflex test and cold pressor test before and after RFCA. The average heart rate was compared and its relationship with early recurrence of atrial fibrillation. Results: Circumferential pulmonary vein isolation was achieved in all 102 patients. Average heat rate was decreased and increased respectively by diving reflex test and cold pressor tes (P<0.05). Diving reflex decreased significantly after RFCA (P<0.05). Diving reflex was more powerful in patients with early recurrence of atrial fibrillation than without (P<0.05). Conclusion: Parasympathetic activity decreases after RFCA as evaluated by diving reflex test, which may be related with early recurrence of atrial fibrillation.
SUN Wei , XIAO Chang-yi , LI Zhi-ying , YE Hong , WANG Ya-qin , YUAN Tai-ning , LI Hong-jun
2014, 35(8):873-878. DOI: 10.3724/SP.J.1008.2014.00873
Abstract:Objective To determine whether the polypeptide antibody induced by human papillomavirus (HPV)L1 C-terminal conserved sequence polypeptide can be used to detect HPV in cervical exfoliated cell specimens. Methods Cervical exfoliated cell specimens were collected and divided into two groups: one was subjected to nested polymerase chain reaction (Nest-PCR) for detection of HPV DNA, and the other was subjected to sandwich enzyme-linked immunosorbent assay (ELISA), in which rabbit anti-polypeptide purified antibody and mice anti-polypeptide antiserum were used to determine HPV L1 in specimens. The detection rates of HPV were compared between the two groups. Part of the specimens were used for immunocytochemistry detection. Results Nest-PCR with primer MY09/11 detected 30 HPV DNA positive specimens out of a total of 296 ones, with a detection rate of 11.15%. Then the HPV DNA negative specimens were further amplified with primer GP5+/6 , and 51 (21.34%) more positive cases were identified, making the total detection rate of Nest-PCR being 30.11% (totally 81 cases). ELISA identified 91 positive cases out of 263 specimens, with a detection rate of 34.60%, which was not significantly different from that by Nest-PCR. Immunocytochemistry detection results displayed that the polypeptide antibody could specifically reveal cervical exfoliated cells infected with HPV. Conclusion The HPV L1 polypeptide antibody can satisfactorily detect the HPV infection in cervical exfoliated cells, indicating that HPV L1 polypeptide antibody has great potential in developing HPV detection kit for cervical cancer preventive screening.
Liuying , Jitianxing , Lijiangchao , Wanglijing , Yangyongxia
2014, 35(8):879-883. DOI: 10.3724/SP.J.1008.2014.00879
Abstract:[Abstract] Objective To research the serum metabonomic difference between breast fibroadenoma patients and breast invasive ductal carcinoma patients based on 1H NMR spectroscopy and to offer reliable biochemical information for clinical diagnosis. Methods The serum metabolic spectra of breast fibroadenoma patients and breast invasive ductal carcinoma patients were collected. The data were processed using Simca-p software and analyzed with principle component analysis(PCA) and orthogonal signal correction partial least squares discrimination analysis(OPLS-DA). Results There were clear separation between breast fibroadenoma patients and breast invasive ductal carcinoma patients through analysis of PCA and OPLS-DA .And the level of lipid, lactate and PC/GPC was higher in breast invasive ductal carcinoma patients than in breast fibroadenoma patients, but glutamine, taurine, creatine, glucose were comparatively reduced in various degree. The potential biomarkers were involved in many metabolic pathways including glucose metabolism, lipid metabolism and amino acid metabolism. Conclusion Distinct difference existed in serum metabolomic profiles between breast adenoma patients and breast invasive ductal carcinoma patients. These changed metabolites may serve as the potential biomarkers to help the differentiation malignant tumor from benign and to provide reference to make therapeutic schedule.
ZHANG Lin , FU Xian-guo , LIN Yu-xiang , LAN Feng-hua , WANG Zhi-hong
2014, 35(8):884-891. DOI: 10.3724/SP.J.1008.2014.00884
Abstract:Objective To construct a lentiviral vector carrying the wild-type and mutant adrenoleukodystrophy gene(ABCD1) and to investigate the effects of ABCD1 mutation on the structure and function of adrenoleukodystrophy protein(ALDP). Methods Different computational algorithms were used to predict the pathogenicity and the structural stability of ALDP mutants: H283R and P534R. Lentiviral vectors carrying wild type and mutants ABCD1 gene were constructed with pLEX-MCS, namely, pLEX-ABCD1, pLEX-ABCD1-H283R and pLEX-ABCD1-P534R. The recombinant plasmids and two packaging vectors were co-transfected into 293T cells to obtain virus, and the latter was used to infect host cells. The expression of the wild type and mutant ABCD1 mRNA in lentivirus infected cells was detected by RT-PCR. The subcellular localization and expression of the wild type and mutant ALDP were detected by immunofluorescence and Western blotting analysis. Results Bioinformatic prediction results showed that both mutations in this study were at conserved codons, suggesting a pathogenic nature. Overexpression of the wild type and mutant ABCD1 mRNA was detected by RT-PCR in lentivirus infected cells. Immunofluorescence study and Western blotting analysis showed overexpression of the wild type ALDP and lower expression of the mutant ALDP, with no subcellular mislocalization of the mutant ALDP detected. Conclusion We have successfully constructed a recombinant lentiviral vector carrying the ABCD1 gene and assessed the effects of the ABCD1 mutations on the expression and localization of ALDP, providing evidence for understanding the pathogenic mechanism of ALD.
LÜ Quan-zhen , YAN Lan , JIANG Yuan-ying
2014, 35(8):892-897. DOI: 10.3724/SP.J.1008.2014.00892
Abstract:Stable isotope labeling by amino acids in cell culture (SILAC) is an in vivo metabolic labeling technique based on the abundance ratio of mass spectrometry. Combined with different methods of proteins affinity enrichment, SILAC can obtain sensitive, accurate and quantitative information of proteins. This article reviewed the history, rationale, characteristics and application of SILAC, with special focus on its role in studying protein interactions with small molecules, quantitative proteomics and post-translational modification of proteins.
赵静 , zhengjianming , yuyongwei
2014, 35(8):898-900. DOI: 10.3724/SP.J.1008.2014.00898
Abstract:The fusion gene between echinoderm microtubule-associated protein-like 4 (EML4) and anaplastic lymphoma kinase(ALK) has recently been identified as a new molecular target of non-small cell lung cancers(NSCLC).The fusion gene has higher morbidity in NSCLC patients who are young , never or light smoking history and adenocarcinomas.This review emphasizes the biological,detection method,clinical characteristics and the therapeutic application of EML4-ALK in NSCLC.
2014, 35(8):901-906. DOI: 10.3724/SP.J.1008.2014.00901
Abstract:Obesity is a complex metabolic disorder, which involves central mechanisms that control food intake and energy expenditure.The basic mechanisms that lead to obesity are not fully understood, however, oxytocin undoubtedly plays a role in regulating body weight. Previous studies have shown that central or peripheral oxytocin administration induces anorexia. But recently, some researchs have shown that rodents lack of oxytocin or its receptor develop late-onset obesity without changing their total food intake, which indicates the importance of oxytocin to energy metabolism. The effects of oxytocin on energy balance regulation is discussed in detail, especially the role of oxytocin on appetite regulation, thermoregulation, and energy metabolism.
LI Hu , CHENG Ming-kun , WANG Hui , CHEN Yan , SU Xian-hao , WANG Jin , HU Zhi-de , QIN Qin , LIU Yun , DENG An-mei
2014, 35(8):907-909. DOI: 10.3724/SP.J.1008.2014.00907
Abstract:Objective To explore the discrimination efficiency of SDS-NaOH solution for gram-positive and gram-negative bacteria in urine. Methods Seventy nine urine samples were treated with SDS-NaOH solution for 5 minute. The bacteria accounts before and after treatment were determined by UF-1000i urine analyzer and bacteria decreased rate (BDR) was calculated. Receiver operation characteristics (ROC) curve analysis was used to assess the discrimination efficiency of BDR. Results BDR was decreased in gram-negative bacteria contained urine (P<0.01). The area under curve (AUC) of BDR for discriminating gram-positive and gram-negative bacteria was 0.70 (95% confidence interval (CI): 0.57-0.82). At cuf-off value of 0.05, the discrimination sensitivity and specificity of BDR for gram-positive and gram-negative bacteria were 0.83 (95% CI: 0.63-0.95) and 0.46 (95% CI: 0.32-0.59), respectively. Conclusion SDS-NaOH solution is useful to discriminate gram-positive and gram-negative bacteria in urine.
cuihaiming , zhangjiayou , liaodening
2014, 35(8):910-914. DOI: 10.3724/SP.J.1008.2014.00910
Abstract:[Abstract[已经根据中文重新翻译]] Objective To explore the feasibility and effectiveness of catheter ablation of Idiopathic Right Ventricular Outflow Tract Premature Ventricular Contractions (RVOT-PVCs) guided by CARTO3 electroanatomical navigation system. Methods 49 patients with RVOT-PVCs underwent catheter ablation were divided into two groups, 3D group using CARTO3 guide ablation (n = 29), the conventional group using X-Ray guide ablation (n = 20), compared two groups of operation time, X-ray fluoroscopy time, X-ray fluoroscopy exposure, cost,complication, achievement and recurrence rate. Results The immediate success rate in 3D group was 100% (29/29), in the conventional group was 95% (19/20); There were 3 cases of complications in the conventional group, but no significant complications in 3D group. Fluoroscopy time of CARTO 3 group was less than X-Ray group[ (1.3±3.6) min vs (44.2±28.2)min,P<0.01], with a correspondingly low radiation exposure[(93±242) mGy vs (3 919±2 309) mGy, P<0.01]. There was no statistically significant difference in total operation time of two groups.In 20/29 (69.0%) RVOT cases, electroanatomical mapping avoided fluoroscopy entirely. Conclusion Our study shows that using 3D electroanatomical navigation system (CARTO 3 system) in RVOT-PVCs ablation is effective and safe,it can markedly reduce fluoroscopy exposure.In many cases, non-fluoroscopic RFCA is feasible.
XING Rong-chun , ZHENG Jun , CHEN Ping , ZHENG Wei-hong , LIU Wei , HE Zheng , YAO Ru-cheng
2014, 35(8):915-919. DOI: 10.3724/SP.J.1008.2014.00915
Abstract:Objective To investigate the role of E-cadherin in resistance of hepatocellular carcinoma to treatment with epidermal growth factor receptor tyrosine kinase inhibitor (EGFR-TKI). Methods Four liver cancer cell lines were used in the present study, namely, HepG2, BEL-7404, SK-HEP-1 and MHCC97. E-cadherin protein expression in the four cell lines was examined by Western blotting analysis. MTT method was used to examine the relationship between E-cadherin expression and inhibition rates of liver cancer cells treated with EGFR-TKI. Results E-cadherin was positive in HepG2 and BEL-7404 cells, and they were sensitive to EGFR-TKI treatment; the survival rates of HepG2 and BEL-7404 cells were correlated with the concentrations of PD153035 and gefitinib (P<0.05). E-cadherin was negative in SK-HEP-1 and MHCC97 cells, and they were both resistant to EGFR-TKI treatment, and there was no association between SK-HEP-1 and MHCC97 cell survival rates and concentrations of PD153035 and gefitinib(P>0.05). The sensitivity of SK-HEP-1 cells to EGFR-TKI was significantly increased after transfected with E-cadherin compared with those transfected with empty vectors(P<0.05). Conclusion E-cadherin plays an important role in regulating the sensitivity of EGFR molecule targeted therapy.
qiushichao , renjiangong , 罗晖 , huxuejian , houyuanhua
2014, 35(8):920-923. DOI: 10.3724/SP.J.1008.2014.00920
Abstract:【】Objective To investigate the relationship between brain-derived neurotrophic factor (BDNF) and vascular endothelial growth factor(VEGF)with the carotid atherosclerosis in type 2 diabetes mellitus(T2DM). Methods A total of 120 T2DM patients were assigned to non carotid atherosclerosis group( group B, n=56) and the carotid atherosclerosis group( group C,n=64) bycarotid artery ultrasound detection. 60 healthy controls were recruited from health check-up populations(group A).Clinical data was collected,SandSthe level of BDNF,SVEGF were determined by ELISAS.Results The level of BDNF (9.97±2.59),(6.88±3.10),(5.62±3.41)ng/ml of group A, group B, group C was gradually decreased (P<0.05); The level of VEGF (49.29±20.23), (61.29±22.05),(71.34±20.30)pg/ml of the there groups was gradually increased (P<0.05).Conclusions The level of BDNF is significantly decreased and the VEGF is significantly increased in T2DM with carotid atherosclerosis, suggesting that low level of BDNF and high level of VEGF are correlated with the occurrence and development of carotid atherosclerosis.
Tai ZongGuang , Zhu QuanGang , Zhang Wei , Wang Xiaoyu , Dai ZiYuan , Zhang Lijuan , Gao Shen
2014, 35(8):924-927. DOI: 10.3724/SP.J.1008.2014.00924
Abstract:Objective A HPLC method for quantitative determination of clopidogrel hydrogen sulfate and its related substances was established .We also compared the content of effective components and impurity of clopidogrel tablets from three manufactures. Method an UL-TRON ES-OVM column (5μ m, 4.6mm × 150 mm) was used, The mobile phase consisted of a mixture of acetonitrile and 0.01M potassium dihydrogen phosphate solution (20:80). The flow-rate was 1ml/min, the column temperature 17℃, the UV detection was performed at 220 nm. Result Clopidogrel Hydrogen Sulfate and its related substances in 50 mg/L~117 mg/L and 1.52mg/L~30.04 mg/L were respectively in good linear relationship (correlation coefficient r = 0.9998). Conclusion The method is accurate and has good specificity. It can be used for the determination of Clopidogrel Hydrogen Sulfate and its related substances.
2014, 35(8):928-930. DOI: 10.3724/SP.J.1008.2014.00928
Abstract: