Abstract:Objective To observe the effects of miR-429 on inflammatory infiltration, differentiation and proliferation of hepatocelluar carcinoma (HCC). Methods HCCLM3 cells were sorted into positive and -negative cells using magnetic activated cell sorting (MACS) according to EpCAM marker. The EpCAM-positive and negative cells were transfected with AntagomiR-429 and miR-429mimic to construct over-expressed and down-regulated cells of miR-429, respectively. To validate the effect of transfection, control cells and constructed cells were tested by flow cytometry (FC) and real-time polymerase chain reaction (PCR). The proliferative curves of transfected cells were plotted using cell counting kit-8 (CCK8) assay. Furthermore, tumor-bearing mice model was established by subcutaneous inoculation with transfected cells and related control cells into non-obese diabetic, severe combined immunodeficient mice (NOD-SCID). Mice were sacrificed after 6 weeks, and protein expressions of Ki67, AFP and F4/80 in tumors were determined via immunohistochemistry (IHC) to examine the status of proliferation, differentiation and inflammatory infiltration, respectively. Results The proportion of EpCAM-positive cells was decreased by down-regulating miR-429, and EpCAM-negative cells could partially transfer to EpCAM-positive cells by miR-429 over-expression. In vivo study showed that over-expressing miR-429 in EpCAM-negative cells cound enhance the tumor forming ability; whereas EpCAM-positive cells had an inhibited tumor-formation when down-regulating miR-429. Interestingly, F4/80, a marker of macrophage, was strongly stained in tumor tissue with miR-429 over-expression. It was also found that in tumor tissue, Ki67 and AFP staining was suppressed with miR-429 down-regulation. Meanwhile, compared to the control, miR-429 significantly accelerated the proliferation of HCCLM3 in vitro compared with the control (P<0.05). Conclusion miR-429 can regulate the proportion of EpCAM-positive cells of HCC in vitro and in vivo, and greatly increase the inflammatory infiltration and proliferative ability, accompanied by decreased differentiation.

Abstract:Objective To investigate the effect of activating α7 nicotinic acetylcholine receptor (α7nAchR) on cerebral cortical neurons injury induced by oxygen-glucose deprivation (OGD) and the possible mechanism. Methods Cerebral cortical neurons cultured for 7 d were randomly divided into three groups: control group, OGD group (Cells experienced a 12 h oxygen-glucose deprivation) and OGD group treated with PNU-282987 (Cells experienced a 12 h oxygen-glucose deprivation with PNU-282987 pretreatment for 24 h). Cell viability was determined by CCK-8 assay, lactate dehydrogenase (LDH) was examined to reflect cell injury, apoptosis and reactive oxygen species (ROS) production were analyzed by flow cytometry, and expression of hemeoxygenase-1 (HO-1) and hypoxia inducible factor-1α (HIF-1α) were detected by Western blotting analysis. Results OGD resulted in cell death, LDH increase, and cell apoptosis. Compared with the OGD group, PNU-282987 pretreated group had significantly increased cell survival (P<0.05), significantly decreased LDH level and ROS production (P<0.05), and significantly inhibited cell apoptosis (P<0.05). Meanwhile, HO-1 protein expression was significantly increased and HIF-1α protein expression was significantly reduced in PNU-282987 pretreated group compared with the OGD group (P<0.05). Conclusion Activation of α7nAchR can protect cerebral cortical neurons against OGD-induced injury, which may be related to the anti-oxidative stress.

Abstract:Objective To explore whether exercise training can ameliorate estrogen deficiency-induced depression-like behavior and its mechanisms, so as to provide evidence for treating menopausal depression with exercise. Methods A total of 60 female adult C57BL/6J mice were randomly assigned to Sham-sedentary (Sham), Sham-exercise (Sham+Ex), Ovx-sedentary (Ovx) and Ovx-exercise (Ovx Ex) groups. Sham operation or bilateral Ovx was performed. After recovery for 1 week, Sham Ex and Ovx Ex mice were adapted to the treadmill for 5 weeks. We use sucrose consumption test, open field test and tail suspension test to inspect the mice of behavior change; then the mice were decapitated, and the serum and hippocampus samples were collected. 5-hydroxytryptamine (5-HT), indoleamine 2,3-dioxygenase (IDO) and inflammatory cytokine in the hippocampus were determined using a commercial ELISA kit. Results Compared with Sham mice, Ovx mice developed depression-like behaviors, including loss of interests, decreased exploratory behavior and increased behavioral despair, and exercise training could ameliorate these depression-like behaviors. Compared with the Sham group, Ovx group had significantly decreased hippocampal 5-HT level (P<0.01) and significantly increased IDO level (P<0.05) in the hippocampus, which were reversed by exercise training. The hippocampal levels of proinflammatory cytokines, IL-1β (P<0.05), IL-6 (P<0.01) and IFN-γ (P<0.01) in Ovx group were significantly increased compared to Sham mice, while the anti-inflammatory cytokines, IL-4 (P<0.01) and IL-10 (P<0.05), were significantly decreased in Ovx mice; exercise training could also reverse the alterations of IL-1β, IFN-γ and IL-4 levels in Ovx mice. Conclusion Exercise training can ameliorate estrogen deficiency-induced depression-like behaviors, which may be through improving inflammation in the hippocampus.

Abstract:Objective To explore the lipopolysaccharide (LPS)-induced changes of heat shock protein A12B (HSPA12B) expression and its effect on the permeability of human umbilical vein endothelial cells (HUVECs). Methods The HUVECs cultured in vitro were divided into four groups: control group (without any treatment); LPS group (1 μg/mL LPS); LPS +pIRES2-EGFP-HSPA12B-3Flag group(The HSPA12B gene overexpression plasmid was transiently transfected into HUVECs and then LPS of 1 μg/mL was added); and LPS+pIRES2-EGFP-3Flag group (The negative control plasmid was transiently transfected into HUVECs and then LPS of 1 μg/mL was added). The transendothelial electrical resistance (TEER) of HUVECs was measured by MERSST×01 Electrode. The expression of VE-cadherin was studied by flow cytometry. RT-PCR and Western blotting analysis were used to examine the expression changes of HSPA12B mRNA and protein in HUVECs stimulated with LPS at various time points. Results It was showed that after stimulated with LPS, HSPA12B mRNA and protein were gradually upregulated, and peaked at 12 h. HSPA12B significantly increased the TEER value of HUVECs (P<0.001), and it could completely offset the decline of TEER value induced by LPS(P<0.001). Besides, HSPA12B could cause upregulation of VE-cadherin expression. Conclusion HSPA12B can reduce the permeability of vascular endothelial cells by up-regulating the expression of VE-cadherin, thus protecting the vascular endothelial barrier function of endothelial cells.

Abstract:Objective To study the inhibitory effect of bone marrow mesenchymal stem cells (BM-MSCs) on the proliferation and invasion of human renal cell carcinoma cell line A498. Methods Human renal cell carcinoma cell line A498 cells were co-cultured with BM-MSCs or human dermal fibroblasts-adult (HDF-a) cells separately, and then cell proliferation was detected by flow cytometry and cell invasion ability was detected by transwell assay in each group. qRT-PCR was used to exam Snail and E-cadherin mRNA expression in A498 cells, HDF-a-treated A498 cells and BM-MSCs-treated A498 cells. Results BM-MSCs greatly inhibited the proliferation and invasion of A498 cells (P<0.05). qRT-PCR showed that co-culture with BM-MSCs significantly down-regulated Snail mRNA expression (P<0.05) and up-regulated E-cadherin mRNA expression (P<0.05) in A498 cells. Compared with BM-MSCs, HDF-a played an opposite role as for the above items. Conclusion BM-MSCs play an important role in the proliferation and invasion of LNCaP cells, which may cast new light for the biotherapy of human renal cell carcinoma cancer.

Abstract:Objective To explore the effect of anatomic liver resection (AR) and non-anatomic liver resection (NAR) on the prognosis of patients with hepatocellular carcinoma (HCC). Methods The clinical data of 721 HCC patients, who were treated between July 2008 to July 2009, were collected from Eastern Hepatobiliary Surgery Hospital; the patients included 317 undergoing AR and 404 undergoing NAR. The prognosis of the patients was compared between the two groups by using the Kaplan-Meier method and log-rank test. The influence factors of the prognosis were analyzed by using the Cox proportional hazards regression model. The propensity score matching method was used to eliminate the selection bias in clinical and pathological features. Results The 1-year, 3-year and 5-year overall survival(OS) rates of the 721 patients were 85.9%, 64.7% and 51.5%, respectively; and the 1-year, 3-year and 5-year disease free survival (DFS) rates were 59.3%, 34.0% and 25.5%, respectively. AR conferred better OS than NAR (93.1%, 74.5% and 62.5% vs 80.2%, 56.8% and 42.9%, P<0.001, respectively); the same was also true for DFS rates (69.3%, 41.3% and 34.9% vs 51.4%, 38.3% and 18.7%, P<0.001, respectively). After propensity matching, AR still achieved better prognosis than NAR (OS rates: 93.9%, 73.3%, and 59.4% vs 86.0%, 62.8%, 52.8%,P=0.010; DFS rates: 67.9%, 37.5% and 31.3% vs 56.8%, 33.1% and 22.6%,P=0.024). The results of multivariate analysis showed that the tumor size, tumor number, capsule, liver cirrhosis, microvascular invasion, and method of liver resection were the independent risk factors of OS; and blood transfusion, tumor size, tumor number, capsule, liver cirrhosis, microvascular invasion, and method of liver resection were the independent risk factors of DFS. However, the OS and DFS rates were not significantly different between AR and NAR groups in patients with hepatocirrhosis (P=0.767 and P=0.638, respectively). AR group achieved better prognosis than NAR group in the HCC patients without cirrhosis (P<0.001). Conclusion AR can achieve better prognosis than NAR in HCC patients, but for patients with hepatocirrhosis NAR should be recommended in order to retain better liver function.

Abstract:Objective To investigate the effect of silk fibroin (SF) on degradation and biocompatibility of poly (L-lactic acid-co-ε-caprolactone)(P[LLA-CL]) in vivo. Methods The scaffolds of P(LLA-CL) (w/w=1:1) blended with 25% of SF (SF/P[LLA-CL]) and P(LLA-CL) were prepared by electrospinning. Both kinds of scaffolds were subcutaneously implanted in 45 6-month-old rats for up to 6 months to evaluate their degradation and biocompatibility characteristics. Results Pathological sections showed P(LLA-CL) scaffold become swollen and began to separate into different layers after 3 months, and then become broken after 6 months; while SF/P(LLA-CL) scaffold largely maintained its structure after 6 months. Immunohistochemical staining showed a large number of macrophages on the surface and in P(LLA-CL) scaffolds 1 month after implantation, and they could still be found 3 months after implantation, accompanied by foreign body giant cells; while no obvious macrophages or foreign body giant cells were found in SF/P(LLA-CL) scaffolds at different time points. Examination of inflammatory gene expression showed that TNF-α and IL-10 expression in P(LLA-CL) scaffolds was significantly higher than that in SF/P(LLA-CL) scaffolds 1 week after implantation (P<0.05), the same was also true for TNF-α, IL-1β and IL-10 expression 1 month after implantation (P<0.05), for TNF-α and IL-10 expression 2 months after implantation (P<0.05), for TGF-β expression 3 months after implantation (P<0.05), and for IL-1β and TGF-β expression 6 months after implantation (P<0.05). Conclusion SF incorporation can delay degradation, reduce inflammation, and improve the biocompatibility of P(LLA-CL) scaffolds, which may provide reference for scaffold design in tissue engineering.

Abstract:Objective To study the interaction of γ-cyclodextrin (γ-CD) and its two derivatives with rocuronium and vecuronium. Methods The interactions of γ-CD and its two derivatives HS-7, Sugammadex with rocuronium and vecuronium were characterized by Isothermal Titration Calorimetry (ITC), and the thermodynamic constant and the binding constant was determined. The binding constant and inclusion ratio of γ-CD with rocuronium and vecuronium was observed by ultraviolet-visible spectrophotometry; the binding ability of three cyclodextrins with rocuronium and vecuronium was analyzed by nuclear magnetic resonance spectroscopy COSY and ¹HNMR. Results The results of ITC showed that HS-7, Sugammadex combined with rocuronium and vecuronium in a 1:1 molar ratio, and γ-CDs combined with rocuronium in a 2:1 molar ratio, with the binding constant of HS-7 and rocuronium being (3.44±2.18)×10⁷ L/mol, HS-7 and vecuronium being (5.80±1.83)× 10⁶ L/mol, Sugammadex and rocuronium being (1.04±0.34)× 10⁷ L/mol, Sugammadex and vecuronium being (2.53±1.07)×10⁶ L/mol, and γ-CD and rocuronium being (2.84±3.41)×10⁴L/mol. The results of ultraviolet-visible spectrophotometry revealed that γ-CDs combined with rocuronium and vecuronium in a 2:1 molar ratio, with the binding constant of γ-CD and rocuronium being 6.93×10⁴L/mol, and of γ-CD and vecuronium being 5.17×10⁴ L/mol. The results of nuclear magnetic resonance spectroscopy suggested that the binding ability of HS-7 with rocuronium and vecuronium was stronger than that of Sugammadex and γ-CD. Conclusion The binding ability of HS-7 with rocuronium and vecuronium is stronger than that of Sugammadex and γ-CD, and the three methods used in this study (ITC, ultraviolet-visible spectrophotometry and nuclear magnetic resonance spectroscopy) can characterize the inclusion complex from different perspectives.

Abstract:Objective To use the inclusion technology for improving the solubility and dissolution rate of baicalein from monolithic osmotic pump tablet containing inclusion complex of baicalein by observing the effects of the core and coating on in vitro drug release. Methods Baicalein-inclusion complex was prepared by the inclusion technique, and its solubility and dissolution rate were determined. The percent of cumulative release was used to evaluate the drug release profile in vitro. Single factor analysis was used to study the effects of NaCl and PEO amounts, coating weight and plasticizer amount on drug release. Then orthogonal design was used to select the optimal formulation of monolithic osmotic pump tablet containing baicalein-inclusion complex. Results The solubility and dissolution rate of baicalein were greatly enhanced when prepared into inclusion complex. Orthogonal design results indicated that PEO content in the tablet core and plasticizer PEG 400 in the coating had significant effects on the drug release, and the optimum formulation was: baicalein-inclusion complex 180 mg, NaCl 100 mg, PEO 80 mg, coating weight 4% and plasticizer 9%. The tablets with optimized formula achieved the desired zero-order release profile (r=0.997 8) within 12 hours and the cumulative release was higher than 88%. Conclusion Monolithic osmotic pump tablet of baicalein has been successfully prepared using inclusion complex as the intermediate, and the release behavior accords with zero-order kinetics equation.

Abstract:Objective To investigate the association of circulating HER-2 level with metabolic syndrome in middle-aged and elderly population. Methods We conducted a population-based cross-sectional study with 687 participants aged 40 years or older from a community in Chongming District, Shanghai, China. Serum HER-2 levels were measured using ELISA. Metabolic syndrome was defined according to the updated National Cholesterol Education Program Adult Treatment Panel Ⅲ (NCEP ATP Ⅲ) criteria for Asian-Americans. Results After adjusting for other influencing factors, we found that the participants with metabolic syndrome (n=370) had significantly higher HER-2 level compared with the control group (n=317) ([12.4±2.3] ng/mL vs [8.2±1.6] ng/mL, P<0.001). The serum HER-2 level increased (P<0.001) with the accumulation of the metabolic syndrome components. After adjusting for potential risk factors, multiple logistic regression analysis showed that the highest quartile of serum HER-2 was associated with increased risk of metabolic syndrome, with a fully adjusted odds ratio (OR) of 1.35 (95% confidence interval [CI]: 1.14-2.31), P<0.001). Conclusion Serum HER-2 is independently associated with metabolic syndrome in middle-aged and elderly Chinese in this study.

Abstract:Objective To explore the value of prostate biopsy guided by transrectal ultrasound combined with MRI compartmental localization in the diagnosis of prostate cancer. Methods Fifty patients with elevated prostate specific antigen levels were recruited in this study. All the patients underwent MRI scan before biopsy and the prostate was divided into 12 compartments accordingly. The direction and region of the division were consistent with the ultrasound. Systematic 12-samples needle biopsy was performed and additional biopsy was done for the suspected compartments. According to histopathology, the positive detection rates of systematic 12-samples needle biopsy and systematic biopsy combined with added punctures of suspected compartments were analyzed and compared. Results Prostate cancer detection rate of systematic biopsy combined with punctures of suspected compartment was significantly higher than that of systematic 12-samples needle biopsy (42.0% vs 36.0%, P<0.05). Systematic biopsy combined with punctures of suspected compartment caused hematuria in 5 cases, bloody stools in 13 cases, and fever in one, and there were no other complications. Conclusion Transrectal ultrasound combined with MRI compartmental localization can improve the outcome of prostate biopsy, benefiting the early diagnosis of prostate cancer.

Abstract:Zinc finger protein ZBTB20 is a novel member of the ZBTB zinc finger protein family and shares closest homology with BCL-6 and PLZF. Recent studies on genetically modified mice and patient cohort have revealed that ZBTB20 plays indispensable roles in multiple organs and systems, including the nervous system, liver, pancreatic islets, and immune system. ZBTB20 is an essential regulatory factor in a variety of physiological processes, such as ontogenesis, pain sensation, learning and memory, as well as glucose and lipid metabolism. Meanwhile, recent researches have also showed a close relationship between the abnormality or dysfunction of ZBTB20 and pathophysiological procedures of diseases, such as maldevelopment, mental retardation, tumors and metabolic disorders. As a key repressor governing AFP gene transcription, ZBTB20 exerts a great influence on the gene inactivation of liver AFP after birth and is closely related to the prognosis of hepatocellular carcinogenesis. In humans, the deletion of ZBTB20 is detected in diverse tumors and its point mutation leads to Primrose syndrome. This review summarizes the current knowledge concerning the biological functions of ZBTB20.

Abstract:Proteoglycan-induced arthritis (PGIA) is a unique systemic autoimmune disease model of spondyloarthritis (SpA) induced by intraperitoneal immunization of either BALB/c or certain C3H colonies with cartilage proteoglycan. This model is currently the only systemic autoimmune mice model with axial skeleton manifestation, which has become a focus of study in SpA modeling. Here in this review we summarize the methods of animal model building, phenotype identification, immunological characteristics and pathways involved in bone remodeling of PGIA based on previous studies, hoping to provide references for study on SpA animal model.

Abstract:Nano-magnetic contrast agent has become one of the important research directions of MRI contrast agent; its unique physical and chemical properties and biological characteristics present a promising application prospect and play an increasingly important role in clinical and research work. Nano-magnetic contrast agent with special physicochemical and biological properties could be swallowed by human macrophages easily, causing changes of MRI signal intensity in lesion and possessing application prospect for quantitative diagnosis. This review discusses the recent progress on nano-magnetic contrast agent and their applications for imaging macrophages in human diseases.

Abstract:A quarter of the psoriasis patients develop psoriatic arthritis, and it would be beneficial if a preparation which can be used to treat the skin and joint symptoms at the same time. Currently these biologic agents could serve both include tumor necrosis factor inhibitors such as adalimumab, etanercept, infliximab, golimumab and certolizumab; anti-IL-12/IL-23 p40 antibodies such as ustekinumab; and anti-IL-17 agents such as secukinumab, brodalumab. Recently another group of small molecule agents has been found for the purpose: phosphodiesterase-4 inhibitor, apremilast, JAK inhibitor, tofacitinib and INCB018424. This review provides an overview on the agents that can be used for the concurrent treatment of skin and joint psoriasis.

Abstract:Objective To explore a fast, effective and noninvasive method of genomic DNA extraction. Methods We compared the genomic DNAs extracted by two DNA extraction methods of potassium iodide (KI) and Kit from saliva (fresh and placed a week at room temperature), and PCR amplifications were done for TP53 and PRB-3 genes from their extracted DNA. Ninety-nine healthy children and adults were recruited and their saliva samples were collected to detect the stability of DNA extracted by KI. Results High quality genomic DNAs were extracted by both methods from the fresh saliva. The DNA yield based on KI was (1.91±0.15) μg, with the D₂₆₀/D₂₈₀ being 1.99±0.05, and that of Kit was (2.64±0.34) μg, with the D₂₆₀/D₂₈₀ being 1.81±0.02. Although the extracted DNAs had obvious degradation from saliva samples placed for a week at room temperature, the expectant DNA fragments were successfully amplified by PCR for TP53 and PRB-3 genes using these extracted DNAs. Although the genomic DNAs from saliva samples of 99 healthy volunteers by the method of KI showed individual difference (DNA yield was [1.89±0.46] μg), the DNA quality was stable and reliable (D₂₆₀/D₂₈₀ was 1.96±0.10). Conclusion KI method for extracting genomic DNA from saliva is not only cheap and highly effective, but also noninvasive, making it suitable for large-scale epidemiological studies.

Abstract:Objective To compare the sensitivity and specificity of the new CpG island methylation phenotype (CIMP) selection marker with the classical CIMP selection marker, and to analyze the clinical pathological features of CIMP lung cancer. Methods Genomic DNA was extracted from 50 cases of lung cancer tissues and the corresponding adjacent normal lung tissues in Changhai Hospital. Methylation statues of new CIMP selction marker (SHISA3, CTSL1, C1ORF103 and TMEM200B) and the classical CIMP selection markers (CACNA1G, IGF2, NEUROG1 and RUNX3) were determined by methylation specific PCR, and the results were analyzed by SPSS software. Results Notable methylation was found in the lung caner tissues, with the methylation levels of the eight studied genes in the lung cancer tissues being significantly higher than those in the adjacent tissues (P=0.014). RUNX3 gene methylation was significantly related to lymph node metastasis and PS score (P=0.017, P=0.018). The methylation rate in lung cancer patients aged over 60 was significantly higher than those aged younger than 60 years old (P=0.031). Smoking showed significant influence on CTSL1 gene methylation (P=0.018). Conclusion CpG island methylation phenotypic lung cancer has unique clinical pathological features. Both the new and classic CIMP markers are of high sensitivity and specificity for the diagnosis of lung cancer.

Abstract:Objective To observe the relationship of pre-operative serum brain natriuretic peptide (BNP) with risk of acute kidney injury (AKI) after cardiac surgery. Methods We collected 182 patients undergoing cardiac surgery at Department of Cardiac Surgery, Changhai Hospital from Jan. 2012 to Jan. 2013. The surgical procedures included coronary artery bypass graft (CABG),valve replacement, and combined surgery (CABG+valve replacement). According to the preoperative BNP levels, the patients were divided into five groups in an ascending order. The definition and classification weres accorded to the latest AKI KDIGO (Kidney Disease: Improving Global Outcomes) guideline; the AKI incidence was obtained from patients with different BNP levels. Receiver operating characteristic (ROC) curves were used to evaluate the value of BNP level as a predictor of AKI. Results The average age of the 182 patients was (68.6±8.9) years old, with male being 65.9% (120/182). The surgery procedures including 82 CABG (45.0%),57 valve replacement (31.3%), and 43 combined surgery (CABG valve replacement) (23.6%). We found that age, basic renal function and pre-operative BNP levels were significantly different between the five BNP groups (P<0.05). The incidence of AKI was 33.5% (61/182) in this study. The incidence rates of stage 1 AKI and total AKI significantly increased with increase of BNP levels in the five BNP groups (P<0.05). As a predictor of AKI ROC curves, BNP area under the curve (AUC) was 0.687 (95% CI: 0.607-0.768). Conclusion Pre-operative BNP level is associated with post-operative AKI in high risk patients undergoing cardiac surgery, and it is a potential predictor of AKI risk stratification after cardiac surgery.

Abstract:Objective To evaluate the clinical value of Camdessanché's criteria for diagnosis of patients with sensory neuronopathies (SNN). Methods We retrospectively analyzed the clinical, biochemical and electrophysiological data of 6 patients diagnosed as having SNN and 6 patients with non-SNN disease (but with similar symptoms), who were treated in Changhai Hospital from Dec. 2008 to Nov. 2014. Then the Camdessanché's criteria were used for diagnosis of SNN in the 12 patients, and the results were compared with those of empirical diagnosis. Results Four of the 6 SNN patients were classified as probable to have SNN by the Camdessanché's criteria; and two of the 6 SNN patients and one of the 6 non-SNN patients were classified as possible to have SNN. Conclusion The “probable” diagnosis of SNN in the Camdessanché's criteria has a greater value for diagnosis of SNN in Chinese, which still needs to be further confirmed by large sample study.

Abstract:Objective To analyze the factors influencing brachial-ankle pulse wave velocity (baPWV) and to establish a logit model for predicting baPWV. Methods The data of 4 159 cases who underwent health examination from 2010 to 2014 in our hospital were retrospectively analyzed. The parameters included gender, age, systolic blood pressure, diastolic blood pressure, pulse, fasting glucose, triglyceride, cholesterol, alanine aminotransferase, glutamyl transpeptidase and cholesterol, low density lipoprotein (LDL), high density lipoprotein (HDL), and uric acid. Logistic regression was used to explore the influencing factors of baPWV, and a regression model was established to predict baPWV and it was evaluated. Results Univariate analysis showed that, except for HDL, all the other parameters above were significantly different between normal baPWV group and abnormal baPWV group (P<0.05). Multivariable analysis yielded the following logit model: logit (p)=-17.888+0.001×uric acid 0.004×alanine aminotransferase 0.105×fasting glucose 0.023×pulse 0.032×diastolic blood pressure 0.061×systolic blood pressure 0.092×age 0.411×sex, which showed a correct predicting rate of 79.6% for baPWV in health examination population, with the ROC area being 0.869 (95%CI:0.859-0.879). Conclusion The baPWV values can be influenced by gender, age, systolic blood pressure, diastolic blood pressure, pulse, fasting blood glucose, alanine aminotransferase and uric acid levels, and logit model may serve as a satisfactory model for these types of study.

Abstract:Objective To investigate the feasibility and significance of joint microwave ablation for simultaneous treatment of two or more benign neoplasms originating from different organs which are conventionally managed in different disciplinary offices. Methods We treated a middle-aged female patient suffered with a large thyroid adenoma and a huge liver cavernous hemangioma with microwave ablation under local anesthesia and general anesthesia consecutively in one treatment session. The safety of operation and dynamic therapeutic effect were assessed. Results The thermal ablation procedures were both successful and eventful. The total operation time was about 90 minutes. The patient was discharged on the third day after ablation. During the follow-up, ultrasonography revealed a significant shrinkage of thyroid adenoma and liver hemangioma and recovery of the perinodular thyroid or liver tissue. The laboratory tests showed functional abnormalities of neither the thyroid, nor the liver and kidney. Conclusion: Joint microwave ablation for simultaneously treating large thyroid adenoma and huge liver cavernous hemangioma was verified safe, efficacious and medical resources sparing. This innovative therapeutic concept is worth of further investigation and promotion.

Abstract:A tibetan in-patient aged 43y suffered from ventricular tachycardia and heart failure was cheated with electrical cardioversion,and whose electrocardiogram (ECG)displayed typical type I Brugada wave from lead V1 to V3.Then his heart failure、secondary renal and hepatic inadequacy returened to normal gradually.Followed up one month after discharge,the patient had no complain,and there were type I Brugada wave in ECG as before and heart shadow shrunk in x-ray chest film.It is the first case report about the Brugada syndrome(Brs)in the tibetan.

Abstract: