Abstract:Objective To study the impact of autophagy inhibition on functions of dendritic cells (DCs) and mouse model of allergic asthma. Methods (1)BALB/c mice were divided into three groups using the random number table: asthma model group, asthma treated with autophagy inhibitor (chloroquine) group (asthma+CQ group) and control group. Mouse models in asthma group and asthma+CQ group were induced with ovalbumin (OVA); meanwhile, mice of asthma+CQ group were also treated with autophagy inhibitor CQ. Hematoxylin-Eosin (H-E) staining was used to observe the pathological changes in lung tissues of mice. ELISA, Western blotting analysis and flow cytometery were used to detect the serum OVA-specific IgE, autophagy level, and expression of surface co-stimulatory molecules and major histocompatibility complex class Ⅱ(MHC Ⅱ) on lung DCs, respectively. (2)Bone marrow-derived DCs were treated with autophagy inhibitor 3-methyladenine (3MA) in vitro and the surface expression of co-stimulatory molecules and MHC Ⅱ was detected. (3) We sorted CD4⁺ T cells from spleens of OT2 mice, then co-cultured with lung DCs from mice of different groups (T cells:DCs=1:10), and detected the activation and proliferation of T cells with flow cytometery. Results (1) The level of OVA-specific IgE (P<0.05), extent of inflammatory cell infiltration in lung tissues, autophagy level in lung DCs (P<0.05), and expression of CD86 and MHCⅡ (P<0.05)on lung DCs in asthma+CQ group were significantly lower than those in the asthma group. (2) 3-MA treatment decreased the surface expression of CD86 and MHCⅡ on bone marrow-derived DCs (P<0.05). And (3) lung DCs from asthma+CQ group had lower ability for activating T cells and promoting T cell proliferation than those from the asthma group (P<0.05). Conclusion Autophagy inhibitors can improve the pathologic condition of allergic asthma through inhibiting autophagy in DCs, down-regulating surface expression of co-stimulatory molecules and MHCⅡon DCs, and further inhibiting the DCs-induced proliferation of T cells.

Abstract:Objective To establish a novel transgenic mouse model of human PRKAG2 cardiac syndrome that overexpresses a PRKAG2-G100S mutation, so as to lay a foundation for further studying the role of human PRKAG2 gene in the development, morphology, and function of mouse heart. Methods Human PRKAG2 with G100S mutation was sub-cloned into a multiple cloning site located in the downstream of α-myosin heavy chain(α-MHC) promoter of the plasmid. After the construction of the transgenic expressing vector, C57BL/6J mice were selected as the genetic background, and the transgenic mouse model of PRKAG2-G100S mutation was built by microinjection. Genotype was further confirmed using specific primer PCR. Real time PCR and Western blotting analysis were used to examin the expression of human PAKAG2(G100S) mRNA and protein, respectively. Results Two strains of transgenic mice were successfully developed using backcross breeding, which specifically overexpressed the human PRKAG2-G100S mutation in the cardiac tissues of F2 generations by the methods qPCR and Western blotting at both mRNA and protein levels. Moreover, the PRKAG2-G100S mutation was successfully passed steadily. Conclusion We have successfully established a human PRKAG2-G100S transgenic mouse model, which can help to further explore the role of PRKAG2-G100S mutation in the development and function of mouse cardiac tissue in the PRKAG2-G100S cardiac syndrome.

Abstract:Objective To establish a method for fast construction of child growth curve by SPSS software. Methods The data of 17 721 children from Shanghai were extracted from the data base of a cross-sectional survey of children aged 0-7 years old in nine regions of China in 2005. Then SPSS software was used to calculate the mean, standard deviation, the 3^rd percentile (P₃), 10^th percentile (P₁₀), 50^th percentile (P₅₀), 90^th percentile (P₉₀) and 97^th percentile (P₉₇) of the boys' age specific body mass. The curve estimation function was used to select the appropriate fitting model and to draw the body mass centile curve. The property of curves was modified in order to achieve the best effect. The template invocation function was used to overlap the curves. Results Smooth and consistent growth curve of P₃, P₁₀, P₅₀, P₉₀ and P₉₇ for children in Shanghai were constructed through data calculation, curve estimation, curve fitting, template invocation and curve overlapping by SPSS software. Conclusion SPSS software can be used for fast construction of children's growth curves, and it has the advantages of convenient operation and good practicability, which makes it worthy of popularization.

Abstract:Objective To know about the Babesia infection situation in the blood donors in Guangxi, China, so as to provide a basical epidemical date, and to provide scientific proofs for safety blood transfusion. Methods A total of 1 900 peripheral blood samples were obtained from tubes of used blood bagstaking from Guangxi, China in 2013. Babesia spp. infections in the donors were tested using Babesia 18S rRNA and β-tubulin Nest-PCR, morphological observation, and indirect immunofluorescence assay (IFA). Results We found that the positive rate of Babesia infection was 2.53% (48/1 900) in Guangxi, with all cases caused by Babesia microti. The PCR sensitivity of Babesia 18S rRNA primer was much higher than that of β-tubulin. In 38 blood the erythrocytes were found to have circular and dense nuclear, with ring-form thin cytoplasm under microscope. The specific fluorescence was not observed when Nest-PCR positive specimens with IFA at ≥1:64 antibody concentration, and was taken as negative. Conclusion There is a certain proportion of Babesia microti infection in Guangxi. For those patients with immunocompromised condition who needs blood transfusion, Babesia microti should been tested in blood donors.

Abstract:Objective To prepare doxorubicin-loaded pH/magnetic dual responsive nanocomplex denoted as Fe₃O₄@SiO₂@PEG-b-PAsp@DOX and to determine its chemo-physical properties, pH/magnetic dual responsive release, and cytotoxicity against human lung cancer A549 cells. Methods The nanocomplex was synthesized through a sequential process involving hydrothermal treatment, Stber method, sol-gel technique, and cross-linking. The morphology, diameter, zeta potential and magnetic properties of the nanocomplex were characterized by transmission electron microscopy, zeta potential measurement analyzer, and hysteresis loop tester, respectively. Drug loading efficiency and encapsulation efficiency were examined by ultraviolet visible absorption spectroscopy; pH-stimulated drug release was investigated by dialysis in vitro; and the anti-proliferative activity apoptosis-induction effect of the complex nanoparticles were investigated by CCK-8 method and flow cytometer, respectively. Results The average particle size of drug-loaded system Fe₃O₄@SiO₂@PEG-b-PAsp@DOX was (197.7±1.5) nm and the zeta potential was (-35.9±0.6) mV. Drug loading efficiency and encapsulation efficiency were (20.36±0.67)% and (83.71±0.53)%, respectively. Cumulative release rate was significantly increased in mild acid condition (pH=5.5) (P<0.05). The nanocomplex also demonstrated a good magnetic response and targeting ability under outside magnetic field. Moreover, the drug-loaded nanoparticle showed a significant cytotoxicity effect against human lung A549 cells in vitro. Conclusion Fe₃O₄@SiO₂@PEG-b-PAsp@DOX possesses a good pH/magnetic dual responsive release characteristics and exhibits efficient antitumor activity in vitro against lung cancer A549 cells.

Abstract:Objective To investigate the protective effects of kaempferol against the fatty acid-induced islet microvessel endothelial function injury and the role of poly(ADP-ribose) polymerases-1(PARP-1). Methods Mouse islet microvessel endothelial MS-1 cells were divided into normal control group, solvent (DMSO) group, fatty acid group (0.25 mmol/L palmitic acid+0.5 mmol/L oleic acid), kaempferol group (50 μmol/L), fatty acid+kaempferol group, PARP-1 inhibitor (8 μmol/L BYK204165)+fatty acid group and PARP-1 inhibitors+fatty acid+kaempferol group. The changes of cell viability, apoptosis, nitric oxide (NO), nitric oxide synthase (NOS)and oxidative stress related indicators were examined in each group. Results After treatment with fatty acid, the survival rate of MS-1 cells was significantly decreased and the apoptosis rate was significantly increased (P<0.05); meanwhile, fatty acids also increased NO production and promoted the activities of the total NOS (tNOS), inducible NOS (iNOS) and constitutive NOS (cNOS) in the MS-1 cells (P<0.05). Treatment with fatty acid also significantly increased the lipid peroxidation products-malondialdehyde (MDA), while significantly decreased the levels of antioxidants, glutathione (GSH) and superoxide dismutase (SOD) (P<0.05); and it also increased the mRNA and protein expression of PARP-1, iNOS and cNOS (P<0.05). Kaempferol significantly attenuated the toxic effects of fatty acids concerning all the detected indicators (P<0.05). Moreover, pretreatment with PARP-1 inhibitor (BYK204165) for 1 h markedly enhanced the protective effects of kaempferol, and all the detected parameters were similar to those of the control group(P>0.05). Conclusion Fatty acid can directly trigger islet microvessel endothelial function injury, and kaempferol shows a protective effect against the toxicity of fatty acid. Inhibition of PARP-1 can significantly promote the protective effects of kaempferol.

Abstract:Objective To investigate the inhibitory effect of luteolin against proliferation of gastric cancer cell line MGC803 and the related mechanism. Methods The inhibitory effect of luteolin against MGC803 cell proliferation was evaluated by MTT assay and clone forming assay. The cell cycle changes were analyzed by the flow cytometry after staining propidium iodide (PI). Annexin Ⅴ-PI double staining was used to determine the apoptosis ratio of MGC803 cells. Bcl-2 family proteins, Caspase proteins, autophagy-associated and cell cycle-associated proteins were assessed by Western blotting analysis. Results MTT assay results showed that the IC₅₀ of luteolin against MGC803 cells for 24 h, 48 h and 72 h treatment were 127.37 μmol/L, 76.12 μmol/L, and 16.84 μmol/L, respectively. Luteolin treatment also inhibited cell colony formation of MGC803 cells. PI single staining flow cytometry found that cells were arrested in G₂ phase with the increase of luteolin concentration. Western blotting results showed that, with the increase of luteolin concentration, CDK4, CDK6 and CyclinE2 protein expression was decreased; CyclinD1, CyclinD3 and p-Wee1 protein kept unchanged; and CyclinA, CyclinB, Myt1 and p-cdc2 (Tyr15) protein expression was decreased. Flow cytometry Annexin Ⅴ-FITC/PI revealed increase of early and late apoptosis with the increase of luteolin concentration. Western blotting analysis showed that Mcl-1, Bcl-x_L, Caspase-3, Caspase-8 and Caspase-9 protein expression was decreased with the increase of luteolin concentration, with slightly changed Bcl-2 expression and increased Bax expression, which led to decreased Bcl-2/Bax, Mcl-1/Bax and Bcl-x_L/Bax ratios. At the same time, Caspase-3 and PARP also appeared in the strip cutting. The expression of P62 was decreased and expression of Beclin-1 and LC3 Ⅱ was increased. Conclusion Luteolin can arrest gastric cancer MGC803 cells at G₂ phase, inducing autophagy and inhibiting cell growth. Luteolin can also induce apoptosis of MGC803 cells via the mitochondrial pathway.

Abstract:Objective To apply meta-analysis for assessing the association between ABO blood group and diabetes mellitus risk by comparing the differences of ABO blood group distribution between diabetic patients and healthy control group. Methods We searched PubMed, CBM, VIP, CNKI and Wanfang Databases to collect studies about diabetes and ABO blood group. Eligible studies were selected according to the inclusion and exclusion criteria and related data were extracted. The Newcastle-Ottawa Scale was used to assess the quality of the included studies, and meta-analysis was performed by software Review Manager 5.1 and Stata 12.0. Results Blood group B and blood group AB had a different distribution among diabetic patients and the control group. Blood group B was more frequently seen in diabetic patients (OR=1.13, 95%CI: 1.05-1.21, P=0.000 7) and blood group AB was more frequently seen in healthy control group (OR=0.86, 95%CI: 0.77-0.97, P=0.01). But only in foreign populations did subgroup analysis showed a significant difference in blood group B and blood group AB distribution among the case and control group, but not in blood group A (OR=0.90, 95%CI: 0.76-1.07, P=0.24) or the blood group O distribution (OR=1.00, 95%CI: 0.89-1.13, P=0.96). Conclusion The occurrence of diabetes mellitus may be related to ABO blood group. In foreign populations, the risk of diabetes mellitus in B blood group subjects is higher than that of other blood groups, and blood group AB may be a protective factor for diabetes. Blood group A and blood group O are not associated with diabetes in domestic or foreign populations.

Abstract:Objective To prepare lappaconitine hydrobromide push-pull osmotic pump controlled release tablets and screen for the optimal formulation. Methods The percent of cumulative release was used as the evaluation index for the drug release profile in vitro. The effects of PEO N750, PEO WSR303, and plasticizer amounts and coating weight gain on the releasing behavior were investigated through single-factor method. Based on single-factor study on the compositions, the optimal formulation for lappaconitine hydrobromide push-pull osmotic pump controlled release tablet was selected via orthogonal design. The in vitro release of the optimized formulation was also fitted to different models. Results The results of orthogonal design indicated that coating weight gain had a significant effect on the drug release in vitro(P<0.05). The optimal formula was as follows: lappaconitine hydrobromide 20 mg, PEO N750 160 mg, NaCl 30 mg in drug layer; PEO WSR303 75 mg, NaCl 20 mg in the push layer; and plasticizer PEG 4000 was 10% and weight gain was 5% in the coating composition. The release rate of the tablets with optimized formulation was constant within 12 h, and the cumulative release could reach 95.02%. Conclusion The current method to prepare lappaconitine hydrobromide push-pull osmotic pump controlled release tablets is stable, and the in vitro drug release has an excellent zero-release profile within 12 h (r=0.992 1), which meets the standard for controlled release.

Abstract:Objective To determine the effect of Salvia przewalskii extract of total phenolic acids (SPE) on puromycin aminonucleoside (PAN)-induced oxidative stress in podocytes of rats in vivo and the effect of SPE on PAN-induced oxidative stress in podocytes of mice in vitro. Methods (1) Nephropathy rat model was established by PAN and was given intervention with SPE and tacrolimus.The renal tissue samples were obtained for WT1 staining to calculate the number of podocytes on the 5^th, 10^th, 15^th and 21^st day. The intensities of 8-hydroxy-2'-deoxyguanine (8-OHdG) were evaluated by immunofluorescence. (2)The podocytes of mice were exposed to PAN for 24 h in vitro, and then SPE, salvianolic acid B (SalB), rosmarinic acid (RA) or tacrolimus were added for 6, 12, 24, and 48 h culture. Then the cytoskeleton distribution of podocytes, indicated by F-actin, was observed by fluorescence microscopy, and the intracellular reactive oxygen species (ROS) production was measured by flow cytometry. Results (1)Decrease of podocytes per glomerular volume as measured by counting WT1-positive cells was started on day 5 in each group except normal control (NC) group, and on day 15 glomerular podocytes in PAN group was significantly less than that in the NC group ([14.4±0.7]/glomerular volume vs [37.2±1.5]/glomerular volume, P<0.05). The numbers of glomerular podocytes in SPE group and positive group (tacrolimus group) were more than that in PAN group at all time points. The glomerular podocyte count of high-dose SPE group was similar to that of positive group on day 15 ([21.7±1.0]/glomerular volume vs [23.6±1.2]/glomerular volume, P>0.05). After injection of PAN, 8-OHdG intensities were increased in each group except normal control group on day 5; and the intensities peaked on day 10 and then began to decrease, but still higher than that of the normal control group on day 15. The intensities of 8-OHdG in renal tissue was decreased after intervention, and those of the tacrolimus and high-dose SPE groups were similar. (2) In vitro study found that F-actin of podocytes was almost completely disrupted 24 h after PAN treatment, with disrupted filamentous structure. After the treatment with tacrolimus, SPE, SalB and RA, the PAN induced injury of podocytes was lessened, with reappeared polarity distribution of intracellular microfilaments. Compared with NC group, the ROS production in podocytes was significantly increased in PAN group (P<0.05).After treatment of podocyte with drugs, the ROS production was decreased. The cellular ROS production of positive control group was similar to those in tacrolimus group, low-dose SPE group, high-dose SalB group and RA group at 24 h. Compared with RA, SalB had a better efficacy in reducing ROS, and the reducing effect had a positive relation with drug dose. Conclusion Our study suggests that SPE can protect podocytes from PAN-induced oxidant stress in vivo and in vitro.

Abstract:Circular RNAs (circRNAs) are a species of RNAs with covalently closed loop structure and are widely expressed in eukaryocyte. Consisting of exons and/or introns, circRNAs are generated during the process of RNA splicing and are found to be relatively stable, evolutionally conserved and tissue/cell-specific. A few studies have indicated that circRNAs may participate in the RNA-RNA regulation network or RNA-protein complex formation and thus are involved in a diversity of diseases, including cancer. Here we reviewed the currently available outcomes and progress of researches concerning circRNAs and the potential relationship between diseases and circRNAs.

Abstract:Endometriosis is a common gynecological disorder of the reproductive age, and in recent years there is an increasing tendency in its incidence. Classical theories have failed to fully explain the exact pathogenesis of endometriosis. Previous studies have found the imbalance of oxidation system and antioxidant system and increased oxidation markers in patients with endometriosis, and it has also been found that antioxidants have prevention and treatment effects for endometriosis. All the above suggests that endometriosis is associated with oxidative stress. This article reviewed the correlation of endometriosis with oxidative stress, so as to provide reference for exploring the pathogenesis and possible antioxidant therapy of endometriosis.

Abstract:Survivin, a new member of inhibitors of apoptosis proteins(IAP) family, regulates the essential cellular processes, including inhibition of cell apoptosis, promotion of cell proliferation and tumor stromal angiogenesis. Survivin is undetectable in most terminally differentiated tissues, but upregulated in almost all types of human malignancies and its aberrant overexpression positively correlates with chemotherapy resistance, increased tumor recurrence and shortened patient survival. Because of its key role in tumor formation and development, Survivin is considered as an ideal target for anticancer treatment. This review discussed the molecular function of Survivin, relationship between Survivin and cancer biological characteristics, as well as the research progress of cancer therapy targeting Survivin.

Abstract:Objective To design and synthesize a new series of efficient, low toxicity azole derivatives using antifungal drug ketoconazole as the lead compound and to explore their anti-breast cancer activity. Methods Based on the docking mode of ketoconazole with estrogen receptor, We designed and synthesized eleven derivatives, whose 2, 4-dichlorophenyl and triazole ring were retained and the side chains were modified. Then the in vitro anticancer activities against breast cancer cells MDA-MB-231 and MCF-7 were determined by MTT using tamoxifene as the positive control drug. Results and Conclusion The synthesized compounds have been reported for the first time and they have been confirmed by ¹HNMR and ¹³CNMR. The synthesized azole derivatives have greater inhibitory effects than tamoxifene against breast cancer MDA-MB-231 cells.

Abstract:Objective To investigate the population dynamics of blood-sucking insects and detect the pathogen of rodents in Xisha Islands of China, so as to provide information for prevention and control of infectious diseases in the area. Methods From January to July in 2014, we collected blood-sucking insects in Yongxing and Shi Islands twice a month using light traps. The specimens were identified by morphologic characters and molecular markers. Meanwhile, the population size was calculated. The pathogenic infections of rodents were detected by immuno-colloidal gold chromatographic test strips and PCR assay. And the ectoparasites in the rodents were initially identified by morphologic characters. Results The dominant species of blood-sucking insects in Xisha Islands were species of Genus Culex, Armigeres and Culicoides, and in April the population density of biting midge was the highest (55.55%, 6 984/12 573). Phlebotomine sandfly specimen was also collected and identified as Sergentomyia baily (n=11) by mtDNA-COⅠ sequences. The positive rates of Staphylococcus aureus enterotoxin type A and Botulinum toxin type A were 3.45% (1/29) and 14.00% (7/50) in the rodent serum samples, while all the samples were negative for Tsutsugamushi disease and Plague antibodies. Sta58 gene of Rickettsia tsutsugamushi was amplified by nested PCR in 70 rodent's spleen tissues, with the positive rate being 11.43% (8/70). A total of 248 ectoparasites were collected, and 93.55% (232/248) of them was gamasid mites. Conclusion The population dynamics of blood-sucking insects has no obvious change. Phlebotomine sandflies has been first recorded in Xisha Islands. The infection rates by Rickettsia tsutsugamushi and two bacterial toxins are high.

Abstract:Objective To observe the preventive and therapeutic effects of scopolamine tablet and transdermal agent in combination with domperidone for seasickness in different voyage distances and sea state conditions. Methods A total of 236 participants were selected and batched for three trials (Trial 1: 80 sea miles voyage, 1-2 degree of sea state; Trial 2: 80 sea miles voyage, 3-4 degree of sea state; and Trial 3: 200 sea miles voyage, 3-4 degree of sea state). The motion sickness susceptibility questionnaire (MSSQ) was used to assess the seasickness susceptibility. The participants in each trial were divided into comprehensive medication group (CMG) receiving low dose oral and transdermal scopolamine in combination with oral domperidone, prophylactic medication group (PMG) receiving low dose oral and transdermal scopolamine, and placebo control group (PCG) receiving oral vitamine C. Motion sickness symptoms were evaluated by using Wiker rating scales. Results In trial 1, the Wiker scores and moderate seasickness incidence in the CMG and PMG were significantly lower than those in the PCG (P<0.05). No significant difference was observed in severe seasickness incidence rates among groups (P>0.05). In trial 2, the Wiker scores were significantly lower in the CMG than in the PCG (P<0.05). The incidence rates of severe seasickness were significantly decreased in CMG and PMG compared with PCG (P<0.05). No significant difference was observed in the moderate seasickness incidence rates among 3 groups (P>0.05). In trial 3, the Wiker scores were significantly lower in the CMG than in the PCG (P<0.05). The incidence rates of severe seasickness were significantly reduced in CMG and PMG compared with PCG (P<0.05). No significant difference was observed in the moderate seasickness incidence rates among 3 groups (P>0.05). Conclusion Low dose oral and transdermal scopolamine in combination with oral domperidone can alleviate seasickness symptoms and decrease incidence of severe seasickness during long-term voyage at sea.

Abstract:Objective To study the effects of hydrogen gas breathing on function recovery following clamp-induced spinal cord injury in mice. Methods A total of 36 male C57 mice were divided into 3 groups: sham group, spinal cord injury group and hydrogen treatment group (n=12 in each group). Animal model of clamp-induced spinal cord injury was made and a 66.7% hydrogen-4 times/day-3 days profile was given to animals in the hydrogen treatment group. The neuronal and motor function recovery following spinal cord injury was evaluated by general observation, BMS score, BBB score, and footprint analysis. Western blotting analysis was used to examine Caspase-3 protein expression; H-E and Nissl staining were also performed 7 days after the surgery. Results Compared with the spinal cord injury group, the BMS score and BBB score were significantly increased after hydrogen treatment (P<0.05). Moreover, Western blotting analysis showed that hydrogen treatment decreased apoptosis associated Caspase-3 protein expression; footprint analysis and histological examination also demonstrated great improvement. Conclusion Inhalation of hydrogen gas has a protective effect against clamp-induced spinal cord injury in mice, which may be a potential strategy for future application in clinical practice.

Abstract:Objective To investigate the epidemiology and causes of skin diseases in soldiers stationed in south-east coastal areas of China, and to put forward measures for prevention and treatment. Methods Multi-stage sampling including cluster sampling, stratified sampling and simple random sampling were used to select 625 soldiers stationed in the south-each coastal areas of China. They were surveyed by a questionnaire and received physical examination for skin diseases before diagnosing and prescribing. Logistic multiple regression analysis was used to analyze the relationship between skin disease and the possible causes of skin diseases. Results A total of 569 effective questionnaires were collected, and the participants ranged in age from 17-46 years old. It was found that 263(46.40%) soldiers had skin diseases, which fell into 40 types, with a total of 346 onsets, mainly including infectious skin diseases(197 onsets, 56.94%), allergodermia(80 onsets, 23.12%)and psychosomatic skin diseases(33 onsets, 9.54%).And the causes of the disease were complicated. Conclusion Skin diseases among soldiers stationed in south-east coastal areas of China are associated with the climate, environment, dress and equipment, and mental condition. More efforts should be made for a better living condition and habit, scientific military training, good knowledge of skin disease prevention, and more careful medical service to reduce skin diseases among the soldiers.

Abstract:Objective To explore the morbidity and possible mechanism of spinal cord ischemia (SCI) after infrarenal abdominal aortic aneurysm (IAAA) endovascular aneurysm repair (EVAR). Methods We retrospectively analyzed the intra-operative hypogastric artery occlusion and postoperative SCI of 400 patients who received EVAR in the Departments of Vascular and Endovascular Surgery of Shanghai Changhai Hospital and Changzheng Hospital from January 2008 to October 2014. The morbidity and possible mechanism of SCI after EVAR were analyzed while combining the existing literatures. Results Bilateral hypogastric arteries were obstructed during operation in 60 patients (unilateral hypogastric artery aneurysms were embolized by spring coil in 8 cases); unilateral hypogastric arteries was obstructed in 70 patients (unilateral hypogastric artery aneurysms were partially embolized by spring coil in 10 cases). Postoperatively 2 cases had acute lower limb artery ischemia, 1 had acute SCI, and 1 had chronic lower limb lameness(> 3 months). The incidence of SCI was 0.25%(1/400). Existing literatures showed that the incidence of SCI following EVAR was 0.21%-0.38%, and only 1 of the 14 cases with SCI was thought to be associated with the hypogastric artery's interruption. Conclusion SCI is a very rare postoperative complication of EVAR, with the mechanism remaining unknown. The occlusion of hypogastric artery may play a part, but existing literatures suggest a non-core role. In addition to ischemia caused by SCI and embolization, the perioperative general condition of patients also needs to be taken into consideration.

Abstract:Objective To address the levels of serum miR-21/155/181a/18b in dilated cardiomyopathy (DCM) patients at a progressive heart failure stage. Methods The serum miRNAs were measured by real-time reverse-transcription PCR in 40 DCM patients and in 30 healthy controls. Pearson's correlation coefficient was used to calculate the potential correlation between miRNAs and other indicators, such as human N-terminal pro-brain natriuretic peptide (NT-proBNP), high-sensitivity C-reactive protein (hsCRP), left ventricular ejection fraction (LVEF%), and left ventricular end-diastolic diameter (LVEDD). Results Our findings revealed that serum miR-21 were significantly reduced in DCM patients compared with the healthy controls(P<0.01). We also found that serum miR-21 was positively correlated with LVEF% and negatively correlated with LVEDD (P<0.001). Conclusion The serum level of cardiac remodeling-associated miR-21 is decreased in DCM patients at a progressive heart failure stage compared to the healthy controls, which may be also related to the disease severity.

Abstract:Objective To explore the effects of physical activity (PA) on lipid profile and identify the optimal intensity of PA that improves dyslipidemia. Methods Community-based individuals aged 40-60 years old were recruited by a cluster sampling method. PA was estimated using the International Physical Activity Questionnaire, and PA levels of individuals were classified as low, moderate, or high. Lipid profiles were measured by using fasting blood samples, and the potential confounding variables related to lipid profiles were comprehensively collected. Unconditional logistic regression method was used to investigate the relationship of PA with lipid profiles. Results A total of 5 664 subjects (38.37% men) were included in this study. The percentages of individuals with low, moderate and high PA levels were 9.99% (566/5 664), 44.79%(2 537/5 664) and 45.22%(2 561/5 664), respectively. High PA could reduce the risk of dyslipidemia, with an odds ratio (OR) of 0.83 (95% confidence interval [CI]: 0.71-0.98) for dyslipidemia compared to low PA, while the association was not found for moderate PA. High level PA mainly influenced the high-density lipoprotein cholesterol and triglycerides. Conclusion High level PA, compared with low level, can reduce the risk of dyslipidemia in middle-aged Chinese population.

Abstract:Objective To investigate the sleep quality of students from a medical university and to analyze the risk factors of sleep disorders. Methods Students from a medical university were selected with cluster sampling method. A total of 886 students were included in this study: there were 55 Major A students, 80 Major B students, 97 Major C students, 577 Major D students, and 77 Major E students. The students were investigated by Pittsburgh sleep quality index (PSQI) and self-designed physical state questionnaire. Results (1)The number of effective questionnaire was 843 and sleep disorders were found in 284 students, with an incidence of 33.7% and a PSQI score of 5.48±0.12. The incidences of sleep orders and PSQI scores were 43.4% (23/53) and 6.23±0.48 for Major A, 32.9% (23/70) and 4.93±0.44 for Major B, 40.4% (36/89) and 5.75±0.34 for Major C, 32.4% (183/564) and 5.47±0.14 for Major D and 28.4% (19/67) and 5.18±0.40 for Major E, with no significant difference found for different majors (P>0.05). (2)The factors leading to somnipathy included emotional stress, ambient noise, somatic pain, school learning, seldom late-night snack and occasional late-night snack (OR=0.577, 0.611, 0.265, 0.260, 0.547, and 0.507, respectively). Conclusion The students in the involved medical university have a low sleep quality which may affect their physical state during day time. Active measures should be taken to improve students sleep quality so as to insure their study.

Abstract:

Abstract:epithelioid hemangioendo thelioma:A Case Report and Review of Literature

Abstract:Lipoma is a common benign tumor composed of mature adipocytes posed,which is mostly in the fat layer. It is the fat tissue nodules and can be found anywhere in the body, but the limbs, trunk is common, followed by retroperitoneal and gastrointestinal wall places. Intraperitoneal and intramuscular lipoma is relatively rare, moreover,the large intra-abdominal and leg muscles simultaneously is even rarer, now we are report a case that a giant lipoma between the abdominal cavity and thigh muscle in the Second Affiliated Hospital of Nanchang University in 2015 .The clinical manifestations , treatment and prognosis of the disease will be summarized in this report.