Abstract:Traditional Chinese Medicine has its characteristics and advantages in the comprehensive prevention and treatment of malignant tumors. Based on long-time clinical practices and experimental studies, Department of Traditional Chinese Medicine of Changzheng Hospital of Navy Medical University (Second Military Medical University) established that "phlegm" is the core pathogenesis throughout the whole process of gastric cancer, and developed a theory of "phlegm syndrome of gastric cancer" and a series of prescriptions for eliminating phlegm. The therapeutic regimen based on this theory can significantly improve the quality of life and immune function and prolong the survival of patients with gastric cancer. This review introduces the theory of "phlegm syndrome of gastric cancer" and the treatment effect of eliminating phlegm prescriptions and drugs, elucidates their possible molecular mechanisms from the aspects of gastric cancer cell proliferation, apoptosis, epigenetic abnormalities, metastasis and tumor microenvironment, hoping to deepen the understanding of traditional Chinese medicine in comprehensive treatment of gastric cancer.

Abstract:Objective To explore the effect of hepatitis C virus (HCV) infection on acetylcholinesterase (AchE) expression level and the effect of AchE activity on the infection of HCV. Methods Human hepatoma cell line Huh7 cells were infected with cell culture-derived HCV (HCVcc). AchE expression level was analyzed using Western blotting and real-time quantitative PCR (qPCR). AchE enzyme activity was assayed using AchE activity detection kit. Huh7 cells were treated with AchE inhibitor, donepezil or itopride, together with HCVcc infection, and then the HCV infection level of Huh7 cells was detected by Western blotting and immunofluorescence. Huh7 cells were transfected with small interfering RNA (siRNA) targeting to AchE gene, followed by infecting with HCVcc, and then the expression levels of AchE and HCV infection were analyzed by Western blotting and immunofluorescence. Results After 60 h of HCVcc infection, the expression level of AchE protein in Huh7 cells and the AchE activity were significantly increased (both P<0.01). AchE inhibitors significantly inhibited HCV infection of Huh7 cells in a concentration-dependent manner (P<0.01). After knocking down AchE with siRNA, HCV infection was significantly inhibited (P<0.01). Conclusion HCV infection of Huh7 cells can up-regulate the expression level of AchE and enhance the activity of AchE. The increase of AchE expression and activity can promote HCV infection, indicating that AchE plays a positive feedback enhancement effect on HCV infection of Huh7 cells.

Abstract:Objective To explore the relationship between long non-coding RNA (lncRNA)-8439 and pluripotency factor nanog, and to investigate the potential effect of lncRNA-8439 on the growth of hepatoma carcinoma cells. Methods RNA-sequencing and bioinformatics tools were used to screen the differentially expressed lncRNA related to pluripotency factors (nanog, oct4 and sox2) in primary and metastatic tissues of hepatocellular carcinoma. The differentially expressed lncRNA levels were tested by real-time quantitative PCR (qPCR) in human hepatoma carcinoma cell lines, Hep3B and Huh7, and their corresponding suspended spheres. The potential of binding of lncRNA-8439 and nanog was determined using bioinformatics tools. Localization of lncRNA-8439 in the Hep3B and Huh7 cells was observed by fluorescence in situ hybridization. After knocking down lncRNA-8439, the expression levels of nanog in the Hep3B and Huh7 cells were detected by qPCR and Western blotting, and the growth status of suspended spheres was observed. After overexpressing lncRNA-8439, the expression levels of nanog were measured by qPCR and Western blotting in the two kinds of cancer cells. Results A total of 9 differentially expressed lncRNAs related to pluripotency factors were identified, while only lncRNA-8439 expression was significantly higher in the suspended spheres of two kinds of cancer cells compared with their corresponding adherent cells (both P<0.01). LncRNA-8439 had binding sites of nanog at its 3'end. LncRNA-8439 was located in the nucleus of the Hep3B and Huh7 cells but not in the cytoplasm. After knocking down lncRNA-8439, the RNA and protein expression levels of nanog were significantly reduced in the Hep3B and Huh7 cells (all P<0.01), and the number of suspended spheres was also reduced. After overexpressing lncRNA-8439, the RNA and protein expression levels of nanog were significantly up-regulated in the two kinds of cancer cells (all P<0.01). Conclusion LncRNA-8439 affects self-renewal ability of hepatoma carcinoma cells through up-regulating expression of pluripotency factor nanog, which may be the cause of invasion and metastasis of hepatocellular carcinoma.

Abstract:Objective To determine the expression level of BICD cargo adaptor 1 (BICD1) gene in different grades of glioma in Chinese population, and to explore its potential role in progress of low-grade glioma to high-grade. Methods The BICD1 mRNA expression data were derived from whole transcriptome expression profile array database and whole transcriptome sequencing database of Chinese Glioma Genome Atlas (CGGA). The information of World Health Organization (WHO) grade, molecular subtype, progression-free survival, overall survival, and expression levels and mutation status of typical molecular biomarkers were also collected from the database. The correlation of BICD1 expression with progression and malignancy grade was analyzed. Ten samples of glioma of WHO Ⅱ, Ⅲ and Ⅳ grade were collected, and the extracted RNA was reverse transcribed into cDNA. Then real-time quantitative PCR (qPCR) was performed to analyze BICD1 expression levels in each grade. Results Expression levels derived from whole transcriptome expression profile array database of CGGA and whole transcriptome sequencing database of CGGA showed that BICD1 expression level was significantly increased with WHO grade of glioma (WHO Ⅲ and Ⅳ grade vs WHO Ⅱgrade:t=7.901, P<0.01). qPCR analysis showed that BICD1 expression level was significantly higher in the glioma of high-grade versus the glioma of low-grade (WHO Ⅲ grade vs WHO Ⅱ grade:t=3.514, P<0.01; WHO Ⅳ grade vs Ⅲ grade:t=2.128, P=0.037 6). High expression of BICD1 was significantly correlated with shorter progression-free survival and overall survival of the glioma patients (both P<0.01). The BICD1 expression levels were significantly different between the proneural, neural, classical and mesenchymal gliomas (F=21.8, P<0.01), and the BICD1 expression level in the mesenchymal glioma was the highest and the one in the proneural glioma was the lowest. The BICD1 expression level was correlated with classical molecular markers of malignant degrees of glioma, such as isocitrate dehydrogenase 1 (IDH1) mutation, combined deletion of chromosome 1p19q and phosphatase and tensin homolog deleted on chromosome ten (PTEN) mutation. The expression level of BICD1 was significantly lower in the IDH1 mutation glioma samples than that in the wildtype samples (t=7.769, P<0.01). Conclusion BICD1 may be a potential biomarker for grade progress and malignant progression of low-grade glioma.

Abstract:Objective To establish a New Zealand white rabbit model of diving decompression sickness and an evaluating system for the model. Methods Twenty-five New Zealand white rabbits were subjected to a simulated dive in DWC150 animal compression chamber. After 60 min exposure to compressed air at 500 kPa, the pressure inside the chamber was decreased to normal pressure at a constant speed of 200 kPa/min. Six healthy New Zealand white rabbits were ventilated under normal pressure and set as normal control group. After decompression, the amount of gas bubbles flowing through the right ventricle was observed by ultrasonography. The amount of gas bubbles, functional status of hind limbs, respiratory function, and lung and spinal cord pathological examination results were scored, and the blood routine and coagulation function were detected. Results With this decompression program, the incidence of decompression sickness was 76% (19/25) and mortality rate was 28% (7/25). Cardiac ultrasonography showed great amount of gas bubbles in venous system of the rabbits with decompression sickness, and the gas bubble score was significantly higher than that in the normal control group (Z=-3.702, P=0.002). The rabbits with decompression sickness had abnormal hind limb motor function and respiratory function, and the Tarlov score and respiratory function score were significantly higher than those in the normal control group (Z=-2.172 and -3.702, both P<0.05). The rabbits with decompression sickness had significantly higher lung wet/dry dry mass ratio versus the normal control rabbits (t=4.52, P<0.01). H-E staining showed that the rabbits after decompression for 24 h had alveolar hemorrhage, alveolar septum thickening and vacuole-like changes of spinal cord. The white blood cell counts of the rabbits significantly increased at 6 h and 12 h after decompression (t=3.933, 2.838; P=0.003, 0.019), and the red blood cell count and hematocrit significantly decreased at 1 h after decompression (t=-2.606, -2.481; P=0.031, 0.038). After decompression, the platelet count first increased and then decreased, and the difference was significant (F=3.024, P=0.039); and the platelet count at 12 h after decompression was significantly higher than that before decompression (t=2.545, P=0.031). Conclusion We have successfully established a rabbit decompression sickness model and a set of evaluating parameter system considering the overall behavior, histopathology of lung and spinal cord tissues, inflammatory response and coagulation tests.

Abstract:Objective To explore the effects of preoperative rosuvastatin and atorvastatin on postoperative renal function in elderly patients undergoing coronary angiography. Methods From Jan. 2018 to Jun. 2018, 345 patients undergoing coronary angiography in Department of Cardiovasology of Changhai Hospital of Navy Medical University (Second Military Medical University) were divided into rosuvastatin group (n=171) and atorvastatin group (n=174). The patients in the two groups were further divided into <70 years subgroup and ≥ 70 years subgroup. All patients continued to take the original routine medications and routine dose of statins before bedtime. The serum creatinine levels were measured before and within 72 hours after coronary angiography. The incidence of contrast-induced nephrology was analyzed in each group. Results The postoperative serum creatinine level of the patients in the rosuvastatin group was (99.2±28.9) μmol/L, which was higher than that before operation ([92.2±29.5] μmol/L); and the difference was statistically significant (t=4.734, P<0.01). There were no significant differences in the serum creatinine levels before and after operation in the patients of the atorvastatin group (P>0.05). The postoperative serum creatinine level was significantly higher than that before operation in the rosuvastatin group, regardless of the age of <70 or ≥ 70 years ([88.0±15.6] μmol/L vs[82.3±13.5] μmol/L, t=3.858, P<0.01;[104.8±32.2] μmol/L vs[97.2±33.7] μmol/L, t=3.657, P<0.01). The postoperative serum creatinine level was significantly higher than preoperative one in the patients aged <70 years in the atorvastatin group ([128.3±44.4] μmol/L vs[122.4±43.4] μmol/L, t=2.854, P=0.006), but the postoperative and preoperative serum creatinine levels were not significantly different in the patients aged ≥ 70 years (P>0.05). The incidence of contrast-induced nephrology was significantly higher in the rosuvastatin group than that in the atorvastatin group (21.1%[36/171] vs 6.9%[12/174], χ²=14.430, P<0.01). And the incidence of contrast-induced nephrology in the patients aged ≥ 70 years was significantly higher than that in the patients aged <70 years of the rosuvastatin group (28.9%[33/114] vs 5.3%[3/57], χ²=12.825, P<0.01). Conversely, there was no significant difference in the incidence of contrast-induced nephropathy between the patients aged ≥ 70 years and <70 years in the atorvastatin group (P>0.05). Conclusion Compared with rosuvastatin, atorvastatin has a more potent effect on protecting renal function and reducing the incidence of contrast-induced nephrology in elderly patients after coronary angiography, and this advantage is more obvious in the patients aged ≥ 70 years.

Abstract:Objective To explore the association between single nucleotide polymorphism (SNP) of apolipoprotein M (apoM) gene and chronic obstructive pulmonary disease (COPD), so as to provide evidence for screening early diagnosis and treatment of COPD in high-risk population. Methods The base-quenched probe technique was performed to determine and analyze the apoM gene SNP loci (rs805264, rs707922 and 707921) of 256 COPD patients (COPD group) and 248 healthy control participants (control group). Results The frequencies of apoM (rs805264, rs707922 and rs707921) genotypes of COPD patients and healthy control participants were found to be in genetic equilibrium according to the Hardy-Weinberg Law (all P>0.05). Compared with the control group, the frequency of rs805264 locus AA genotype was lower and the frequency of GG+GA combined genotype was higher in the COPD group, and the difference was significant (χ²=4.769, P=0.029). Compared with the control group, the frequency of rs707921 locus AA genotype was lower and the frequency of CC+CA was higher in the COPD group, and the difference was significant (χ²=4.769, P=0.029). However, there was no significant difference in the genetype frequency distribution of rs707922 locus between the two groups (P>0.05). There were no significant differences in the frequencies of alleles of apoM rs805264, rs707922 or rs707921 loci between the COPD and control groups (all P>0.05). The rs805264 and rs707921, and rs707922 and rs707921 showed strong linkage disequilibrium (both D'>0.8, both r²>0.8); and rs805264 and rs707922 showed complete linkage disequilibrium (D'=1.000, r²=0.820). Conclusion ApoM gene rs805264 and rs707921 loci may be associated with COPD susceptibility, while the rs707922 locus may not be associated with COPD.

Abstract:Objective To explore the possible causes and the appropriate management of liquefactive necrosis in ablative area after microwave ablation for thyroid nodules. Methods A total of 3 480 thyroid nodules treated with microwave ablation between Jan. 2008 and Dec. 2017 were followed up. The clinical data, treatment methods and outcomes of the patients with liquefactive necrosis in ablative area were analyzed retrospectively. Results Of the 3 480 thyroid nodules, 18 (0.52%) had liquefactive necrosis in ablative area after microwave ablation. The maximum diameters of the 18 (100.00%) thyroid nodules were all greater than or equal to 2.5 cm before microwave ablation, the locations were all closely adjacent to the thyroid capsule, blood flows surrounding the nodules were all blocked by microwave energy during ablative operation, and the counts and proportions of white blood cell in the peripheral blood were all normal after the occurrence of liquefaction. Eighteen patients with liquefactive necrosis in ablative area were all clinically cured. In 2 cases among them, the necrosis dissipated spontaneously after orally taking anti-inflammatory drugs without local treatment for the ablation area. Ten patients underwent surgical incision to remove the liquefactive necrotic substance in the ablation area, and the incision healed on 14-26 days; and 2 cases in them had obvious scar formation on the neck skin. Six patients underwent ultrasound-guided drainage to remove the necrosis, the subcutaneous sinus tract healed on 10-20 days, and all had no neck scar formation. Bacterial culture of liquefactive material was performed in 16 patients, and the results were all negative. Conclusion The liquefaction of coagulated necrotic tissue in the ablative area of thyroid nodules after microwave ablation is a sterile process, and may be related to the large size of nodules, the close location of nodules to the capsule and the thermal blockade of blood flow. The "liquefaction and absorption disequilibrium" hypothesis may provide clues for exploring its potential mechanism. Ultrasound-guided drainage by dilating the subcutaneous sinus can successfully remove the necrotic materials and avoid scarring of neck skin.

Abstract:Objective To explore the relationship between the first-phase insulin secretion and diabetic microvascular complications in patients with type 2 diabetes mellitus (T2DM) using arginine stimulating test. Methods Sixty-seven patients with T2DM, aged (56.4±14.2) years, were enrolled from the Affiliated Hospital of Nantong University, and they included 42 males and 25 females. The patients with microvascular complications were assigned to case group (n=23), and the patients without microvascular complications were assigned to control group (n=44). The parameters, including height, body mass, duration of diabetes, renal function, plasma lipids, urinary microalbumin, and urine creatinine, were collected. Body mass index (BMI), urinary microalbumin/creatinine ratio (UACR) and estimated glomerular filtration rate (eGFR) were calculated. The arginine stimulating test was performed in the fasting state. The levels of plasma glucose, and serum insulin and C-peptide were tested in the fasting state, and at 2, 4 and 6 min after injecting arginine. The acute insulin response (AIR) index, acute C-peptide response (ACR) index, area under curve of insulin (INS_AUC) and homeostasis model assessment of insulin resistance (HOMA-IR) index were calculated, and their correlations with diabetic microvascular complications were analyzed. Results The duration of diabetes of the T2DM patients in the case group was significantly longer than that in the control group (P=0.030). Compared with the control group, the serum creatinine level, UACR, serum insulin and C-peptide levels (in the fasting state and at 2, 4, 6 min after injecting arginine), AIR index, INS_AUC, and HOMA-IR index were higher, and the eGFR was lower in the case group, and the differences were significant (all P<0.05). The logistic regression analysis after adjusting for age and BMI showed that the duration of diabetes and AIR index were significantly correlated with the diabetic microvascular complications (odds ratio[OR]=1.099, 95% confidence interval[CI] 1.011-1.194, P=0.026; OR=1.049, 95% CI 1.007-1.092, P=0.021). Conclusion The diabetic microvascular complications are associated with the long duration of diabetes and high AIR index in the patients with T2DM.

Abstract:Objective To establish an ultra high performance liquid chromatography-tandem quadrupole mass spectrometry (UPLC-MS/MS) method for simultaneous determination of 5-hydroxytryptamine (5-HT) and 5-hydroxyindoleacetic acid (5-HIAA) in cerebrospinal fluid of depressive rats, and to investigate the concentrations of 5-HT and 5-HIAA in cerebrospinal fluid of depression rats and fluoxetine treated rats. Methods Twenty-four SD rats were randomly divided into control group, model group and fluoxetine group, with 8 rats in each group. At 30 d of model building, the cerebrospinal fluids were taken after the test of behaviors, and the concentrations of 5-HT and 5-HIAA were determined by UPLC-MS/MS after pretreatment of protein precipitation. Chromatographic column was ACE C₁₈-PFP column (4.6 mm×150 mm, 3 μm), mobile phase was acetonitrile and 0.2% formic acid-water solution, flow rate was 0.4 mL/min, and the column temperature was 25℃. The mass spectrometry detection was performed using dynamic multiple reaction monitoring (DMRM). Results 5-HT and 5-HIAA in cerebrospinal fluid of depression rats had good linear relationship within the concentration range of 0.10-100.00 ng/mL and 1.00-1 000.00 ng/mL, respectively (r>0.999). Intra-and inter-day precision was less than 15.00%; the recoveries ranged from 86.86% to 98.49%; and the matrix effects ranged from 89.80% to 95.25%. The samples remained stable during analysis. Compared with the control group, the concentration of 5-HT in cerebrospinal fluid of rats in the model group was significantly decreased (P<0.01), and the concentration of 5-HIAA was significantly increased (P<0.01). Compared with the model group, the concentration of 5-HT in cerebrospinal fluid of rats in the fluoxetine group was significantly increased (P<0.01), and the concentration of 5-HIAA was significantly decreased (P<0.01). Conclusion The UPLC-MS/MS method is simple, accurate, sensitive, reproducible and suitable for simultaneous determination of 5-HT and 5-HIAA in cerebrospinal fluid of depression rats.

Abstract:Streptozotocin (STZ) has been widely used to create diabetic retinopathy (DR) model in a variety of animals. The clinical features and pathological changes of the animal models of DR induced by STZ are similar to those of DR of human type 1 diabetes mellitus, so the animal models are frequently used to study the pathogenesis of DR and evaluate new pre-clinical anti-DR drugs. Mice are one of the best models to study DR because of small size, low cost, ease of operation and high success rate of induction. However, researchers also face many challenges in inducing mouse model of DR, such as reproducibility of the model and animal lethality by STZ. In the process of inducing animal DR model by STZ, we need to pay attention to several key factors, including the preparation of STZ, suitable dosage, route of administration, feeding method, and age, body mass and gender of the animals. This review analyzes the mechanism of STZ-induced DR, emphasizes the important processes of STZ-induced mouse DR model, and proposes the methods to reduce the lethality of STZ.

Abstract:Chronic rhinosinusitis (CRS) is a chronic inflammatory disease of the nasal cavity and paranasal sinuses, and the pathological manifestations include mucus hypersecretion and mucosal inflammation induced by various inflammatory mediators. Macrolide antibiotics are widely used in the clinical treatment of CRS due to the anti-inflammatory and immunomodulatory effects. A low-dose, long-term oral macrolide antimicrobial method is recommended, and it is effective for non-allergic CRS without nasal polyps or eosinophilia, or with normal immunoglobulin E level. This paper reviews the mechanism, pharmacological characteristics and clinical application of macrolide antibiotics for CRS.

Abstract:Adipose-derived mesenchymal stem cells (ASCs) are a group of mesenchymal stem cells derived from adipose tissue and can differentiate into multiple lineages cells, including osteoblasts, chondrocytes, adipose cells and so on. ASCs have the advantages of abundant source, easy access to materials and low damage in clinical application. Researches discover that ASCs do not express histocompatibility complex (MHC) Ⅱ and costimulatory molecules, and have poor immunogenicity, which provides the possibility for the application of allogenic ASCs. Furthermore, ASCs have immunomodulatory effects on several cytokines through inhibiting T lymphocyte activation, inhibiting B lymphocyte apoptosis, and promoting macrophage differentiation to immunoregulatory phenotype. The roles of ASCs are stable in hypoxia, inflammatory environment and low-serum medium. Considerable literature shows that allogenic ASCs are safe and efficient in animal experiments and clinical trials without severe complications, and they are expected to play important roles in the treatment of autoimmune diseases, improving the function of degenerative tissues and induction of immune tolerance in allograft. This paper reviews the immunomodulatory mechanisms and the roles of ASCs in autoimmune diseases, graft rejection, and tissue regeneration and repair.

Abstract:Objective To optimize in situ hybridization (ISH) method for microRNA (miR) detection in formalin-fixed and paraffin-embedded (FFPE) pancreatic cancer tissue sections, so as to improve the sensitivity of miR detection. Methods Tissue microarray (TMA) was constructed from 20 pancreatic cancer tissue specimens. Using locked nucleic acid (LNA) labeled probe, we examined the expression levels of miR-21 and miR-34a by chromogenic in situ hybridization (CISH). The hybridization temperatures were set at 48, 53 and 56℃, respectively. Three different washing approaches (temperature, time and 3 different washing buffers) were adopted to optimize the hybridization conditions. TMA was prepared using 126 pancreatic cancer tissue specimens, and optimal CISH was used to detect the expression levels of miR-21 and miR-34a. Results The optimal hybridization conditions of miR-21 probe were as follows:hybridization at 53℃ for 6 h, washing with washing buffer Ⅲ at 65℃ for 6 min, and then washing with PBS for 1 min. The optimal hybridization conditions of miR-34a probe were as follows:hybridization at 53℃ for 6 h, washing with washing buffer Ⅲ at 4℃ and 65℃ for 6 min, respectively, and then washing with PBS for 1 min. Of 126 pancreatic cancer tissue specimens, 84 (66.7%) had positive expression of miR-21, and 77 (61.1%) had positive expression of miR-34a. Conclusion The optimal hybridization temperatures of miR-21 and miR-34a are both 53℃ in the FFPE pancreatic cancer tissue sections, and appropriate washing temperature and washing buffer after hybridization are conducive to improve the positive detection rate of miR.

Abstract:Objective To explore the application value of virtual reality training in urethrovesical anastomosis of robot-assisted laparoscopic radical prostatectomy. Methods Using robot simulated training system, 3 surgeons were trained by anastomotic simulation training for basic operation. The key parameters, including overall score, time to complete anastomosis, robot movement distance, instrument collision times, times of instrument out of sight and missed target times, were compared before and after training. The 3 surgeons carried out urethrovesical anastomosis in robot-assisted laparoscopic radical prostatectomy for 9 patients using robot-assisted system. The quality of anastomosis was evaluated. Results After training, the overall score of 3 surgeons was increased from 65.0±10.8 before training to 92.7±3.5, time to complete anastomosis was shortened from (279.0±48.0) s to (119.3±12.5) s, and robot movement distance was increased from (459.0±59.2) cm to (239.3±33.9) cm; and the differences were significant (all P<0.05). There were no significant changes in the times of instrument out of sight or missed target before and after training. The urethrovesical anastomosis of 9 patients were successfully performed by the 3 surgeons during robot-assisted laparoscopic radical prostatectomy, with the average anastomosis time being (23.4±8.6) min. No increased creatinine level was found in drainage fluid of the patients and no leakage of urine occurred. No leakage of contrast agents was found in cystography on the 7^th day after operation, and the catheter was removed on the 8^th day after operation. Conclusion Virtual reality training using robot simulated system can help urologists to get familiar with the robotic system quickly. It can improve the urethrovesical anastomosis during operation with high efficiency and quality.

Abstract:Objective To evaluate the reliability and validity of SF-36 scale for life quality assessment of naval service personnel, and to understand the status and influencing factors of health-related quality of life. Methods The SF-36 scale was used to evaluate the quality of life of the naval service personnel in a regional military hospital in 2016. Results A total of 180 questionnaires were sent out, 162 questionnaires were recovered and 149 valid questionnaires were left for analysis. SF-36 scale had good internal-consistency reliability and split-half reliability, and had good convergent validity and discriminate validity. The males scored lower than the females in three dimensions, including bodily pain, social functioning and role-emotional, and the differences were significant (all P<0.05). Compared with the males in the same age group of national survey, the males of this survey scored significantly lower in physiological functioning, role-physical, bodily pain, social functioning and role-emotional, and scored significantly higher in general health (all P<0.05). The females of this survey scored significantly higher in general health and role-emotional than the females of the national survey (both P<0.05). Correlation analysis showed that having chronic disease, the quality of sleep, gender and physical discomfort in the last 2 weeks were the main influencing factors in each dimension of SF-36 scale. Conclusion The SF-36 scale can be used for evaluating the health-related quality of life of the naval service personnel, and improving the quality of sleep, controlling chronic diseases and reducing the short-term physical discomfort can help to improve the quality of life of naval service personnel.

Abstract:Objective To explore the application of inductive classification algorithm based on decision tree in optimization of medical treatment process. Methods Taking the test results of the burn inpatients as general data, we used inductive classification algorithm based on decision tree for medical treatment process optimization with medical treatment efficiency as the target. The model of decision tree was constructed and the rules for the optimization of medical treatment process were excavated. Results Among 10 pathological attributes, extent of burn, blood biochemistry, blood pressure and pulse played key roles in determining the patient treatment program after optimizing decision tree process. When the burn was mild, the treatment plan could be determined only by examining blood biochemistry indexes. When the burn was moderate, the treatment plan could be determined first by examining blood biochemistry indexes and then by examining blood pressure or pulse. When the burn was severe, emergency treatment plan should be adopted directly. Conclusion Data mining technology represented by decision tree can contributes to differential diagnosis of burn and optimization of the treatment process.

Abstract:Objective To investigate the relationship between symptoms of post-traumatic stress disorder (PTSD) and the attachment patterns among parents who lost their only child. Methods A total of 149 individuals who lost their only child were randomly selected in an area of Shanghai, China. Post-traumatic stress disorder check list-civilian version (PCL-C) scale and adult attachment scale-revised edition (AAS-1996) were used to assess the symptoms of PTSD and adult attachment patterns. Results The prevalence rate of PTSD was 30.9% (46/149), and the prevalence rate of the females was significantly higher than that of the males (P<0.01). The prevalence rate of PTSD of the individuals with insecure attachment patterns was significantly higher than that of the individuals with secure attachment patterns (P<0.01), and the prevalence rates were significantly different between the two groups in the B (repeated traumatic experience), C (emotional numbness and avoidance) and D (increased alertness) of PTSD (all P<0.05). Different types of attachment patterns had different effects on PTSD, and the individuals with fear attachment patterns were more likely to develop PTSD. Conclusion The individuals losing their only child with insecure attachment patterns, especially the fear attachment patterns, are more likely to develop PTSD; and the prevalence rate of females losing their only child is significantly higher than that of males.

Abstract:Objective To explore the clinical efficacy of atorvastatin calcium combined with single-port double-tube irrigation and drainage in the treatment of chronic subdural hematoma. Methods The clinical prognosis and follow-up results of 42 patients with chronic subdural hematoma, who received treatment of atorvastatin calcium combined with single-hole double-tube irrigation and drainage in Department of Neurosurgery of our hospital from May 2016 to Apr. 2017, were retrospectively analyzed. There were 33 males and 9 females, aged 38-82 years with an average age of (70.0±5.8) years. Forty-two patients had headache and dizziness, and 36 patients had different severities of limb dysfunction. Results Of the 42 patients, 36 had unilateral chronic subdural hematoma and 6 had bilateral chronic subdural hematoma. The headache, dizziness and limb dysfunction were improved in all patients within 24 h after operation, and the symptoms completely disappeared after 1 month. Forty-one cases were completely cured, and cranial computed tomography (CT) examination showed that the hematoma completely disappeared at 3 months after operation in 22 cases and at 6 months after operation in 19 cases. One case (2.38%) with bilateral chronic subdural hematoma took warfarin orally due to atrial fibrillation. At 1 month after operation, the cranial CT examination showed a recurrence of chronic subdural hematoma, and the patient died of sudden cardiopulmonary arrest during follow-up. Conclusion Atorvastatin calcium combined with single-port double-tube irrigation and drainage is effective in the treatment of chronic subdural hematoma, and is worthy of popularization and application.

Abstract:Objective To explore the safety and feasibility of applying self-made peritoneal reflexed suspension device in retroperitoneoscopic partial nephrectomy for ventral renal tumors. Methods From Jan. 2018 to Jun. 2018, 15 patients with ventral renal tumors were treated by retroperitoneoscopic partial nephrectomy in Department of Urology of Eastern Hepatobiliary Surgery Hospital, Navy Medical University (Second Military Medical University). The self-made simple peritoneal retrorse suspension device was applied during the operation, and it consisted of the Hem-o-lok clamp and the associated puncture device, fixation device and clipper. Fifteen patients, aged (62.5±9.2) years, included 9 males and 6 females. All cases had single tumors located on the ventral side of the kidney, with an average maximum diameter of (2.9±1.0) cm. The clinical data, including intraoperative blood loss, operation time, renal warm ischemia time, average postoperative hospital stay and so on, were statistically analyzed. Results Retroperitoneoscopic partial nephrectomy was successfully performed on the 15 patients. The average operation time of 15 patients was (88.9±24.5) min, average renal warm ischemia time was (21.4±4.6) min, average intraoperative blood loss was (72.0±16.6) mL, and average hospital stay was (6.9±1.2) d. None of the 15 patients had blood transfusion or major surgical complications during or after operation. Conclusion This self-made peritoneal retrorse suspension device is safe and feasible in retroperitoneoscopic partial nephrectomy for ventral renal tumors, and is easy to operate during the operation. Meanwhile, it has the advantages of economy and convenience and is worthy of being popularized in clinical practice.

Abstract:Objective To explore the association between type 2 diabetes mellitus (T2DM) and hepatitis B virus-related hepatocellular carcinoma (HCC). Methods Research data were derived from a follow-up database of hepatitis B virus infection established by the Affiliated Xinyu Hospital of Nanchang University in 2010. The patients without baseline HCC were grouped into diabetic group or non-diabetic group according to whether or not they had T2DM. Cox proportional hazard regression model was used to analyze the association between T2DM and hepatitis B virus-related HCC. Results Of the 435 patients who were followed up for 2 245.89 years, 68 were new cases of HCC. The incidence density of HCC was 30.28 per thousand person-years. Sixty-four patients with T2DM were assigned to the diabetic group, and the other 371 patients were assigned to non-diabetic group. The follow-up time was 331.27 years and 1 914.62 years in the diabetic and non-diabetic groups, respectively, and the incidence densities of HCC were 51.32 per thousand person-years and 26.64 per thousand person-years, respectively; and the difference of the incidence densities was significant (χ²=6.80, P<0.05). After adjusting for age, career, smoking and drinking, Cox regression analysis showed that the risk of HCC in the T2DM patients with hepatitis B virus infection was 2.13 times that in the non-diabetic patients (95% confidence interval[CI] 1.18-3.87). Conclusion In this cohort, T2DM is associated with hepatitis B virus-related HCC, and T2DM increases the risk of HCC in hepatitis B virus infected patients.

Abstract: