Abstract:Portal vein tumor thrombus (PVTT) is one of the most important clinical characteristics of advanced hepatocellular carcinoma (HCC). The incidence of PVTT has been reported to be 44%-66.2% in patients with HCC who were firstly diagnosed, and 80%-90% were patients with advanced HCC. Once PVTT occurs in patients with HCC, the prognosis is very poor, and the natural survival time is only 2.7-4 months. The current treatment strategy for HCC patients with PVTT remains controversial. Sorafenib is recommended as the only treatment option in Europe and America; and a variety of treatment methods, including surgical treatment, transcatheter arterial chemoembolization, radiotherapy, and systemic chemotherapy, were advocated in Asia. At present, there is no consensus on diagnosis and treatment of HCC with PVTT. Our team has established a multidisciplinary team approach for diagnosis and treatment of HCC with PVTT, and has carried out systematic research and application. This article reviews the molecular mechanism of PVTT in patients with HCC, clinical diagnosis and treatment of PVTT, and the establishment and application of a multidisciplinary team approach.

Abstract:Objective To investigate the mutation types of polycystic kidney disease 1 gene (PKD1) and polycystic kidney disease 2 gene (PKD2) in Chinese patients with autosomal dominant polycystic kidney disease (ADPKD).Methods The mutations of PKD1 and PKD2 in 129 inherited ADPKD families were analyzed by long PCR and highthroughput sequencing. The positive mutation was verified by Sanger sequencing method.Results A total of 118 mutation sites of PKD1 or PKD2 in 116 inherited ADPKD families were detected from 129 families, with the detection rate being 89.9% (116/129). The mutation rates of PKD1 and PKD2 were 92.2% (107/116) and 8.6% (10/116), respectively. Of the 118 mutation sites, 80 (67.8%) were new mutations and 38 (32.2%) were known mutations; and 109 mutation sites were located in PKD1 (33 known mutations and 76 new mutations) and 9 in PKD2 (5 known mutations and 4 new mutations).Conclusion The newly discovered PKD1 and PKD2 mutations may contribute to early diagnosis and prognosis prediction of ADPKD patients, and may provide basic genetic information for clinical intervention.

Abstract:Objective To explore the relationship between exon mutations of NOTCH3 gene and clinical phenotype in patients with cerebral autosomal dominant arteriopathy with subcortical infarcts and leukoencephalopathy (CADASIL).Methods We consecutively included 30 CADASIL patients with clinical symptoms in 15 pedigrees, who visited Shanghai Ninth People's Hospital, Shanghai Jiao Tong University School of Medicine from May 2015 to Dec. 2017, and collected the clinical data and genetic analysis results. Furthermore, we analyzed the relationship between the exon mutations of NOTCH3 gene and clinical phenotypes, including age at onset, first clinical symptoms and frequency of symptomatic ischemic stroke.Results Twelve mutation sites of NOTCH3 gene were detected in the 15 pedigrees. Seven of them were located in exon 4, 3 in exon 11, 1 in exon 19, and 1 in exon 20. The onset age of the patients carrying exon 11 mutations was the latest ([53.6±13.3] years, n=7), followed by the patients carrying exon 4 mutations ([42.7±5.7] years, n=15). The onset age of 8 patients with mutations in other exons (exon 19 and 20) was (33.5±7.5) years, which was significantly earlier compared with the patients with exon 4 and 11 mutation (P<0.01 and P<0.05). Most of the patients with mutations of exon 4 had motor and speech disorders (11/15, 73.3%), while ones with mutations of exon 19 and 20 had cognitive impairment (7/8, 87.5%). Most of the patients (11/15, 73.3%) carrying mutations in exon 4 had motor and speech disorders at onset, while 7 of 8 patients (87.5%) with mutations in exon 19 and exon 20 had impaired cognition at onset. The times of symptomatic ischemic stroke in patients with mutations in exon 4 was 3 (median) and in patients with mutations in exon 11 was 2 (median), and no symptomatic ischemic stroke occurred in the patients with mutations in exon 19 and 20.Conclusion Exon 4 and exon 11 of NOTCH3 gene are hotspots of mutations in the cohort of CADASIL cases, and the mutations in different exons are associated with onset age, first symptoms and symptomatic ischemic stroke.

Abstract:Objective To explore the cell viability in the ablation area of thyroid nodules at 6 months after microwave ablation by enzyme histochemical staining.Methods Twenty-four ablation areas of thyroid nodules were selected from 20 patients who underwent histopathological assessment of the ablation area by core needle biopsy at 6 months after microwave ablation between Dec. 2017 and Sep. 2018. Core needle biopsy was performed at the central and marginal regions of the ablation area with a cutting biopsy needle. The specimens were obtained and placed in liquid nitrogen to make frozen sections. Enzyme histochemical staining was used to detect the activities of succinate dehydrogenase (SDH) and nicotinamide adenine dinucleotide phosphate diaphorase (NADPH-d), and the difference of cell morphology and histological structure was compared with H-E staining results.Results The specimens of the central and marginal regions of 24 ablation areas were successfully obtained. The histochemical staining of SDH and NADPH-d in the central region of ablation area had good consistency, and the negative rates were both 95.83% (23/24). The histochemical staining of SDH and NADPH-d in the marginal region of ablation area also had good consistency, and the negative rates were both 91.67% (22/24). H-E staining of 23 central regions and 22 marginal regions showed pink amorphous mass of necrosis. H-E staining of 1 central region and 2 marginal regions showed partly necrotic and fibrous tissue hyperplasia. The location of fibrous tissue hyperplasia was consistent with the location of the positive region of enzyme histochemical staining.Conclusion At 6 months after microwave ablation, the tissue in the ablation area of thyroid nodules is consistent with coagulative necrosis, and is still inactivated. SDH or NADPH-d enzyme histochemical staining combined with H-E staining can objectively evaluate the old ablation area.

Abstract:Objective To explore the effects of lupulone (LUP) and humulone (HUM) in Humulus lupulus L. on osteoblasts and osteoclasts of rats.Methods Osteoblasts and osteoclasts isolated from 24-h-old Wistar rats were studied and divided into control group, LUP-treated low (10^-15 mol/L)-, medium (10^-14 mol/L)- and high (10^-13 mol/L)-dose groups, and HUM-treated low (10^-15 mol/L)-, medium (10^-14 mol/L)- and high (10^-13 mol/L)-dose groups. After drug treatment, the proliferation, differentiation and bone mineralization of osteoblasts were determined by MTT assay, alkaline phosphatase (ALP) activity assay and alizarin red staining, respectively. Osteoclasts were counted and tartrate-resistant acid phosphatase (TRAP) activity was measured to evaluate the effects of LUP and HUM on the activity of osteoclasts. Osteocalcin (OCN) levels were measured by kit assay, and the expression levels of bone formation related proteins osteopontin (OPN), bone sialoprotein (BSP), bone morphogenetic protein 2 (BMP-2), bone resorption related proteins cathepsin K (CK) and matrix metalloproteinase 9 (MMP-9) were measured by Western blotting analysis to evaluate the effects of LUP and HUM on bone metabolism.Results At the osteoblast level, LUP at dosages of 10^-15 and 10^-14 mol/L could significantly promote the cell proliferation (P<0.05). LUP at dosages of 10^-14 and 10^-13 mol/L could significantly improve ALP activity and bone mineralization (P<0.05, P<0.01). LUP at dosage of 10^-13 mol/L could significantly induce the expression of OCN (P<0.01). Furthermore, LUP at dosages of 10^-14 and 10^-13 mol/L could significantly increase the expression of BSP and BMP-2 (P<0.05). HUM at dosages of 10^-15-10^-13 mol/L could also significantly promote the osteoblastic proliferation, ALP activity and bone mineralization (P<0.01), and could significantly increase the expression of OCN and OPN (P<0.05, P<0.01). Additionally, HUM at dosages of 10^-14 and 10^-13 mol/L could significantly increase the expression of BSP and BMP-2 (P<0.05). At the osteoclast level, both LUP and HUM at dosages of 10^-15-10^-13 mol/L could significantly reduce the number of osteoclasts (P<0.01) and could significantly inhibit the expression of CK (P<0.05, P<0.01). HUM at dosages of 10^-15-10^-13 mol/L could also significantly inhibit the expression of MMP-9 (P<0.05, P<0.01).Conclusion This study preliminarily clarifies that LUP and HUM can prevent bone loss by promoting bone formation and inhibiting bone resorption, which provides a new reference for the development of osteoporosis drugs.

Abstract:Objective To explore the effect of methylprednisolone (MP) with different durations on pulmonary fibrosis in rats with acute lung injury induced by smoke inhalation.Methods A total of 178 male SD rats were randomized into 5 groups:control group (n=18), simple smoke inhalation group (smoke group, n=40), smoke inhalation+ MP treatment for 1 d, 3 d and 7 d groups, naming smoke+MP (1D) group (n=40), smoke+MP (3D) group (n=40) and smoke+MP (7D) group (n=40), respectively. The rats were exposed to smoke for 30 min in the smoke box to meet the criteria of acute lung injury. MP (4 mg/kg per day) was injected intraperitoneally at 1 d, 1-3 d and 1-7 d after smoke inhalation. Survival rates were calculated at 28 d after smoke inhalation. At 7 d, 14 d and 28 d after smoke inhalation, the lung tissues were stained with Masson's trichrome staining and Sirius Red staining to evaluate pulmonary fibrosis. The mRNA expression levels of transforming growth factor β1 (TGF-β1) and α-smooth muscle actin (α-SMA) were detected by real-time fluorescent quantitative PCR, and the expression levels of myeloperoxidase (MPO), connective tissue growth factor (CTGF), high mobility group box 1 (HMGB1) and interleukin 6 (IL-6) were detected by Western blotting.Results The survival rate of the rats in the smoke group was 47.50%, and the survival rates were significantly improved to more than 80% after MP treatment with different durations (all P<0.01). Masson's trichrome staining and Sirius Red staining staining showed that the lung fibrosis of the rats in the smoke group was aggravated, and the fibrosis and collagen deposition in the smoke+MP (3D) and smoke+ MP (7D) groups were significantly attenuated compared with the smoke and smoke+MP (1D) groups (P<0.05 or P<0.01). As time progressed, the mRNA expression levels of TGF-β1 and α-SMA after smoke inhalation were increased in the smoke and smoke+MP (1D) groups. At 28 d after smoke inhalation, the TGF-β1 and α-SMA mRNA levels were significantly higher in the smoke and smoke+MP (1D) groups compared with the smoke+MP (3D) and smoke+MP (7D) groups (P<0.05 or P<0.01). At 28 d after smoke inhalation, the protein expression levels of MPO, CTGF, HMGB1 and IL-6 were significantly reduced in smoke+MP (3D) and smoke+MP (7D) groups compared with the smoke and smoke+MP (1D) groups (P<0.05, P<0.01). However, the above indicators have no significant difference between smoke+MP (3D) group and smoke+MP (7D) group (all P >0.05).Conclusion MP can significantly improve survival rate of rats with smoke inhalation injury. MP treatment for 3 d or 7 d can significantly attenuate smoke inhalation induced pulmonary fibrosis at 28 d after smoke inhalation, but there is no significant difference between these two treatment protocols.

Abstract:Objective To explore the role of curcumin in inducing autophagy and apoptosis of human hepatocarcinoma cell line Huh7, and the effect of autophagy inhibition on curcumin-induced apoptosis.Methods Human hepatocarcinoma cell line Huh7 was cultured using curcumin (5, 10, 20, and 40 μmol/L)-contained medium, and the proliferation ability was detected by CCK-8 kit. After culturing Huh7 cells using 5-40 μmol/L curcumincontained medium for 48 h, the expression levels of microtubule-associated protein 1 light chain 3 (LC3)-Ⅱ and LC3-Ⅰ were measured by Western blotting, and the ratio of LC3-Ⅱ to LC3-Ⅰ was calculated. The autophagosome was observed under fluorescence microscope. The apoptosis level of Huh7 cells was measured by flow cytometry. Then Huh7 cells were cultured using the medium containing 5 mmol/L autophagy inhibitor 3-methyladenine (3-MA) and 20 μmol/L curcumin, and the apoptotic and autophagic levels were detected.Results CCK-8 assay showed that curcumin could significantly inhibit the proliferation of Huh7 cells in a dose dependent manner (P<0.05, P<0.01). Western blotting analysis showed that curcumin significantly increased the ratio of LC3-Ⅱ to LC3-Ⅰ (P<0.05, P<0.01). Immunofluorescence microscopy showed that the number of autophagosome increased after adding 20 μmol/L curcumin. Compared with the Huh7 cells cultured with the medium containing curcumin alone at 20 μmol/L, the the ratio of LC3-Ⅱ to LC3-Ⅰ was significantly decreased in the Huh7 cells cultured with the medium containing curcumin and 3-MA (P<0.01), and the number of autophagosome decreased. Flow cytometry showed that the 5-40 μmol/L curcumin significantly induced the apoptosis of Huh7 cells (P<0.05, P<0.01), and 3-MA combined with curcumin could significantly decrease the apoptosis of Huh7 cells compared with 20 μmol/L curcumin alone (P<0.05).Conclusion Curcumin induces the apoptosis of Huh7 cells, inhibits proliferation and increases autophagy level, and inhibition of autophagy can attenuate the apoptotic effect of curcumin on Huh7 cells.

Abstract:Objective To explore the mechanism of gastrointestinal dysfunction caused by chronic renal failure (CRF), and to determine whether colon is involved in the activation of oxidative stress (OS) in CRF.Methods Thirty male SD rats were randomly divided into control group (n=10) and CRF group (n=20). The rats in the CRF group were treated with 5/6 nephrectomy to establish CRF model, and the rats in the control group were only sutured after opening renal capsule. The rats were sacrificed at 10 weeks after model administration, and the serums and colon tissues near ileocecal valve were collected. Blood urea nitrogen (BUN) and serum creatinine (SCr) were measured to evaluate the success of the model. Malonodialdehyde (MDA), superoxide dismutase (SOD), total antioxidant capacity (TAC) and 8-hydroxy deoxyguanosine (8-OHdG) in the serum and colon tissues were detected to evaluate the level of OS. The ubiquinol cytochrome C reductase core proteinⅠ(UQCRC1) was tested for the evaluation of mitochondrial function.Results Compared with the control group, the levels of BUN and SCr in serum of the rats in the CRF group were increased, suggesting that the model was successfully established. Compared with the control group, serum and colonic MDA levels were significantly increased in the CRF group (P<0.05); however, there were no significant differences in 8-OHdG or anti-oxidative markers (SOD, TAC) in serum or colon tissues between the two groups (P >0.05). The protein level of UQCRC1 in colon tissues was significantly reduced in the CRF group compared with the control group (P<0.05). However, there was no significant difference in the mRNA level of UQCRC1 in colon tissues between the control and CRF groups (P >0.05).Conclusion There is an imbalance between oxidation and antioxidation in the colonic tissues of CRF rats, which may be related to mitochondrial dysfunction.

Abstract:Objective To explore the effect of PRKAG2 gene G100S mutation in cystathionine β-synthase (CBS) region on adenosine monophosphate-activated protein kinase (AMPK) activity in cardiomyocytes of mice.Methods A human PRKAG2 (G100S) transgenic mouse model was established. Four-week-old and 12-week-old transgenic mice, and 4-week-old and 12-weekold wildtype littermate were randomly selected from N4 generation mice (n=6). The activity of AMPK in mouse cardiomyocytes was detected by phosphorylation assay kit. The difference of AMPK activity was compared between transgenic mice and wildtype littermate, and the changes of the activity of AMPK with the increase of age were observed in transgenic mice.Results The AMPK activities in cardiomyocytes of 4-week-old and 12-week-old transgenic mice were significantly lower than those of the wildtype littermate (0.042±0.013 vs 0.063±0.013, and 0.032±0.008 vs 0.062±0.018), and the differences were significant (P=0.019, P=0.004). There was no significant difference in the AMPK activity of cardiomyocytes between 4-week-old and 12-weekold transgenic mice (P=0.135).Conclusion The PRKAG2 gene G100S mutation can cause a reduction of AMPK activity in cardiomyocytes of transgenic mice, and AMPK activity does not significantly increase or decrease with the growth of the transgenic mice.

Abstract:Objective To explore the infection status of hepatitis B virus (HBV) in families of hepatitis B surface antigen (HbsAg) positive students and the mutations of HBV related to hepatocellular carcinoma, so as to provide theoretic evidence for the prevention and control of HBV infection and hepatocellular carcinoma.Methods A total of 1 611 students were investigated; they were from 60 classes of 15 schools and kindergartens in Pudong New Area, Shanghai, China, and 8 HBsAg positive students were found. These 8 students and their 18 first-degree relatives were enrolled in this study. Venous blood samples were collected to test the 5 markers of hepatitis B using enzyme linked immunosorbent assay. HBV DNA was detected by fluorescent PCR. HBV genome, basic core promoter (BCP) region and PreS region were detected using multiplex-PCR and nested PCR combined with cloning and sequencing.Results The positive rates of HBsAg and HBcAb in the firstdegree relatives were 33.3% (6/18) and 38.9% (7/18), respectively. Both HBsAg and HBcAb positive rates in the mothers were 71.4% (5/7), which were significantly higher than those of the other first-degree relatives (P<0.05). Seven of 8 families (87.5%) had 2 or more members infected or had ever infected with HBV. Fourteen of 26 members in 8 families had positive HBsAg, with a positive rate of 53.8%. A total of 4 groups of mothers and children received gene detection. Three groups of them had type C HBV gene, and 1 group had type C in mother and type B in child. Among the hepatocellular carcinomarelated HBV mutations, the mutation frequency of hot spots in BCP region was lower in the children than that in the mothers. Eight HBV mutation sites of type C in PreS region were found in both the mothers and children, and none of the remaining key sites were found in the children.Conclusion There is obvious family clustering of HBV infection, suggesting that HBV infection of students is more likely to be transmitted through mother-to-child transmission, but there are other ways of infection, such as acquired blood. The evolution degree of HBV gene in children is lower than that in mothers, which conforms to the rule of HBV evolution.

Abstract:Objective To explore the predictive value of preoperative aspartate aminotransferase-to-platelet ratio index (APRI) for post-hepatectomy liver failure (PHLF) after hepatectomy in the patients with primary liver cancer (PLC).Methods A retrospective study was conducted on the data from the PLC patients who underwent first hepatectomy in Tumor Hospital Affiliated to Guangxi Medical University between Sep. 2013 and Dec. 2016. The logistic regression model and receiver operating characteristic (ROC) curve were performed to determine the predicting values of APRI, Child-Pugh score, model for end-stage liver disease (MELD) score and albumin-bilirubin (ALBI) score for PHLF.Results A total of 1 108 PLC patients were included in this study, and PHLF occurred in 217 (19.58%) patients. The logistic regression analysis showed that Child-Pugh score, MELD score, ALBI score and APRI were predicting factors for PHLF (all P<0.05). The ROC curve analysis showed that preoperative APRI (area under curve[AUC]:0.745, 95% confidence interval[CI] 0.709-0.781, P<0.001) was significantly better for predicting PHLF compared with Child-Pugh score (AUC 0.561, 95% CI 0.516-0.605, P=0.005), MELD score (AUC 0.650, 95% CI 0.610-0.691, P<0.001) and ALBI score (AUC 0.662, 95% CI 0.621-0.703, P<0.001). Based on Youden index, the best cut-off value of preoperative APRI was 0.55 for predicting PHLF in PLC patients, with a sensitivity of 71.9% and a specificity of 68.5%, and the patients with APRI>0.55 had significantly higher overall incidence of PHLF, and higher incidence of PHLF A, B and C compared with ones with APRI ≤ 0.55 (all P<0.05).Conclusion Preoperative APRI is more accurate for predicting PHLF after hepatectomy in PLC patients versus the Child-Pugh, MELD and ALBI scores, providing guiding significance for clinical treatment of PLC.

Abstract:Obesity is a risk factor for type 2 diabetes, cardiovascular diseases, and various cancers. The prevention and treatment of obesity have become the focus of public health. Omega-3 polyunsaturated fatty acids (n-3 PUFA) are polyunsaturated fatty acids extracted from deep-sea fish. The anti-inflammatory and triglyceride-lowering properties of n-3 PUFA are well known, but its role in the treatment of obesity is still controversial. In animal models, n-3 PUFA can effectively reduce fat. However, in humans, existing studies have shown that n-3 PUFA may not contribute to weight loss, but may slow down weight gain. N-3 PUFA can improve the metabolic changes associated with obesity by regulating intestinal flora, regulating lipid metabolism, suppressing appetite, alleviating adipose tissue inflammation and altering epigenetic mechanisms, thus playing an important role in the prevention and treatment of obesity and the related metabolic diseases.

Abstract:During pregnancy, physiological state and hormone level of maternal endocrine system have great changes, providing a suitable environment for fetal growth and development. These physiologic changes have a significant impact on anesthetic physiology, pharmacology and the management techniques. With the progression of pregnancy, the changes of endocrine organs, including pituitary, thyroid, pancreas and adrenal gland are more obvious, and may cause correlated emergencies. Obstetric operations are mostly emergency operations. Any endocrine emergency during pregnancy may be life-threatening for both mothers and fetuses. This review summarizes the common endocrine emergencies during pregnancy and the key steps of diagnosis and treatment, aiming to help anesthesiologists fully understand the changes of maternal physiology, the treatment methods of the emergencies, and the key points of anesthesia management.

Abstract:Mental fatigue, first known as brain fag, is one of the common clinical fatigue types. Due to the increase of the intensity and proportion of mental work, mental fatigue has an increasing prevalence among the people working in all fields especially, especially in the military field. In recent years, mental fatigue has become an important part of fatiguerelative research. How to prevent, control and alleviate mental fatigue is an important issue, which is based on evaluating or determining the degree of mental fatigue. Because mental fatigue has strong subjectivity and its mechanism is still unclear, there are many evaluation methods, but with no unified standard. This article reviews the evaluation methods of mental fatigue, including symptomatic, psychobehavioral, and physiological and biochemical assessment, and discusses the main contents and principles of various evaluation methods, so as to provide reference to mental fatigue research.

Abstract:When providing medical services in infectious disease high-incidence areas such as Africa becomes a routine, the prevention and control of infectious diseases has become an indispensable part of hospital ship mission."Mission Harmony-2017" provided a 155-day medical service in seven African countries (Djibouti, Sierra Leone, Gabon, Congo[Brazzaville], Angola, Mozambique and Tanzania). We successfully completed the mission through active preparation before the departure, including carefully studying the prevalent trend of infectious diseases in these countries, disease screening and compulsory vaccination and drug prevention, and critical prevention and control during the mission (staff training, health and epidemic prevention and graded precaution). At last we achieved "zero" incidence of infectious diseases among mission personnel during the task. The prevention and control strategies for infectious diseases in this mission can provide experience for all kinds of personnel in areas with high-incidence of infectious diseases in the future.

Abstract:Virtual simulation technology is an effective means to construct "real" environment and experimental conditions. We investigate the characteristics and operation strategies of polar special medical virtual simulation experiment based on "data restoration-scene simulation-platform simulation-experiment research" for special medical workers for special injury treatment under polar special environment by analyzing its technical advantages and application characteristics and by applying it to polar special medicine. This may provide new research ideas and technical methods for further exploring medical service under polar special environment.

Abstract:Objective To investigate the research situation and tendency on scar caused by burns, so as to provide references for the future research.Methods The papers concerning scar caused by burns were retrieved from Web of Science core collection database. The year, country or region, affiliation, author, research direction, citation, publication journals and the impact factor of publications were analyzed using bibliometrics.Results A total of 2 247 publications related with scar caused by burns were obtained in Web of Science core collection database from 2007 to 2017, and the number of literatures had grown slowly annually. The top 1 productive country or region was America (739, 32.9%), followed by Chinese Mainland (195, 8.7%); the top 1 productive affiliation was the University of Queensland in Australia (57), and the top 1 productive author was Middelkoop E from VU University Amsterdam (50). The scientific research direction mainly focused on surgery and dermology, and the literatures mainly came from Burns and J Burn Care Res. The numbers of papers released by top 10 journals accounted 39.2% of the total numbers of related publications, and the average impact factor of top 10 journals was 2.068 9 in 2017.Conclusion The number of literatures grow slowly annually from 2007 to 2017, and most of them are published in journals with low impact factor. America, the Netherlands and Australia have done more research on scar caused by burns, which plays a significant role in promoting scientific development and academic interaction. During the past 11 years, the number of papers from Chinese Mainland has increased sharply, but the quality is still behind those of western countries.

Abstract:Objective To understand the status of social capital and quality of life in elderly patients living with human immunodeficiency virus (HIV)/acquired immunodeficiency syndrome (AIDS) in Nanchang, and to explore the correlation of social capital and its dimensions with quality of life.Methods A total of 65 elderly HIV/AIDS patients were investigated from the Center for Disease Control and Prevention (CDC) database of the Donghu District and Qingyunpu District of Nanchang from Jan. to Jun. 2015. The questionnaires included basic situation survey, personal social capital scale and simplified Chinese version of medical outcomes study HIV health survey (MOS-HIV). The descriptive statistical method was used to evaluate the status of social capital and quality of life of elderly HIV/AIDS patients. The multiple linear regression analysis was used to analyze the relationship between social capital and quality of life.Results A total of 63 questionnaires were collected. The scores of bonding social capital, bridging social capital and total social capital of elderly HIV/AIDS patients were 10.83±1.99, 7.60±2.94 and 18.43±3.47, respectively. The physical health summary score and mental health summary score were 44.25±5.10 and 42.93±5.08, respectively. Bonding social capital, CD4⁺ T cell count, age, education level and marital status were the influencing factors of quality of life (P<0.05).Conclusion The elderly HIV/AIDS patients in Nanchang have low social capital and poor quality of life. More care and help from family members, friends, neighborhoods and others are keys to improve quality of life of elderly HIV/AIDS patients, but the relationship between bridging social capital and quality of life needs to be further explored.

Abstract:Objective To establish a convenient,reliable and sensitive liquid chromatography-tandem mass spectrometry (LC-MS/MS) method for the determination of two kinds of endogenous hormones (testosterone,cortisol) in the saliva of healthy human.Methods A positive ion mode was performed on the Agilent 6410A mass spectrometer. Carbamazepine was used as the internal standard.The saliva samples were centrifuged at 21 912.8×g for 10 min with OMGEA NANOSEP 10K ultrafiltration tubes,and chromatographic separation was achieved on an Agilent ZORBAX SB-C₁₈ column with isocratic elution.The mobile phase was acetonitrile:0.1% formic acid awter solution (60:40);flow rate was 0.3 mL/min;injection volume was 10 μL;column temperature was 25℃;and the analysis period of each sample was 3 min.Results Testosterone and cortisol had good linear relationship with correlation coefficient (r) being both >0.990.Inter-and intra-day precision was both less than 15%.Conclusion This method can be used to determine the contents of testosterone and cortisol in saliva,which provides a reference for rapid and simple detection of hormone levels in vivo.

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