Abstract:Military deployment is an important factor for mental health of deployed military service members. Military deployment operations have increased greatly in recent years, so it is urgent to carry out psychological research. We reviewed status quo of post-traumatic stress disorder and other mental disorders that military deployment members suffered. Furtherly, we sorted out and summarized common psychological health interventions such as psychological screening, psychotherapy and training, and social support in military deployment. In addition, we also discussed the research directions of future military deployment including improving research methods, identifying individual differential targets for post-traumatic stress disorder, localization and militarization of related treatment technologies, and developing psychological training methods for military deployment personnel. We hope this paper can give the policymakers, researchers and those involved in military deployment information for decision-making, psychological assistant work and subsequent psychological research.

Abstract:Objective To explore the possible mechanism of human adipose-derived stem cells (hADSCs) promoting seawater immersion wound healing in vitro. Methods Human epidermal cell line HaCaT cells and artificially simulated seawater were used to establish an in vitro model of cell damage induced by seawater immersion. hADSCs were isolated from human adipose tissues, and a co-culture system of HaCaT cells and hADSCs was established. The proliferation and migration abilities of HaCaT cells were detected by cell counting kit-8 (CCK-8), 5-ethynyl-2'-deoxyuridine (EdU) cell proliferation detection kit and cell scratch test. The activation levels of epidermal growth factor receptor (EGFR)/extracellular-regulated protein kinase (ERK) signaling pathway were detected by Western blotting and real-time quantitative PCR. Results The proliferation of HaCaT cells cultured with the medium containing 10% artificial seawater was significantly inhibited compared with the cells cultured without artificial seawater (P<0.05). The proliferation and migration abilities of seawater-cultured HaCaT cells were significantly lower than those cultured without seawater and those with hADSCs and seawater (all P<0.05), but there were no significant differences in cell proliferation or migration abilities between the HaCaT cells cultured without seawater and those co-cultured with hADSCs and seawater (P>0.05). The expression of EGFR/ERK signaling pathway in seawater-cultured HaCaT cells was significantly inhibited compared with the cells cultured without seawater and those co-cultured with hADSCs and seawater (P<0.05), while the expression of EGFR/ERK signaling pathway was not significantly different between the HaCaT cells cultured without seawater and those co-cultured with hADSCs and seawater (P>0.05). Conclusion Seawater can block the activation of EGFR/ERK signaling pathway and inhibit the proliferation and migration of HaCaT cells. hADSCs can promote the activation of EGFR/ERK signaling pathway and reduce the inhibition effect of seawater against proliferation and migration of HaCaT cells.

Abstract:Objective To screen and optimize the modeling condition for radiation-induced heart damage (RIHD) models characterized by inflammatory-fibrosis pathological injury. Methods The rats were irradiated with single whole-body X-ray to screen the maximal tolerated dose. Based on the screened whole-body dose, single local heart irradiation doses were used to screen the minimal X-ray dose which could induce the significant cardiac damage. And the RIHD rat model was established by exposure to the screened dose of X-ray. Tissue samples were harvested 1 day, 1 week, 2 weeks, 4 weeks and 6 weeks after irradiation. The cardiac pathological injury score, collagen volume fraction (CVF) in myocardial tissues by Masson staining, plasma myocardial enzyme level, and the expression of inflammatory and fibrosis factors in myocardial tissues were examined for evaluating the animal model. Results The tolerance dose of whole-body irradiation was lower than 16 Gy for rats. Local irradiation dose at least 25 Gy could induce RIHD in rats. The pathological injury score of myocardial tissues, CVF in myocardial tissues and creatine kinase isoenzyme MB (CK-MB) and cardiac troponin (cTn) in plasma were increased in the RIHD model rats. Inflammatory factors including nuclear factor (NF)-κB p65, NF-κB p50 and tumor necrosis factor α (TNF-α) in myocardial tissues were increased 1 day after irradiation in the RIHD rats and maintained high to the fourth week. The expression levels of fibrotic molecules transforming growth factor β1 (TGF-β1), collagen type Ⅰ (ColⅠ) and ColⅢ in myocardial tissues were increased gradually, and reached the peaks at week 4 after irradiation. Conclusion Stable RIHD rat model can be established by irradiating the precardiac region with 25 Gy X-ray. Pathological observation and CVF can dynamically reflect the early inflammatory changes and the progression of fibrosis in RIHD rats. The sustained high expression of NF-κB p65, NF-κB p50 and TNF-α at early stage and the progressive increases of TGF-β1, ColⅠand ColⅢ can be used to evaluate the acute inflammatory injury and delayed fibrosis in the RIHD inflammatory-fibrosis model.

Abstract:Objective To detect the expression of receptor for activated C kinase 1 (RACK1), hypoxia-inducible factor 1α (HIF-1α) and vascular endothelial growth factor (VEGF) in cervical cancer, and to explore their pathological significance. Methods Eighty-five cervical cancer samples and their corresponding paracancerous tissues, which were surgically removed and confirmed by pathology in our hospital from Jun. 2014 to Jun. 2018, were included in this study. The protein expression levels of RACK1, HIF-1α and VEGF were detected by immunohistochemical staining. The relationship between the protein expression of RACK1, HIF-1α and VEGF in cervical cancer tissues and clinicopathological features, including age, tumor diameter, invasion depth, clinical stage and lymph node metastasis, were analyzed, and the correlation between the expression of RACK1, HIF-1α and VEGF was analyzed. Results Compared with paracancerous tissues, the protein expression levels of RACK1, HIF-1α and VEGF in cervical cancer tissues were significantly higher (all P<0.05), with the positive rates being 81.2% (69/85), 63.5% (54/85), and 89.4% (76/85), respectively. The expression of RACK1 was associated with clinical stage, invasion depth and lymph node metastasis (all P<0.05). The expression levels of HIF-1α and VEGF were associated with the tumor diameter, invasion depth, clinical stage and lymph node metastasis (all P<0.05). The expression levels of RACK1, HIF-1, VEGF proteins were positively correlated with each other (RACK1 vs HIF-1α, r=0.523, P=0.043 9; RACK1 vs VEGF, r=0.428, P=0.033 7; HIF-1α vs VEGF, r=0.689, P=0.024 5). Conclusion RACK1, HIF-1α and VEGF are highly expressed in cervical cancer and their expression levels are positively correlated, suggesting that they may play a synergistic role in the development and progression of cervical cancer, and can be an important marker for predicting the invasion, metastasis and prognosis.

Abstract:Objective To explore the effect of prolonged low-flow oxygen inhalation time through nasal cannula on systemic inflammatory response, intrapulmonary shunt and prognosis after hepatectomy under general anesthesia in hepatocarcinoma patients with hepatopulmonary syndrome. Methods Seventy-two patients of hepatocarcinoma with hepatopulmonary syndrome, who underwent hepatectomy in Anesthesia Department of Southwest Hospital of Army Medical University (Third Military Medical University) from Jan. 2017 to Dec. 2018, were enrolled in this study. Their American Society of Anesthesiologists (ASA) grades were classified as grade Ⅱ or Ⅲ. All patients were randomized into control group (n=36) and research group (n=36). The patients in the control group inhaled low-flow oxygen (2-3 L/min) through nasal cannula for 8 h after operation, and those in the research group for 48 h. Before anesthesia, and immediately, 8 h, 24 h, 48 h and 72 h after operation, the radial artery blood gas analysis was conducted to record arterial partial pressure of oxygen (PaO₂) and alveolar-arterial oxygen pressure difference ([A-a]DO₂). At each time point, tumor necrosis factor α (TNF-α) and lipopolysaccharide (LPS) in peripheral blood and fractional exhaled nitric oxide (FeNO) were measured as well. C-reactive protein (CRP), white blood cell count and neutrophil proportion in peripheral blood were measured 48 h after operation. The incidence of postoperative pulmonary complications and hospital stay were compared between the two groups. Results In the two groups, the PaO₂ values were significantly higher immediately and 8 h after operation versus before anesthesia, and the (A-a)DO₂ values were significantly lower (all P<0.05). At 24 h and 48 h after operation, the PaO₂ values in the research group were significantly higher than that before anesthesia, and the (A-a)DO₂ values were significantly lower than that before anesthesia (all P<0.05); while those in the control group showed the opposites (all P<0.05); and the PaO₂ values in the research group were significantly higher than those in the control group, and (A-a)DO₂ values were significantly lower (all P<0.05). The levels of LPS, TNF-α and FeNO in the research group 8, 24 and 48 h after operation were significantly lower than those before anesthesia (all P<0.05), while those in the control group 24 and 48 h after operation were significantly higher than those before anesthesia (all P<0.05). The levels of LPS, TNF-α and FeNO in the research group were significantly lower than those in the control group 24 and 48 h after operation (all P<0.05). At 48 h after operation, CRP level, white blood cell count and neutrophil proportion in the research group were significantly lower than those in the control group (all P<0.05). The incidence of postoperative pulmonary complications in the research group (1/36) was lower than that in the control group (6/36), and the hospital stay ([5.2±2.3] d) was shorter than that in the control group ([7.8±3.2] d), and the differences were statistically significant (both P<0.05). Conclusion Prolonged oxygen inhalation time (48 h) can effectively alleviate systemic inflammatory response, reduce intrapulmonary shunt and the incidence of pulmonary complications, thus facilitating postoperative recovery after hepatectomy in patients of hepatocarcinoma with hepatopulmonary syndrome.

Abstract:Objective To compare the efficacy and metabolic effects of pioglitazone-metformin and basic insulin therapy on type 2 diabetes mellitus (T2DM) patients with overweight or obesity and poor blood glucose control. Methods A total of 153 T2DM patients with overweight or obesity and poor blood glucose control were enrolled in this study. They received treatment with pioglitazone-metformin (pioglitazone-metformin group, n=77) or insulin glargine (basal insulin group, n=76) for 6 months in addition to their previous oral hypoglycemic drugs. At baseline, 3 months and 6 months after treatment, glycosylated hemoglobin (HbA_1c), fasting blood glucose (FBG), 2 h post-prandial blood glucose (2hBG), fasting insulin (FINS), 2 h post-prandial insulin (2hINS), fasting C peptide (FCp), 2 h post-prandial C peptide (2hCp), body mass index (BMI), total cholesterol (TC), triglyceride (TG), high density lipoprotein-cholesterol (HDL-C), low density lipoprotein-cholesterol (LDL-C), and hepatic fatty degeneration (expressed as controlled attenuation parameter[CAP] value) were observed and recorded. Results At baseline, there were no significant differences in gender, age, BMI, HbA_1c, FBG, 2hBG, FINS, 2hINS, FCp, 2hCp, TC, TG, HDL-C, LDL-C, CAP value, underlying diseases, or concomitant medicine between the two groups (all P>0.05). At 3 and 6 months after treatment, the HbA_1c, FBG and 2hBG levels were significantly decreased versus those at the baseline in the two groups (all P<0.01), but there were no significant differences between the two groups (all P>0.05). Compared with the basal insulin group, the FINS, BMI and CAP values were significantly decreased in the pioglitazone-metformin group 3 and 6 months after treatment (P<0.05, P<0.01). After 6 months of treatment, there were no significant changes of blood lipid levels in both groups. Conclusion In T2DM patients with overweight or obesity and poor blood glucose control, adding pioglitazone-metformin and basal insulin to their previous oral hypoglycemic drugs has similar hypoglycemic effect. However, patients receiving pioglitazone-metformin have better metabolic benefits such as lower BMI, lower insulin and improved hepatic fatty degeneration.

Abstract:Objective To evaluate the safety of modern rigid bronchoscopy related therapeutic procedures. Methods We retrospectively analyzed the clinical data of 100 patients with benign or malignant lesions who underwent modern rigid bronchoscopy operation between Apr. 2014 to Apr. 2016 at Respiratory Endoscopy Center of Changhai Hospital, Naval Medical University (Second Military Medical University). A total of 124 rigid bronchoscopy operations were performed. The intraoperative complications of rigid bronchoscopy were analyzed. Results The intraoperative complications of rigid bronchoscopy were as follows:transient hypoxemia (4.03%, 5/124), injury of vocal cords and around mucosae (4.84%, 6/124), exposure keratitis (1.61%, 2/124), airway structural failure (2.42%, 3/124), damage of electronic bronchoscope (2.42%, 3/124), and insertion failure of rigid bronchoscopy (0.81%, 1/124). There were no deaths during operation. Conclusion Modern rigid bronchoscopy is a treatment technique with high safety and few complications, and is worthy of clinical promotion and application. Standardized rigid bronchoscopy operation and related technique training should be carried out to improve its safety and reduce complications.

Abstract:Objective To explore the relationship between neutrophil-lymphocyte ratio (NLR) and the short-term prognosis of elderly patients with acute coronary syndrome (ACS). Methods The clinical data of elderly ACS patients, who were hospitalized at Department of Cardiology of Zhongda Hospital Southeast University from Jan. 2015 to Oct. 2017, were retrospectively analyzed. All patients were diagnosed by coronary angiography in combination with clinical symptoms, myocardial necrosis markers and electrocardiogram. According to NLR detected immediately after admission (within 4 h), 500 elderly ACS patients were divided into 3 groups:NLR ≤ 3.337 group (n=169), NLR 3.338-6.166 group (n=167), and NLR ≥ 6.167 group (n=164). The primary endpoints of the study were all-cause deaths during hospitalization and follow-up. The secondary outcomes were major adverse cardio-cerebrovascular events (MACCEs), hospital stay and left ventricular ejection fraction (LVEF). Results In NLR ≤ 3.337 group, NLR 3.338-6.166 group and NLR ≥ 6.167 group, the hospital stays were 8 (6, 11) d, 9 (7, 11) d and 10 (8, 11) d, the incidence rates of LVEF<50% during hospitalization were 8.9% (15/169), 14.4% (24/167) and 18.3% (30/164), and the LVEF values were (57.78±12.15)%, (54.71±11.73)% and (53.56±13.38)%, respectively, and the differences among three groups were significant (all P<0.05). The incidence of MACCEs was 21.6% (108/500) during hospitalization. Six patients died during a follow-up period of 6 months after discharge. There were no significant differences in all-cause mortality or MACCE incidence among three groups (both P>0.05). Multivariate Cox risk regression model showed that there was no association between NLR and all-cause death, MACCEs, cardiogenic death, myocardial infarction, or stroke (all P>0.05). Compared with the NLR ≤ 3.337 group, the incidence rates of LVEF<50% during hospitalization were significantly increased in the NLR 3.338-6.166 group (hazard ratio[HR]=2.567, 95% confidence interval[CI] 1.558-4.229, P<0.001) and the NLR ≥ 6.167 group (HR=1.979, 95% CI 1.629-3.524, P=0.019). Reciever operating characteristic curve showed that area under curve of NLR in evaluating LVEF<50% during hospitalization was 0.652 (95% CI 0.603-0.700, P<0.001). The optimal cut-off value of NLR was 3.84, and the sensitivity and specificity were 68.3% and 65.3%, respectively. The multiple linear regression model showed that high NLR was an independent influencing factor of prolonged hospital stay (β=0.181, P<0.001). Conclusion In elderly ACS patients, NLR is a risk factor of LVEF<50% during hospitalization and prolonged hospital stay, while it has no significant association with all-cause death and MACCEs.

Abstract:Objective To investigate the plasma microRNA-126 (miRNA-126) level in psoriasis patients before and after acitretin, methotrexate and ultraviolet phototherapy-combined treatment, and to assess its value in predicting the treatment outcomes. Methods A total of 196 patients with moderate-to-severe psoriasis were consecutively enrolled and received combined treatment with acitretin, methotrexate and ultraviolet phototherapy, and 200 volunteers were recruited as healthy controls. Plasma samples of psoriasis patients were collected and miRNA-126 level was detected at baseline and after treatment for 1 month, 3 months and 6 months. Psoriasis area and severity index (PASI) were used to assess the disease severity. Treatment response was determined by PASI 50 response (PASI decreased by 50% compared with the baseline) and PASI 75 response (PASI decreased by 75% compared with the baseline). Spearman correlation test was used to analyze the correlation between the expression level of plasma miRNA-126 and PASI. Receiver operating characteristic (ROC) curve was used to evaluate the diagnostic value of relative expression of miRNA-126 in psoriasis patients. Univariate and multivariate logistic regression models were used to analyze the influencing factors of PASI 50 and PASI 75 responses. Results Baseline miRNA-126 expression was significantly lower in psoriasis patients compared with healthy controls and was negatively correlated with PASI score (r=-0.222,P=0.002). ROC curve displayed that miRNA-126 had a good diagnostic value for psoriasis (area under curve:0.700). After treatment for 1 month, 3 months and 6 months, miRNA-126 levels were significantly elevated compared with the baseline (P<0.01). The response rates of PASI 50 were 7.1% (14/196), 37.2% (73/196) and 64.8% (127/196) after treatment for 1 month, 3 months and 6 months, and those of PASI 75 were 1.5% (3/196), 14.3% (28/196) and 35.7% (70/196), respectively. PASI 50 and PASI 75 responders presented lower baseline miRNA-126 level (P<0.05). Logistic regression analysis revealed that baseline miRNA-126 level was negatively associated with PASI 50 and PASI 75 responses. Conclusion Plasma miRNA-126 level gradually increases in psoriasis patients after acitretin, methotrexate and ultraviolet phototherapy-combined treatment, and the baseline miRNA-126 level is negatively correlated with treatment response.

Abstract:Objective To explore the mechanism of Kuhuang injection in protecting the liver and alleviating jaundice by establishing the active components-target network and protein interaction network and analyzing the functions and pathways. Methods The main active components of Kuhuang injection were obtained through traditional Chinese medicine systems pharmacology database and analysis platform (TCMSP) and related literatures. The targets to treat hepatitis and jaundice of the active components of Kuhuang injection were predicted and screened by GeneCard and online Mendelian inheritance in man (OMIM). Cytoscape 3.6.1 software was used to construct the active component-target network, and STRING database and Cytoscape 3.6.1 software were used to construct the protein interaction network. Gene ontology (GO) and Kyoto encyclopedia of genes and genomes (KEGG) pathways involved in the targets were analyzed using the database for annotation, visualization and integrated discovery (DAVID). Results The results showed that 16 active components of Kuhuang injection were screened and 85 targets were obtained. The results of network analysis showed that cellular processes, metabolic processes, and processes in response to stress were mainly involved in the effects of Kuhuang injection against hepatitis and jaundice. The results of target pathway analysis showed that the targets of Kuhuang injection for hepatitis and jaundice were mainly involved in mitogen-activated protein kinase (MAPK), Toll-like receptor, p53, neurotrophin and other signaling pathways. Conclusion The anti-hepatitis and jaundice effects of Kuhuang injection are characterized by multiple components, multiple targets and multiple pathways, which might be through regulating MAPK, Toll-like receptor, p53, neurotrophin and other related pathways.

Abstract:Cervical cancer is the second most commonly seen cancer in women and the third leading cause of cancer death in developing countries. Early diagnosis and treatment are the keys to the effective treatment of cervical cancer and the improvement of prognosis. Because of the complexity, the mechanism of development and progression of cervical cancer is still an urgent problem to solve. Recently, quantity of studies have shown that long non-coding RNAs (lncRNAs) play important roles in tumor development and progression. In this review, we sumed up the multiple effects of lncRNAs in cervical cancer, focusing on the related mechanisms of human papilloma virus (HPV) E6 and E7 oncoprotein, the nature of lncRNAs and the signaling pathway in the development and progression of cervical cancer. And we further expounded the cervical cancer mechanism network, providing reference for screening the potential markers for early diagnosis and treatment of cervical cancer.

Abstract:Post-stroke cognitive impairment (PSCI) is an important cause of post-stroke disability, and it is also one of focuses of stroke research. The incidence of PSCI is high, which affects the recovery of neurologic function and lowers the quality of life of patients. Therefore, neurologists should be alert to PSCI, and guide patients to actively prevent PSCI to improve the prognosis. In this paper, we reviews the research status of the incidence, influencing factors, neuropsychological assessment, imaging research and prevention measures of PSCI.

Abstract:Objective To explore the feasibility and clinical effects of Double-Pulley technique combined with 8-shaped tension band in teatment of comminuted fractures of distal patellar pole. Methods From Jan. 2017 to Apr. 2018, 10 patients (6 males and 4 females) were treated with Double-Pulley technique combined with 8-shaped tension band in our hospital. The ages of patients ranged from 28 to 58 years old, with an average of (36.4±11.4) years old. The average time from injury to surgery was (2.8±1.1) d, ranging from 1 to 5 d. The injury cause was traffic accident in 6 cases, falling iniury in 3 cases, and sport injury in 1 case. Bostman's patellar fracture function evaluation system was used to evaluate the postoperative knee joint function. The clinical evaluation indexes included the excellent and good rate of Bostman's patellar fracture function score, and X-ray examination results and knee mobility during follow-up. Results All patients were followed up for 16 to 24 months. The incision of all patients healed well, and no anterior knee pain or complications were found. Six months after surgery, X-ray examination showed that the patellar fracture healing of 10 patients were all osteonal union. Six months after surgery, the flexion range of knee joint was 132.0°±12.3°, and Bostman's patellar fracture function score was 28.5±1.8, with the excellent and good rate being 100% (excellent in 9 cases and good in 1 case). Conclusion Double-Pulley technique combined with 8-shaped tension band is effective and reliable in the treatment of comminuted fractures of distal patellar pole, and the patients can start functional exercise early after surgery.

Abstract:Under the information condition, there remain many problems in consciousness, training talents, training contents and training methods in the combat readiness training of mobile medical service forces in military hospitals, which reduces the pertinence and effectiveness of combat readiness training and also affects the effect of combat readiness training. In this review, we systematically analyzed the bottleneck problems of combat readiness training in the above 4 aspects, providing new ideas for effectively solving them, so as to truly implement the combat readiness training and effectively improve the service of military hospital mobile medical service forces.

Abstract:New concept weaponry have much greater damage effects than traditional weaponry, not only destroying military equipment and communication systems, but also severely injurying combatants. Typical new concept weaponry, including shipborne laser weapons, electromagnetic pulse weapons and infrasonic weapons, holds destructive power by special light, sound, or electromagnetic wave. This paper expounds the injury effects of new concept weapons on combatants and its medical protection measures, so as to provide reference for the health service support under the condition of new concept weapons.

Abstract:Objective To investigate the therapeutic effect of double filtration plasmapheresis (DFPP) on severe anti-N-methyl-D-aspartate receptor (NMDAR) encephalitis and its clinical significance. Methods We retrospectively analyzed the clinical data from 9 patients with severe anti-NMDAR encephalitis confirmed by Changhai Hospital of Naval Medical University (Second Military Medical University) from Jan. 2014 to Mar. 2018. The 9 patients did not respond to methylprednisolone shock therapy. We collected the clinical manifestations, and examination results of laboratory, electroencephalogram and imaging, and analyzed the therapeutic effect of DFPP. Results Nine anti-NMDAR encephalitis patients, including 5 females and 4 males, were admitted to neurology intensive care unit. Their ages were ranged from 15 to 69 years old, median age of onset was 37 years old, and average hospital stay was (33.2±7.6) d. The main clinical symptoms were mental behavioral abnormalities (9 cases), autonomic dysfunction (9 cases), seizures (7 cases), central hypopnea (5 cases), and consciousness disorders (5 cases). One patient was complicated with ovarian teratoma. Nine patients were positive for anti-NMDAR antibodies in cerebrospinal fluid, and 7 patients were positive for anti-NMDAR antibodies in serum. All the 9 patients were examined by electroencephalogram, and 7 of them had abnormal findings, mainly with diffuse changes and abnormal slow waves. Brain magnetic resonance imaging showed that abnormal signals could be seen in the frontal lobe, parietal lobe, temporal lobe, hippocampus and other brain regions of 4 patients, and no abnormal signals were found in the other 5 patients. Nine patients were treated with DFPP after ineffective treatment with methylprednisolone, 5 of them recovered completely, and the other 4 cases had significantly improved residual symptoms. Conclusion DFPP can be used as an alternative for patients with severe anti-NMDAR encephalitis who are not sensitive to glucocorticoid therapy. It has better clinical efficacy and it is not restricted by allogeneic plasma resources.

Abstract:Objective To explore the microsurgical treatment of spinal canal tumors and to analyze the strategies for clinical treatment of spinal canal tumors. Methods Were retrospectively analyzed the clinical data of 145 patients with spinal canal tumors who were treated in our hospital from Dec. 2014 to Nov. 2016. Microscopic resection of tumors was performed with half lamina-, full lamina-, or lamina joint-fenestration. Spinal internal fixation was performed in 52 patients. The patients were followed up for 3-26 months. X-ray and magnetic resonance imaging were performed again in all patients, and computed tomographyed was performed in patients undergoing internal fixation. The spinal cord function was assessed by McCormick grade before operation, 1 week after operation and 3 months after operation. Results Of the 145 patients, 127 underwent total resection, 16 underwent subtotal resection and 2 underwent partial resection. One patient with cervical intramedullary tumors developed kyphosis after operation and underwent reoperation. No screw rod dislocation or spine instability was found in the 52 patients undergoing spinal internal fixation. The McCormick grading was significantly better 1 week and 3 months after operation versus before operation (P<0.05). Conclusion Microsurgery is an effective method for treatment of spinal canal tumors. Good intraoperative exposure and reliable spinal internal fixation are essential to reduce the complications after spinal canal tumor surgery.

Abstract:Objective To understand the anxiety and depression status of students before finishing the standardized resident training program (SRTP) and the influencing factors, so as to provide evidence-based basis for improving the mental health and management system for those residents. Methods A questionnaire survey was conducted by combining the self-rating anxiety scale (SAS) and the self-rating depression scale (SDS). The participants were the graduating students of SRTP from two affiliated hospitals of a military medical university, and they were surveyed and analyzed in Jul. 2018. Results A total of 252 questionnaires were distributed and 252 valid questionnaires were returned, with a recovery rate of 100%. There were 123 (48.8%) males and 129 (51.2%) females, with an average age of (28.3±2.1) years old. There were 87 (34.5%) undergraduate students, 138 (54.8%) master degree student, and 27 (10.7%) doctor degree students. Twenty-seven (10.7%) students were trained for one year, 96 (38.1%) for 2 years, and 129 (51.2%) for 3 years. There were 151 (59.9%) students from "985" universities or "211" universities (key universities in China), and 101 (40.1%) from other universities. Twenty (7.9%) participants were in military service. We found that 94.4% (238/252) of the graduating students were in an anxious state and 60.3% (152/252) in a depress state. The severity of anxiety was related to the length of training (χ²=14.214, P=0.027). Depression was related to the type of university ("985" or "211" universities vs others, χ²=5.691, P=0.017; military colleges vs regional colleges, χ²=5.069, P=0.024). No relation was found between depression and anxiety state in the participants (r=0.025, P=0.696). Conclusion Graduating students from SRTP are faced with multiple pressures such as medical job, employment and graduation, and the incidence rates of anxiety and depression are much higher than those of the general population and medical staff. Sufficient attention and intervention measures should be actively taken for prevention of depression and axiety.

Abstract:Objective To explore the pharmacokinetics of self-made gliclazide modified release tablets in Beagle dogs and to evaluate the in vivo and in vitro correlation. Methods Six Beagle dogs were orally given self-made gliclazide modified release tablets or reference preparation (DaMeiKang) at a dose of 30 mg with self-control cross-over method. Blood samples were collected at different time points after administration. The gliclazide concentration in plasma was determined by high-performance liquid chromatography, and the pharmacokinetic parameters were calculated. The pharmacokinetic characteristics and relative bioavailability of self-made gliclazide modified release tablets were investigated, the bioequivalence was evaluated, and the in vivo and in vitro correlation was calculated. Results Area under curve (AUC_0-∞) of DaMeiKang was (101.74±20.29) μg/(mL·h), and AUC_0-∞ of self-made gliclazide modified release tablets was (95.40±28.68) μg/(mL·h). There were no significant differences in the pharmacokinetic parameters between the test and reference formulations (P>0.05). The relative bioavailability of self-made gliclazide modified release tablets was 93.77%, which was bioequivalent with the reference preparation. The in vitro and in vivo correlation analysis showed that the correlation coefficients of DaMeiKang and self-made gliclazide modified release tablets were 0.912 and 0.894, respectively, which were higher than the critical value (r_{0.05, 7}=0.754). The in vitro release rates of the two preparations were correlated with the in vivo absorption rates. Conclusion The self-made gliclazide modified release tablets have sustained-release characteristics and bioequivalence with reference preparation. The in vivo absorption behavior of gliclazide modified release tablets can be predicted by the in vitro release assay established in this study.

Abstract:Endometriosis is a common clinical disorder in premenopausal women. Although endometriosis is a benign gynecologic disorder, malignant ovarian tumors have been documented to arise in women with endometriosis. The pathogenesis by which endometriosis associated ovarian cancer develops remain poorly understood. Here we report a case of EAOC, and discuss the potential pathogenesis, clinicopathologic features and clinical regime.