Abstract:Training a large number of qualified general practitioners and promoting the hierarchical medical system are key measures to deepen the reform of the medical and health system and implement the “Healthy China Strategy”. After more than 30 years of development, China’s general practice has made significant progress in the cultivation of a talent team, the establishment of a training system, the exploration of service models, and the cultivation of scientific research. However, China’s general practice started late and has a poor foundation. At present, it still faces challenges: quantity and quality of talents need to be improved, the teaching staff and training mode need to be optimized, the hierarchical medical system is not fully implemented, the application channels of research projects are not smooth, and the career attraction is low. Multiple measures should be taken to improve the quantity and quality of general practitioners, to guide the public opinion on seeking medical treatment, to set up more specific funds of general practice to expand the scientific research of general practice, and to reform the incentive and promotion mechanism to enhance the career attraction, so as to promote the sustainable development of general practice.

Abstract:Objective To study the dynamic changes of astrocytes in brain tissue after ischemic stroke (IS) by single cell sequencing technology, so as to better understand the role of astrocytes in the development and progression of IS. Methods The sequencing data of IS mouse brain tissue (GSE227651) were downloaded from the Gene Expression Omnibus (GEO) database, and the canonical correlation analysis (CCA) method was used for data integration. Different cell subsets were obtained by t-distributed stochastic neighbor embedding (tSNE) dimensionality reduction and cluster analysis, and different cell subsets were annotated by SingleR package. Further, different astrocyte subsets were obtained by tSNE dimensionality reduction and cluster analysis, and the number change and functional status of different astrocyte subsets were analyzed. Monocle package was used to analyze the developmental stages of different astrocyte subsets. Dynamic analysis of ligand-receptor interactions between astrocytes and other cell subsets was performed using CellChat package. Results The sequencing data of GSE227651 were downloaded and the mouse brain cells were grouped into 19 cell subsets and annotated into 16 different cell types after integrated cluster analysis. The astrocytes were further divided into 6 astrocyte subsets by cluster analysis. Compared with the healthy control, the number of subsets 0 and 3 decreased on the 1^st day after IS, but gradually increased on the 3^rd and 7^th day after IS; the number of subsets 2 and 5 increased on the 1^st day after IS, and gradually decreased on the 3^rd and 7^th day after IS; subsets 1 and 4 had little change at different time points after IS. Based on functional analysis, astrocyte subsets 2 and 5 were defined as reactive astrocytes, subsets 0 and 3 were defined as reparative astrocytes, and subsets 1 and 4 were defined as resting astrocytes. According to the results of pseudotime analysis, astrocyte subset 2 was further defined as acute reactive astrocytes, and subset 0 was defined as reparative astrocytes after ischemic injury. The analysis of cell ligand-receptor interactions showed that the ligand-receptor interactions between astrocytes and astrocytes, B cells, natural killer (NK) cells, endothelial cells, macrophages, epithelial cells and neurons changed dynamically with time, and the inflammatory response was mediated mainly by establishing association with NK cells on the 1^st day after IS. The recovery of neurovascular function was mediated by the connection with endothelial cells and neurons from the 3^rd day of IS. Conclusion The functional status of astrocytes changes dynamically with time after IS. In the early period after IS, acute reactive astrocytes may mediate the acute inflammatory response after injury, while from the 3^rd day after IS, reparative astrocytes may participate in the functional reconstruction of brain tissue after injury.

Abstract:Objective To analyze the expression of ferroptosis-related prognostic genes in hepatocellular carcinoma (HCC) based on single cell sequencing data, so as to provide effective targets for precise treatment of HCC. Methods The Cancer Genome Atlas (TCGA) database and ferroptosis-related gene database were used to obtain ferroptosis-related differentially expressed genes in HCC patients and normal tissue samples, and least absolute shrinkage and selection operator (LASSO) regression analysis was used to construct risk prognosis assessment model. Using single cell sequencing data, we analyzed the expression of prognostic risk genes in HCC cells at different developmental stages by t-distributed stochastic neighbor embedding (tSNE) dimensionality reduction and Monocle package, and the interactions between prognostic risk genes in HCC cells were further analyzed by CellChat package. Results A total of 25 ferroptosis-related differentially expressed genes were found to be associated with the prognosis of HCC patients. And 5'-nucleotidase domain containing 2 (NT5DC2), glucose-6-phosphate dehydrogenase (G6PD), stathmin1 (STMN1) and interleukin 33 (IL-33) were selected as HCC prognostic genes. Among them, NT5DC2 was mainly expressed in epithelial and endothelial cells, IL-33 was mainly expressed in endothelial cells, and G6PD and STMN1 were widely expressed in cells. IL-33 was mainly expressed in endothelial cells at early developmental stages, while NT5DC2, G6PD and STMN were expressed at different developmental stages. Moreover, IL-33 interacted extensively with its receptor only in endothelial cells at early stage of development. Conclusion As a benign prognostic gene, IL-33 is specifically expressed and plays a role in endothelial cells at the early stage of HCC development, suggesting that maintaining this primitive state of vascular endothelial cells or promoting IL-33-mediated primitive endothelial ferroptosis may be an effective therapeutic target for HCC.

Abstract:Cell-to-cell variation and heterogeneity are fundamental and intrinsic characteristics of stem cell populations, but these differences are masked when bulk cells are used for traditional omic analysis. Technologies such as single-cell genome, epigenome and transcriptome sequencing have been developing rapidly in recent years. Single cell sequencing technologies serve as powerful tools to comprehensively dissect cellular heterogeneity and to identify distinct phenotypic cell types. The application of these methods to different types of stem cells, including pluripotent stem cells and tissue-specific stem cells, has led to exciting new findings in the stem cell field. This paper reviews the recent progress as well as future perspectives in the methodology and application of single-cell omic sequencing technologies.

Abstract:Single-cell transcriptome sequencing refers to the technology of reverse transcription, amplification, and sequencing of all RNA from a single cell, followed by bioinformatics, and it has developed rapidly. Due to its ability to interpret cell heterogeneity at the single cell level, single-cell transcriptome sequencing has been widely used in various fields of life science, especially in the research of diseases such as tumors. This article mainly introduces the process and development of single-cell transcriptome sequencing, and analyzes its application in oncology research such as tumor heterogeneity, tumor metastasis, tumor microenvironment, and anti-tumor drug development.

Abstract:The combination of single-cell transcriptome sequencing and spatial transcriptome sequencing can analyze the transcriptome and spatial location information of each cell within the tissue, which helps to explore the gene expression level of cells and the 3-dimensional reconstruction of tissue, decipher cell heterogeneity, understand the connections and interactions between cells, and deeply explore the cellular and molecular mechanisms of disease development and progression. Fibrotic diseases involve multiple organs, the course of the disease is extremely complex, and there is a lack of effective anti-fibrotic treatment; it is still necessary to strengthen the comprehensive understanding of the disease. In recent years, single-cell transcriptome sequencing combined with spatial transcriptome sequencing has been applied for the research on pathophysiological mechanisms of fibrotic diseases, and more progress has been made. This article briefly introduces single-cell transcriptome sequencing combined with spatial transcriptome sequencing and reviews its research progress in fibrotic diseases.

Abstract:Objective To analyze the distribution characteristics and clinical significance of serum allergens in hospitalized children with asthma. Methods A total of 355 children with wheezing symptoms who were admitted to our pediatrics department from Jan. 2012 to Feb. 2021 and completed allergen detection were enrolled, and the results of allergen specific immunoglobulin E (sIgE) detection and the levels of serum total immunoglobulin E (T-IgE) were retrospectively analyzed. According to the diagnosis, the children were divided into asthma group (109 cases) and non-asthma group (246 cases), and the differences in allergen distribution and serum T-IgE levels were compared between the 2 groups. Results Among the 355 children, 40.6% (144 cases) were positive for allergens. The top 3 inhalation allergens with positive detection were dust mite (82.6%, 119/144), house dust (10.4%, 15/144) and cat dander (10.4%, 15/144). The top 3 food allergens were egg white (12.5%, 18/144), cod/lobster/scallop (10.4%, 15/144) and milk (10.4%, 15/144). Compared with the non-asthma group, the age of the asthma group was significantly older (［8.8±2.9］years vs ［2.4±1.4］years); the positive rate of allergen sIgE (71.6%［78/109］vs 26.8%［66/246］), the inhalational allergen positive rate (54.1%［59/109］ vs 16.3%［40/246］), the multiple sensitization rate (26.6%［29/109］ vs 10.2% ［25/246］) and the T-IgE level (344.0［108.0, 920.0］ IU/mL vs 79.6 IU/mL［26.2, 280.5］) of the asthma group were significantly higher (all P＜0.05). Conclusion The immunopathology of asthma in children is mainly characterized by high-IgE. There are differences in the positive rate and distribution of allergens between asthmatic children and non-asthmatic children. The positive rate of allergens is higher, the numbers of inhaled allergens and multiple sensitization are greater, and the level of T-IgE is higher in asthmatic children.

Abstract:Objective To explore the feasibility and accuracy of deep learning in automatic identification of standard views of pediatric echocardiography. Methods A total of 4 035 pediatric echocardiography images from the picture archiving and communication system database of Shanghai Children’s Medical Center, Shanghai Jiao Tong University School of Medicine, which were collected from Sep. to Oct. 2022, were selected and randomly divided into training set (2 421 images), validation set (807 images), and testing set (807 images) in a ratio of 6∶2∶2. A lightweight and efficient deep learning model was developed by improving DenseNet to achieve automatic identification of 15 standard views of pediatric echocardiography, and was compared with 3 commonly used deep learning models, including DenseNet121, InceptionV3, and MobileNetV3. With manual annotation results as the gold standard, the identification performance of deep learning models was evaluated using accuracy, precision, specificity, recall, and F1 score. The efficiency of the identification model was evaluated using 3 indicators: the number of parameters, model size, and floating-point operations. The identification results of the model were displayed using a confusion matrix, and the model’s concerns to image features were reflected using a heatmap. Results The average F1 scores of DenseNet121, InceptionV3, MobileNetV3, and the proposed model for identifying 15 standard and non-standard views of pediatric echocardiography were 94.59%, 95.13%, 92.41%, and 94.73%, the numbers of parameters were 7.0×10⁶, 24.4×10⁶, 4.2×10⁶, and 1.8×10⁶, the model sizes were 13.941, 48.777, 8.445, and 3.588 MB, and the floating-point operations were 11.16×10⁹, 12.89×10⁹, 0.86×10⁹, and 3.05×10⁹, respectively. The confusion matrix and heatmap showed that the proposed model had a higher recognition rate for 15 standard and non-standard views of pediatric echocardiography than DenseNet121, InceptionV3 and MobileNetV3, and was able to focus on the key feature areas in the ultrasonic views. Conclusion The deep learning model proposed in this study can accurately identify standard cardiac ultrasound views in children; moreover, the model has a small number of parameters and can be operated with high efficiency.

Abstract:Objective To construct and validate a prognostic model for sorafenib sensitivity-related genes in hepatocellular carcinoma (HCC) based on bioinformatics methods, and to explore the predictive ability of the model for prognosis and the efficacy for sorafenib treatment in HCC. Methods Differential gene analysis was performed on GSE109211 data set of Gene Expression Omnibus, LIHC cohort of The Cancer Genome Atlas (TCGA) and LIRI cohort of International Cancer Genome Consortium (ICGC). Sorafenib sensitivity-related genes in HCC were screened by intersection, and Kyoto Encyclopedia of Genes and Genomes (KEGG) signal pathway enrichment analysis was performed. A prognostic model was constructed using univariate Cox regression and least absolute shrinkage and selection operator (LASSO) regression, and the patients were divided into high-risk and low-risk groups based on the median risk score. Survival analysis was conducted using Kaplan-Meier method and multivariate Cox regression analysis. The half inhibitory concentration (IC₅₀) of sorafenib was analyzed through the Genomics of Drug Sensitivity in Cancer (GDSC) and its relationship with risk score was explored. Results A total of 365 sorafenib sensitivity-related genes in HCC were screened, and the KEGG enrichment analysis revealed the presence of pathways associated with drug metabolism. Univariate Cox analysis identified 221 genes associated with prognosis, and a prognostic model containing 7 key genes (chaperonin containing TCP1 subunit 3［CCT3］, erythropoietin［EPO］, formimidoyltransferase cyclodeaminase［FTCD］, glucose-6-phosphate dehydrogenase［G6PD］, kinesin family member 20A［KIF20A］, phosphatidylinositol glycan anchor biosynthesis class U［PIGU］, and secreted phosphoprotein 1 ［SPP1］) was constructed by LASSO regression. Risk score＝CCT3×0.032＋EPO×0.055＋FTCD×(－0.026）＋G6PD×0.083＋KIF20A×0.039＋PIGU×0.144＋SPP1×0.009. The results showed that the high-risk group had a shorter survival time compared to the low-risk group (P＜0.001). Multivariate Cox analysis demonstrated that risk score was an independent prognostic factor. The IC₅₀ of sorafenib in the high-risk group was lower than that in the low-risk group, suggesting that the high-risk group may be more sensitive to sorafenib. Conclusion The constructed prognostic model for HCC based on sorafenib sensitivity-related genes has good prognostic value for HCC.

Abstract:Objective To select a high-level disinfection method suitable for disposable portable bronchoscope, and to explore the application value of adenosine triphosphate (ATP) bioluminescence detection in the evaluation of disinfection effect. Methods Ten new disposable portable bronchoscopes were randomly divided into control group (n＝1) and 3 experimental groups (hypochlorous acid group ［n＝3］, peracetic acid group ［n＝3］, and low-temperature plasma group ［n＝3］). The control group did not receive any treatment. The hypochlorous acid group, peracetic acid group and low-temperature plasma group were disinfected respectively by hypochlorous acid immersion, peracetic acid immersion and low-temperature plasma sterilization after simulated clinical aspiration. The bronchoscopes in each experimental group were pretreated and decontaminated by incomplete immersion. The time of hypochlorous acid immersion, peracetic acid immersion, and low-temperature plasma sterilization was 3, 5, and 45 min, respectively. The disinfection effect was evaluated by ATP bioluminescence detection and bacterial culture. Results The results of ATP bioluminescence detection showed that the relative luminous unit (RLU) values of bronchoscopic lumen sampling solution in the hypochlorous acid group and peracetic acid group after 5 rounds of disinfection were between 16 and 179 (all values＜200), and the qualified rates of disinfection were 100% in both groups. After 2 rounds of disinfection in the low-temperature plasma group, the RLU values of bronchoscopic lumen sampling solution were between 675 and 4 532 (all values＞200), and the qualified rates of disinfection were 0. After 5 rounds of disinfection, the results of bacterial culture in the hypochlorous acid group and peracetic acid group showed that colony-forming units were between 0 and 6, and the qualified rates were 100%. After 2 rounds of disinfection, the results of bacterial culture in the low-temperature plasma group showed that colony-forming units were greater than 20, and the qualified rates were 0. There were no significant differences in the image quality, distal curvature or negative pressure attraction of the bronchoscopes between the experimental groups and the control group after each round of disinfection. Conclusion Both hypochlorous acid immersion disinfection and peracetic acid immersion disinfection can achieve a high level of disinfection without affecting the performance of bronchoscope after the pretreatment of disposable portable bronchoscope. The results of ATP bioluminescence detection are consistent with those of bacterial culture, and it can quickly evaluate the disinfection effect of disposable portable bronchoscope.

Abstract:Military training injury presents a high incidence trend in our army, and the acute and chronic pain caused by the injury has a significant impact on combat effectiveness. Transdermal analgesics have special advantages and potential application value in the treatment of military training injury pain due to its convenient use, quick effect, high controllability, and few adverse reactions. This paper reviews and prospects the application of transdermal analgesics in the treatment of military training injury pain from the perspectives of commonly used clinical analgesics, such as opioids, nonsteroidal anti-inflammatory drugs, local anesthetics, and transient receptor potential vanilloid type 1 channel activators, so as to better guide the clinical treatment.

Abstract:With the development of information technology and the improvement of ultrasonic instrument performance, portable and intelligent ultrasonic equipment has played an important role in many clinical fields. Remote technology has made the ultrasonic diagnosis and treatment free from the restriction of location, and the applied field has been expanded. In this review, we summarized the application status of portable remote ultrasound in clinical disease diagnosis, emergency rescue, perioperative assistance, military medical support, and student teaching, which fully reflected its unique advantage of “small size and multiple functions”. Based on the characteristics of high speed and low delay of 5G technology, its expanded application was also prospected.

Abstract:Objective To explore the relationship between sleep, coping tendency, anxiety and subjective well-being among crew of surface warship, and to elucidate the psychological mechanism of sleep affecting subjective well-being. Methods A total of 295 crew members of surface warship were selected by random sampling method. Athens insomnia scale, generalized anxiety disorder-7, simplified coping style questionnaire and Memorial University of Newfoundland scale of happiness were used for questionnaire survey. Pearson correlation analysis and mediation effect test were used for data analyses. Results A total of 288 valid questionnaires were collected, with an effective rate of 97.63%. The results of the correlation analysis showed that sleep was negatively correlated with coping tendency and subjective well-being, coping tendency was negatively correlated with anxiety, and anxiety was negatively correlated with subjective well-being (all P＜0.01); sleep was positively correlated with anxiety, and coping tendency was positively correlated with subjective well-being (both P＜0.01). The analysis of chain mediating effect showed that the sleep of the members could affect the subjective well-being not only through the single partial mediating effects of coping tendency and anxiety, but also through the partial chain mediating effects of coping tendency and anxiety. Conclusion Coping tendency and anxiety play a part of chain mediating role between sleep and subjective well-being among crew members of surface warship.

Abstract:Objective To investigate the relationship between sleep status and traditional Chinese medicine (TCM) constitution of people stationed on an island. Methods Cluster sampling method was adopted to investigate the personnel stationed on the island using the self-designed demographic questionnaire, Pittsburgh sleep quality index (PSQI) scale and TCM constitution scale, and 722 qualified questionnaires were obtained. Spearman rank correlation was used to analyze the relationship between sleep status and accumulative time on island. Pearson chi-square test was used to compare the distribution of TCM constitution between insomnia and non-insomnia people. Binary stepwise logistic regression analysis was used to screen the main influencing factors of TCM constitution for insomnia. Results Among the 722 people surveyed, 308 (42.7%) had no sleep disorder and 414 (57.3%) suffered from insomnia. There was a significant difference in the distribution of TCM constitution between insomnia and non-insomnia people (all P＜0.01). The balanced constitution was less in the insomnia personnel, and the other 8 kinds of biased constitution were more. The 3 types of constitution with higher proportion were qi-stagnation constitution (89.6%, 138/154), blood-stasis constitution (88.7%, 149/168), and phlegm-dampness constitution (86.0%, 160/186). The results of binary stepwise logistic regression analysis showed that the balanced constitution (β＝－0.647, OR＝0.524, P＝0.036) was a protective factor for insomnia among personnel stationed on island, while qi-deficiency constitution (β＝0.654, OR＝1.906, P＝0.032), dampness-heat constitution (β＝0.607, OR＝1.834, P＝0.042), and qi-stagnation quality (β＝0.705, OR＝2.025, P＝0.049) were risk factors for insomnia. Conclusion The sleep quality of personnel stationed on island is related to the type of TCM constitution. Balanced constitution is a protective factor of insomnia, while qi-deficiency, dampness-heat, and qi-stagnation constitution are the risk factors of insomnia.

Abstract:Objective To explore the diagnostic value of deep learning model based on calcification characteristic parameters for breast ductal carcinoma in situ (DCIS). Methods From Jan. 2016 to Dec. 2022, data of patients with calcification found by mammography in Shenzhen Hospital of Southern Medical University or Cancer Center of Sun Yat-sen University were retrospectively analyzed. According to the pathological diagnosis results, the patients were divided into benign group (569 cases) and DCIS group (263 cases). The calcification feature detection and classification model were established by deep learning, and the characteristic parameters with statistically significant differences were screened. The receiver operating characteristic curve (ROC) was used to analyze the diagnostic effectiveness of each characteristic parameter, the deep learning model and traditional machine learning models on DCIS. Results Among the 20 characteristic parameters, 5 (the number of linear branching-like calcification, the number of granular calcification, rate of segment distribution, rate of clustered distribution, and population density) had significant differences between the 2 groups, and the DCIS group had higher values (all P＜0.05). The area under curve (AUC) values of ROC in the number of linear branching-like calcification, the number of granular calcification, rate of segment distribution, rate of clustered distribution and population density for identifying DCIS were 0.762, 0.732, 0.725, 0.757 and 0.810, respectively, with sensitivities of 81.2%, 85.9%, 80.1%, 87.8% and 86.4%, and specificities of 76.0%, 71.6%, 70.3%, 73.4% and 63.7%, respectively. The AUC value, sensitivity and specificity of the deep learning model established by the combination of the 5 feature parameters for recognition of DCIS were 0.823, 89.3% and 77.3%, respectively, which were higher than those of independent feature parameters and traditional machine learning models, including support vector machine, K-nearest neighbor, linear discriminant analysis, and logistic regression (AUC values were 0.771, 0.801, 0.765, and 0.734, sensitivities were 79.7%, 80.9%, 77.8%, and 74.4%, and specificities were 57.8%, 62.9%, 56.6%, and 47.9%, respectively). Conclusion Screening the calcification features with high diagnostic value and establishing a deep learning model are helpful to the diagnosis of DCIS with mammograms.

Abstract:Objective To observe the hemostatic effect of pre-arranged pericardial wrapping in total arch replacement for type A aortic dissection (AAD). Methods The data of 35 patients who were diagnosed as AAD and underwent total arch replacement with pre-arranged pericardial wrapping hemostasis in our hospital from Jan. to Nov. 2021 were retrospectively analyzed. Thirty-five AAD patients who underwent total arch replacement with traditional hemostatic methods in the same period were selected as controls. The intraoperative conditions (including the aortic root treatment, cardiac arrest time, cardiopulmonary bypass time, operation time, and blood transfusion during surgery), early postoperative blood coagulation function (including the platelet count, prothrombin time, fibrinogen concentration, K time, and maximum clot strength 1 d after operation), and postoperative recovery (including the drainage volume 24 h after operation, the second thoracotomy for hemostasis, mechanical ventilation time, hemofiltration, and intensive care unit［ICU］ admission time) of the pre-packaged group and the control group were observed. Results There were no significant differences in the aortic root treatment, cardiac arrest time, the second thoracotomy for hemostasis, mechanical ventilation time, hemofiltration, or ICU admission time between the 2 groups (all P＞0.05). Compared with the control group, the pre-packaged group had shorter cardiopulmonary bypass time and operation time, and less blood transfusion during the operation (all P＜0.05). Compared with the control group, the pre-packaged group had higher platelet count, fibrinogen concentration, maximum clot strength, and shorter prothrombin time and K time 1 d after operation (all P＜0.05). The drainage volume 24 h after operation in the pre-packaged group was less than that in the control group (P＜0.05). Conclusion Pre-arranged pericardial wrapping for hemostasis in AAD total arch replacement can reduce operation time and perioperative blood loss, protect the coagulation function of patients, and help patients recover after surgery, so it can be used as a clinical hemostasis method.

Abstract:Objective To evaluate the effectiveness of dorsal bridge plating combined with Kirschner wire elastic fixation in the treatment of Myerson type B Lisfranc injury. Methods The clinical data of 16 patients with Lisfranc injury who underwent surgical treatment in Department of Orthopaedics, Baoshan Luodian Hospital and Department of Orthopaedics Trauma, The First Affiliated Hospital of Naval Medical University (Second Military Medical University) from Jun. 2018 to Dec. 2020 were collected. There were 10 males and 6 females with an average age of (44.5±12.1) years (range 25-65 years). There were 9 cases of left foot injury and 7 cases of right foot injury. The time from injury to operation was (7.5±1.7) d (range 5-11 d). According to the Myerson classification, all cases were type B. The second tarsometatarsal joint was fixed with dorsal bridge plating, and the dorsal ligament was also repaired. Furthermore, the medial and lateral columns were fixed with Kirschner wire. The wound healing was observed and the complications were recorded. At the final follow-up, the effectiveness and function were assessed by American Orthopaedic Foot and Ankle Society ankle-hindfoot scale (AOFAS-AHS), and the pain condition was assessed by visual analogue scale (VAS). Results All patients were followed for up 12-20 months, with an average of (17.2±2.2) months. All the incisions healed by the first intention. No incision infection, skin necrosis, osteofascial compartment syndrome, deep peroneal nerve injury, lower extremity deep venous thrombosis, Kirschner wire migration, internal fixation failure or traumatic osteoarthritis were observed in any patients. At the last follow-up, the AOFAS-AHS score was 86.3±3.4 (range 70-95), including excellent in 10 cases, good in 4 cases, and fair in 2 cases, with an excellent and good rate of 87.5% (14/16); the VAS score was 1.7±0.9 (range 1-4). Conclusion For patients with Myerson type B Lisfranc injury, the treatment of rigid fixation of the second tarsometatarsal joint with dorsal bridge plating, repair of the dorsal ligament, and elastic fixation of the medial and lateral columns with Kirschner wire can obtain satisfactory clinical results.

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