Most cited articles
2009, 30(6):610-612.
DOI: 10.3724/SP.J.1008.2009.0610
Abstract:
Since the first reported case of swine influenza A in Mexico, a total of 15 510 cases have been confirmed in 53 countries by May 29, 2009.On April 29, 2009, the World Health Organization (WHO) raised its pandemic alert from grade 4 to grade 5.The virus is described as a new subtype of A/H1N1 and is not detected in pigs or humans previously.The virus is sensitive to oseltamivir and zanamivir, but resistant to amantadine and rimantadine.The genetics of the virus are so novel that humans are unlikely to have much immunity to it.The virus can transmit from human to human; therefore it is necessary to enforce quarantine measure for close contactors because the virus transmits during latency.Precaution methods like covering noses and mouths with a tissue when coughing or sneezing can reduce the transmission opportunity.Hands should be washed frequently with soap, especially after coughing or sneezing.Public places with ventilation conditions, personal health behavior and health condition are critical for the prevention and control of this epidemic.
Since the first reported case of swine influenza A in Mexico, a total of 15 510 cases have been confirmed in 53 countries by May 29, 2009.On April 29, 2009, the World Health Organization (WHO) raised its pandemic alert from grade 4 to grade 5.The virus is described as a new subtype of A/H1N1 and is not detected in pigs or humans previously.The virus is sensitive to oseltamivir and zanamivir, but resistant to amantadine and rimantadine.The genetics of the virus are so novel that humans are unlikely to have much immunity to it.The virus can transmit from human to human; therefore it is necessary to enforce quarantine measure for close contactors because the virus transmits during latency.Precaution methods like covering noses and mouths with a tissue when coughing or sneezing can reduce the transmission opportunity.Hands should be washed frequently with soap, especially after coughing or sneezing.Public places with ventilation conditions, personal health behavior and health condition are critical for the prevention and control of this epidemic.
2007, 28(6):0676-0677.
DOI: 10.3724/SP.J.1008.2007.00676
Abstract:
2007, 28(5):0562-0563.
DOI: 10.3724/SP.J.1008.2007.00562
Abstract:
2017, 38(4):397-404.
DOI: 10.16781/j.0258-879x.2017.04.0397
Abstract:
Osteoporosis is a frequently seen metabolic bone disease characterized by reduced bone mass, reduced bone mineral density and bone micro-structure destruction. Patients with osteoporosis are prone to brittle fractures, and the incidence of osteoporosis is increased annually. The pathogenesis of osteoporosis is related to the imbalance between osteoblasts and osteoclasts. On one hand, the activity of osteoclasts is increased, with high bone resorption; on the other hand, the function of osteoblasts is attenuated, with low osteogenesis; and finally the two reasons lead to loss of net bone mass. Osteogenesis is closely related to the differentiation of bone marrow-derived mesenchymal stem cells (BMSCs). In osteoporotic patients, the adipogenic differentiation of BMSCs is increased and the osteogenic differentiation is decreased. The differentiation fate of BMSCs is regulated by BMP/Smad, Wnt, Notch, Hedgehogs and other signal pathways, involving microRNAs, transcription factors, epigenetic and other regulatory mechanisms, which is a focus in current research. The future studies need to focus on finding the key factors in determining the differentiation fate of BMSCs and BMSCs transplantation, so as to cast new lights on the treatment of osteoporosis.
Osteoporosis is a frequently seen metabolic bone disease characterized by reduced bone mass, reduced bone mineral density and bone micro-structure destruction. Patients with osteoporosis are prone to brittle fractures, and the incidence of osteoporosis is increased annually. The pathogenesis of osteoporosis is related to the imbalance between osteoblasts and osteoclasts. On one hand, the activity of osteoclasts is increased, with high bone resorption; on the other hand, the function of osteoblasts is attenuated, with low osteogenesis; and finally the two reasons lead to loss of net bone mass. Osteogenesis is closely related to the differentiation of bone marrow-derived mesenchymal stem cells (BMSCs). In osteoporotic patients, the adipogenic differentiation of BMSCs is increased and the osteogenic differentiation is decreased. The differentiation fate of BMSCs is regulated by BMP/Smad, Wnt, Notch, Hedgehogs and other signal pathways, involving microRNAs, transcription factors, epigenetic and other regulatory mechanisms, which is a focus in current research. The future studies need to focus on finding the key factors in determining the differentiation fate of BMSCs and BMSCs transplantation, so as to cast new lights on the treatment of osteoporosis.
2008, 29(1):0083-0086.
DOI: 10.3724/SP.J.1008.2008.00083
Abstract:
Objective：To separate the nematocyst venom (NV) and tentacle-only extract (TOE) from the jellyfish Cyanea capillata and to analyze the difference between their haemolytic activities and the influencing factors.Methods: NV and TOE were separated by autolysis and centrifugation.The influence of concentration, temperature and pH value on the haemolytic activities of NV and TOE were observed.Results: NV and TOE were successfully separated.The concentration-associated haemolytic curves were “S” shaped for both NV and TOE.The HU50 of NV and TOE were 8 μg/ml and 67 μg/ml, respectively; and the haemolysis of NV was about 8.4 times of that of TOE.Temperature had great influence on haemolysis of both and the highest haemolysis were both at 40℃.pH value also had influence on haemolysis.The strongest haemolysis activity was found both at pH 8 and TOE was more sensitive.Conclusion: NV and TOE both have haemolysis activity,with the activity of NV stronger than that of the latter.The haemolysis activity is influenced by concentration, temperature and pH value.
Objective：To separate the nematocyst venom (NV) and tentacle-only extract (TOE) from the jellyfish Cyanea capillata and to analyze the difference between their haemolytic activities and the influencing factors.Methods: NV and TOE were separated by autolysis and centrifugation.The influence of concentration, temperature and pH value on the haemolytic activities of NV and TOE were observed.Results: NV and TOE were successfully separated.The concentration-associated haemolytic curves were “S” shaped for both NV and TOE.The HU50 of NV and TOE were 8 μg/ml and 67 μg/ml, respectively; and the haemolysis of NV was about 8.4 times of that of TOE.Temperature had great influence on haemolysis of both and the highest haemolysis were both at 40℃.pH value also had influence on haemolysis.The strongest haemolysis activity was found both at pH 8 and TOE was more sensitive.Conclusion: NV and TOE both have haemolysis activity,with the activity of NV stronger than that of the latter.The haemolysis activity is influenced by concentration, temperature and pH value.
2007, 28(11):1219-1222.
DOI: 10.3724/SP.J.1008.2007.01219
Abstract:
Objective：To study the expression of F1ATPaseα in nonsmall cell lung cancer and its clinical significance. Methods: (1)The expression level of F1ATPaseα was examined by immunohistochemical Envision method in 38 NSCLC samples and the adjacent normal tissues, 7 benign tumors and chronic pneumonia samples. (2)F1ATPaseα mRNA expression were detected in 12 fresh samples of NSCLC and the adjacent normal tissues by PTPCR.(3)The expression of F1ATPaseα on the pericellular membrane of A549 cells was observed by immunofluorescence. Results: (1)Immunohistochemistry analysis showed median and higher expression of F1ATPaseα in 36 of NSCLC specimens and 11 adjacent normal tissues; lower expression was detected in all the benign samples.The overexpression of F1ATPaseα in lung adenocarcinoma tissues were significantly higher than that in the lung squamous cancer (P<0.05). The expression of F1ATPaseα in NSCLC was not associated with the histology type, location, differentiation degree, size, invasion and metastasis. (2) The relative level of ECTOF1ATPaseα was 0.31±0.12 in the adjacent normal tissues and 0.54±0.19 in NSCLC tissues (P<0.01). (3)The conspicuous positive expression of F1ATPaseα on the pericellular membrane of A549 cells was not observed on normal bronchial epithelial cells.Conclusion: （1）NSCLC has a higher expression of F1ATPaseα and the expression is on the pericellular membrane of A549 cells, which may provide a new target for molecular therapy of NSCLC.
Objective：To study the expression of F1ATPaseα in nonsmall cell lung cancer and its clinical significance. Methods: (1)The expression level of F1ATPaseα was examined by immunohistochemical Envision method in 38 NSCLC samples and the adjacent normal tissues, 7 benign tumors and chronic pneumonia samples. (2)F1ATPaseα mRNA expression were detected in 12 fresh samples of NSCLC and the adjacent normal tissues by PTPCR.(3)The expression of F1ATPaseα on the pericellular membrane of A549 cells was observed by immunofluorescence. Results: (1)Immunohistochemistry analysis showed median and higher expression of F1ATPaseα in 36 of NSCLC specimens and 11 adjacent normal tissues; lower expression was detected in all the benign samples.The overexpression of F1ATPaseα in lung adenocarcinoma tissues were significantly higher than that in the lung squamous cancer (P<0.05). The expression of F1ATPaseα in NSCLC was not associated with the histology type, location, differentiation degree, size, invasion and metastasis. (2) The relative level of ECTOF1ATPaseα was 0.31±0.12 in the adjacent normal tissues and 0.54±0.19 in NSCLC tissues (P<0.01). (3)The conspicuous positive expression of F1ATPaseα on the pericellular membrane of A549 cells was not observed on normal bronchial epithelial cells.Conclusion: （1）NSCLC has a higher expression of F1ATPaseα and the expression is on the pericellular membrane of A549 cells, which may provide a new target for molecular therapy of NSCLC.
2007, 28(2):0175-0178.
DOI: 10.3724/SP.J.1008.2007.00175
Abstract:
Objective：To access the application of percutaneous coronary angioplasty via radial artery in treatment of acute myocardial infarction. Methods： Totally 107 patients with acute myocardial infarction （AMI） were included in this study. Transradial approach （group A） percutaneous coronary intervention （PCI） was used in 56 patients and the transfemoral approach （group B） PCI was used in 51 patients. The successful rates of puncture procedure and PCI, time of artery cannulation, the cannulation to balloon inflation times, the total procedure time, and local puncture complications were compared between the 2 groups. Results： The successful rates of puncture procedure were 100% in both groups and their successful rates of PCI were higher than 96%. The time of artery cannulation, the cannulation to balloon inflation time, and the total procedure time were similar in group A and group B （[2.93±0.42] min vs [3.07±0.54] min, P=0.14; [17.23±3.47] minvs [16.81±4.86] min, P=0.61; [47.04±7.53] min vs [48.74±6.22] rain, P=0.21, respectively）. The incidence of bleeding and edema around puncture sites in group B was higher than that in group A （5/51 vs 0/56, P=0. 016 4）3 the vagal reaction rate in group B was higher than that in group B （4/51 vs 0/56, P=0. 032 7）. Conclusion： Transradial access is a safe and feasible technique for performing percutaneous coronary intervention in treatment of acute myocardial infarction; it can decrease major bleeding complications
Objective：To access the application of percutaneous coronary angioplasty via radial artery in treatment of acute myocardial infarction. Methods： Totally 107 patients with acute myocardial infarction （AMI） were included in this study. Transradial approach （group A） percutaneous coronary intervention （PCI） was used in 56 patients and the transfemoral approach （group B） PCI was used in 51 patients. The successful rates of puncture procedure and PCI, time of artery cannulation, the cannulation to balloon inflation times, the total procedure time, and local puncture complications were compared between the 2 groups. Results： The successful rates of puncture procedure were 100% in both groups and their successful rates of PCI were higher than 96%. The time of artery cannulation, the cannulation to balloon inflation time, and the total procedure time were similar in group A and group B （[2.93±0.42] min vs [3.07±0.54] min, P=0.14; [17.23±3.47] minvs [16.81±4.86] min, P=0.61; [47.04±7.53] min vs [48.74±6.22] rain, P=0.21, respectively）. The incidence of bleeding and edema around puncture sites in group B was higher than that in group A （5/51 vs 0/56, P=0. 016 4）3 the vagal reaction rate in group B was higher than that in group B （4/51 vs 0/56, P=0. 032 7）. Conclusion： Transradial access is a safe and feasible technique for performing percutaneous coronary intervention in treatment of acute myocardial infarction; it can decrease major bleeding complications
2007, 28(2):0227-0228.
DOI: 10.3724/SP.J.1008.2007.00227
Abstract:
1 临床资料 患者，男．55岁。因“颈椎人工椎间盘置换术（cervical artificial disc replacement，CADR）后四肢活动障碍13个月”于2006年8月3日入院。患者于13个月前因“行走不稳、双手麻木半年”于外院行C5/6 Bryan Disc置换术，麻醉苏醒后呈四肢瘫。当地予神经营养、高压氧、针灸等治疗后，屈肘、肩关节外展自主运动部分恢复。术后侧位片C5/6间隙见人工椎间盘（artificialdisc，AD，图1A）；[第一段]
1 临床资料 患者，男．55岁。因“颈椎人工椎间盘置换术（cervical artificial disc replacement，CADR）后四肢活动障碍13个月”于2006年8月3日入院。患者于13个月前因“行走不稳、双手麻木半年”于外院行C5/6 Bryan Disc置换术，麻醉苏醒后呈四肢瘫。当地予神经营养、高压氧、针灸等治疗后，屈肘、肩关节外展自主运动部分恢复。术后侧位片C5/6间隙见人工椎间盘（artificialdisc，AD，图1A）；[第一段]
2009, 30(5):565-568.
DOI: 10.3724/SP.J.1008.2009.0565
Abstract:
Objective：To apply 1HNMR spectroscopic analysis and principal components analysis(PCA) for investigating the fluctuation of metabolites between hydrocortisone-induced kidney deficiency syndrome(Shenxu Zheng) rats and the normal rats.Methods: Using 1HNMR based metabolic profiling and PCA,we investigated the difference of urine metabolites between the normal rats and rats with hydrocortisone-induced kidney deficiency syndrome.We also explained the changes of the related metabolic pathway or metabolic network.Results: The changes following hydrocortisone intervention of rats could be identified by PCA,and the kidney deficiency syndrome rats entered a state of “hyperfunction”,involving a series of changes in metabolic pathway and metabolic network.The response integral area of lactic acid(δ 1.37) increased，indicating the accumulation of metabolites of lactic acid.The amount of dimethylamine(δ 2.72) also increased,indicating that the disorder of kidney function was related to kidney-deficiency syndrome.The response of creatinine(δ 3.87),asparaginic acid(δ 2.83),taurine(δ 3.44,3.28),and hippurate(δ 7.84,7.56,7.64,3.97) all decreased significantly,indicating that the hydrocortisone might cause adrenal cortex excretion injury.The response integral area of succinate(δ 2.41) and citric acid(δ 2.53,2.68) decreased,which was usually caused by disorder of the mitochondrial function.Conclusion: It is suggested that the metabonomic approach can be used to investigate the pathophysiological changes under certain physiopathologic conditions.This study provides evidence for understanding “Shenxu Zheng”(kidney deficiency syndrome) in traditional Chinese medicine and for diagnosis and therapy of such diseases.
Objective：To apply 1HNMR spectroscopic analysis and principal components analysis(PCA) for investigating the fluctuation of metabolites between hydrocortisone-induced kidney deficiency syndrome(Shenxu Zheng) rats and the normal rats.Methods: Using 1HNMR based metabolic profiling and PCA,we investigated the difference of urine metabolites between the normal rats and rats with hydrocortisone-induced kidney deficiency syndrome.We also explained the changes of the related metabolic pathway or metabolic network.Results: The changes following hydrocortisone intervention of rats could be identified by PCA,and the kidney deficiency syndrome rats entered a state of “hyperfunction”,involving a series of changes in metabolic pathway and metabolic network.The response integral area of lactic acid(δ 1.37) increased，indicating the accumulation of metabolites of lactic acid.The amount of dimethylamine(δ 2.72) also increased,indicating that the disorder of kidney function was related to kidney-deficiency syndrome.The response of creatinine(δ 3.87),asparaginic acid(δ 2.83),taurine(δ 3.44,3.28),and hippurate(δ 7.84,7.56,7.64,3.97) all decreased significantly,indicating that the hydrocortisone might cause adrenal cortex excretion injury.The response integral area of succinate(δ 2.41) and citric acid(δ 2.53,2.68) decreased,which was usually caused by disorder of the mitochondrial function.Conclusion: It is suggested that the metabonomic approach can be used to investigate the pathophysiological changes under certain physiopathologic conditions.This study provides evidence for understanding “Shenxu Zheng”(kidney deficiency syndrome) in traditional Chinese medicine and for diagnosis and therapy of such diseases.
2009, 30(8):937-940.
DOI: 10.3724/SP.J.1008.2009.0937
Abstract:
Objective:To study the chemical constituents of Polygonum capitatum. Methods:Isolation and purification of the chemical constituents were carried out repeatedly by silica gel column and Sephadex gel column chromatography. The structures of compounds were identified by physical-chemical methods and spectral data. Results:Fourteen compounds were identified from the petroleum ether fraction of P. capitatum:tricosanol (Ⅰ),pentacosanol (Ⅱ),docosanoic acid (Ⅲ),lignoceric acid (Ⅳ),hexadecanoic-2,3-dihydroxy-propyleste (Ⅴ),docosanoic-2,3-dihydroxypropyleste (Ⅵ),oleanolic acid (Ⅶ),ursolic acid (Ⅷ),octacosa-1,27-diene (Ⅸ),docosyl ferulate (Ⅹ),β-sitosterol (Ⅺ),tricosane (Ⅻ),hexadecanoic acid (),and linoleic acid (). Conclusion:Compounds Ⅰ-Ⅹ are obtained from P. capitatum for the first time. Compounds Ⅸ and Ⅹare obtained from Polygonum for the first time.
Objective:To study the chemical constituents of Polygonum capitatum. Methods:Isolation and purification of the chemical constituents were carried out repeatedly by silica gel column and Sephadex gel column chromatography. The structures of compounds were identified by physical-chemical methods and spectral data. Results:Fourteen compounds were identified from the petroleum ether fraction of P. capitatum:tricosanol (Ⅰ),pentacosanol (Ⅱ),docosanoic acid (Ⅲ),lignoceric acid (Ⅳ),hexadecanoic-2,3-dihydroxy-propyleste (Ⅴ),docosanoic-2,3-dihydroxypropyleste (Ⅵ),oleanolic acid (Ⅶ),ursolic acid (Ⅷ),octacosa-1,27-diene (Ⅸ),docosyl ferulate (Ⅹ),β-sitosterol (Ⅺ),tricosane (Ⅻ),hexadecanoic acid (),and linoleic acid (). Conclusion:Compounds Ⅰ-Ⅹ are obtained from P. capitatum for the first time. Compounds Ⅸ and Ⅹare obtained from Polygonum for the first time.
2009, 30(11):1292-1296.
DOI: 10.3724/SP.J.1008.2009.01292
Abstract:
Objective:To identify the components for nurse job satisfaction,and to develop a scale for job satisfaction assessment in Chinese nurses.Methods:Using literature review and expert consulting,we developed a primary scale consisting of 10 factors and 56 items.After testing with 135 nurses,a nurse job satisfaction scale composing of 8 factors and 38 items was developed,and was used to test 1 185 nurses; the reliability and efficacy of the scale were also assessed.Results:The developed nurse job satisfaction scale was composed of 8 factors (administration,work loads,relationship with colleagues,job,salary and welfare,professional opportunities,work recognition by others,and balance between family and work) and 38 items,and it had good reliability and efficacy.Conclusion:The developed scale can not only help to evaluate job satisfaction among nurses,but also provide evidence for corresponding intervention.
Objective:To identify the components for nurse job satisfaction,and to develop a scale for job satisfaction assessment in Chinese nurses.Methods:Using literature review and expert consulting,we developed a primary scale consisting of 10 factors and 56 items.After testing with 135 nurses,a nurse job satisfaction scale composing of 8 factors and 38 items was developed,and was used to test 1 185 nurses; the reliability and efficacy of the scale were also assessed.Results:The developed nurse job satisfaction scale was composed of 8 factors (administration,work loads,relationship with colleagues,job,salary and welfare,professional opportunities,work recognition by others,and balance between family and work) and 38 items,and it had good reliability and efficacy.Conclusion:The developed scale can not only help to evaluate job satisfaction among nurses,but also provide evidence for corresponding intervention.
XIE Jia-xin△
,
YIN Jian-hua△
,
LI Shu-hua
,
LU Wen-ying
,
HAN Yi-fang
,
HAN Lei
,
ZHANG Hong-wei
,
CAO Guang-wen
2009, 30(6):613-617.
DOI: 10.3724/SP.J.1008.2009.0613
Abstract:
Objective:To investigate the evolutionary relationship of the hemagglutinin（HA）gene of novel influenza virus A/H1N1 in 2009 pandemic with the HA genes of A/H1N1 viruses isolated in different parts of the world previously. Methods: The sequences of the HA gene of the novel A/H1N1 strain and the reference sequences of human, swine, and avian influenza A viruses were retrieved from NCBI. MEGA 4.0 software was employed to align, blunt nucleotide sequences, and construct phylogenetic tree. The deduced amino acid sequences of the HA genes of novel influenza virus A/H1N1 were compared with those of the A/H1N1 isolates in North America, Europe, and Asia.Results: Phylogenetic tree of the HA genes of A/H1N1 strains worldwide showed that the HA genes of novel influenza virus A/H1N1 in 2009 shared a high homology with those of the 7 human A/H1N1 influenza viruses isolated in North America during 1976 to 2007, and shared a low homology with those of the human influenza viruses A/H1N1 isolated in Europe and Asia. Phylogenetic tree of the HA gene between different species showed that the HA genes of novel influenza virus A/H1N1 in 2009 had a close evolutionary relationship with those of the two swine A/H1N1 strains isolated in 1998 and 2007 in North America,but a distant evolutionary relationship with those of swine and avian A/H1N1 isolated in Europe and Asia. Alignment of amino acid at important antigenic sites showed that the HA gene of the novel A/H1N1 strains shared important antigen sites with the swine A/H1N1 influenza viruses isolated in North America, and did not share with the swine A/H1N1 influenza viruses isolated in Europe and Asia or the human A/H1N1 influenza vaccine strains.Conclusion: The HA genes of the novel influenza virus A/H1N1 might originate from swine A/H1N1 influenza viruses in North America after a long time evolution and the reassortment with fragments of human A/H1N1 in the area, and the current A/human/H1N1 influenza vaccine may not be effective for the novel A/H1N1 virus.
Objective:To investigate the evolutionary relationship of the hemagglutinin（HA）gene of novel influenza virus A/H1N1 in 2009 pandemic with the HA genes of A/H1N1 viruses isolated in different parts of the world previously. Methods: The sequences of the HA gene of the novel A/H1N1 strain and the reference sequences of human, swine, and avian influenza A viruses were retrieved from NCBI. MEGA 4.0 software was employed to align, blunt nucleotide sequences, and construct phylogenetic tree. The deduced amino acid sequences of the HA genes of novel influenza virus A/H1N1 were compared with those of the A/H1N1 isolates in North America, Europe, and Asia.Results: Phylogenetic tree of the HA genes of A/H1N1 strains worldwide showed that the HA genes of novel influenza virus A/H1N1 in 2009 shared a high homology with those of the 7 human A/H1N1 influenza viruses isolated in North America during 1976 to 2007, and shared a low homology with those of the human influenza viruses A/H1N1 isolated in Europe and Asia. Phylogenetic tree of the HA gene between different species showed that the HA genes of novel influenza virus A/H1N1 in 2009 had a close evolutionary relationship with those of the two swine A/H1N1 strains isolated in 1998 and 2007 in North America,but a distant evolutionary relationship with those of swine and avian A/H1N1 isolated in Europe and Asia. Alignment of amino acid at important antigenic sites showed that the HA gene of the novel A/H1N1 strains shared important antigen sites with the swine A/H1N1 influenza viruses isolated in North America, and did not share with the swine A/H1N1 influenza viruses isolated in Europe and Asia or the human A/H1N1 influenza vaccine strains.Conclusion: The HA genes of the novel influenza virus A/H1N1 might originate from swine A/H1N1 influenza viruses in North America after a long time evolution and the reassortment with fragments of human A/H1N1 in the area, and the current A/human/H1N1 influenza vaccine may not be effective for the novel A/H1N1 virus.
2007, 28(3):0294-0298.
DOI: 10.3724/SP.J.1008.2007.00294
Abstract:
Objective：To investigate the chemical constituents in the roots of Angelica dahurica var. formosana. Methods： The dried rhizomes of Angelica dahurica var. formosana were chopped and extracted with 80% EtOH for 3 times under reflux. The EtOH extract was isolated by chromatography on silica gel using a gradient solvent system （PE： EtOAc ＆ CHCl3 ： MeOH） and Sephadex LH-20. The structures of the contents were elucidated by nuclear magnetic resonance and MS spectral analysis. Results： Seventeen compounds were identified, namely, isoimperatorin （ 1 ）, imperatorin （ 2 ）, bergapten （ 3 ）, alloimperatorin（ 4 ）, xanthotoxol （ 5 ）, cnidilin （ 6 ）, byakangelicin （ 7 ）, bergaptol （ 8 ）, byakangelicol （ 9 ）, pabulenol （ 10 ）, oxypeucedanin hydrate（ 11 ）, desmodimine （ 12 ）, palmitic acid （ 13 ）, stiamasterol （ 14 ）, β-sitosterol （ 15 ）, β-daucosterin （ 16 ）, and sucrose（17）. Conclusion： Compounds 8 and 12 have been isolated from Angelica dahurica var. formosana for the first time. Compound 12 has been firstly isolated from Umbelifera plants. All the courmarin compounds belong to linear 6, 7- furanocourmarins.[
Objective：To investigate the chemical constituents in the roots of Angelica dahurica var. formosana. Methods： The dried rhizomes of Angelica dahurica var. formosana were chopped and extracted with 80% EtOH for 3 times under reflux. The EtOH extract was isolated by chromatography on silica gel using a gradient solvent system （PE： EtOAc ＆ CHCl3 ： MeOH） and Sephadex LH-20. The structures of the contents were elucidated by nuclear magnetic resonance and MS spectral analysis. Results： Seventeen compounds were identified, namely, isoimperatorin （ 1 ）, imperatorin （ 2 ）, bergapten （ 3 ）, alloimperatorin（ 4 ）, xanthotoxol （ 5 ）, cnidilin （ 6 ）, byakangelicin （ 7 ）, bergaptol （ 8 ）, byakangelicol （ 9 ）, pabulenol （ 10 ）, oxypeucedanin hydrate（ 11 ）, desmodimine （ 12 ）, palmitic acid （ 13 ）, stiamasterol （ 14 ）, β-sitosterol （ 15 ）, β-daucosterin （ 16 ）, and sucrose（17）. Conclusion： Compounds 8 and 12 have been isolated from Angelica dahurica var. formosana for the first time. Compound 12 has been firstly isolated from Umbelifera plants. All the courmarin compounds belong to linear 6, 7- furanocourmarins.[
YANG Bo△
,
XIAO Liang△
,
WANG Hui-qing
,
WANG Lin-hui
,
XU Chuan-liang
,
HOU Jian-guo
,
XU Bin
,
SUN Ying-hao
2010, 31(4):417-420.
DOI: 10.3724/SP.J.1008.2010.0417
Abstract:
Objective To try to perform single-port laparoscopic nephrectomy in pigs, and to summarize and improve the basic skills, so as to decrease risks in clinical practice. Methods From Apr. to May 2009, we performed 10 times (5 pigs) of nephrectomy on porcine model using Triport system. The experience was summarized with respect to the following issues: the choice of endoscopy and instruments, insertion of Triport single port system, and optimization of the basic operation procedure. Results All the procedures were successful, without bleeding, organ injury or other complications. Operative time decreased from 75 min at the first time to 23 min for the last one. Clashing of instruments was the biggest frustration in the process; “X” shape crossing can partially resolve the problem, based on which four kinds of basic operating modes were designed. Conclusion Single-port laparoscopy nephrectomy in pigs can help the new learners to quickly improve their skills and decreases clinical risks.
Objective To try to perform single-port laparoscopic nephrectomy in pigs, and to summarize and improve the basic skills, so as to decrease risks in clinical practice. Methods From Apr. to May 2009, we performed 10 times (5 pigs) of nephrectomy on porcine model using Triport system. The experience was summarized with respect to the following issues: the choice of endoscopy and instruments, insertion of Triport single port system, and optimization of the basic operation procedure. Results All the procedures were successful, without bleeding, organ injury or other complications. Operative time decreased from 75 min at the first time to 23 min for the last one. Clashing of instruments was the biggest frustration in the process; “X” shape crossing can partially resolve the problem, based on which four kinds of basic operating modes were designed. Conclusion Single-port laparoscopy nephrectomy in pigs can help the new learners to quickly improve their skills and decreases clinical risks.
15 To enhance academic communification and to upgrade the academic level of educational institution?
2007, 28(6):0656-0658.
DOI: 10.3724/SP.J.1008.2007.00656
Abstract:
2007, 28(5):0495-0499.
DOI: 10.3724/SP.J.1008.2007.00495
Abstract:
2007, 28(7):0752-0755.
DOI: 10.3724/SP.J.1008.2007.00752
Abstract:
多年来，肥胖症的分子发病机制一直不清楚，直到1994年瘦素（leptin）ob基因和1995年瘦素受体（OB-R）基因的成功克隆，才使肥胖症分子发病机制的研究进入了新的时代。现介绍ob基因、瘦素受体及其信号转导的研究情况。[第一段]
多年来，肥胖症的分子发病机制一直不清楚，直到1994年瘦素（leptin）ob基因和1995年瘦素受体（OB-R）基因的成功克隆，才使肥胖症分子发病机制的研究进入了新的时代。现介绍ob基因、瘦素受体及其信号转导的研究情况。[第一段]
