Most view articles
2019, 40(2):223-225.
DOI: 10.16781/j.0258-879x.2019.02.0223
Abstract:
Objective To explore the effect of glucocorticoid on ocular surface changes in the patients with thyroid-associated ophthalmopathy. Methods Sixty eyes of 30 patients with thyroid-associated ophthalmopathy were examined for the break-up time of tear film (BUT), blinks, incomplete blinks, tear film lipid layer thickness (LLT) and the fluidity of tear film lipid layer before and after glucocorticoid treatment. The differences of each detection index were compared and analyzed. Results The BUT of the patients with thyroid-associated ophthalmopathy was significantly longer after glucocorticoid treatment versus before glucocorticoid treatment (the medians were 5.0 s and 7.0 s, respectively; P<0.01). The average, maximal and minimal values of LLT were significantly increased after glucocorticoid treatment than those before glucocorticoid treatment (average, maximal and minimal values of LLT before and after treatment were 59 nm and 64.5 nm, 73.5 nm and 78.8 nm, and 52.4 nm and 57.5 nm, respectively; all P<0.01). There were no significant differences in the blinks, incomplete blinks or the fluidity of tear film lipid layer between before and after glucocorticoid treatment (all P>0.05). Conclusion Glucocorticoid treatment can improve the lipid secretion of tear film, thus maintaining the stability of tear film.
Objective To explore the effect of glucocorticoid on ocular surface changes in the patients with thyroid-associated ophthalmopathy. Methods Sixty eyes of 30 patients with thyroid-associated ophthalmopathy were examined for the break-up time of tear film (BUT), blinks, incomplete blinks, tear film lipid layer thickness (LLT) and the fluidity of tear film lipid layer before and after glucocorticoid treatment. The differences of each detection index were compared and analyzed. Results The BUT of the patients with thyroid-associated ophthalmopathy was significantly longer after glucocorticoid treatment versus before glucocorticoid treatment (the medians were 5.0 s and 7.0 s, respectively; P<0.01). The average, maximal and minimal values of LLT were significantly increased after glucocorticoid treatment than those before glucocorticoid treatment (average, maximal and minimal values of LLT before and after treatment were 59 nm and 64.5 nm, 73.5 nm and 78.8 nm, and 52.4 nm and 57.5 nm, respectively; all P<0.01). There were no significant differences in the blinks, incomplete blinks or the fluidity of tear film lipid layer between before and after glucocorticoid treatment (all P>0.05). Conclusion Glucocorticoid treatment can improve the lipid secretion of tear film, thus maintaining the stability of tear film.
YANG Zhi-gao
,
ZHANG Fan
,
CHEN Zhi
,
CHENG Yong-jun
,
LI Feng-ning
,
L Kai-yang
,
SHEN Hong-xing
,
shenhongxing
2010, 31(7):739-743.
DOI: 10.3724/SP.J.1008.2010.0739
Abstract:
ObjectiveTo observe the pathological changes of nucleus pulposus(NP)in cervical spondylosis patients and the expression of phosphorylated p38 mitogen activated protein kinase(p-p38 MAPK) in NP with the progression of cervical disc degeneration, in an attempt to explore the mechanism behind cervical disc degeneration.MethodsNP specimens were collected from 35 patients with cervical spondylosis, who received surgical treatment during October 2008 to May 2009 in our hospital.The preoperative MRI (T2-weighted midsagittal images) information of all patients was obtained.The 35 cases included 25 males and 10 females, with an age range of 27-82 years.Five specimens were from a single space, 18 from two spaces, and 12 from three spaces; 4 specimens were from C3/4, 12 from C4/5,14 from C5/6, and 5 from C6/7.Nineteen specimens were used for pathology and immunohistochemistry study and 16 for Western blotting analysis.The degrees of disc degeneration were assessed by MRI grading system for cervical intervertebral disc degeneration.The pathological changes of NP of different degeneration grades and the location of p-p38 MAPK in NP were observed, and the relationship between the p-p38 MAPK and the degrees of MRI degeneration was analyzed.ResultsWith the aggravation of discs degeneration, the collagen fibers within NP were increased, thickened, and degraded, forming clusters, and the NP gradually became fibrotic.Phosphorylated p38 MAPK located in the cell nucleus of NP tissues.The positive rate of p-p38 MAPK increased gradually with the aggravation of degeneration, and the expression degrees of p-p38 MAPK (negative, weakly positive, strongly positive) were significantly different between groups of different degeneration degrees(P<0.05).A linear relationship was found between p-p38 MAPK expression and the degeneration degrees of cervical disc:Y=0.423X-0.675 (P<0.05).ConclusionNormal tissues are gradually replaced by fibrotic tissues in degenerated NP.Phosphorylated p38 MAPK is located in the cell nucleus of NP cells; the p-p38 MAPK plays important roles in the cervical intervertebral discs degeneration.Expression of phosphorylated p38 MAPK in NP increases with the aggravation of degeneration, suggesting that activation of p38 MAPK signaling pathway may be one of the mechanisms for cervical disc degeneration.
ObjectiveTo observe the pathological changes of nucleus pulposus(NP)in cervical spondylosis patients and the expression of phosphorylated p38 mitogen activated protein kinase(p-p38 MAPK) in NP with the progression of cervical disc degeneration, in an attempt to explore the mechanism behind cervical disc degeneration.MethodsNP specimens were collected from 35 patients with cervical spondylosis, who received surgical treatment during October 2008 to May 2009 in our hospital.The preoperative MRI (T2-weighted midsagittal images) information of all patients was obtained.The 35 cases included 25 males and 10 females, with an age range of 27-82 years.Five specimens were from a single space, 18 from two spaces, and 12 from three spaces; 4 specimens were from C3/4, 12 from C4/5,14 from C5/6, and 5 from C6/7.Nineteen specimens were used for pathology and immunohistochemistry study and 16 for Western blotting analysis.The degrees of disc degeneration were assessed by MRI grading system for cervical intervertebral disc degeneration.The pathological changes of NP of different degeneration grades and the location of p-p38 MAPK in NP were observed, and the relationship between the p-p38 MAPK and the degrees of MRI degeneration was analyzed.ResultsWith the aggravation of discs degeneration, the collagen fibers within NP were increased, thickened, and degraded, forming clusters, and the NP gradually became fibrotic.Phosphorylated p38 MAPK located in the cell nucleus of NP tissues.The positive rate of p-p38 MAPK increased gradually with the aggravation of degeneration, and the expression degrees of p-p38 MAPK (negative, weakly positive, strongly positive) were significantly different between groups of different degeneration degrees(P<0.05).A linear relationship was found between p-p38 MAPK expression and the degeneration degrees of cervical disc:Y=0.423X-0.675 (P<0.05).ConclusionNormal tissues are gradually replaced by fibrotic tissues in degenerated NP.Phosphorylated p38 MAPK is located in the cell nucleus of NP cells; the p-p38 MAPK plays important roles in the cervical intervertebral discs degeneration.Expression of phosphorylated p38 MAPK in NP increases with the aggravation of degeneration, suggesting that activation of p38 MAPK signaling pathway may be one of the mechanisms for cervical disc degeneration.
2009, 30(3):291-294.
DOI: 10.3724/SP.J.1008.2009.0291
Abstract:
Objective:To analyze the changes of the complementarity-determining region 3(CDR3) of T-cell receptor (TCR) beta chain variable region (TCR BV) in peripheral blood mononuclear cells (PBMCs) of patients with pemphigus vulgaris (PV), so as to understand the association of proliferated T cells with autoimmune diseases.Methods: Immunoscope spectratyping technique was used to analyze the distribution of TCR β chain CDR3 in 6 normal blood donors and the dominant CDR3 in the PBMCs in 6 PV patients. Results: The spectratypes of TCR BV subfamily CDR3 region was in a Gaussian distribution manner in all the 6 normal blood donors. The 6 PV patients, however, displayed abnormal proliferation, and oligoclonal expansion of the T cells was observed in TCR BV families with different CDR3 sequences. Conclusion: The abnormal spectratypes of TCR BV subfamily CDR3 region in the peripheral blood of PV patients might be related to the pathogenesis of PV.
Objective:To analyze the changes of the complementarity-determining region 3(CDR3) of T-cell receptor (TCR) beta chain variable region (TCR BV) in peripheral blood mononuclear cells (PBMCs) of patients with pemphigus vulgaris (PV), so as to understand the association of proliferated T cells with autoimmune diseases.Methods: Immunoscope spectratyping technique was used to analyze the distribution of TCR β chain CDR3 in 6 normal blood donors and the dominant CDR3 in the PBMCs in 6 PV patients. Results: The spectratypes of TCR BV subfamily CDR3 region was in a Gaussian distribution manner in all the 6 normal blood donors. The 6 PV patients, however, displayed abnormal proliferation, and oligoclonal expansion of the T cells was observed in TCR BV families with different CDR3 sequences. Conclusion: The abnormal spectratypes of TCR BV subfamily CDR3 region in the peripheral blood of PV patients might be related to the pathogenesis of PV.
2008, 29(9):1015-1019.
DOI: 10.3724/SP.J.1008.2008.01015
Abstract:
Objective:To investigate the effect of bone marrow mesenchymal stem cells (mMSC) infusion on the reconstruction of hematopoiesis function in mice with critical irradiation injury.Methods: Female C57BL/6 mice irradiated with 8 Gy 60Co γ-rays were randomly divided into control group and mMSCs-treated group.Mice in the mMSCs-treated group received mMSC of male C57BL/6 mice.The peripheral leukocyte,platelet,and erythrocyte counts,the pathological lesions,granulocyte-macrophage progenitors (CFU-GM),and sex chromosome ratio were all examined.Results: All the animals in the control group (n=6) died of hematopoiesis exhaustion 20 days after irradiation.The peripheral leukocyte of mMSC-treated group decreased greatly after bone marrow transplantation and quickly recovered 2 weeks later; till 28 days after transplantation,it recovered to 60% of the pre-irradiation level.mMSCs infusion promoted the quick recovery of the nucleated bone marrow cells and CFU-GM,improving the marrow morphology.The transplanted mMSC could be detected in the recipients 42 days after transplantation.Conclusion: Solo-injection of mMSCs can enhance hematopoietic reconstitution in mice with critical irradiation injury. The period of imbedding is related to the dose of infusion.
Objective:To investigate the effect of bone marrow mesenchymal stem cells (mMSC) infusion on the reconstruction of hematopoiesis function in mice with critical irradiation injury.Methods: Female C57BL/6 mice irradiated with 8 Gy 60Co γ-rays were randomly divided into control group and mMSCs-treated group.Mice in the mMSCs-treated group received mMSC of male C57BL/6 mice.The peripheral leukocyte,platelet,and erythrocyte counts,the pathological lesions,granulocyte-macrophage progenitors (CFU-GM),and sex chromosome ratio were all examined.Results: All the animals in the control group (n=6) died of hematopoiesis exhaustion 20 days after irradiation.The peripheral leukocyte of mMSC-treated group decreased greatly after bone marrow transplantation and quickly recovered 2 weeks later; till 28 days after transplantation,it recovered to 60% of the pre-irradiation level.mMSCs infusion promoted the quick recovery of the nucleated bone marrow cells and CFU-GM,improving the marrow morphology.The transplanted mMSC could be detected in the recipients 42 days after transplantation.Conclusion: Solo-injection of mMSCs can enhance hematopoietic reconstitution in mice with critical irradiation injury. The period of imbedding is related to the dose of infusion.
2010, 31(5):572-573.
DOI: 10.3724/SP.J.1008.2010.0572
Abstract:
查尔酮作为黄酮类化合物家族的一员,具有广泛的生物活性,如抗菌、抗氧化、降血脂、抗炎等作用[1-2]。尤其2’-羟基查尔酮是合成黄酮、黄酮醇以及二氢查尔酮衍生物等的重要中间体,也可用于香料和药物等精细化学品的合成。 目前关于取代查尔酮合成的报道不多,而且反应条件普遍苛刻,反应副产物多,收率低[3]。文献[4]报道了2’-羟基查尔酮的合成方法,我们采用该文献方法以带有不同供电子基的醛和酮缩合,结果重复性不高,且该文报道的产率均为粗产率。为寻找较适合于取代苯甲醛与邻羟基苯乙酮反应的工业化条件,本实验以藜芦醛和邻羟基苯乙酮反应为例（图1）,利用正交试验分析软件最优反应条件,考察在最优反应条件下不同取代的苯甲醛和邻羟基苯乙酮反应的情况。
查尔酮作为黄酮类化合物家族的一员,具有广泛的生物活性,如抗菌、抗氧化、降血脂、抗炎等作用[1-2]。尤其2’-羟基查尔酮是合成黄酮、黄酮醇以及二氢查尔酮衍生物等的重要中间体,也可用于香料和药物等精细化学品的合成。 目前关于取代查尔酮合成的报道不多,而且反应条件普遍苛刻,反应副产物多,收率低[3]。文献[4]报道了2’-羟基查尔酮的合成方法,我们采用该文献方法以带有不同供电子基的醛和酮缩合,结果重复性不高,且该文报道的产率均为粗产率。为寻找较适合于取代苯甲醛与邻羟基苯乙酮反应的工业化条件,本实验以藜芦醛和邻羟基苯乙酮反应为例（图1）,利用正交试验分析软件最优反应条件,考察在最优反应条件下不同取代的苯甲醛和邻羟基苯乙酮反应的情况。
2009, 30(3):283-285.
DOI: 10.3724/SP.J.1008.2009.0283
Abstract:
Objective:To investigate the reliability, validity and sensitivity of Chinese scale of clinical neurological deficit of stroke patients (China Stroke Scale, CSS), so as to assess its clinical application value.Methods: A total of 126 consecutive inpatients with acute stroke onset were included in our study and they were scored by CSS and the United States National Institutes of Health Stroke Scale (NIHSS) score separately; the reliability, validity, and sensitivity of CSS were evaluated. Reliability was evaluated by correlation coefficient r and Cronbach’s α coefficient; construct validity was analyzed by factor analysis method of appraisal; criterion validity was analyzed by the correlation coefficient analysis with NIHSS scale as the criterion. Sensitivity in various fields was assessed through standardization of effect (SES).Results: Totally 123 valid questionnaires were collected. CSS showed high intrarater reliability, interrater reliability (0.911-1.000) and good internal consistency, with the Cronbach’s α>0.8. There was concurrent validity between CSS and NIHSS (r=0.86). The prognosis prediction accuracy of CSS was 92.4%, slightly lower than that of NIHSS (94.1%). Logistic regression showed that CSS’s “gaze function” and “facial paralysis” were not included in the prediction equation. The facial paralysis had a SES of 0.38, all others had a SES higher than 0.5. Most fields showed a good sensitivity.Conclusion: CSS shows an acceptable reliability, validity and sensitivity in patients with stroke, but the predicative validity of CSS is inferior to that of NIHSS, which needs be further revised.
Objective:To investigate the reliability, validity and sensitivity of Chinese scale of clinical neurological deficit of stroke patients (China Stroke Scale, CSS), so as to assess its clinical application value.Methods: A total of 126 consecutive inpatients with acute stroke onset were included in our study and they were scored by CSS and the United States National Institutes of Health Stroke Scale (NIHSS) score separately; the reliability, validity, and sensitivity of CSS were evaluated. Reliability was evaluated by correlation coefficient r and Cronbach’s α coefficient; construct validity was analyzed by factor analysis method of appraisal; criterion validity was analyzed by the correlation coefficient analysis with NIHSS scale as the criterion. Sensitivity in various fields was assessed through standardization of effect (SES).Results: Totally 123 valid questionnaires were collected. CSS showed high intrarater reliability, interrater reliability (0.911-1.000) and good internal consistency, with the Cronbach’s α>0.8. There was concurrent validity between CSS and NIHSS (r=0.86). The prognosis prediction accuracy of CSS was 92.4%, slightly lower than that of NIHSS (94.1%). Logistic regression showed that CSS’s “gaze function” and “facial paralysis” were not included in the prediction equation. The facial paralysis had a SES of 0.38, all others had a SES higher than 0.5. Most fields showed a good sensitivity.Conclusion: CSS shows an acceptable reliability, validity and sensitivity in patients with stroke, but the predicative validity of CSS is inferior to that of NIHSS, which needs be further revised.
2010, 31(5):540-0.
DOI: 10.3724/SP.J.1008.2010.0540
Abstract:
1 临床资料 患者，女，27岁。因孕21+6周，右下腹痛伴不规律腹胀4 d就诊。入院前4 d出现右下腹痛，逐渐加重，伴下腹部不规律阵发性胀痛，无阴道流血、流液。2 d前于外院行B超示子宫右侧一约17 cm大小等回声团块，腹水最深4 cm；CA125、AFP轻度升高。诊断为“晚期先兆流产，盆腔肿瘤”，给予抑制宫缩治疗，症状未改善。急诊入我院后查体：右下腹压痛，可及一直径约15 cm包块，腹部移动性浊音阳性。产检：胎心162次/min，有宫缩，质弱，胎膜未破。B超示：子宫右后壁见15.5 cm×10.8 cm×11.6 cm囊实性团块，子宫直肠凹少量积液。CA125 104 U/ml，CRP 32.9 mg/L，AFP、CEA、CA199、CA15-3、SCC均未见异常。急查血常规：Hb 81 g/L。入院诊断：（1）孕21+6周，先兆早产；（2）盆腔包块待查：右侧卵巢肿瘤？给予保胎、对症治疗。入院后第3天晨，患者诉腹痛加重，复查血常规提示Hb降至64 g/L，B超提示盆腹腔积液增多，考虑内出血可能，急行剖腹探查术。术中见：腹腔大量积血及血凝块，总量约4 000 ml。右侧卵巢肿瘤包膜有一约5 cm破裂口，内有烂肉样组织流出，有活动性出血。给予输血、补液，行右侧附件切除术。术后病理：肿瘤细胞卵圆形，梭形，核大深染，显著异型，排列成片状旋涡状，部分区域疏松水肿，肿瘤内可见片状出血。免疫病理：VIM（+），SMA（部分+），Ki-67（），ER（+），PR（+），CK8/18（-），EMA（-），S-100（-）。结合形态学初步诊断：（右卵巢）恶性性索间质肿瘤,疑为未分类性索间质肿瘤或纤维肉瘤。术后给予抗炎保胎对症治疗7 d，患者病情平稳，胎儿情况未提示异常。建议患者引产后行化疗，但患者拒绝，要求出院。
1 临床资料 患者，女，27岁。因孕21+6周，右下腹痛伴不规律腹胀4 d就诊。入院前4 d出现右下腹痛，逐渐加重，伴下腹部不规律阵发性胀痛，无阴道流血、流液。2 d前于外院行B超示子宫右侧一约17 cm大小等回声团块，腹水最深4 cm；CA125、AFP轻度升高。诊断为“晚期先兆流产，盆腔肿瘤”，给予抑制宫缩治疗，症状未改善。急诊入我院后查体：右下腹压痛，可及一直径约15 cm包块，腹部移动性浊音阳性。产检：胎心162次/min，有宫缩，质弱，胎膜未破。B超示：子宫右后壁见15.5 cm×10.8 cm×11.6 cm囊实性团块，子宫直肠凹少量积液。CA125 104 U/ml，CRP 32.9 mg/L，AFP、CEA、CA199、CA15-3、SCC均未见异常。急查血常规：Hb 81 g/L。入院诊断：（1）孕21+6周，先兆早产；（2）盆腔包块待查：右侧卵巢肿瘤？给予保胎、对症治疗。入院后第3天晨，患者诉腹痛加重，复查血常规提示Hb降至64 g/L，B超提示盆腹腔积液增多，考虑内出血可能，急行剖腹探查术。术中见：腹腔大量积血及血凝块，总量约4 000 ml。右侧卵巢肿瘤包膜有一约5 cm破裂口，内有烂肉样组织流出，有活动性出血。给予输血、补液，行右侧附件切除术。术后病理：肿瘤细胞卵圆形，梭形，核大深染，显著异型，排列成片状旋涡状，部分区域疏松水肿，肿瘤内可见片状出血。免疫病理：VIM（+），SMA（部分+），Ki-67（），ER（+），PR（+），CK8/18（-），EMA（-），S-100（-）。结合形态学初步诊断：（右卵巢）恶性性索间质肿瘤,疑为未分类性索间质肿瘤或纤维肉瘤。术后给予抗炎保胎对症治疗7 d，患者病情平稳，胎儿情况未提示异常。建议患者引产后行化疗，但患者拒绝，要求出院。
2009, 30(6):628-631.
DOI: 10.3724/SP.J.1008.2009.00
Abstract:
Objective:To elucidate the genetic characteristics and deduced protein variation of nonstructural protein(NS)gene of the novel influenza virus A/H1N1 in 2009 pandemic.Methods: The sequence of NS gene of A/H1N1 viruses isolated in North America,Europe,and Asia during 1930-2009 were downloaded from NCBI database.MEGA4.0 software and NJ method were used for sequence alignment,protein sequence alignment,and the phylogenetic tree construction.Results: The NS genes of novel influenza virus A/H1N1 in 2009 pandemic were originated from A/swine/H1N1 virus of 2005-2007; they shared a high homology of 97.5％-97.6%.There was an obvious evolutionary relationship between the NS genes of novel A/H1N1 virus and those of the influenza A/swine/H1N1 viruses isolated in North America from 1930 to 2007.No obvious changes were found in the amino acid sites for the antagonistic function of NS1 against the host antiviral capacity among these viruses.Conclusion: The NS gene of novel influenza virus A/H1N1 in 2009 pandemic might evolve from swine A/H1N1 influenza viruses isolated in the United States.The antagonistic function of NS1 against the host antiviral capacity is not changed.
Objective:To elucidate the genetic characteristics and deduced protein variation of nonstructural protein(NS)gene of the novel influenza virus A/H1N1 in 2009 pandemic.Methods: The sequence of NS gene of A/H1N1 viruses isolated in North America,Europe,and Asia during 1930-2009 were downloaded from NCBI database.MEGA4.0 software and NJ method were used for sequence alignment,protein sequence alignment,and the phylogenetic tree construction.Results: The NS genes of novel influenza virus A/H1N1 in 2009 pandemic were originated from A/swine/H1N1 virus of 2005-2007; they shared a high homology of 97.5％-97.6%.There was an obvious evolutionary relationship between the NS genes of novel A/H1N1 virus and those of the influenza A/swine/H1N1 viruses isolated in North America from 1930 to 2007.No obvious changes were found in the amino acid sites for the antagonistic function of NS1 against the host antiviral capacity among these viruses.Conclusion: The NS gene of novel influenza virus A/H1N1 in 2009 pandemic might evolve from swine A/H1N1 influenza viruses isolated in the United States.The antagonistic function of NS1 against the host antiviral capacity is not changed.
2009, 30(4):442-446.
DOI: 10.3724/SP.J.1008.2009.0442
Abstract:
The discovery of circulating fetal DNA paves a new way for non-invasive prenatal diagnosis. Examining the specific fetal DNA sequence of the circulating fetal DNA has been used for prenatal diagnosis of sex-linked disorders, fetal rhesus D blood typing and single gene inheritance disease. The variation of the circulating DNA levels can also be used for diagnosis of preeclampsia, premature delivery, and fetal chromosome disorders. This paper aims to review the biochemistry characters and clinical application of the circulating fetal DNA in maternal peripheral plasma.
The discovery of circulating fetal DNA paves a new way for non-invasive prenatal diagnosis. Examining the specific fetal DNA sequence of the circulating fetal DNA has been used for prenatal diagnosis of sex-linked disorders, fetal rhesus D blood typing and single gene inheritance disease. The variation of the circulating DNA levels can also be used for diagnosis of preeclampsia, premature delivery, and fetal chromosome disorders. This paper aims to review the biochemistry characters and clinical application of the circulating fetal DNA in maternal peripheral plasma.
TAN Hong-wen
,
ZHANG Zhi-gang
,
BAI Yuan
,
LI Chang-yong
,
XU Xu-dong
,
ZHANG Bi-li
,
ZHU Jia-qi
,
ZHAO Xian-xian
,
WU Hong
,
QIN Yong-wen
2014, 35(3):317-320.
DOI: 10.3724/SP.J.1008.2014.00317
Abstract:
Objective To assess the safety and effectiveness of self-made side hole balloon for treating no-reflow following percutaneous coronary intervention(PCI). Methods Twenty-three patients diagnosed with no-reflow during PCI from Jan. 2012 to Jan. 2013 were enrolled. Residual stenosis, thrombosis,dissection, and spasm of coronary artery were excluded. The mean age of the 23 patients was (62.0±13.8) years old. Of the 23 patients 14 had ST segment elevation myocardial infarction (STEMI), and 9 underwent elective PCI. There were 11 cases with no-reflow in the left anterior descending branch, 8 in the right coronary artery and 4 in the circumflex branch. The drugs (nitroglycerin and tirofiban) were selectively injected into the vessel using self-made side hole balloons. The thrombolysis in myocardial infarction (TIMI) grade before and after procedure,ST segment resolution (STR),ST-T changes for 24 and 72 hours and complications (perforation, dissection,and thrombosis of coronary artery) were observed postoperatively. Patients were followed up by ECG and echocardiogram at 1 month after PCI. Incidence of major adverse cardiovascular events (MACEs) and cardiac function were observed 6 months after PCI. Results After intracoronary administration of drug therapy, TIMI grade-1 flow was found in 3 patients, TIMI grade-2 flow in 5 patients and TIMI grade-3 flow in 15 patients. In patients with STEMI, complete resolution (≥70%) was found in 8 patients,partial resolution (30%-69%) in 4, and no resolution (<30%) in 2. One of the 2 patients with ventricular electrical storm was treated with temporary cardiac pacing and drug therapy and recovered within 1 week; the other one with pericardial tamponade who was treated with emergency surgery repair died. In 9 patients undergoing selected PCI,transient ST segment changes were noted in 3 patients which recovered within 1 week after conservative treatment; with no perforation, dissection,or thrombosis of coronary artery. One month after discharge, echocardiogram of the 22 patients showed a mean left ventricular ejection fraction (LÜEF) of (50.6±14.3)% and a fractional shortening in the short axis view of 0.36±0.04, and ECG showed non-specific changes of ST-T in 6 patients. In 4 patients with STEMI, culprit artery showed TIMI grade-3 by angiography performed during PCI for non-culprit vessel 1 month after primary PCI. At 6 months after primary PCI, there was no MACE; 18 patients were in New York Heart Association Class Ⅰ and 4 in Class Ⅱ. Conclusion Self-made side hole balloon is a safe, economical, effective and convenient method for intracoronary administration of nitroglycerin and tirofiban in treating no-flow during PCI, but the result still needs further verification.
Objective To assess the safety and effectiveness of self-made side hole balloon for treating no-reflow following percutaneous coronary intervention(PCI). Methods Twenty-three patients diagnosed with no-reflow during PCI from Jan. 2012 to Jan. 2013 were enrolled. Residual stenosis, thrombosis,dissection, and spasm of coronary artery were excluded. The mean age of the 23 patients was (62.0±13.8) years old. Of the 23 patients 14 had ST segment elevation myocardial infarction (STEMI), and 9 underwent elective PCI. There were 11 cases with no-reflow in the left anterior descending branch, 8 in the right coronary artery and 4 in the circumflex branch. The drugs (nitroglycerin and tirofiban) were selectively injected into the vessel using self-made side hole balloons. The thrombolysis in myocardial infarction (TIMI) grade before and after procedure,ST segment resolution (STR),ST-T changes for 24 and 72 hours and complications (perforation, dissection,and thrombosis of coronary artery) were observed postoperatively. Patients were followed up by ECG and echocardiogram at 1 month after PCI. Incidence of major adverse cardiovascular events (MACEs) and cardiac function were observed 6 months after PCI. Results After intracoronary administration of drug therapy, TIMI grade-1 flow was found in 3 patients, TIMI grade-2 flow in 5 patients and TIMI grade-3 flow in 15 patients. In patients with STEMI, complete resolution (≥70%) was found in 8 patients,partial resolution (30%-69%) in 4, and no resolution (<30%) in 2. One of the 2 patients with ventricular electrical storm was treated with temporary cardiac pacing and drug therapy and recovered within 1 week; the other one with pericardial tamponade who was treated with emergency surgery repair died. In 9 patients undergoing selected PCI,transient ST segment changes were noted in 3 patients which recovered within 1 week after conservative treatment; with no perforation, dissection,or thrombosis of coronary artery. One month after discharge, echocardiogram of the 22 patients showed a mean left ventricular ejection fraction (LÜEF) of (50.6±14.3)% and a fractional shortening in the short axis view of 0.36±0.04, and ECG showed non-specific changes of ST-T in 6 patients. In 4 patients with STEMI, culprit artery showed TIMI grade-3 by angiography performed during PCI for non-culprit vessel 1 month after primary PCI. At 6 months after primary PCI, there was no MACE; 18 patients were in New York Heart Association Class Ⅰ and 4 in Class Ⅱ. Conclusion Self-made side hole balloon is a safe, economical, effective and convenient method for intracoronary administration of nitroglycerin and tirofiban in treating no-flow during PCI, but the result still needs further verification.
1999, 20(4).
Abstract:
Objective: To investigate the effects of oxymatrin (OM) and glycyrrhizin (GL) on hepatocyte apoptosis induced by cessation of phenobarbital(PB) treatment in mice. Methods: The Kunming mice were treated with OM (150mg/kg,ip) and GL (50mg/kg,ip) 3times daily during 36h after cessation of PB treatment. Hepatic DNA content and the ratio of liver/body mass of mice were observed to estimate the regressive rate of hyperplastic liver at 36h after withdrawing PB. Histomorphology and the end-labeling method termed TUNEL (TdT-mediated X-dUTP nick end labeling) were used to identify apoptotic cells and DNA in apoptotic cells. Results: Regression ratio of hepatic DNA content and liver/body mass in the positive control group was 32.4%and 16.3%, and 28.9%and 16.1%in the group treated with GL. Hepatic DNA content and liver/body mass in the group treated with OM produced little regression as compared with the negative control group. The results showed that OM markedly inhibited the regression of hepatic DNA content and liver/body mass, but GL showed no effect. Additionally, those mice in the positive control group and the group treated with GL produced a typical hepatocyte apoptotic change with a positive result of TUNEL assay. No hepatocyte apoptotic changes were found in the liver of mice treated with OM, and a negative result of TUNEL assay was presented. Conclusion: OM inhibits the regression of hepatic DNA content and liver/body mass by preventing hepatocyte apoptosis induced by cessation of PB treatment in mice.
Objective: To investigate the effects of oxymatrin (OM) and glycyrrhizin (GL) on hepatocyte apoptosis induced by cessation of phenobarbital(PB) treatment in mice. Methods: The Kunming mice were treated with OM (150mg/kg,ip) and GL (50mg/kg,ip) 3times daily during 36h after cessation of PB treatment. Hepatic DNA content and the ratio of liver/body mass of mice were observed to estimate the regressive rate of hyperplastic liver at 36h after withdrawing PB. Histomorphology and the end-labeling method termed TUNEL (TdT-mediated X-dUTP nick end labeling) were used to identify apoptotic cells and DNA in apoptotic cells. Results: Regression ratio of hepatic DNA content and liver/body mass in the positive control group was 32.4%and 16.3%, and 28.9%and 16.1%in the group treated with GL. Hepatic DNA content and liver/body mass in the group treated with OM produced little regression as compared with the negative control group. The results showed that OM markedly inhibited the regression of hepatic DNA content and liver/body mass, but GL showed no effect. Additionally, those mice in the positive control group and the group treated with GL produced a typical hepatocyte apoptotic change with a positive result of TUNEL assay. No hepatocyte apoptotic changes were found in the liver of mice treated with OM, and a negative result of TUNEL assay was presented. Conclusion: OM inhibits the regression of hepatic DNA content and liver/body mass by preventing hepatocyte apoptosis induced by cessation of PB treatment in mice.
1999, 20(7).
Abstract:
Objective： To evaluate the effects of oxymatrine on liver fibrosis. Methods: Chronic liver damage and liver fibrosis in rats were induced by hypodermically injecting carbon tetrachloride (CCl4) twice a week for 12 weeks, and were simultaneously treated intraperitoneally with oxymatrine once a day. The levels of serum alanine aminotransferase (ALT), type IV collagen (Ⅳ-C), hyaluronate (HA), tumor necrosis factor alpha (TNFα) were detected at various times, and the degrees of inflammatory activity and fibrogenesis in liver tissues were also observed. Results: The levels of serum ALT, IV-C, HA, and TNFα were lower in the oxymatrine-treated rats than those in the oxymatrine-untreated ones, and so were the degrees of inflammatory activity and fibrogenesis in liver tissues. Conclusion: Oxymatrine can inhibit the inflammatory activity and fibrogenesis in liver tissue.
Objective： To evaluate the effects of oxymatrine on liver fibrosis. Methods: Chronic liver damage and liver fibrosis in rats were induced by hypodermically injecting carbon tetrachloride (CCl4) twice a week for 12 weeks, and were simultaneously treated intraperitoneally with oxymatrine once a day. The levels of serum alanine aminotransferase (ALT), type IV collagen (Ⅳ-C), hyaluronate (HA), tumor necrosis factor alpha (TNFα) were detected at various times, and the degrees of inflammatory activity and fibrogenesis in liver tissues were also observed. Results: The levels of serum ALT, IV-C, HA, and TNFα were lower in the oxymatrine-treated rats than those in the oxymatrine-untreated ones, and so were the degrees of inflammatory activity and fibrogenesis in liver tissues. Conclusion: Oxymatrine can inhibit the inflammatory activity and fibrogenesis in liver tissue.
2009, 30(6):610-612.
DOI: 10.3724/SP.J.1008.2009.0610
Abstract:
Since the first reported case of swine influenza A in Mexico, a total of 15 510 cases have been confirmed in 53 countries by May 29, 2009.On April 29, 2009, the World Health Organization (WHO) raised its pandemic alert from grade 4 to grade 5.The virus is described as a new subtype of A/H1N1 and is not detected in pigs or humans previously.The virus is sensitive to oseltamivir and zanamivir, but resistant to amantadine and rimantadine.The genetics of the virus are so novel that humans are unlikely to have much immunity to it.The virus can transmit from human to human; therefore it is necessary to enforce quarantine measure for close contactors because the virus transmits during latency.Precaution methods like covering noses and mouths with a tissue when coughing or sneezing can reduce the transmission opportunity.Hands should be washed frequently with soap, especially after coughing or sneezing.Public places with ventilation conditions, personal health behavior and health condition are critical for the prevention and control of this epidemic.
Since the first reported case of swine influenza A in Mexico, a total of 15 510 cases have been confirmed in 53 countries by May 29, 2009.On April 29, 2009, the World Health Organization (WHO) raised its pandemic alert from grade 4 to grade 5.The virus is described as a new subtype of A/H1N1 and is not detected in pigs or humans previously.The virus is sensitive to oseltamivir and zanamivir, but resistant to amantadine and rimantadine.The genetics of the virus are so novel that humans are unlikely to have much immunity to it.The virus can transmit from human to human; therefore it is necessary to enforce quarantine measure for close contactors because the virus transmits during latency.Precaution methods like covering noses and mouths with a tissue when coughing or sneezing can reduce the transmission opportunity.Hands should be washed frequently with soap, especially after coughing or sneezing.Public places with ventilation conditions, personal health behavior and health condition are critical for the prevention and control of this epidemic.
2008, 29(8):0990-0993.
DOI: 10.3724/SP.J.1008.2008.00990
Abstract:
目的：观察创伤性脑水肿合并海水淹溺后大鼠血清、脑组织、肺组织IL-1β和TNF-α含量的变化,探讨其变化规律及临床意义。方法:脑侧方液压打击伤加气管内灌注海水建立大鼠创伤性脑水肿合并海水淹溺模型。伤后1、6、12、24及48 h检测大鼠脑、肺组织含水量及脑、肺组织匀浆和血清中IL-1β、TNF-α的含量;观察伤后24 h脑、肺组织病理学变化。以颅脑创伤合并淡水淹溺、单纯颅脑创伤、单纯海水淹溺、假手术大鼠作为对照。结果:脑损伤合并海水淹溺后,大鼠脑组织含水量较单纯脑损伤、单纯海水淹溺以及脑损伤合并淡水淹溺均显著增高(P＜0.05);脑组织、肺组织IL-1β和TNF-α含量有显著变化。脑、肺组织病理学损伤明显。结论:海水的损伤性作用是重要致伤因素之一,炎症反应在创伤性脑水肿合并海水淹溺性肺水肿的病理生理过程中起了重要作用。
目的：观察创伤性脑水肿合并海水淹溺后大鼠血清、脑组织、肺组织IL-1β和TNF-α含量的变化,探讨其变化规律及临床意义。方法:脑侧方液压打击伤加气管内灌注海水建立大鼠创伤性脑水肿合并海水淹溺模型。伤后1、6、12、24及48 h检测大鼠脑、肺组织含水量及脑、肺组织匀浆和血清中IL-1β、TNF-α的含量;观察伤后24 h脑、肺组织病理学变化。以颅脑创伤合并淡水淹溺、单纯颅脑创伤、单纯海水淹溺、假手术大鼠作为对照。结果:脑损伤合并海水淹溺后,大鼠脑组织含水量较单纯脑损伤、单纯海水淹溺以及脑损伤合并淡水淹溺均显著增高(P＜0.05);脑组织、肺组织IL-1β和TNF-α含量有显著变化。脑、肺组织病理学损伤明显。结论:海水的损伤性作用是重要致伤因素之一,炎症反应在创伤性脑水肿合并海水淹溺性肺水肿的病理生理过程中起了重要作用。
2009, 30(11):1292-1296.
DOI: 10.3724/SP.J.1008.2009.01292
Abstract:
Objective:To identify the components for nurse job satisfaction,and to develop a scale for job satisfaction assessment in Chinese nurses.Methods:Using literature review and expert consulting,we developed a primary scale consisting of 10 factors and 56 items.After testing with 135 nurses,a nurse job satisfaction scale composing of 8 factors and 38 items was developed,and was used to test 1 185 nurses; the reliability and efficacy of the scale were also assessed.Results:The developed nurse job satisfaction scale was composed of 8 factors (administration,work loads,relationship with colleagues,job,salary and welfare,professional opportunities,work recognition by others,and balance between family and work) and 38 items,and it had good reliability and efficacy.Conclusion:The developed scale can not only help to evaluate job satisfaction among nurses,but also provide evidence for corresponding intervention.
Objective:To identify the components for nurse job satisfaction,and to develop a scale for job satisfaction assessment in Chinese nurses.Methods:Using literature review and expert consulting,we developed a primary scale consisting of 10 factors and 56 items.After testing with 135 nurses,a nurse job satisfaction scale composing of 8 factors and 38 items was developed,and was used to test 1 185 nurses; the reliability and efficacy of the scale were also assessed.Results:The developed nurse job satisfaction scale was composed of 8 factors (administration,work loads,relationship with colleagues,job,salary and welfare,professional opportunities,work recognition by others,and balance between family and work) and 38 items,and it had good reliability and efficacy.Conclusion:The developed scale can not only help to evaluate job satisfaction among nurses,but also provide evidence for corresponding intervention.
2007, 28(3):0306-0309.
DOI: 10.3724/SP.J.1008.2007.00306
Abstract:
Objectlve：To assess the clinical outcome of autologous fat injection in treatment of hemifacial atrophy, so as to search for an ideal treatment of hemifacial atrophy. Methods： Autologous fat granules were obtained by tumescent liposuction. After purification, the fat granules were injected in a multi-strata, multi-tunnel, and multi-spot manner to repair facial introcession abnormality. Patients whose introcession was not improved after the stabilization of the fillings were re-injected with autologous fat granules twice or more until satisfactory outcomes were achieved. Results： All the 7 patients in this study obtained satisfactory outcomes after fat injection and basically reached symmetry. The follow-up result was good after 2 years. Conclusion： Local injection transplantation of autologous fat granules is effective for the treatment of facial depression and hemifacial atrophy, with slight trauma, little complication, and satisfactory outcome.[
Objectlve：To assess the clinical outcome of autologous fat injection in treatment of hemifacial atrophy, so as to search for an ideal treatment of hemifacial atrophy. Methods： Autologous fat granules were obtained by tumescent liposuction. After purification, the fat granules were injected in a multi-strata, multi-tunnel, and multi-spot manner to repair facial introcession abnormality. Patients whose introcession was not improved after the stabilization of the fillings were re-injected with autologous fat granules twice or more until satisfactory outcomes were achieved. Results： All the 7 patients in this study obtained satisfactory outcomes after fat injection and basically reached symmetry. The follow-up result was good after 2 years. Conclusion： Local injection transplantation of autologous fat granules is effective for the treatment of facial depression and hemifacial atrophy, with slight trauma, little complication, and satisfactory outcome.[
2009, 30(8):968-969.
DOI: 10.3724/SP.J.1008.2009.0968
Abstract:
目的:探讨主动脉夹层致猝死的临床病理特点、发病机制及危险因素,提高其早期诊断及防治水平,降低病死率。方法:对2002-2007年7例主动脉夹层猝死病例进行临床尸检病理分析,总结其临床病理特点,分析发病机制及危险因素。结果:7例主动脉夹层猝死患者50岁以上4例,40岁以下3例,年龄30～71岁,平均（50.5±15）岁；多有主动脉粥样硬化和（或）高血压病史；临床表现最初大多为非心血管疾病症状。结论:主动脉夹层致病因素复杂,起病隐匿,常导致猝死；伴有长期高血压病、主动脉粥样硬化等高危人群出现突发性胸痛或休克表现时应警惕该病的发生并进行早期干预处理,降低患者病死率。
目的:探讨主动脉夹层致猝死的临床病理特点、发病机制及危险因素,提高其早期诊断及防治水平,降低病死率。方法:对2002-2007年7例主动脉夹层猝死病例进行临床尸检病理分析,总结其临床病理特点,分析发病机制及危险因素。结果:7例主动脉夹层猝死患者50岁以上4例,40岁以下3例,年龄30～71岁,平均（50.5±15）岁；多有主动脉粥样硬化和（或）高血压病史；临床表现最初大多为非心血管疾病症状。结论:主动脉夹层致病因素复杂,起病隐匿,常导致猝死；伴有长期高血压病、主动脉粥样硬化等高危人群出现突发性胸痛或休克表现时应警惕该病的发生并进行早期干预处理,降低患者病死率。
DENG Xiao-dong
,
YANG Zhi-gang
,
ZHANG Lei
,
HUANG Qing-hai
,
XU Yi
,
ZHAO Wen-yuan
,
LIU Jian-min
,
HONG Bo
2013, 34(5):521-525.
DOI: 10.3724/SP.J.1008.2013.00521
Abstract:
Objective To evaluate the feasibility, safety and efficacy of Solitaire stent placement for treatment of complex intracranial atherosclerotic stenosis. Methods We retrospectively reviewed the clinical data of 18 patients who underwent Solitaire stent placement for symptomatic intracranial arterial stenoses in our department between November 2011 and June 2012, with focus on the stenosis morphology, condition of the diseased blood vessels, surgical success rate, and perioperative complications. We also summarized the early outcomes and imaging findings during follow-up. Results The patients included 8 males and 10 females, with a mean age of 66.55±9.84 years old. Mori typing results were 5 A, 8 B, and 5 C types; LMA typing results were good in 2, moderate tortuosity in 12, severe tortuosity in 4 cases. All the patients received successful Solitaire stent implantation. The average stenosis rate was reduced from (74±12.5)％ to (10±5.5)% after the procedure. There was only one patient (5.6%) had post-operative complication (cerebral infarction of the target vessel territory)，who had temporal symptom and recovered well at follow-up.Clinical follow-up was available for 18 patients, with a mean of 6.2± 1.4 months; and no patients experienced any ischemic episodes. Fifteen patients had DSA or CTA follow-up for a mean of 7.8±2.6 months. There were 3 in-stent restenosis (20%) cases and one of them was symptomatic with transient ischemic attack. Conclusion Solitaire stent placement is feasible, safe and effective for complex symptomatic intracranial artery stenosis, with definite short-term effectiveness, and the long-term effect warrants further investigation.
Objective To evaluate the feasibility, safety and efficacy of Solitaire stent placement for treatment of complex intracranial atherosclerotic stenosis. Methods We retrospectively reviewed the clinical data of 18 patients who underwent Solitaire stent placement for symptomatic intracranial arterial stenoses in our department between November 2011 and June 2012, with focus on the stenosis morphology, condition of the diseased blood vessels, surgical success rate, and perioperative complications. We also summarized the early outcomes and imaging findings during follow-up. Results The patients included 8 males and 10 females, with a mean age of 66.55±9.84 years old. Mori typing results were 5 A, 8 B, and 5 C types; LMA typing results were good in 2, moderate tortuosity in 12, severe tortuosity in 4 cases. All the patients received successful Solitaire stent implantation. The average stenosis rate was reduced from (74±12.5)％ to (10±5.5)% after the procedure. There was only one patient (5.6%) had post-operative complication (cerebral infarction of the target vessel territory)，who had temporal symptom and recovered well at follow-up.Clinical follow-up was available for 18 patients, with a mean of 6.2± 1.4 months; and no patients experienced any ischemic episodes. Fifteen patients had DSA or CTA follow-up for a mean of 7.8±2.6 months. There were 3 in-stent restenosis (20%) cases and one of them was symptomatic with transient ischemic attack. Conclusion Solitaire stent placement is feasible, safe and effective for complex symptomatic intracranial artery stenosis, with definite short-term effectiveness, and the long-term effect warrants further investigation.
2008, 29(1):0083-0086.
DOI: 10.3724/SP.J.1008.2008.00083
Abstract:
Objective：To separate the nematocyst venom (NV) and tentacle-only extract (TOE) from the jellyfish Cyanea capillata and to analyze the difference between their haemolytic activities and the influencing factors.Methods: NV and TOE were separated by autolysis and centrifugation.The influence of concentration, temperature and pH value on the haemolytic activities of NV and TOE were observed.Results: NV and TOE were successfully separated.The concentration-associated haemolytic curves were “S” shaped for both NV and TOE.The HU50 of NV and TOE were 8 μg/ml and 67 μg/ml, respectively; and the haemolysis of NV was about 8.4 times of that of TOE.Temperature had great influence on haemolysis of both and the highest haemolysis were both at 40℃.pH value also had influence on haemolysis.The strongest haemolysis activity was found both at pH 8 and TOE was more sensitive.Conclusion: NV and TOE both have haemolysis activity,with the activity of NV stronger than that of the latter.The haemolysis activity is influenced by concentration, temperature and pH value.
Objective：To separate the nematocyst venom (NV) and tentacle-only extract (TOE) from the jellyfish Cyanea capillata and to analyze the difference between their haemolytic activities and the influencing factors.Methods: NV and TOE were separated by autolysis and centrifugation.The influence of concentration, temperature and pH value on the haemolytic activities of NV and TOE were observed.Results: NV and TOE were successfully separated.The concentration-associated haemolytic curves were “S” shaped for both NV and TOE.The HU50 of NV and TOE were 8 μg/ml and 67 μg/ml, respectively; and the haemolysis of NV was about 8.4 times of that of TOE.Temperature had great influence on haemolysis of both and the highest haemolysis were both at 40℃.pH value also had influence on haemolysis.The strongest haemolysis activity was found both at pH 8 and TOE was more sensitive.Conclusion: NV and TOE both have haemolysis activity,with the activity of NV stronger than that of the latter.The haemolysis activity is influenced by concentration, temperature and pH value.
