Objective To explore the correlation between stroke etiology and clinical and imaging features in patients with acute ischemic stroke (AIS) due to large vessel occlusion treated by intravascular thrombectomy. Methods A total of 213 patients with AIS and endovascular embolectomy in our hospital from Oct. 2016 to Jun. 2018 were enrolled retrospectively. According to the etiological classification criteria of Trial of Org 10172 in Acute Stroke Treatment (TOAST), there were 116 cases of cardioembolism and 97 cases of non-cardioembolism. Multivariate logistic regression analysis was used to screen the clinical and imaging characteristics for identifying cardioembolism and non-cardioembolism. Results Compared with non-cardioembolism AIS, cardioembolism AIS was associated with higher NIHSS scores (adjusted odds ratio [OR]＝1.09, 95% confidence interval [95%CI] 1.01-1.18, P＝0.02), atrial fibrillation (adjusted OR＝76.46, 95%CI 26.75-218.51, P＜0.01), absence of hypertension (adjusted OR＝0.32, 95%CI 0.12-0.84, P＝0.02), antiplatelet drug use (adjusted OR＝5.03, 95%CI 1.22-20.63, P＝0.03), shorter onset-to-puncture time (adjusted OR＝0.998, 95%CI 0.996-1.000, P＝0.04), and presence of hyperdense artery sign (HAS) (adjusted OR＝4.45, 95%CI 1.47-13.49, P＝0.01). Conclusion There are some differences in clinical and imaging characteristics between patients with cardioembolism and non-cardioembolism AIS. The occurrence of HAS suggests a higher probability of cardioembolism in AIS patients.