Objective To investigate the safety and feasibility of the domestic SA-1000 single-port single-arm robot-assisted laparoscopic system in total hysterectomy. Methods Data from 16 patients who underwent total hysterectomy using the SA-1000 system at the Department of Obstetrics and Gynecology, The Second Affiliated Hospital of Naval Medical University, between Mar. 2023 and Jan. 2024 were retrospectively collected. Surgical parameters were analyzed. Postoperative pain was assessed using the visual analogue scale (VAS) at 24 h after surgery and before discharge. Incision cosmesis was evaluated 3-5 weeks postoperatively using the body image questionnaire (BIQ, score range 3-24). Results All 16 procedures were successfully completed using the SA-1000 system without conversion to open surgery, achieving a 100.0% procedural success rate. The mean whole surgery time was (234.40±56.24) min. The median robotic arm setup time was 8.0 (4.0, 13.5) min, and the median console operating time was 128.0 (100.0, 151.0) min. The median intraoperative blood loss was 100.0 (100.0, 200.0) mL. No perioperative complications, such as hemorrhage, infection, injury to adjacent organs (ureters, bladder, bowel), poor wound healing, or incisional hernia, were observed. The mean wound pain score at 24 h postoperatively was 3.81±1.64, decreasing to a median of 3.0 (2.0, 4.0) before discharge. The BIQ score assessed at 3-5 weeks postoperatively was 21.88±1.15. Conclusion The application of the domestic SA-1000 single-port single-arm robot-assisted laparoscopic system for total hysterectomy is safe and feasible, demonstrating favorable surgical outcomes. It holds promise for broader implementation and promotion in domestic medical centers.