Objective To summarize the operation experience of 1 case of ultra-long-distance single-port robotic-assisted laparoscopic renal cyst decortication using a domestic robotic system, and provide references for the development of long-distance telesurgery. Methods The procedure was performed using a domestically produced single-port robotic system. The distance between the main control end and the patient end was 2 400 km. The operation was conducted remotely via a 5G network and a 100 Mbps dedicated line provided by China Telecom. The surgeon controlled the robotic arm remotely. Results The surgery was completed smoothly within a duration of 32 min. The real-time latency was (90±20) ms. Blood loss during the operation was 10 mL, and no drainage tube was placed. The patient recovered smoothly and returned to the ward without any complications. Conclusion It is safe and feasible for the domestically produced single-port robotic system to implement ultra-long-distance telesurgery. This approach facilitates the decentralization of advanced medical resources and helps mitigate disparities in healthcare access.