Motion sickness is a physiological response triggered by abnormal motion stimulation, presenting with gastric discomfort, nausea, vomiting, dizziness, and headache. Assessment of the severity of motion sickness and the efficacy of interventions is critical for developing more rational and effective therapeutic approaches and adaptive behavioral training protocols. In addition to objective evaluation of physiological indicators such as nystagmus, electroencephalogram, electrogastrogram, electrodermal activity, heart rate variability, postural sway, and hormone levels, assessment scales for susceptibility and subjective symptoms have increasingly gained attention from researchers worldwide in clinical research and practice. This review summarizes the research progress on physiological indicators of motion sickness, as well as the application of common assessment scales, providing a reference for screening, evaluation, prevention, and intervention in motion sickness.