| 摘要: |
| 目的:观察过氧化物酶体增殖物激活受体(PPAR)γ高表达对小鼠肾脏内髓集合管上皮细胞(IMCD)炎性刺激的反应.方法:实验分为4组:IMCD空白对照组,IMCD+TNFα刺激组,转染PPARγ野生型质粒的转染空白组(PPARγ-IMCD组)和PPARγ-IMCD+TNFα刺激组.TNFα刺激:采用TNFα 50 ng/ml作用24 h;质粒转染:将小鼠野生型PPARγ1质粒转染于IMCD细胞使PPARγ高表达.ELISA方法检测细胞上清液MCP-1、TGFβ1分泌量.结果:转染PPARγ野生型质粒的IMCD细胞PPARγ mRNA和蛋白高表达.1MCD空白对照组和PPARγ-IMCD转染空白组相比,细胞上清液MCP-1和TGFβ1含量无显著性差异;IMCD+TNFα刺激组培养上清液中MCP-1和TGF-β1表达明显增加(与IMCD空白对照组相比,P＜0.005),PPARγ-IMCD+TNFα刺激组培养上清液中MCP-1和TGFβ1的分泌与IMCD+TNFα刺激组相比显著减少(P＜0.05和P＜0.005).结论:IMCD细胞转染PPARγ野生型质粒使得PPARγ高表达后具有抗炎和抗纤维化作用. |
| 关键词: 过氧化物酶体增殖物激活受体γ、肿瘤坏死因子α、肾小管上皮细胞 |
| DOI：10.3724/SP.J.1008.2006.00599 |
| 投稿时间：2005-12-29修订日期：2006-04-18 |
| 基金项目:国家自然科学基金（300200125）;霍英东基金（81038）;上海市卫生局科委基金（03JC14084）. |
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| Protective effect of peroxisome proliferator-activated receptor γ on TNFα-induced injury of inner medullary collecting duct cells |
| 林沁,顾勇,林善锬,LIN Qin,GU Yong,LIN Shan-yan |
| () |
| Abstract: |
| Objective：To observe the protective effect of peroxisome proliferator-activated receptor γ （PPARγ） on TNFα- induced injury of the inner medullary collecting duct （IMCD） cells. Methods： Cultured IMCD cells were randomly divided into blank control, TNFα stimulation, PPARγ transfection （pPPARγ-control） and PPARγ transfection ＋ TNFα stimulation groups. Stimulation was induced with 50 ng/ml TNFα for 24 h and mouse wild-type PPARγ plasmid was used to transfect IMCD cells. Cell supernatant MCP-1 or TGFβ1 was detected by ELISA method. Results： IMCD cells transfected with wild-type PPARγ plasmid had high expression of PPARγ mRNA and protein. The contents of MCP-1 and TGFβ1 in the supernatant were similar in blank control group and pPPARγ-control group. Compared with blank control group, TNFα stimulation group had decreased contents of MCP-1 and TGFβ1 in the supernatant （P〈0. 005）, but the contents of TNFα stimulation group were significantly higher than those of PPARγ transfection ＋ TNFα stimulation group （P〈0.05 and P（0. 005, respectively）. Conclusion： IMCD cells over expressing PPARγ have anti-inflammatory and anti-fibrosis effects. |
| Key words: peroxisome proliferator-activated receptor γ tumor necrosis factor-α inner medullary collecting duct cells |
