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| 海水吸入致大鼠急性肺损伤模型的建立 |
| 黄静霞,孙瑜,傅辰春,邓小明,卢建,HUANGJing-xia,SUNYu,FUChen-chun,DENGXiao-ming,LUJian |
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| 摘要: |
| 目的:建立海水吸入肺损伤动物模型.方法:24只大鼠随机分为对照组9只和模型组15只.模型组大鼠屏气9～10 s后吸入海水,4 ml/kg,分2次进行;对照组大鼠不吸入海水.于海水吸入前、海水吸入后10、30、60、120、240 min观察动脉血气和呼吸频率的变化,并记录动物的生存时间.4 h后处死动物,观察肺系数、支气管肺泡灌洗液(BALF)中总蛋白(TP)含量和白细胞计数以及肺组织形态学的变化.结果:与对照组相比,模型组大鼠吸入海水后呼吸频率显著增加,动脉血氧分压(PaO2)显著降低(P＜0.05),之后虽逐渐回升,但一直维持在较低的水平;肺系数和TP含量均显著升高(P＜0.05);白细胞计数显著增加(P＜0.05);肺组织形态学显示明显的肺水肿、肺泡萎陷和炎细胞浸润情况.结论:成功建立海水吸入肺损伤的动物模型. |
| 关键词: 模型,动物、海水、肺损伤 |
| DOI：10.3724/SP.J.1008.2006.00676 |
| 投稿时间：2006-01-04修订日期：2006-04-26 |
| 基金项目:全军医药卫生“十五”科研项目（01MA145）;上海市科委重点基础项目（03JC14002）. |
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| An acute lung injury model caused by seawater aspiration in rats |
| 黄静霞,孙瑜,傅辰春,邓小明,卢建,HUANG Jing-xia,SUN Yu,FU Chen-chun,DENG Xiao-ming,LU Jian |
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| Abstract: |
| Objective： To establish an acute lung injury model by seawater aspiration in rats. Methods： Twenty-four rats were randomly allocated into 2 groups： control group （n= 9） and model group （n= 15）. The acute lung injury model was established in rats by aspirating 2 ml seawater/kg body weight after 9-10 seconds of breath occlusion. The aspiration was repeated by the same means 2 rain later. Rats in control group aspirated no seawater. Arterial blood gases and respiratory rates were observed before aspiration and 10 min, 30 rain, 1 h, 2 h and 4 h after aspiration. The survival times of animal were also recorded. The rats were sacrificed 4 h after aspiration; the lung index, total protein （TP） contents, white blood cell （WBC） count in bronchoalveolar lavage fluid （BALF）, and the pulmonary histopathology were observed. Results： Compared with control group, model group had a significantly increased respiratory rate and a decreased PaO2 （P〈0.05）,which subsequently rose again and remained at a lower level. The model group also had a markedly increased lung index, TP contents and WBC counts （P〈 0.05）. Obvious edema, alveolar atrophy and inflammatory infiltration were found in model rats. Conclusion： An acute lung injury model by seawater aspiration has been successfuly established |
| Key words: model,animal seawater lung injury |
