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| 人喉癌组织中支原体感染的检测 |
| 赵舒薇,孙爱华,孙宝春,郎军添,邱杰,何金,强笔,王海青,英信江,叶青 |
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| (第二军医大学长征医院耳鼻咽喉科,上海,200003;解放军总医院附属304医院耳鼻咽喉科,北京,100037;长征医院病理科) |
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| 摘要: |
| 目的:检测、分析喉癌组织中支原体感染的情况,探讨支原体感染对喉癌发生、发展以及预后的影响.方法:应用免疫组织化学方法对214例喉不同病变及喉癌颈转移淋巴结组织标本中支原体感染情况进行检测,其中包括喉癌121例,喉癌前病变21例,声带息肉17例,癌旁正常喉组织14例,癌周对侧正常喉组织9例以及喉癌颈转移淋巴结32例,并对结果进行分析. 结果:人喉癌组织、喉癌颈转移淋巴结组织、喉癌前病变、声带息肉、癌旁正常喉组织、癌周对侧正常喉组织中抗猪鼻支原体抗体2G10反应阳性率分别为44.63﹪(54/121)、34.38﹪(11/32)、23.81﹪(5/21)、17.65﹪(3/17)、7.14﹪(1/14)、0﹪(0/9);人喉癌组织、喉癌颈转移淋巴结组织的2G10感染阳性率明显高于其他各组 (P＜0.05).中晚期喉癌(Ⅲ-Ⅳ期)中2G10感染明显高于早期喉癌(Ⅰ-Ⅱ期)[68.18﹪(45/66) vs 16.36﹪(9/55),P＜0.01].伴颈淋巴结转移喉癌组织2G10阳性率为62.50﹪(20/32),无颈淋巴结转移喉癌阳性率为35.96﹪(32/89),二者之间有显著差异(P＜0.01).2G10阴性Ⅲ-Ⅳ期喉癌病例3、5年生存率分别为85.71﹪(18/21)及84.21﹪(16/19),而2G10表达阳性Ⅲ-Ⅳ期喉癌病例3、5年生存率则分别为60.0﹪(27/45)及58.14﹪(25/43),2G10表达阳性病例3、5年生存率均明显低于2G10表达阴性病例(P＜0.05).结论:人喉癌组织支原体感染率较高,支原体感染与喉癌的发生、发展以及预后有一定的相关性. |
| 关键词: 喉肿瘤、支原体感染、预后 |
| DOI：10.3724/SP.J.1008.2006.00817 |
| 投稿时间：2005-12-21修订日期：2006-04-05 |
| 基金项目:国家自然科学基金(30371610). |
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| Detection of mycoplasma infection in human laryngeal carcinoma |
| ZHAO Shu-wei,SUN Ai-hua,SUN Bao-chun,LANG Jun-tian,QIU Jie,HE Jin,QIANG Bi,WANG Hai-qing,YING Xin-jiang,YE Qing |
| (第二军医大学长征医院耳鼻咽喉科,上海,200003;解放军总医院附属304医院耳鼻咽喉科,北京,100037) |
| Abstract: |
| Objective：To detect and analyze the mycoplasma infection in human laryngeal carcinoma and to explore the relationship between the infection with the development, progression and prognosis of laryngeal cancer. Methods： Immunohistochemical methods were employed to detect the mycoplasma infection in 214 specimens of different laryngeal lesions and cervical lymphatic tumors metastasized from laryngeal cancer, including 121 laryngeal cancer specimens, 21 laryngeal precancerous specimens, 17 vocal cord polypus specimens, 14 normal laryngeal tissues adjacent to cancer tissues, 9 normal laryngeal tissues opposite to the cancer, and 32 cervical lymphatic tumor specimens metastasized from laryngeal cancer. The infection results were subjected to statistical analysis. Results： The positive rates of mycoplasma 2G10 antibody in laryngeal carcinoma specimens, cervical lymphatic tumor specimens metastasized from laryngeal cancer, laryngeal precancerous specimens, vocal cord polypus specimens, normal laryngeal tissues adjacent to cancer tissues, and normal laryngeal tissues opposite to the cancer were 44.63% （54/121） ,34.38%（11/32） ,23.81%（5/21） ,17.65%（3/17） ,7.14%（1/14） ,and 0%（0/9）, respectively, with those of laryngeal carcinoma specimens and cervical lymphatic tumor specimens metastasized from laryngeal cancer significantly higher than those of other specimens（P〈0.05）. The positive rate of early stage laryngeal carcinoma （Ⅰ-Ⅱ stage, 16.36 % [9/55]） was significantly lower than that of advanced stage laryngeal carcinoma（ Ⅲ-Ⅳ, 68.18 % [45/66]）, （P〈0.01）. The positive rate of laryngeal carcinoma with cervical metastasis was higher （62.50%[20/32]） than that without cervical metastasis（35.96%[32/89]） ,（P〈0.01）. The 3- and 5-year survival rates of Ⅲ-Ⅳ stage patients positive of 2G10 antigen （60.0%[27/45], 58.14%[25/43]） were lower than those of patients negative of 2G10 antigen （85.71 % [18/21] ,84.21% （[16/19]）, （P〈0.05）. Conclusion： Laryngeal carcinoma has a high infection rate of mycoplasma, which may be related with the development, progression and prognosis of laryngeal carcinoma. |
| Key words: laryngeal neoplasms mycoplasma infections prognosis |
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