| 摘要: |
| 目的:对二杈狗牙花根茎的化学成分进行分离及结构鉴定.方法:利用硅胶柱、凝胶柱及反相硅胶柱等色谱柱技术进行分离,根据理化性质及波谱学方法进行结构鉴定.结果:分离得到7个化合物:α-香树醇(α-amyrin,1),α-香树醇乙酸酯(α-amyrin acetate,2),α-香树醇棕榈酯(α-amyryl palmitate,3),蒲公英甾醇乙酸酯(taraxasterol acetate,4), 毛蕊异黄酮(calycosin,5), 芒柄花素(formononetin,6), farnisin (7,3'-dihydroxy-4'-methoxyflavone,7).结论:化合物1,3～7均为首次从本植物中分离得到, 化合物3～7为首次从该属植物中分得. |
| 关键词: 二杈狗牙花、分离和提纯、中药鉴定 |
| DOI：10.3724/SP.J.1008.2006.00892 |
| 投稿时间：2006-01-17修订日期：2006-05-30 |
| 基金项目:国家自然科学基金(20272081). |
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| Study on chemical constituents of Tabernaemontana divaricata |
| LIANG Shuang,CHEN Hai-sheng,DU Jing-ling,WANG Hou-peng,SHEN Yang,HUANG Min-zhu |
| (第二军医大学药学院天然药物化学教研室,上海,200433) |
| Abstract: |
| Objective：To extract and identify the chemical constituents in the roots and stems of Tabernaernontana divaricata. Methods： The solvent extraction approach and silica column chromatography were used for separation of the chemical constituents, and their structures were identified by physicochemical properties and spectra analysis. Results： Seven compounds were isolated and their structures were identified as α-amyrin （1）, α-amyrin acetate （2）, α-amyryl octadecanoate（3）, taraxasterol acetate （4）, calycosin （5）, formononetin （6） ,and farnisin （7）. Conclusion： Compound 1, 3, 4, 5, 6 and 7 are isolated from this plant for the first time, and Compound 3, 4, 5, 6 and 7 are isolated from Tabernaemontan genus for the first time |
| Key words: Tabernaemontana divaricata isolation ＆ purification TCD identification |
