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| 人类重组免疫球蛋白λ轻链022与TOM1基因在2型糖尿病胰岛素抵抗患者网膜脂肪组织的表达 |
| 赵卫民,邹俊杰,石勇铨,邹秀兰,郑骄阳,刘志民 |
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| (第二军医大学长征医院内分泌科,上海,200003;湖北省宜昌市第一人民医院内分泌科,宜昌,443000) |
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| 摘要: |
| 目的:探讨人类重组免疫球蛋白λ轻链(HSIGVL)022和TOM1基因在2型糖尿病胰岛素抵抗(T2DM-IR)患者网膜脂肪组织表达水平并鉴定HSIGVL022蛋白表达.方法:取2型糖尿病伴胰岛素抵抗(T2DM-IR组)与非糖尿病无胰岛素抵抗者(对照组)网膜脂肪组织,用荧光定量RT-PCR技术测定HSIGVL022和TOM1 mRNA的表达量,用免疫组化方法鉴定HSIGVL022蛋白表达.结果:荧光定量RT-PCR显示HSIGVL022和TOM1 mRNA的表达量T2DM-IR组和对照组分别为(34 140±6 160)、(4 440±617)和(5 930±661)、(1 360±82)拷贝/每百万看家基因(P＜0.05);HSIGVL022较TOM1表达量高(P＜0.01),且与HOMA-IR呈直线相关(P＜0.05);免疫组化显示HSIGVL022蛋白在T2DM-IR组和对照组阳性率分别为(12.43±2.41)﹪、(2.31±0.48)﹪,强阳性率分别为(6.62±1.69)﹪、(2.12±0.34)﹪,P＜0.05.结论:HSIGVL022和TOM1在人网膜脂肪组织中均有表达,HSIGVL022表达量较高;在T2DM-IR者中均呈上调表达,HSIGVL022蛋白表达相应增高;HSIGVL022 mRNA表达量与HOMA-IR呈直线相关;这2条基因可能与T2DM-IR有关. |
| 关键词: 糖尿病,2型、胰岛素抵抗、脂肪组织、免疫球蛋白类,λ链、TOM1 |
| DOI：10.3724/SP.J.1008.2006.00953 |
| 投稿时间：2006-01-20修订日期：2006-07-25 |
| 基金项目:上海市卫生局医学发展基金重点项目[2001 ZD002(3)]. |
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| Expression of recombined human immunoglobulin λ light chain 022 and TOM1 mRNA in omental adipose tissues of patients with type 2 diabetes and insulin resistance |
| ZHAO Wei-min,ZOU Jun-jie,SHI Yong-quan,ZOU Xiu-lan,ZHENG Jiao-yang,LIU Zhi-min |
| (第二军医大学长征医院内分泌科,上海,200003;湖北省宜昌市第一人民医院内分泌科,宜昌,443000) |
| Abstract: |
| Objective：To investigate the expression of recombined human immunoglobulin X light chain 022 and TOM1 gene and to determine the protein expression of HSIGVL022 in omental adipose tissues of patients with type 2 diabetes and insulin resistance（T2DM-IR）. Methods： Fat tissues from greater omentum of T2DM-IR patients and normal controls were obtained. The mRNA levels of recombined human immunoglobulin λ light chain 022 and TOM1 were measured by fluorescent quantitative RTPCR;expression of HSIGVL022 protein was identified by immunohistochemistry. Results： The mRNA levels of HSIGVL022 and TOM1 in T2DM-IR patients were （34 140±6 160） copy/million house-keeping genes and （4 440±617） copy/million housekeeping genes, respectively; those in control group were （5 930-661） copy/million house-keeping genes and （1 360±82） cop- y/million house-keeping genes,respectively. There were significant difference between the 2 groups （P〈0. 05）. The mRNA level of HSIGVL022 was significantly higher than that of TOMI（P〈0.01） and was linearly correlated with homeostatic model assessment-insulin resistance（HOMA-IR） index（P〈0.05）. The positive rates of HSIGVL022 protein in T2DM-IR and control group were （12.43±2.41） % and （2.31±0.48） %, respectively; the strong positive rates were （6.62! 1.69）% and （2.12±0.34）%, respectively（P〈0.05）. Conclusion： Both HSIGVL022 and TOM1 are expressed in the fat tissues of greater omenturn,with the level of HSIGVL022 higher than that of TOM1. The 2 genes are up-regulated in patients with insulin resistance and the HSIGVL022 protein increases correspondingly. The mRNA level of HSIGVL022 is linearly correlated with HOMA-IR index. The 2 genes are possibly related with insulin resistance in type 2 diabetes |
| Key words: diabetes mellitus,type 2 insulin resistance adipose tissue immunoglobulins lambda chain TOM1 |
